{"corpus_id":23604283,"paper_sha":"1a82cf93a7b003273aa897b4ce947e5d6345f429","doi":"10.1016/S0166-6851(98)00118-2","arxiv_id":null,"pmid":9879882,"pmcid":null,"mag_id":1964561451,"dblp_id":null,"acl_id":null,"title":"Identification of a nuclear protein in Trypanosoma brucei with homology to RNA-binding proteins from cis-splicing systems.","year":1998,"publication_date":"1998-11-30","venue":"Molecular and biochemical parasitology (Print)","journal":{"name":"Molecular and biochemical parasitology","pages":"\n          1-11\n        ","volume":"97 1-2"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, Non-U.S. Gov't","Research Support, U.S. Gov't, P.H.S."],"s2_fields_of_study":["Biology","Medicine"],"reference_count":41,"citation_count":36,"influential_citation_count":1,"is_open_access":false,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Amino Acid Sequence","mj":false,"ui":"D000595"},{"d":"Animals","mj":false,"ui":"D000818"},{"d":"Base Sequence","mj":false,"ui":"D001483"},{"d":"Blotting, Western","mj":false,"ui":"D015153"},{"d":"Cloning, Molecular","mj":false,"ui":"D003001"},{"d":"Fluorescent Antibody Technique, Indirect","mj":false,"ui":"D019084"},{"d":"Genes, Protozoan","mj":false,"ui":"D017125"},{"d":"Molecular Sequence Data","mj":false,"ui":"D008969"},{"d":"Nuclear Proteins","mj":false,"qs":[{"q":"analysis","mj":false,"ui":"Q000032"},{"q":"genetics","mj":true,"ui":"Q000235"}],"ui":"D009687"},{"d":"Open Reading Frames","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"}],"ui":"D016366"},{"d":"Polymerase Chain Reaction","mj":false,"qs":[{"q":"methods","mj":false,"ui":"Q000379"}],"ui":"D016133"},{"d":"Protozoan Proteins","mj":false,"qs":[{"q":"analysis","mj":false,"ui":"Q000032"},{"q":"genetics","mj":true,"ui":"Q000235"}],"ui":"D015800"},{"d":"RNA, Protozoan","mj":false,"qs":[{"q":"analysis","mj":false,"ui":"Q000032"}],"ui":"D016053"},{"d":"RNA-Binding Proteins","mj":false,"qs":[{"q":"analysis","mj":false,"ui":"Q000032"},{"q":"genetics","mj":true,"ui":"Q000235"}],"ui":"D016601"},{"d":"Sequence Alignment","mj":false,"ui":"D016415"},{"d":"Trans-Splicing","mj":false,"qs":[{"q":"genetics","mj":true,"ui":"Q000235"}],"ui":"D020040"},{"d":"Trypanosoma brucei brucei","mj":false,"qs":[{"q":"chemistry","mj":false,"ui":"Q000737"},{"q":"genetics","mj":true,"ui":"Q000235"}],"ui":"D014346"}],"chemicals":[{"n":"Nuclear Proteins","ui":"D009687","reg":"0"},{"n":"Protozoan Proteins","ui":"D015800","reg":"0"},{"n":"RNA, Protozoan","ui":"D016053","reg":"0"},{"n":"RNA-Binding Proteins","ui":"D016601","reg":"0"},{"n":"RRM1 protein, Trypanosoma brucei","ui":"C117851","reg":"0"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":null,"s2_open_access_landing_url":null,"s2_open_access_license":null,"s2_open_access_status":null,"pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Gene expression in trypanosomes is controlled at the level of pre-mRNA maturation via trans-splicing and polyadenylation and through changes in mRNA stability. To identify the trans- acting factors involved in this regulation, we have used a degenerate PCR approach to clone genes encoding the RNA recognition motif (RRM) consensus. We have identified a single-copy gene encoding a protein (designated RRM1) which contains three consensus RRM motifs, two tandem copies of a retroviral gag-like CCHC 'zinc finger' and an arginine-serine (RS) rich region. Western blotting indicates that RRM1 is expressed in both procyclic and bloodstream-form trypanosomes and has an apparent mobility on SDS-PAGE of ca. 70 Kd. RRM1 is localized in the trypanosome nucleus in substructures which may be functionally analogous to the 'speckles' associated with cis-splicing in higher eukaryotic cells. The structure of RRM1, its pattern of expression and its intracellular location suggest that it may play a role in trans-splicing.","claims":[{"public_id":"cl_e5300d5c58602d02a6f1dbc062744317","status":"active","text":"A single-copy gene encoding the nuclear protein RRM1 was identified in Trypanosoma brucei using a degenerate PCR approach targeting RNA recognition motif consensus sequences.","confidence":0.96,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_e5300d5c58602d02a6f1dbc062744317"},{"public_id":"cl_007cb3748e4ddf5797975f142b0e1baf","status":"active","text":"RRM1 contains three consensus RNA recognition motif domains, two tandem retroviral gag-like CCHC zinc fingers, and an arginine-serine-rich region.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous 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