{"corpus_id":6500748,"paper_sha":"fcf28aecc7e04121456b5b9aaa6623c2fe2eb8da","doi":"10.1085/JGP.108.1.1","arxiv_id":null,"pmid":8817380,"pmcid":"2229298","mag_id":2109003864,"dblp_id":null,"acl_id":null,"title":"Bay K 8644 reveals two components of L-type Ca2+ channel current in clonal rat pituitary cells","year":1996,"publication_date":"1996-07-01","venue":"The Journal of General Physiology","journal":{"name":"The Journal of General Physiology","pages":"1 - 11","volume":"108"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, Non-U.S. Gov't","Research Support, U.S. Gov't, P.H.S."],"s2_fields_of_study":["Biology","Medicine","Chemistry"],"reference_count":28,"citation_count":19,"influential_citation_count":0,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester","mj":false,"qs":[{"q":"pharmacology","mj":true,"ui":"Q000494"}],"ui":"D001498"},{"d":"Animals","mj":false,"ui":"D000818"},{"d":"Calcium Channels","mj":false,"qs":[{"q":"drug effects","mj":true,"ui":"Q000187"}],"ui":"D015220"},{"d":"Dose-Response Relationship, Drug","mj":false,"ui":"D004305"},{"d":"Membrane Potentials","mj":false,"qs":[{"q":"drug effects","mj":true,"ui":"Q000187"}],"ui":"D008564"},{"d":"Patch-Clamp Techniques","mj":false,"ui":"D018408"},{"d":"Pituitary Gland","mj":false,"qs":[{"q":"drug effects","mj":true,"ui":"Q000187"}],"ui":"D010902"},{"d":"Rats","mj":false,"ui":"D051381"}],"chemicals":[{"n":"Calcium Channels","ui":"D015220","reg":"0"},{"n":"3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester","ui":"D001498","reg":"71145-03-4"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"https://rupress.org/jgp/article-pdf/108/1/1/1769913/1.pdf","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/fcf28aecc7e04121456b5b9aaa6623c2fe2eb8da","s2_open_access_license":"CCBYNCSA","s2_open_access_status":"BRONZE","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Whole-cell L-type Ca2+ channel current was recorded in GH3 clonal rat pituitary cells using Ba2+ as a charge carrier. In the presence of the dihydropyridine agonist Bay K 8644, deactivation was best described by two exponential components with time constants of approximately 2 and approximately 8 ms when recorded at -40 mV. The slow component activated at more negative potentials than the fast component: Half- maximal activation for the slow and fast components occurred at approximately -15 and approximately 1 mV, respectively. The fast component was more sensitive to enhancement by racemic Bay K 8644 than the slow component: ED50fast = approximately 21 nM, ED50slow = approximately 74 nM. Thyrotropin-releasing hormone (TRH; 1 microM) inhibited the slow component by approximately 46%, whereas the fast component was inhibited by approximately 22%. TRH inhibition of total L- current showed some voltage dependence, but each Bay K 8644-revealed component of L-current was inhibited in a voltage-independent manner. Therefore, the apparent voltage dependence of TRH action is derived from complexities in channel gating rather than from relief of inhibition at high voltages. In summary, Bay K 8644-enhanced L-currents in GH3 cells consist of two components with different sensitivities to voltage, racemic Bay K 8644, and the neuropeptide TRH.","claims":[{"public_id":"cl_cda9a78f879ef87fb0b2958aa50209d6","status":"active","text":"Bay K 8644-enhanced L-type Ca2+ current in GH3 clonal rat pituitary cells consists of two kinetically distinct components with deactivation time constants of approximately 2 ms and approximately 8 ms at -40 mV.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_cda9a78f879ef87fb0b2958aa50209d6"},{"public_id":"cl_d7455c6c84df29ea915a9ce95035e5b9","status":"active","text":"TRH inhibits the slow component more strongly than the fast component, and the inhibition of each component is 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