{"corpus_id":25182434,"paper_sha":"1574455fa14911a5faac54b6c4665eb20446f904","doi":"10.1016/s0021-9258(18)43328-5","arxiv_id":null,"pmid":6167568,"pmcid":null,"mag_id":1529295649,"dblp_id":null,"acl_id":null,"title":"Recognition of nucleophile-treated alpha 2-macroglobulin by the alveolar macrophage alpha-macroglobulin . protease complex receptor.","year":1981,"publication_date":"1981-08-10","venue":"Journal of Biological Chemistry","journal":{"name":"The Journal of biological chemistry","pages":"\n          7705-7\n        ","volume":"256 15"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, U.S. Gov't, P.H.S."],"s2_fields_of_study":["Biology","Medicine","Chemistry"],"reference_count":0,"citation_count":53,"influential_citation_count":0,"is_open_access":false,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Ammonium Chloride","mj":false,"qs":[{"q":"pharmacology","mj":false,"ui":"Q000494"}],"ui":"D000643"},{"d":"Ammonium Sulfate","mj":false,"qs":[{"q":"pharmacology","mj":false,"ui":"Q000494"}],"ui":"D000645"},{"d":"Animals","mj":false,"ui":"D000818"},{"d":"Cell Membrane","mj":false,"qs":[{"q":"drug effects","mj":false,"ui":"Q000187"},{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D002462"},{"d":"Kinetics","mj":false,"ui":"D007700"},{"d":"Low Density Lipoprotein Receptor-Related Protein-1","mj":false,"ui":"D026503"},{"d":"Macrophages","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D008264"},{"d":"Peptide Hydrolases","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D010447"},{"d":"Rabbits","mj":false,"ui":"D011817"},{"d":"Receptors, Immunologic","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D011971"},{"d":"Trypsin","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D014357"},{"d":"alpha-Macroglobulins","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D000511"}],"chemicals":[{"n":"Low Density Lipoprotein Receptor-Related Protein-1","ui":"D026503","reg":"0"},{"n":"Receptors, Immunologic","ui":"D011971","reg":"0"},{"n":"alpha-Macroglobulins","ui":"D000511","reg":"0"},{"n":"Ammonium Chloride","ui":"D000643","reg":"01Q9PC255D"},{"n":"Peptide Hydrolases","ui":"D010447","reg":"EC 3.4.-"},{"n":"Trypsin","ui":"D014357","reg":"EC 3.4.21.4"},{"n":"Ammonium Sulfate","ui":"D000645","reg":"SU46BAM238"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":null,"s2_open_access_landing_url":null,"s2_open_access_license":null,"s2_open_access_status":null,"pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Rabbit alveolar macrophages exhibit high affinity surface receptors which recognize alpha 2-macroglobulin . protease complexes but not native alpha 2- macroglobulin. Binding of alpha 2-macroglobulin . protease complexes to surface receptors is independent of the protease used to form the complex. In this communication, we demonstrate that treatment of human alpha 2-macroglobulin with nucleophilic agents (methyl amine, ammonium salts) converts native alpha 2-macroglobulin into a form recognized by the surface receptor for alpha 2-macroglobulin protease complexes. Analysis of the concentration dependency of ligand binding revealed that the surface receptor did not distinguish between nucleophile-treated alpha 2-macroglobulin and alpha 2-macroglobulin . protease complexes. These results are consistent with the hypothesis that proteases or nucleophilic agents effect the hydrolysis of an internal thiol-ester bond (Tack, B. F., Harrison, R. A., Janatova, J., Thomas, M. L., and Prahl, J. W. (1980) Proc. Natl. Acad. Sci. U. S. A. 77, 5764-5768), leading to an alteration in alpha 2-macroglobulin conformation. The altered conformation results in recognition of the alpha 2-macroglobulin by surface receptors.","claims":[{"public_id":"cl_6ade9c97c63a8b79ba72389f66cf5471","status":"active","text":"Ligand binding analysis indicates that the receptor does not distinguish between nucleophile-treated alpha 2-macroglobulin and alpha 2-macroglobulin–protease complexes.","confidence":0.95,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_6ade9c97c63a8b79ba72389f66cf5471"},{"public_id":"cl_6bba994e492beef855ccb799112b6923","status":"active","text":"Nucleophile treatment with methyl amine or ammonium salts converts human alpha 2-macroglobulin into a form recognized by the alveolar macrophage alpha-macroglobulin–protease complex receptor.