{"corpus_id":38209738,"paper_sha":"6b8b6e1c134c5514155ef9f78f5e0795c89c5422","doi":"10.1074/jbc.M004467200","arxiv_id":null,"pmid":10934198,"pmcid":null,"mag_id":2156612041,"dblp_id":null,"acl_id":null,"title":"Functional Complementation of BLNK by SLP-76 and LAT Linker Proteins*","year":2000,"publication_date":"2000-10-20","venue":"Journal of Biological Chemistry","journal":{"name":"The Journal of Biological Chemistry","pages":"33116 - 33122","volume":"275"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, U.S. Gov't, P.H.S."],"s2_fields_of_study":["Biology","Medicine"],"reference_count":47,"citation_count":43,"influential_citation_count":0,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Adaptor Proteins, Signal Transducing","mj":true,"ui":"D048868"},{"d":"Animals","mj":false,"ui":"D000818"},{"d":"B-Lymphocytes","mj":false,"qs":[{"q":"immunology","mj":true,"ui":"Q000276"}],"ui":"D001402"},{"d":"Carrier Proteins","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"physiology","mj":true,"ui":"Q000502"}],"ui":"D002352"},{"d":"Cell Line","mj":false,"ui":"D002460"},{"d":"Humans","mj":false,"ui":"D006801"},{"d":"Jurkat Cells","mj":false,"ui":"D019169"},{"d":"Membrane Proteins","mj":true,"ui":"D008565"},{"d":"Mice","mj":false,"ui":"D051379"},{"d":"Mitogen-Activated Protein Kinase 1","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D019950"},{"d":"Mitogen-Activated Protein Kinases","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D020928"},{"d":"Monocytes","mj":false,"qs":[{"q":"immunology","mj":false,"ui":"Q000276"}],"ui":"D009000"},{"d":"Phosphoproteins","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"metabolism","mj":true,"ui":"Q000378"},{"q":"physiology","mj":true,"ui":"Q000502"}],"ui":"D010750"},{"d":"Receptors, Antigen, B-Cell","mj":false,"qs":[{"q":"physiology","mj":false,"ui":"Q000502"}],"ui":"D011947"},{"d":"Recombinant Proteins","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D011994"},{"d":"Signal Transduction","mj":false,"ui":"D015398"},{"d":"T-Lymphocytes","mj":false,"qs":[{"q":"immunology","mj":true,"ui":"Q000276"}],"ui":"D013601"},{"d":"Transfection","mj":false,"ui":"D014162"}],"chemicals":[{"n":"Adaptor Proteins, Signal Transducing","ui":"D048868","reg":"0"},{"n":"B cell linker protein","ui":"C113939","reg":"0"},{"n":"Carrier Proteins","ui":"D002352","reg":"0"},{"n":"LAT protein, human","ui":"C110810","reg":"0"},{"n":"Lat protein, mouse","ui":"C491734","reg":"0"},{"n":"Membrane Proteins","ui":"D008565","reg":"0"},{"n":"Phosphoproteins","ui":"D010750","reg":"0"},{"n":"Receptors, Antigen, B-Cell","ui":"D011947","reg":"0"},{"n":"Recombinant Proteins","ui":"D011994","reg":"0"},{"n":"SLP-76 signal Transducing adaptor proteins","ui":"C092947","reg":"0"},{"n":"Mitogen-Activated Protein Kinase 1","ui":"D019950","reg":"EC 2.7.11.24"},{"n":"Mitogen-Activated Protein Kinases","ui":"D020928","reg":"EC 2.7.11.24"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"http://www.jbc.org/article/S0021925820892072/pdf","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/6b8b6e1c134c5514155ef9f78f5e0795c89c5422","s2_open_access_license":"CCBY","s2_open_access_status":"HYBRID","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Recent studies have demonstrated a requirement for the SLP-76 (SH2 domain-containingleukocyte protein of 76 kDa) and LAT (linker for activation of Tcells) adaptor/linker proteins in T cell antigen receptor activation and T cell development as well as the BLNK (Bcell linker) linker protein in B cell antigen receptor (BCR) signal transduction and B cell development. Whereas the SLP-76 and LAT adaptor proteins are expressed in T, natural killer, and myeloid cells and platelets, BLNK is preferentially expressed in B cells and monocytes. Although BLNK is structurally homologous to SLP-76, BLNK interacts with a variety of downstream signaling proteins that interact directly with both SLP-76 and LAT. Here, we demonstrate that neither SLP-76 nor LAT alone is sufficient to restore the signaling deficits observed in BLNK-deficient B cells. Conversely, the coexpression of SLP-76 and LAT together restored BCR-inducible calcium responses as well as activation of all three families of mitogen-activated protein kinases. Together, these data suggest functional complementation of SLP-76 and LAT in T cell antigen receptor function with BLNK in BCR function.","claims":[{"public_id":"cl_21fad3fc0e1fec01abced5081e94de6b","status":"active","text":"Coexpression of SLP-76 and LAT together restores BCR-inducible calcium responses and activation of all three mitogen-activated protein kinase families in BLNK-deficient B cells.","confidence":0.9,"contributors":[{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["extraction"],"url":"https://sah.borca.ai/u/322360f1c1"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":136,"public_id":"3c2apqe3ut","public_label":"Anonymous 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paralleling their role in T cell antigen receptor function.","confidence":0.85,"contributors":[{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["extraction"],"url":"https://sah.borca.ai/u/322360f1c1"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":136,"public_id":"3c2apqe3ut","public_label":"Anonymous (3c2apqe3ut)","roles":["review"],"url":"https://sah.borca.ai/u/3c2apqe3ut"}],"url":"https://sah.borca.ai/claims/cl_fb1b112731ab29756ed1c9a909a73df9"}],"concepts":[{"public_id":"co_0bcfa92eee0b8b02f0f7122a2347f240","status":"active","name":"BCR-inducible calcium response","description":"A calcium signaling response induced by B cell antigen receptor engagement, used as a functional readout of BCR signaling in this study.","types":["outcome","measurement"],"aliases":[],"contributors":[{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale 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