{"corpus_id":25110928,"paper_sha":"9a27311fbceb5d65c865a209e23b74a4adc9314c","doi":"10.3791/1385","arxiv_id":null,"pmid":19718012,"pmcid":"3149908","mag_id":1977671739,"dblp_id":null,"acl_id":null,"title":"Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure","year":2009,"publication_date":"2009-08-28","venue":"Journal of Visualized Experiments","journal":{"name":"Journal of Visualized Experiments : JoVE","pages":null,"volume":null},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Video-Audio Media"],"s2_fields_of_study":["Medicine"],"reference_count":0,"citation_count":48,"influential_citation_count":0,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Animals","mj":false,"ui":"D000818"},{"d":"Female","mj":false,"ui":"D005260"},{"d":"Heme Oxygenase-1","mj":false,"qs":[{"q":"analysis","mj":true,"ui":"Q000032"},{"q":"biosynthesis","mj":false,"ui":"Q000096"},{"q":"genetics","mj":false,"ui":"Q000235"}],"ui":"D051547"},{"d":"Luciferases","mj":false,"qs":[{"q":"analysis","mj":false,"ui":"Q000032"}],"ui":"D008156"},{"d":"Luminescent Measurements","mj":false,"qs":[{"q":"methods","mj":true,"ui":"Q000379"}],"ui":"D008163"},{"d":"Medicine, Traditional","mj":true,"ui":"D008519"},{"d":"Mice","mj":false,"ui":"D051379"},{"d":"Mice, Transgenic","mj":false,"ui":"D008822"},{"d":"Skin","mj":false,"qs":[{"q":"blood supply","mj":false,"ui":"Q000098"},{"q":"enzymology","mj":false,"ui":"Q000201"}],"ui":"D012867"},{"d":"Up-Regulation","mj":false,"ui":"D015854"}],"chemicals":[{"n":"Luciferases","ui":"D008156","reg":"EC 1.13.12.-"},{"n":"Heme Oxygenase-1","ui":"D051547","reg":"EC 1.14.14.18"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"https://europepmc.org/articles/pmc3149908?pdf=render","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/9a27311fbceb5d65c865a209e23b74a4adc9314c","s2_open_access_license":"CCBY","s2_open_access_status":"GREEN","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Gua Sha is a traditional Chinese folk therapy that employs skin scraping to cause subcutaneous microvascular blood extravasation and bruises. The protocol for bioluminescent optical imaging of HO-1-luciferase transgenic mice reported in this manuscript provides a rapid in vivo assay of the upregulation of the heme oxygenase-1 (HO-1) gene expression in response to the Gua Sha procedure. HO-1 has long been known to provide cytoprotection against oxidative stress. The upregulation of HO-1, assessed by the bioluminescence output, is thought to represent an antioxidative response to circulating hemoglobin products released by Gua Sha. Gua Sha was administered by repeated strokes of a smooth spoon edge over lubricated skin on the back or other targeted body part of the transgenic mouse until petechiae (splinter hemorrhages) or ecchymosis (bruises) indicative of extravasation of blood from subcutaneous capillaries was observed. After Gua Sha, bioluminescence imaging sessions were carried out daily for several days to follow the dynamics of HO-1 expression in multiple internal organs.","claims":[{"public_id":"cl_b6b48425822635f8128e7c476046507b","status":"active","text":"Bioluminescent optical imaging in HO-1-luciferase transgenic mice provides a rapid in vivo assay of heme oxygenase-1 gene upregulation in response to the Gua Sha procedure.","confidence":0.97,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_b6b48425822635f8128e7c476046507b"},{"public_id":"cl_b4abbc0f56f3b9906ebbd7f7ae41382f","status":"active","text":"Gua Sha can be administered in mice by repeated strokes of a smooth spoon edge over lubricated skin until petechiae or ecchymosis appears as evidence of subcutaneous capillary blood extravasation.","confidence":0.95,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_b4abbc0f56f3b9906ebbd7f7ae41382f"},{"public_id":"cl_af4757b0c02faef5ff8ac6eda3c77bf7","status":"active","text":"HO-1 upregulation is interpreted as an antioxidative response to circulating hemoglobin products released by Gua Sha.","confidence":0.78,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_af4757b0c02faef5ff8ac6eda3c77bf7"},{"public_id":"cl_ae4a8346cbd61dfbb37982ef52a8ea0a","status":"active","text":"Post-Gua Sha bioluminescence imaging over several days tracks the dynamics of HO-1 expression in multiple internal organs.","confidence":0.9,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_ae4a8346cbd61dfbb37982ef52a8ea0a"}],"concepts":[{"public_id":"co_07fe72df32b7923ecfc8dc0856cecacd","status":"active","name":"petechiae","description":"Small pinpoint hemorrhages observed on the skin after scraping.","types":["clinical sign","observation"],"aliases":["splinter hemorrhages"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_07fe72df32b7923ecfc8dc0856cecacd"},{"public_id":"co_174ff224c680e3daf7ec91462986c452","status":"active","name":"multiple internal organs","description":"Several internal tissues examined for changes in HO-1 expression.","types":["anatomical