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_6bba994e492beef855ccb799112b6923"},{"public_id":"cl_a645df3bfd5059804cb284a438cf551c","status":"active","text":"Recognition of alpha 2-macroglobulin by surface receptors is consistent with hydrolysis of an internal thiol-ester bond causing a conformational change.","confidence":0.9,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_a645df3bfd5059804cb284a438cf551c"}],"concepts":[{"public_id":"co_0def1cf9f1231c57d6893edf6f85b77d","status":"active","name":"conformational change","description":"An alteration in the protein's three-dimensional structure.","types":["molecular change"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_0def1cf9f1231c57d6893edf6f85b77d"},{"public_id":"co_1624ee59b274c9263825b5bc867442e0","status":"active","name":"alpha 2-macroglobulin protease complexes","description":"Complexes formed between alpha 2-macroglobulin and proteases that are recognized by the receptor.","types":["protein complex"],"aliases":["alpha 2-macroglobulin-protease complexes"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_1624ee59b274c9263825b5bc867442e0"},{"public_id":"co_2bbfef0f3e300b89da46487d23f0e47c","status":"active","name":"human alpha 2-macroglobulin","description":"A human plasma protein that can exist in native or altered receptor-recognized forms.","types":["protein"],"aliases":["human α2-macroglobulin","alpha 2-macroglobulin"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_2bbfef0f3e300b89da46487d23f0e47c"},{"public_id":"co_2d4af5d98d0dfce14a19eb58cc2edca9","status":"active","name":"nucleophile-treated alpha 2-macroglobulin","description":"Alpha 2-macroglobulin altered by nucleophilic reagents so that it is recognized by the receptor.","types":["protein form"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_2d4af5d98d0dfce14a19eb58cc2edca9"},{"public_id":"co_45d68bc7a57fdbaf4df4b0bc7b0757b6","status":"active","name":"ligand binding analysis","description":"Concentration-dependent measurement of receptor-ligand interactions.","types":["analysis method"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_45d68bc7a57fdbaf4df4b0bc7b0757b6"},{"public_id":"co_6631eb5ef19b5e7b786a9b81e4c35c0e","status":"active","name":"alveolar macrophage alpha-macroglobulin protease complex receptor","description":"A high-affinity surface receptor on rabbit alveolar macrophages that binds alpha-macroglobulin complexes.","types":["receptor"],"aliases":["alpha-macroglobulin protease complex receptor"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_6631eb5ef19b5e7b786a9b81e4c35c0e"},{"public_id":"co_9dfe55810a83bb22ae69fb97d8bc689d","status":"active","name":"internal thiol-ester bond","description":"A bond within alpha 2-macroglobulin proposed to undergo hydrolysis during activation.","types":["chemical bond"],"aliases":["thiol-ester bond"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_9dfe55810a83bb22ae69fb97d8bc689d"},{"public_id":"co_a96b328e87d3c62a2c4a7dd909baaf0d","status":"active","name":"nucleophilic agents","description":"Chemical reagents such as methyl amine and ammonium salts used to alter alpha 2-macroglobulin.","types":["chemical agent"],"aliases":["methyl amine","ammonium salts"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_a96b328e87d3c62a2c4a7dd909baaf0d"},{"public_id":"co_f35b3254c478cdde9279ac5505d407e6","status":"active","name":"surface receptors","description":"Cell-surface proteins that mediate recognition of altered alpha 2-macroglobulin forms.","types":["receptor"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_f35b3254c478cdde9279ac5505d407e6"}],"external_ids":{"DOI":"10.1016/s0021-9258(18)43328-5","ArXiv":null,"PubMed":6167568,"PubMedCentral":null,"MAG":1529295649,"DBLP":null,"ACL":null},"open_access":{"is_open_access":false,"pdf_url":null,"landing_url":"https://sah.borca.ai/papers/25182434","source":null,"pdf_url_source":null,"license":null,"reason":"pdf_url_not_indexed"},"reference_availability":{"status":"unknown","references_indexed":false,"full_text_available":false,"full_text_source":null,"count_basis":"semantic_scholar_metadata","extraction_status":"not_applicable","reason":null},"source":{"provider":"episteme2","base_corpus":"semantic_scholar_dump","freshness_mode":"unknown","basis":["semantic_scholar_metadata","postgres_metadata"],"limits":["paper metadata is based on indexed upstream scholarly datasets","claims and concepts are available only for extracted papers","absence of claims or concepts means no extracted graph data is available in this response"],"status":"available","degraded":false,"degraded_reasons":[],"diagnostics":{"status":"available","degraded":false,"degraded_reasons":[],"metadata_status":"available","graph_status":"available","abstract_status":"available"},"source_flags":5},"paper_id":630913,"paper_uid":"c96fd277-4a85-41a0-8590-8f1ab5eb64ec","canonical_identity":{"paper_id":630913,"paper_uid":"c96fd277-4a85-41a0-8590-8f1ab5eb64ec","identity_status":"available","lookup_basis":"semantic_scholar_external_id","compatibility_path":"corpus_id"},"url":"https://sah.borca.ai/papers/25182434"}