{"corpus_id":28352997,"paper_sha":"c718280106b0aab466ebeae84d30e324081b4a4e","doi":"10.1016/s0021-9258(19)45849-3","arxiv_id":null,"pmid":5002684,"pmcid":null,"mag_id":1517095082,"dblp_id":null,"acl_id":null,"title":"Phosphoenolpyruvate-dependent fructose phosphorylation in photosynthetic bacteria.","year":1971,"publication_date":"1971-12-25","venue":"Journal of Biological Chemistry","journal":{"name":"The Journal of biological chemistry","pages":"\n          7819-21\n        ","volume":"246 24"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article"],"s2_fields_of_study":["Biology","Medicine","Environmental Science"],"reference_count":0,"citation_count":57,"influential_citation_count":2,"is_open_access":false,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Carbon Isotopes","mj":false,"ui":"D002247"},{"d":"Chromatography, Gel","mj":false,"ui":"D002850"},{"d":"Fructose","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D005632"},{"d":"Fructosephosphates","mj":false,"qs":[{"q":"biosynthesis","mj":false,"ui":"Q000096"}],"ui":"D005636"},{"d":"Molecular Weight","mj":false,"ui":"D008970"},{"d":"Oxidative Phosphorylation","mj":false,"ui":"D010085"},{"d":"Phosphoenolpyruvate","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D010728"},{"d":"Phosphorus Isotopes","mj":false,"ui":"D010759"},{"d":"Phosphotransferases","mj":false,"qs":[{"q":"isolation & purification","mj":false,"ui":"Q000302"}],"ui":"D010770"},{"d":"Rhodobacter sphaeroides","mj":false,"qs":[{"q":"cytology","mj":false,"ui":"Q000166"},{"q":"enzymology","mj":false,"ui":"Q000201"},{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D012242"},{"d":"Rhodopseudomonas","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D012241"},{"d":"Rhodospirillum","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D012246"},{"d":"Rhodospirillum rubrum","mj":false,"qs":[{"q":"cytology","mj":false,"ui":"Q000166"},{"q":"enzymology","mj":false,"ui":"Q000201"},{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D012247"},{"d":"Subcellular Fractions","mj":false,"qs":[{"q":"enzymology","mj":false,"ui":"Q000201"}],"ui":"D013347"}],"chemicals":[{"n":"Carbon Isotopes","ui":"D002247","reg":"0"},{"n":"Fructosephosphates","ui":"D005636","reg":"0"},{"n":"Phosphorus Isotopes","ui":"D010759","reg":"0"},{"n":"Fructose","ui":"D005632","reg":"30237-26-4"},{"n":"Phosphoenolpyruvate","ui":"D010728","reg":"73-89-2"},{"n":"Phosphotransferases","ui":"D010770","reg":"EC 2.7.-"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":null,"s2_open_access_landing_url":null,"s2_open_access_license":null,"s2_open_access_status":null,"pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Abstract The non-sulfur purple photosynthetic bacteria, Rhodospirillum rubrum and Rhodopseudomonas spheroides, utilize fructose via a pathway initiated by an enzyme system that catalyzes the transfer of phosphate from phosphoenolpyruvate to the 1-hydroxyl of fructose. Two protein fractions, both required for phosphorylating activity, were separated. One is tightly associated with the membrane fraction, while the other, found in this fraction in the crude extract, was solubilized by extraction with water, and exhibits a molecular weight near 200,000. The enzyme system differs from the P-enolpyruvate-dependent phosphotransferase systems isolated from other bacteria.","claims":[{"public_id":"cl_aa52f7e93729d5016191deff9a6c541f","status":"active","text":"One protein fraction is tightly associated with the membrane fraction, while the other is water-soluble and has a molecular weight near 200,000.","confidence":0.92,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_aa52f7e93729d5016191deff9a6c541f"},{"public_id":"cl_3795460c5174b05070acc2dd2a4f2039","status":"active","text":"Rhodospirillum rubrum and Rhodopseudomonas spheroides utilize fructose through a pathway initiated by an enzyme system that transfers phosphate from phosphoenolpyruvate to the 1-hydroxyl of fructose.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous 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