MiR-339-5p inhibits breast cancer cell migration and invasion in vitro and may be a potential biomarker for breast cancer prognosis

BackgroundMicroRNAs (miRNAs) play an important role in the regulation of cell growth, differentiation, apoptosis, and carcinogenesis. Detection of their expression may lead to identifying novel markers for breast cancer.MethodsWe profiled miRNA expression in three breast cancer cell lines (MCF-7, MDA-MB-231, and MDA-MB-468) and then focused on one miRNA, miR-339-5p, for its role in regulation of tumor cell growth, migration, and invasion and target gene expression. We then analyzed miR-339-5p expression in benign and cancerous breast tissue specimens.ResultsA number of miRNAs were differentially expressed in these cancer cell lines. Real-time PCR indicated that miR-339-5p expression was downregulated in the aggressive cell lines MDA-MB-468 and MDA-MB-231 and in breast cancer tissues compared with benign tissues. Transfection of miR-339-5p oligonucleotides reduced cancer cell growth only slightly but significantly decreased tumor cell migration and invasion capacity compared with controls. Real-time PCR analysis showed that BCL-6, a potential target gene of miR-339-5p, was downregulated in MDA-MB-231 cells by miR-339-5p transfection. Furthermore, the reduced miR-339-5p expression was associated with an increase in metastasis to lymph nodes and with high clinical stages. Kaplan-Meier analyses found that the patients with miR-339-5p expression had better overall and relapse-free survivals compared with those without miR-339-5p expression. Cox proportional hazards analyses showed that miR-339-5p expression was an independent prognostic factor for breast cancer patients.ConclusionsMiR-339-5p may play an important role in breast cancer progression, suggesting that miR-339-5p should be further evaluated as a biomarker for predicting the survival of breast cancer patients.

• Publication year

  2010

• Venue

  BMC Cancer

• Publication date

  2010-10-09

• Fields of study

  Medicine

• Identifiers
  DOI 10.1186/1471-2407-10-542PMID 20932331PMCID 2958952
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Prognostic significance of differentially expressed miRNAs in esophageal cancer

  2011cited by this paper
• MiR-218 Inhibits Invasion and Metastasis of Gastric Cancer by Targeting the Robo1 Receptor

  2010cited by this paper
• BCL-6 Oncoprotein in Breast Cancer: Loss of Expression in Disease Progression

  2009cited by this paper
• MicroRNAs in Cancer.

  2009cited by this paper
• miRNAs in lung cancer - Studying complex fingerprints in patient's blood cells by microarray experiments

  2009cited by this paper
• MicroRNAs in cancer: small molecules with a huge impact.

  2009cited by this paper
• Breast cancer metastasis suppressor 1 up-regulates miR-146, which suppresses breast cancer metastasis.

  2009cited by this paper
• MicroRNAs: novel biomarkers for human cancer.

  2009cited by this paper
• Expression of miR-21 and its targets (PTEN, PDCD4, TM1) in flat epithelial atypia of the breast in relation to ductal carcinoma in situ and invasive carcinoma

  2009cited by this paper
• Genome analysis of the platypus reveals unique signatures of evolution

  2008cited by this paper
• Endogenous human microRNAs that suppress breast cancer metastasis

  2008cited by this paper
• Prognostic significance of MMP‐9 and TIMP‐1 serum and tissue expression in breast cancer

  2008cited by this paper
• Altered MicroRNA Expression in Cervical Carcinomas

  2008cited by this paper
• Suppression of cell growth and invasion by miR-205 in breast cancer

  2008cited by this paper
• MicroRNA-21 Targets the Tumor Suppressor Gene Tropomyosin 1 (TPM1)*

  2007cited by this paper
• Tumour invasion and metastasis initiated by microRNA-10b in breast cancer

  2007cited by this paper
• Coordinate Suppression of ERBB2 and ERBB3 by Enforced Expression of Micro-RNA miR-125a or miR-125b*

  2007cited by this paper
• The vascular endothelial growth factor receptor (VEGFR‐1) supports growth and survival of human breast carcinoma

  2006cited by this paper
• CXCR4 chemokine receptor mediates prostate tumor cell adhesion through alpha5 and beta3 integrins.

  2006cited by this paper
• CXCR4 Chemokine Receptor Mediates Prostate Tumor Cell Adhesion through α5 and β3 Integrins

  2006cited by this paper
• miRBase: microRNA sequences, targets and gene nomenclature

  2005cited by this paper
• Combinatorial microRNA target predictions

  2005cited by this paper
• MicroRNA functions in animal development and human disease

  2005cited by this paper
• MicroRNA biogenesis and cancer.

