{"corpus_id":25533347,"paper_sha":"5fabc773735e4f4de72d2586261c09bb819c51b4","doi":"10.1074/JBC.270.9.4563","arxiv_id":null,"pmid":7876226,"pmcid":null,"mag_id":1999157304,"dblp_id":null,"acl_id":null,"title":"Modulation of the ATPase Activity of the Molecular Chaperone DnaK by Peptides and the DnaJ and GrpE Heat Shock Proteins (*)","year":1995,"publication_date":"1995-03-03","venue":"Journal of Biological Chemistry","journal":{"name":"The Journal of Biological Chemistry","pages":"4563 - 4569","volume":"270"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, U.S. Gov't, P.H.S."],"s2_fields_of_study":["Biology","Medicine","Chemistry"],"reference_count":29,"citation_count":131,"influential_citation_count":8,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Adenosine Triphosphatases","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D000251"},{"d":"Amino Acid Sequence","mj":false,"ui":"D000595"},{"d":"Bacterial Proteins","mj":false,"qs":[{"q":"physiology","mj":true,"ui":"Q000502"}],"ui":"D001426"},{"d":"Bacteriophage lambda","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"}],"ui":"D010582"},{"d":"DNA Replication","mj":false,"qs":[{"q":"drug effects","mj":false,"ui":"Q000187"}],"ui":"D004261"},{"d":"DNA, Viral","mj":false,"qs":[{"q":"biosynthesis","mj":false,"ui":"Q000096"}],"ui":"D004279"},{"d":"Enzyme Activation","mj":false,"ui":"D004789"},{"d":"Escherichia coli","mj":false,"qs":[{"q":"enzymology","mj":false,"ui":"Q000201"},{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D004926"},{"d":"Escherichia coli Proteins","mj":true,"ui":"D029968"},{"d":"HSP40 Heat-Shock Proteins","mj":false,"ui":"D050956"},{"d":"HSP70 Heat-Shock Proteins","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D018840"},{"d":"Heat-Shock Proteins","mj":false,"qs":[{"q":"physiology","mj":true,"ui":"Q000502"}],"ui":"D006360"},{"d":"Molecular Sequence Data","mj":false,"ui":"D008969"},{"d":"Peptides","mj":false,"qs":[{"q":"chemistry","mj":false,"ui":"Q000737"},{"q":"physiology","mj":true,"ui":"Q000502"}],"ui":"D010455"}],"chemicals":[{"n":"Bacterial Proteins","ui":"D001426","reg":"0"},{"n":"DNA, Viral","ui":"D004279","reg":"0"},{"n":"DnaJ protein, E coli","ui":"C494635","reg":"0"},{"n":"Escherichia coli Proteins","ui":"D029968","reg":"0"},{"n":"GrpE protein, Bacteria","ui":"C068113","reg":"0"},{"n":"GrpE protein, E coli","ui":"C489400","reg":"0"},{"n":"HSP40 Heat-Shock Proteins","ui":"D050956","reg":"0"},{"n":"HSP70 Heat-Shock Proteins","ui":"D018840","reg":"0"},{"n":"Heat-Shock Proteins","ui":"D006360","reg":"0"},{"n":"Peptides","ui":"D010455","reg":"0"},{"n":"Adenosine Triphosphatases","ui":"D000251","reg":"EC 3.6.1.-"},{"n":"dnaK protein, E coli","ui":"C063842","reg":"EC 3.6.1.-"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"http://www.jbc.org/article/S0021925818828137/pdf","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/5fabc773735e4f4de72d2586261c09bb819c51b4","s2_open_access_license":"CCBY","s2_open_access_status":"HYBRID","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Previous studies have demonstrated that the Escherichia coli DnaK, DnaJ, and GrpE heat shock proteins participate in the initiation of bacteriophage λ DNA replication by mediating the required disassembly of a preinitiation nucleoprotein structure that is formed at the phage replication origin. To gain some understanding in a simpler system of how the DnaJ and GrpE cochaperonins influence the activity of DnaK, we have examined the effect of the cochaperonins on the weak intrinsic ATPase activity of the molecular chaperone DnaK in the presence and absence of peptide effectors. We have found that random sequence peptide chains of 8 or 9 amino acid residues in length yield optimal (10-fold) activation of the DnaK ATPase, whereas peptides with 5 or fewer residues fail to stimulate the ATPase of this bacterial hsp70 homologue. Furthermore, we have discovered that those peptides that interact best with DnaK, as judged by their KA as activators of ATP hydrolysis by DnaK, also act as strong inhibitors of λ DNA replication in vitro. The inhibitory effect of peptides on λ DNA replication was overcome by increasing the concentration of DnaK in the replication system. Diminished inhibition was also found when the replication system was supplemented with GrpE cochaperonin, a protein known to increase the effectiveness of DnaK action in λ DNA replication. These and other results suggest that the peptide-binding site of DnaK is required for its function in λ DNA replication. Apparently, peptides sequester free DnaK protein and block λ DNA replication by reducing the amount of DnaK that is free to mediate disassembly of nucleoprotein preinitiation structures. In related studies, we have found that DnaJ, like short peptides, activates the intrinsic ATPase activity of DnaK. DnaJ, however, is substantially more potent in this regard, since it activates DnaK at concentrations 1000-fold below those required for a peptide of random sequence. By itself, the GrpE cochaperonin has no effect on the peptide-independent ATPase activity of DnaK, but GrpE does vigorously stimulate the peptide-dependent ATPase of the DnaK chaperone. Under steady-state conditions, the Vmax of ATP hydrolysis by DnaK was elevated approximately 40-fold by the presence of GrpE and saturating levels of peptides.","claims":[{"public_id":"cl_4cbe49680d9daa4d9571073c2d487cca","status":"active","text":"DnaJ activates the intrinsic ATPase activity of DnaK and does so at concentrations about 1000-fold lower than those required for a random-sequence peptide.","confidence":0.99,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_4cbe49680d9daa4d9571073c2d487cca"},{"public_id":"cl_60f6093ec32bb5cd312fb61bab125164","status":"active","text":"GrpE has no effect on peptide-independent DnaK ATPase activity but vigorously stimulates peptide-dependent ATPase activity, raising the steady-state Vmax of ATP hydrolysis by about 40-fold with saturating peptides.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_60f6093ec32bb5cd312fb61bab125164"},{"public_id":"cl_b5ff80595747d67c054e83b42ce60b05","status":"active","text":"Peptides that bind DnaK most strongly also strongly inhibit lambda DNA replication in vitro.","confidence":0.95,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_b5ff80595747d67c054e83b42ce60b05"},{"public_id":"cl_1acf5c6910092de8d2906854e268abae","status":"active","text":"Random-sequence peptides of 8 or 9 amino acid residues produce optimal, about 10-fold activation of the DnaK ATPase, whereas peptides with 5 or fewer residues do not stimulate ATPase 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