{"corpus_id":24686342,"paper_sha":"66b0edeceeae2bf319b439f60047dd7449d9a20a","doi":"10.1073/PNAS.77.3.1311","arxiv_id":null,"pmid":6246487,"pmcid":"PMC348484","mag_id":2011147958,"dblp_id":null,"acl_id":null,"title":"Transforming gene product of Rous sarcoma virus phosphorylates tyrosine","year":1980,"publication_date":"1980-03-01","venue":"Proceedings of the National Academy of Sciences of the United States of America","journal":{"name":"Proceedings of the National Academy of Sciences","pages":"1311 - 1315","volume":"77"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, U.S. Gov't, P.H.S."],"s2_fields_of_study":["Biology","Medicine"],"reference_count":27,"citation_count":1819,"influential_citation_count":55,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Avian Sarcoma Viruses","mj":false,"qs":[{"q":"enzymology","mj":true,"ui":"Q000201"},{"q":"genetics","mj":false,"ui":"Q000235"}],"ui":"D001358"},{"d":"Cell Transformation, Viral","mj":true,"ui":"D002472"},{"d":"Cells, Cultured","mj":false,"ui":"D002478"},{"d":"Genes, Viral","mj":false,"ui":"D005814"},{"d":"Molecular Weight","mj":false,"ui":"D008970"},{"d":"Phosphorylation","mj":false,"ui":"D010766"},{"d":"Protein Kinases","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D011494"},{"d":"Substrate Specificity","mj":false,"ui":"D013379"},{"d":"Tyrosine","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D014443"},{"d":"Viral Proteins","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D014764"}],"chemicals":[{"n":"Viral Proteins","ui":"D014764","reg":"0"},{"n":"Tyrosine","ui":"D014443","reg":"42HK56048U"},{"n":"Protein Kinases","ui":"D011494","reg":"EC 2.7.-"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"https://www.pnas.org/doi/pdf/10.1073/pnas.77.3.1311","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/66b0edeceeae2bf319b439f60047dd7449d9a20a","s2_open_access_license":null,"s2_open_access_status":"BRONZE","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"The protein kinase activity associated with pp60src, the transforming protein of Rous sarcoma virus, was found to phosphorylate tyrosine when assayed in an immunoprecipitate. Despite the fact that a protein kinase with this activity has not been described before, several observations suggest that pp60src also phosphorylates tyrosine in vivo. First, chicken cells transformed by Rous sarcoma virus contain as much as 8-fold more phosphotyrosine than do uninfected cells. Second, phosphotyrosine is present in pp60src itself, at one of the two sites of phosphorylation. Third, phosphotyrosine is present in the 50,000-dalton phosphoprotein that coprecipitates with pp60src extracted from transformed chicken cells. We infer from these observations that pp60src is a novel protein kinase and that the modification of proteins via the phosphorylation of tyrosine is essential to the malignant transformation of cells by Rous sarcoma virus. pp60sarc, the closely related cellular homologue of viral pp60src, is present in all vertebrate cells. This normal cellular protein, obtained from both chicken and human cells, also phosphorylated tyrosine when assayed in an immunoprecipitate. This is additional evidence of the functional similarity of these structurally related proteins and demonstrates that all uninfected vertebrate cells contain at least one protein kinase that phosphorylates tyrosine.","claims":[{"public_id":"cl_39f3aed352150ebf7d7c63b9ce9f98b6","status":"active","text":"Transformed chicken cells contain up to eightfold more phosphotyrosine than uninfected cells, and phosphotyrosine is detected in pp60src and in a 50,000-dalton phosphoprotein that coprecipitates with pp60src.","confidence":0.96,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_39f3aed352150ebf7d7c63b9ce9f98b6"},{"public_id":"cl_50e75984bc3f4c1a1f1f7f9885cbba23","status":"active","text":"pp60sarc from chicken and human cells also phosphorylates tyrosine in an immunoprecipitate assay, showing that uninfected vertebrate cells contain at least one tyrosine-phosphorylating protein kinase.","confidence":0.97,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_50e75984bc3f4c1a1f1f7f9885cbba23"},{"public_id":"cl_2dfe801a2752be785b196d702903c91d","status":"active","text":"pp60src is inferred to be a novel protein kinase, and tyrosine phosphorylation is inferred to be essential for malignant transformation by Rous sarcoma virus.","confidence":0.9,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_2dfe801a2752be785b196d702903c91d"},{"public_id":"cl_ade19069790c94f48c315121a2759fad","status":"active","text":"pp60src-associated protein kinase activity phosphorylates tyrosine in an immunoprecipitate 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