{"corpus_id":251122321,"paper_sha":"83e2edde9749a3f3ad82bebb3a08519d5fe61633","doi":"10.3390/ijms23158168","arxiv_id":null,"pmid":35897744,"pmcid":"9332151","mag_id":null,"dblp_id":null,"acl_id":null,"title":"STABILON, a Novel Sequence Motif That Enhances the Expression and Accumulation of Intracellular and Secreted Proteins","year":2022,"publication_date":"2022-07-25","venue":"International Journal of Molecular Sciences","journal":{"name":"International Journal of Molecular Sciences","pages":null,"volume":"23"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article"],"s2_fields_of_study":["Biology","Medicine","Chemistry"],"reference_count":36,"citation_count":12,"influential_citation_count":0,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Amino Acid Motifs","mj":false,"ui":"D020816"},{"d":"Animals","mj":false,"ui":"D000818"},{"d":"Drosophila","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D004330"},{"d":"Drosophila Proteins","mj":true,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D029721"},{"d":"Mammals","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D008322"},{"d":"Proteasome Endopeptidase Complex","mj":true,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D046988"},{"d":"Proteolysis","mj":false,"ui":"D059748"},{"d":"Ubiquitin","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D025801"}],"chemicals":[{"n":"Drosophila Proteins","ui":"D029721","reg":"0"},{"n":"Ubiquitin","ui":"D025801","reg":"0"},{"n":"Proteasome Endopeptidase Complex","ui":"D046988","reg":"EC 3.4.25.1"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"https://www.mdpi.com/1422-0067/23/15/8168/pdf?version=1658741835","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/83e2edde9749a3f3ad82bebb3a08519d5fe61633","s2_open_access_license":"CCBY","s2_open_access_status":"GOLD","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"The dynamic balance of transcriptional and translational regulation together with degron-controlled proteolysis shapes the ever-changing cellular proteome. While a large variety of degradation signals has been characterized, our knowledge of cis-acting protein motifs that can in vivo stabilize otherwise short-lived proteins is very limited. We have identified and characterized a conserved 13-mer protein segment derived from the p54/Rpn10 ubiquitin receptor subunit of the Drosophila 26S proteasome, which fulfills all the characteristics of a protein stabilization motif (STABILON). Attachment of STABILON to various intracellular as well as medically relevant secreted model proteins resulted in a significant increase in their cellular or extracellular concentration in mammalian cells. We demonstrate that STABILON acts as a universal and dual function motif that, on the one hand, increases the concentration of the corresponding mRNAs and, on the other hand, prevents the degradation of short-lived fusion proteins. Therefore, STABILON may lead to a breakthrough in biomedical recombinant protein production.","claims":[{"public_id":"cl_53ccc35d717302730f5f60e31e08da8b","status":"active","text":"A conserved 13-mer segment from the Drosophila p54/Rpn10 ubiquitin receptor subunit functions as a protein stabilization motif, STABILON.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_53ccc35d717302730f5f60e31e08da8b"},{"public_id":"cl_7a5f97fa3b06e813b12ef878c20cb61f","status":"active","text":"Attaching STABILON to diverse intracellular and secreted model proteins significantly increases their cellular or extracellular concentration in mammalian cells.","confidence":0.97,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous 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