  2005cited by this paper
• Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets.

  2005cited by this paper
• miRNAs, cancer, and stem cell division.

  2005cited by this paper
• MicroRNAs: Critical Regulators of Development, Cellular Physiology and Malignancy

  2005cited by this paper
• Intensified Cloning Efforts Have Revealed Numer

  2004cited by this paper
• Specific peptide interference reveals BCL6 transcriptional and oncogenic mechanisms in B-cell lymphoma cells

  2004cited by this paper
• The BCL6 proto-oncogene suppresses p53 expression in germinal-centre B cells

  2004cited by this paper
• MicroRNAs and cancer.

  2003cited by this paper
• Protein expression of B-cell lymphoma gene 6 (BCL-6) in invasive breast cancer is associated with cyclin D1 and hypoxia-inducible factor-1α (HIF-1α)

  2003cited by this paper
• Protein expression of B-cell lymphoma gene 6 (BCL-6) in invasive breast cancer is associated with cyclin D1 and hypoxia-inducible factor-1alpha (HIF-1alpha).

  2003cited by this paper
• BCL-6 is expressed in breast cancer and prevents mammary epithelial differentiation

  2003cited by this paper
• BMC Cancer

  2003cited by this paper
• Expert Commentary.

  2002cited by this paper
• Involvement of chemokine receptors in breast cancer metastasis

  2001cited by this paper
• Pathological prognostic factors in breast cancer.

  1999cited by this paper
• Expression of the BCL6 gene in the pre- and postnatal mouse.

  1998cited by this paper
• pathological prognostic factors in breast cancer. I. The value of histological grade in breast cancer: experience from a large study with long‐term follow‐up

  1991cited by this paper
• Cancer statistics

  1989cited by this paper

• B-cell lymphoma 6 in breast cancer: molecular mechanisms and clinical significance

  2025cites this paper
• miR-221/222, miR-224, and miR-339 are Overexpressed as Potential Biomarkers in Follicular Thyroid Neoplasms

  2025cites this paper
• Exploring shared microRNA profiles in liquid-based cytology and plasma as biomarkers of high-grade intraepithelial lesions

  2025cites this paper
• Extracellular derived-exosomal micrornas in pancreatic cancer: investigating their diagnostic importance and potential targets for the prevention and treatment in pancreatic cancer

  2025cites this paper
• Ethanol modulates astrocyte activation and neuroinflammation via miR-339/NLRP6 inflammasome signaling.

  2024cites this paper
• Research progress on miR-339

  2024cites this paper
• MiR-339-5p Inhibits Ferroptosis by Promoting Autophagic Degradation of FTH1 Through Targeting ATG7 in Liver Cancer Cells

  2024cites this paper
• miRNA-126a plays important role in myoblast and endothelial cell interaction

  2023cites this paper
• MicroRNAs affecting the susceptibility of melanoma cells to CD8+ T cell‐mediated cytolysis

  2023cites this paper
• BTB protein family and human breast cancer: signaling pathways and clinical progress

  2023cites this paper
• Therapeutic Delivery of Tumor Suppressor miRNAs for Breast Cancer Treatment

  2023cites this paper
• Identification of differentially expressed miRNAs and mRNAs associated with the regulation of breast cancer via in silico and in vitro methods

  2023cites this paper
• Genome-wide post-transcriptional regulation of bovine mammary gland response to Streptococcus uberis

  2022cites this paper
• Relative refractoriness of breast cancer cells to tumour necrosis factor‐α induced necroptosis

  2022cites this paper
• Mechanisms of TP53 Pathway Inactivation in Embryonic and Somatic Cells—Relevance for Understanding (Germ Cell) Tumorigenesis

  2021cites this paper
• LncRNA MAFG-AS1 promotes the malignant phenotype of ovarian cancer by upregulating NFKB1-dependent IGF1

  2021cites this paper
• miR-340: A multifunctional role in human malignant diseases

  2021cites this paper
• The Profile of MicroRNA Expression and a Group of Genes in Breast Cancer: Relationship to Tumor Progression and Immunohistochemical Status

  2021cites this paper
• Genome-wide association study on milk production and somatic cell score for Thai dairy cattle using weighted single-step approach with random regression test-day model.

