{"corpus_id":9280967,"paper_sha":"f29e37928c7c4a1df7837a44eb52593eef78a2b4","doi":"10.1016/j.jconrel.2013.12.023","arxiv_id":null,"pmid":24384299,"pmcid":null,"mag_id":2006999603,"dblp_id":null,"acl_id":null,"title":"Development of anionic bubble lipopolyplexes for efficient and safe gene transfection with ultrasound exposure in mice.","year":2014,"publication_date":"2014-02-28","venue":"Journal of Controlled Release","journal":{"name":"Journal of controlled release : official journal of the Controlled Release Society","pages":"\n          24-34\n        ","volume":"176"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, Non-U.S. Gov't"],"s2_fields_of_study":["Medicine","Chemistry","Engineering"],"reference_count":57,"citation_count":27,"influential_citation_count":1,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Animals","mj":false,"ui":"D000818"},{"d":"Cell Line","mj":false,"ui":"D002460"},{"d":"Cell Survival","mj":false,"qs":[{"q":"drug effects","mj":false,"ui":"Q000187"}],"ui":"D002470"},{"d":"DNA","mj":false,"qs":[{"q":"administration & dosage","mj":false,"ui":"Q000008"},{"q":"chemistry","mj":false,"ui":"Q000737"}],"ui":"D004247"},{"d":"Female","mj":false,"ui":"D005260"},{"d":"Gene Expression","mj":false,"ui":"D015870"},{"d":"Gene Transfer Techniques","mj":true,"ui":"D018014"},{"d":"Humans","mj":false,"ui":"D006801"},{"d":"Kidney","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D007668"},{"d":"Liposomes","mj":false,"qs":[{"q":"chemistry","mj":false,"ui":"Q000737"}],"ui":"D008081"},{"d":"Liver","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D008099"},{"d":"Luciferases","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"}],"ui":"D008156"},{"d":"Mice","mj":false,"ui":"D051379"},{"d":"Mice, Inbred ICR","mj":false,"ui":"D008813"},{"d":"Polymers","mj":false,"qs":[{"q":"chemistry","mj":false,"ui":"Q000737"}],"ui":"D011108"},{"d":"Spleen","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D013154"},{"d":"Ultrasonics","mj":false,"qs":[{"q":"methods","mj":true,"ui":"Q000379"}],"ui":"D014465"}],"chemicals":[{"n":"Liposomes","ui":"D008081","reg":"0"},{"n":"Polymers","ui":"D011108","reg":"0"},{"n":"DNA","ui":"D004247","reg":"9007-49-2"},{"n":"Luciferases","ui":"D008156","reg":"EC 1.13.12.-"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"https://nagasaki-u.repo.nii.ac.jp/record/5152/files/JCR176_24.pdf","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/a8a355860b218f73ef9cf3e2d0424721b94a424a","s2_open_access_license":"CCBYNCND","s2_open_access_status":"GREEN","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Anionic bubble lipopolyplexes have been developed as anionic ultrasound (US)-responsive gene delivery carriers with biocompatible compounds for efficient and safe transfection in mice. The particles of the anionic bubble lipopolyplexes were approximately 450-600nm with an anionic surface charge. In the absence of US exposure, the bubble lipopolyplexes showed extremely low gene expression in the human vascular endothelial cell line EAhy926. The anionic bubble lipopolyplexes, however, delivered pDNA into cells without endocytosis and showed markedly high gene expression following US exposure. The anionic bubble lipopolyplexes showed little cytotoxicity in EAhy926 cells and little aggregation with erythrocytes. Following intravenous administration into mice, the anionic bubble lipopolyplexes showed high levels of gene expression in the liver, kidney, and spleen only after US exposure to the abdominal area. The level of gene expression in liver non-parenchymal cells was significantly higher than that in parenchymal cells. In addition, the anionic bubble lipopolyplexes did not show any severe hepatic toxicity and did not enhance the production of proinflammatory cytokines. Overall, we have succeeded in preparing anionic bubble lipopolyplexes for efficient and safe transfection with US exposure in mice.","claims":[{"public_id":"cl_7dcd75930227810887f75a2feeec3a53","status":"active","text":"After intravenous administration in mice, ultrasound exposure to the abdominal area produced high gene expression in the liver, kidney, and spleen, with higher expression in liver non-parenchymal cells than in parenchymal cells.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_7dcd75930227810887f75a2feeec3a53"},{"public_id":"cl_4ce7375b722d4040e979183e41f8cb18","status":"active","text":"Anionic bubble lipopolyplexes enabled markedly high gene expression after ultrasound exposure while showing extremely low expression without 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