{"corpus_id":39199741,"paper_sha":"49fdae2b670cbe6c8b21ba77354f6911ace6730d","doi":"10.4049/jimmunol.145.12.4131","arxiv_id":null,"pmid":2258611,"pmcid":null,"mag_id":1499789927,"dblp_id":null,"acl_id":null,"title":"Identification of a soluble IL-2 receptor beta-chain from human lymphoid cell line cells.","year":1990,"publication_date":"1990-12-15","venue":"Journal of Immunology","journal":{"name":"Journal of immunology","pages":"\n          4131-5\n        ","volume":"145 12"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, Non-U.S. Gov't"],"s2_fields_of_study":["Biology","Medicine"],"reference_count":0,"citation_count":36,"influential_citation_count":1,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Humans","mj":false,"ui":"D006801"},{"d":"In Vitro Techniques","mj":false,"ui":"D066298"},{"d":"Interleukin-2","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D007376"},{"d":"Lymphocytes","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D008214"},{"d":"Molecular Weight","mj":false,"ui":"D008970"},{"d":"Precipitin Tests","mj":false,"ui":"D011233"},{"d":"Receptors, Interleukin-2","mj":false,"qs":[{"q":"chemistry","mj":false,"ui":"Q000737"},{"q":"immunology","mj":false,"ui":"Q000276"},{"q":"isolation & purification","mj":true,"ui":"Q000302"},{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D015375"},{"d":"Solubility","mj":false,"ui":"D012995"},{"d":"Tumor Cells, Cultured","mj":false,"qs":[{"q":"chemistry","mj":false,"ui":"Q000737"}],"ui":"D014407"}],"chemicals":[{"n":"Interleukin-2","ui":"D007376","reg":"0"},{"n":"Receptors, Interleukin-2","ui":"D015375","reg":"0"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"https://journals.aai.org/jimmunol/article-pdf/145/12/4131/1050546/4131.pdf","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/49fdae2b670cbe6c8b21ba77354f6911ace6730d","s2_open_access_license":null,"s2_open_access_status":"BRONZE","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"A clone was isolated from the human lymphoid cell line YT that displayed IL-2R beta, and was found to express much higher levels of IL-2R beta than the original cells. Combining cell surface iodination, affinity labeling of the released soluble protein, and fluorescence sandwich-ELISA for both IL-2 and IL-2.(soluble)(s)IL-2R beta reactants revealed the presence of IL-2-binding protein in the culture supernatant as soluble forms of IL-2R beta. By using the fluorescence sandwich-ELISA elevated levels of sIL-2R beta were measured in culture supernatants of human T cell leukemia virus I positive T cell lines. In addition to this constitutive production of sIL-2R beta, normal PBMC could release low levels of IL-2R beta by stimulation with PHA. In contrast, this was not found in certain human T cell leukemia virus I negative T cell, B cell and macrophage lines. Immunoprecipitation of the soluble protein with IL-2R beta-specific mAb characterized it as an apparent 50- to 55-kDa molecule that is distinct from the 45-kDa soluble IL-2R alpha. Moreover, 10 to 15% of the total cell surface molecules were released into culture supernatants. 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