structures"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_174ff224c680e3daf7ec91462986c452"},{"public_id":"co_1c210fd88d4d3971f73dc870e704d94a","status":"active","name":"HO-1 upregulation","description":"An increase in heme oxygenase-1 gene expression.","types":["molecular response"],"aliases":["heme oxygenase-1 upregulation"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_1c210fd88d4d3971f73dc870e704d94a"},{"public_id":"co_29a4909cc6f12de785648a691908a245","status":"active","name":"HO-1 expression","description":"The level of heme oxygenase-1 gene expression measured by bioluminescence output.","types":["molecular expression"],"aliases":["heme oxygenase-1 expression"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_29a4909cc6f12de785648a691908a245"},{"public_id":"co_3241b9861e5c1b7ac56d6efeb6582c2d","status":"active","name":"antioxidative response","description":"A biological response associated with defense against oxidative stress.","types":["biological response"],"aliases":["antioxidant response"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_3241b9861e5c1b7ac56d6efeb6582c2d"},{"public_id":"co_46d0f86301051efb2a1379bdbfc9faac","status":"active","name":"bioluminescence imaging sessions","description":"Repeated imaging measurements collected daily after the procedure to follow signal changes over time.","types":["measurement procedure"],"aliases":["imaging sessions"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_46d0f86301051efb2a1379bdbfc9faac"},{"public_id":"co_7b56044400c891a53b491f52d818f46e","status":"active","name":"circulating hemoglobin products","description":"Hemoglobin-derived substances released into circulation after blood extravasation.","types":["biochemical substance"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_7b56044400c891a53b491f52d818f46e"},{"public_id":"co_962e673f9402cc5ad2ef5f26df17f1d9","status":"active","name":"bioluminescent optical imaging","description":"An in vivo imaging method that detects light emitted from luciferase activity.","types":["method"],"aliases":["bioluminescence imaging","optical imaging"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_962e673f9402cc5ad2ef5f26df17f1d9"},{"public_id":"co_a0f8b2e2624178c22a959c662e66c512","status":"active","name":"HO-1-luciferase transgenic mice","description":"Transgenic mice engineered to report heme oxygenase-1 promoter activity through luciferase expression.","types":["animal model","reporter model"],"aliases":["HO-1-luc mice"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_a0f8b2e2624178c22a959c662e66c512"},{"public_id":"co_a206484393365440fed1fd2d295bd3ce","status":"active","name":"ecchymosis","description":"Bruised skin discoloration caused by bleeding into the subcutaneous tissue.","types":["clinical sign","observation"],"aliases":["bruises"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_a206484393365440fed1fd2d295bd3ce"},{"public_id":"co_dc03bf210f3561c281c687fd298991c3","status":"active","name":"Gua Sha procedure","description":"A traditional Chinese therapy that uses repeated skin scraping to induce local bruising and microvascular blood extravasation.","types":["therapy","procedure"],"aliases":["Gua Sha"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_dc03bf210f3561c281c687fd298991c3"}],"external_ids":{"DOI":"10.3791/1385","ArXiv":null,"PubMed":19718012,"PubMedCentral":"3149908","MAG":1977671739,"DBLP":null,"ACL":null},"open_access":{"is_open_access":true,"pdf_url":"https://europepmc.org/articles/pmc3149908?pdf=render","landing_url":"https://www.semanticscholar.org/paper/9a27311fbceb5d65c865a209e23b74a4adc9314c","source":"semantic_scholar","pdf_url_source":"semantic_scholar_open_access_pdf","license":"CCBY","status":"GREEN","reason":null},"reference_availability":{"status":"unknown","references_indexed":false,"full_text_available":false,"full_text_source":null,"count_basis":"semantic_scholar_metadata","extraction_status":"not_applicable","reason":null},"source":{"provider":"episteme2","base_corpus":"semantic_scholar_dump","freshness_mode":"unknown","basis":["semantic_scholar_metadata","postgres_metadata"],"limits":["paper metadata is based on indexed upstream scholarly datasets","claims and concepts are available only for extracted papers","absence of claims or concepts means no extracted graph data is available in this response"],"status":"available","degraded":false,"degraded_reasons":[],"diagnostics":{"status":"available","degraded":false,"degraded_reasons":[],"metadata_status":"available","graph_status":"available","abstract_status":"available"},"source_flags":5},"paper_id":632313,"paper_uid":"21d17e82-5d5d-41e6-9205-d9c7a490bd11","canonical_identity":{"paper_id":632313,"paper_uid":"21d17e82-5d5d-41e6-9205-d9c7a490bd11","identity_status":"available","lookup_basis":"semantic_scholar_external_id","compatibility_path":"corpus_id"},"url":"https://sah.borca.ai/papers/25110928"}