{"corpus_id":33905084,"paper_sha":"c3416a7a100629fd44edff03a85ad8bf3303235f","doi":"10.1016/s0021-9258(19)44659-0","arxiv_id":null,"pmid":5082128,"pmcid":null,"mag_id":1595078971,"dblp_id":null,"acl_id":null,"title":"Purification and properties of solubilized mitochondrial -aminolevulinic acid synthetase and comparison with the cytosol enzyme.","year":1972,"publication_date":"1972-11-10","venue":"Journal of Biological Chemistry","journal":{"name":"The Journal of biological chemistry","pages":"\n          6818-26\n        ","volume":"247 21"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article"],"s2_fields_of_study":["Biology","Medicine","Chemistry"],"reference_count":0,"citation_count":40,"influential_citation_count":0,"is_open_access":false,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"5-Aminolevulinate Synthetase","mj":true,"qs":[{"q":"antagonists & inhibitors","mj":false,"ui":"Q000037"},{"q":"isolation & purification","mj":false,"ui":"Q000302"}],"ui":"D000624"},{"d":"Acetyltransferases","mj":false,"ui":"D000123"},{"d":"Aminobutyrates","mj":false,"ui":"D000613"},{"d":"Animals","mj":false,"ui":"D000818"},{"d":"Antibodies","mj":false,"ui":"D000906"},{"d":"Chromatography, Affinity","mj":false,"ui":"D002846"},{"d":"Chromatography, Gel","mj":false,"ui":"D002850"},{"d":"Cross Reactions","mj":false,"ui":"D003429"},{"d":"Cytoplasm","mj":false,"qs":[{"q":"enzymology","mj":false,"ui":"Q000201"}],"ui":"D003593"},{"d":"Dithiothreitol","mj":false,"ui":"D004229"},{"d":"Freeze Drying","mj":false,"ui":"D005612"},{"d":"Guinea Pigs","mj":false,"ui":"D006168"},{"d":"Heme","mj":false,"ui":"D006418"},{"d":"Keto Acids","mj":false,"ui":"D007651"},{"d":"Liver","mj":false,"qs":[{"q":"cytology","mj":false,"ui":"Q000166"},{"q":"enzymology","mj":true,"ui":"Q000201"}],"ui":"D008099"},{"d":"Macromolecular Substances","mj":false,"ui":"D046911"},{"d":"Mitochondria, Liver","mj":false,"qs":[{"q":"enzymology","mj":true,"ui":"Q000201"}],"ui":"D008930"},{"d":"Molecular Weight","mj":false,"ui":"D008970"},{"d":"Porphyrias","mj":false,"qs":[{"q":"chemically induced","mj":false,"ui":"Q000139"},{"q":"enzymology","mj":false,"ui":"Q000201"},{"q":"pathology","mj":false,"ui":"Q000473"}],"ui":"D011164"},{"d":"Rabbits","mj":false,"qs":[{"q":"immunology","mj":false,"ui":"Q000276"}],"ui":"D011817"},{"d":"Rats","mj":false,"ui":"D051381"},{"d":"Sodium Chloride","mj":false,"ui":"D012965"},{"d":"Vibration","mj":false,"ui":"D014732"}],"chemicals":[{"n":"Aminobutyrates","ui":"D000613","reg":"0"},{"n":"Antibodies","ui":"D000906","reg":"0"},{"n":"Keto Acids","ui":"D007651","reg":"0"},{"n":"Macromolecular Substances","ui":"D046911","reg":"0"},{"n":"Heme","ui":"D006418","reg":"42VZT0U6YR"},{"n":"Sodium Chloride","ui":"D012965","reg":"451W47IQ8X"},{"n":"Acetyltransferases","ui":"D000123","reg":"EC 2.3.1.-"},{"n":"5-Aminolevulinate Synthetase","ui":"D000624","reg":"EC 2.3.1.37"},{"n":"Dithiothreitol","ui":"D004229","reg":"T8ID5YZU6Y"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":null,"s2_open_access_landing_url":null,"s2_open_access_license":null,"s2_open_access_status":null,"pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Abstract δ-Aminolevulinic acid synthetase extracted from porphyric liver mitochondria by sonication or freeze-drying was found to be excluded from Sephadex G-200, and other studies revealed that activity was associated with a large aggregate. In contrast, aminoacetone synthetase behaved as a soluble enzyme with a molecular weight of 64,000. By treating the aggregate with both sodium chloride and dithioerythritol, δ-aminolevulinic acid synthetase activity was released to yield a soluble form of enzyme of molecular weight 77,000, as determined by Sephadex gel chromatography. This form of the enzyme was purified 40-fold from rat liver mitochondria by a method involving affinity chromatography. Antibodies prepared against the purified mitochondrial enzyme cross-react with the cytosol enzyme, although Sephadex chromatography indicates its molecular weight to be 178,000. No enzymically inactive, immunologically reactive precursor of δ-aminolevulinic acid synthetase could be detected in normal rat liver. End product inhibition by hemin was found to be 50% at 10 µm.","claims":[{"public_id":"cl_14c2cc560d9ca794e7bb1d63285f9eab","status":"active","text":"Affinity chromatography enables 40-fold purification of the soluble mitochondrial enzyme from rat liver mitochondria.","confidence":0.95,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_14c2cc560d9ca794e7bb1d63285f9eab"},{"public_id":"cl_7fa210219dcfc168f27e8f8e57b9492f","status":"active","text":"Antibodies against the purified mitochondrial enzyme cross-react with the cytosol enzyme, which has an estimated molecular weight of 178,000.","confidence":0.94,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous 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