  2021cites this paper
• Identification of a six‐microRNA signature as a potential diagnostic biomarker in breast cancer tissues

  2021cites this paper
• The Profile of MicroRNA Expression and a Group of Genes in Breast Cancer: Relationship to Tumor Progression and Immunohistochemical Status

  2021cites this paper
• circGLI3 Inhibits Oxidative Stress by Regulating the miR-339-5p/VEGFA Axis in IPEC-J2 Cells

  2021cites this paper
• Reactivation of tumour suppressor in breast cancer by enhancer switching through NamiRNA network

  2021cites this paper
• Effect of encephalomyocarditis virus 3'Untranslated region on GFP transient expression: implications in recombinant protein production

  2020cites this paper
• Potential miRNAs for miRNA-Based Therapeutics in Breast Cancer

  2020cites this paper
• RP11‐81H3.2 promotes gastric cancer progression through miR‐339‐HNRNPA1 interaction network

  2020cites this paper
• The Role of microRNAs in Organismal and Skin Aging

  2020cites this paper
• Mechanisms of drugs-resistance in small cell lung cancer: DNA-related, RNA-related, apoptosis-related, drug accumulation and metabolism procedure

  2020cites this paper
• LncRNA ANRIL regulates cell proliferation and migration via sponging miR-339-5p and regulating FRS2 expression in atherosclerosis.

  2020cites this paper
• Circulating microRNAs in Early Breast Cancer Patients and Its Association With Lymph Node Metastases

  2020cites this paper
• Increased expression of LINC01510 predicts poor prognosis and promotes malignant progression in human non-small cell lung cancer.

  2019cites this paper
• Long non-coding RNA MALAT1 regulates BLCAP mRNA expression through binding to miR-339-5p and promotes poor prognosis in breast cancer

  2019cites this paper
• MicroRNA biomarkers associated with type 1 myocardial infarction in HIV positive individuals.

  2019cites this paper
• [Plasma miRNA-23a and miRNA-451 as candidate biomarkers for early diagnosis of nonsmall cell lung cancer: a case-control study].

  2019cites this paper
• The oncogenic impact of RNF2 on cell proliferation, invasion and migration through EMT on mammary carcinoma.

  2019cites this paper
• Global miRNA, lncRNA, and mRNA Transcriptome Profiling of Endometrial Epithelial Cells Reveals Genes Related to Porcine Reproductive Failure Caused by Porcine Reproductive and Respiratory Syndrome Virus

  2019cites this paper
• miRNA-339-5p Plays an Important Role in Invasion and Migration of Pancreatic Cancer Cells

  2019cites this paper
• Exosome-derived miR-339-5p mediates radiosensitivity by targeting Cdc25A in locally advanced esophageal squamous cell carcinoma

  2019cites this paper
• MicroRNA-339 inhibits human hepatocellular carcinoma proliferation and invasion via targeting ZNF689

  2019cites this paper
• Prognostic role of microRNAs in breast cancer: A systematic review

  2019cites this paper
• Paeonol attenuates inflammation by targeting HMGB1 through upregulating miR-339-5p

  2019cites this paper
• miR-339-5p inhibits lung adenocarcinoma invasion and migration by directly targeting BCL6

  2018cites this paper
• PROGNOSTIC IMPACT OF MICRORNA EXPRESSION IN BREAST CANCER

  2018cites this paper
• MiR‐339 depresses cell proliferation via directly targeting S‐phase kinase‐associated protein 2 mRNA in lung cancer

  2018cites this paper
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  2018cites this paper
• miR-339 Promotes Development of Stem Cell Leukemia/Lymphoma Syndrome via Downregulation of the BCL2L11 and BAX Proapoptotic Genes.

  2018cites this paper
• MicroRNA‐339‐3p alleviates inflammation and edema and suppresses pulmonary microvascular endothelial cell apoptosis in mice with severe acute pancreatitis‐associated acute lung injury by regulating Anxa3 via the Akt/mTOR signaling pathway

  2018cites this paper
• MiR-339 depresses cell proliferation via directly targeting S-phase kinase-associated protein 2 (Skp2) mRNA in lung cancer: MiR-339 restrains proliferation of NSCLC via Skp2

  2018cites this paper
• Increased glutamine anabolism sensitizes non-small cell lung cancer to gefitinib treatment

  2018cites this paper
• Profile and clinical implication of circular RNAs in human papillary thyroid carcinoma

  2018cites this paper
• MicroRNA‑339‑5p inhibits cell proliferation of acute myeloid leukaemia by directly targeting SOX4.

