{"corpus_id":38500962,"paper_sha":"3143ac29493d504834c52cfbdeffc6c2ae8269b2","doi":"10.1126/SCIENCE.2420009","arxiv_id":null,"pmid":2420009,"pmcid":null,"mag_id":2092368843,"dblp_id":null,"acl_id":null,"title":"Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells.","year":1986,"publication_date":"1986-04-04","venue":"Science","journal":{"name":"Science","pages":"\n          61-5\n        ","volume":"232 4746"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, U.S. Gov't, P.H.S."],"s2_fields_of_study":["Biology","Medicine"],"reference_count":35,"citation_count":1274,"influential_citation_count":22,"is_open_access":false,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Animals","mj":false,"ui":"D000818"},{"d":"Cell Differentiation","mj":false,"qs":[{"q":"drug effects","mj":false,"ui":"Q000187"}],"ui":"D002454"},{"d":"Cell Line","mj":false,"ui":"D002460"},{"d":"Colony-Forming Units Assay","mj":false,"ui":"D003114"},{"d":"Colony-Stimulating Factors","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"pharmacology","mj":true,"ui":"Q000494"}],"ui":"D003115"},{"d":"DNA","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D004247"},{"d":"Escherichia coli","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"}],"ui":"D004926"},{"d":"Genes","mj":false,"ui":"D005796"},{"d":"Granulocyte Colony-Stimulating Factor","mj":false,"ui":"D016179"},{"d":"Granulocytes","mj":false,"qs":[{"q":"physiology","mj":true,"ui":"Q000502"}],"ui":"D006098"},{"d":"Humans","mj":false,"ui":"D006801"},{"d":"Leukemia","mj":false,"qs":[{"q":"pathology","mj":true,"ui":"Q000473"}],"ui":"D007938"},{"d":"Leukemia, Myeloid","mj":false,"qs":[{"q":"pathology","mj":false,"ui":"Q000473"}],"ui":"D007951"},{"d":"Mice","mj":false,"ui":"D051379"},{"d":"Plasmids","mj":false,"ui":"D010957"},{"d":"Recombinant Proteins","mj":false,"qs":[{"q":"pharmacology","mj":true,"ui":"Q000494"}],"ui":"D011994"}],"chemicals":[{"n":"Colony-Stimulating Factors","ui":"D003115","reg":"0"},{"n":"Recombinant Proteins","ui":"D011994","reg":"0"},{"n":"Granulocyte Colony-Stimulating Factor","ui":"D016179","reg":"143011-72-7"},{"n":"DNA","ui":"D004247","reg":"9007-49-2"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":null,"s2_open_access_landing_url":null,"s2_open_access_license":null,"s2_open_access_status":null,"pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Experiments were conducted to isolate and characterize the gene and gene product of a human hematopoietic colony-stimulating factor with pluripotent biological activities. This factor has the ability to induce differentiation of a murine myelomonocytic leukemia cell line WEHI-3B(D+) and cells from patients with newly diagnosed acute nonlymphocytic leukemia (ANLL). A complementary DNA copy of the gene encoding a pluripotent human granulocyte colony-stimulating factor (hG-CSF) was cloned and expressed in Escherichia coli. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay. In addition, recombinant hG-CSF can support early erythroid colonies and mixed colony formation. Competitive binding studies done with 125I-labeled hG-CSF and cell samples from two patients with newly diagnosed human leukemias as well as WEHI-3B(D+) cells showed that one of the human leukemias (ANLL, classified as M4) and the WEHI-3B(D+) cells have receptors for hG-CSF. Furthermore, the murine WEHI-3B(D+) cells and human leukemic cells classified as M2, M3, and M4 were induced by recombinant hG-CSF to undergo terminal differentiation to macrophages and granulocytes. The secreted form of the protein produced by the bladder carcinoma cell line 5637 was found to be O-glycosylated and to have a molecular weight of 19,600.","claims":[{"public_id":"cl_52c6a47828f2d384ade8949a72814cf5","status":"active","text":"A cDNA encoding hG-CSF was cloned and expressed in Escherichia coli, yielding recombinant hG-CSF capable of supporting neutrophil proliferation in a CFU-GM assay.","confidence":0.95,"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale 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differentiation to macrophages and granulocytes in WEHI-3B(D+) cells and human leukemic cells classified as M2, M3, and M4.","confidence":0.94,"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/claims/cl_2417729125f717f19d0a327c772ae05f"},{"public_id":"cl_a0bf880022fa0fea86bc82824086db8e","status":"active","text":"Recombinant hG-CSF supports early erythroid colonies and mixed colony formation in addition to granulocyte-macrophage progenitors.","confidence":0.92,"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani 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