{"corpus_id":34682371,"paper_sha":"0f5e1ff966bea3da2243e6bf43ebeef72a1a486a","doi":"10.1074/JBC.M108195200","arxiv_id":null,"pmid":11641405,"pmcid":null,"mag_id":2041839984,"dblp_id":null,"acl_id":null,"title":"Orthogonality of Calcium Concentration and Ability of 4,5-Diaminofluorescein to Detect NO*","year":2002,"publication_date":"2002-01-04","venue":"Journal of Biological Chemistry","journal":{"name":"The Journal of Biological Chemistry","pages":"47 - 49","volume":"277"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article"],"s2_fields_of_study":["Medicine","Chemistry","Environmental Science"],"reference_count":25,"citation_count":94,"influential_citation_count":2,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Calcium","mj":false,"qs":[{"q":"pharmacology","mj":true,"ui":"Q000494"}],"ui":"D002118"},{"d":"Fluorescein","mj":true,"ui":"D019793"},{"d":"Fluorescence","mj":false,"ui":"D005453"},{"d":"Magnesium","mj":false,"qs":[{"q":"pharmacology","mj":false,"ui":"Q000494"}],"ui":"D008274"},{"d":"Nitric Oxide","mj":false,"qs":[{"q":"analysis","mj":true,"ui":"Q000032"}],"ui":"D009569"}],"chemicals":[{"n":"4,5-diaminofluorescein","ui":"C111205","reg":"0"},{"n":"Nitric Oxide","ui":"D009569","reg":"31C4KY9ESH"},{"n":"Magnesium","ui":"D008274","reg":"I38ZP9992A"},{"n":"Calcium","ui":"D002118","reg":"SY7Q814VUP"},{"n":"Fluorescein","ui":"D019793","reg":"TPY09G7XIR"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"http://www.jbc.org/article/S0021925820879794/pdf","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/0f5e1ff966bea3da2243e6bf43ebeef72a1a486a","s2_open_access_license":"CCBY","s2_open_access_status":"HYBRID","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"We have developed diaminofluoresceins (DAFs) and diaminorhodamines as fluorescent indicators for NO based on the specific reaction of the aromatic vicinal diamines with NO. Among them, 4,5-diaminofluorescein (DAF-2) is widely used for real-time biological imaging of NO in cultured cells or tissues by many researchers. Contrary to a recent report of divalent cation sensitivity and photoactivation of DAF-2 (Broillet, M. C., Randin, O., and Chatton, J. Y. (2001) FEBS Lett. 491, 227–232), our study using NO gas itself reveals that the reaction of DAF-2 and NO is completely independent of Ca2+ and Mg2+ at physiological concentrations. Ca2+ enhances not the conversion of DAF-2 into its fluorescent product (DAF-2 triazole) but the release of NO from NO donors. Therefore it is concluded that DAF-2 can provide reliable information on NO production in biological systems regardless of the dynamic changes of Ca2+concentration.","claims":[{"public_id":"cl_ee5cf6c999d6d17032694b420fd24bba","status":"active","text":"Ca2+ enhances the release of NO from NO donors, not the conversion of DAF-2 into its fluorescent product DAF-2 triazole.","confidence":0.9,"contributors":[{"id":32,"public_id":"7c402c1b98","public_label":"뀨 (7c402c1b98)","roles":["extraction"],"url":"https://sah.borca.ai/u/7c402c1b98"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_ee5cf6c999d6d17032694b420fd24bba"},{"public_id":"cl_420ff8be28244844ff9b4bf1e4d81505","status":"active","text":"DAF-2 can provide reliable information on NO production in biological systems regardless of dynamic changes in Ca2+ 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