  2018cites this paper
• Deep sequencing of circulating exosomal microRNA allows non-invasive glioblastoma diagnosis

  2018cites this paper
• miR-124 regulates EMT based on ZEB2 target to inhibit invasion and metastasis in triple-negative breast cancer.

  2018cites this paper
• Application of serum microRNA-9-5p, 21-5p, and 223-3p combined with tumor markers in the diagnosis of non-small-cell lung cancer in Yunnan in southwestern China

  2018cites this paper
• Identification of circular RNAs as a promising new class of diagnostic biomarkers for human breast cancer

  2017cites this paper
• High expressions of BCL6 and Lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancer

  2017cites this paper
• miR-339-5p inhibits metastasis of non-small cell lung cancer by regulating the epithelial-to-mesenchymal transition.

  2017cites this paper
• miR ‐ 339 ‐ 5 p inhibits metastasis of non ‐ small cell lung cancer by regulating the epithelial ‐ to ‐ mesenchymal transition

  2017cites this paper
• MiR-23a modulates X-linked inhibitor of apoptosis-mediated autophagy in human luminal breast cancer cell lines

  2017cites this paper
• MicroRNA expression profiling of porcine mammary epithelial cells after challenge with Escherichia coli in vitro

  2017cites this paper
• Transcribed ultraconserved region 339 promotes carcinogenesis by modulating tumor suppressor microRNAs

  2017cites this paper
• MicroRNA-7 suppresses the homing and migration potential of human endothelial cells to highly metastatic human breast cancer cells

  2017cites this paper
• miR‐339‐5p downregulation contributes to Taxol resistance in small‐cell lung cancer by targeting α1,2‐fucosyltransferase 1

  2017cites this paper
• Circulating microRNA-339-5p and -21 in plasma as an early detection predictors of lung adenocarcinoma.

  2017cites this paper
• Gene expression profile analysis in response to α1,2-fucosyl transferase (FUT1) gene transfection in epithelial ovarian carcinoma cells

  2016cites this paper
• Prolactin Inhibits BCL6 Expression in Breast Cancer Cells through a MicroRNA-339-5p-Dependent Pathway

  2016cites this paper
• Effects of miR-339-5p on invasion and prognosis of hepatocellular carcinoma.

  2016cites this paper
• MicroRNAs in Breast Cancer and Their Value as Biomarkers

  2016cites this paper
• microRNAs in breast cancer: regulatory roles governing the hallmarks of cancer

  2016cites this paper
• Genetic variants in microRNA and microRNA biogenesis pathway genes and breast cancer risk among women of African ancestry

  2016cites this paper
• The PRL PTPs: Regulating Gene Expression to Reprogram the Cancer Cell

  2016cites this paper
• Lactogenic hormones alter cellular and extracellular microRNA expression in bovine mammary epithelial cell culture

  2016cites this paper
• miR-339-5p inhibits migration and invasion in ovarian cancer cell lines by targeting NACC1 and BCL6

  2016cites this paper
• The regulation of the p53/MDM2 feedback loop by microRNAs.

  2015cites this paper
• Peripheral Blood MicroRNA Expression Profiles in Alzheimer’s Disease: Screening, Validation, Association with Clinical Phenotype and Implications for Molecular Mechanism

  2015cites this paper
• Inferred miRNA activity identifies miRNA-mediated regulatory networks underlying multiple cancers

  2015cites this paper
• Integrated microRNA and mRNA responses to acute human left ventricular ischemia.

  2015cites this paper
• MicroRNA expression profiling and functional annotation analysis of their targets associated with the malignant transformation of oral leukoplakia.

  2015cites this paper
• High Expression Levels of miR-21 and miR-210 Predict Unfavorable Survival in Breast Cancer: A Systemic Review and Meta-Analysis

  2015cites this paper
• Mutual interaction between BCL6 and miRNAs contributing to the pathogenesis of various cancers

  2015cites this paper
• MicroRNA‐339, an epigenetic modulating target is involved in human gastric carcinogenesis through targeting NOVA1

  2015cites this paper
• Role of Non-coding RNAs in chemotherapeutic treatments

  2015cites this paper
• DNA Metiltransferaz-3b ve Metil Bağlayan Bölge Proteini-1’i Hedefleyen Mirna Gen Polimorfizmlerinin Akciğer Kanseri İle İlişkisinin Belirlenmesi

  2015cites this paper
• Modulation of Sodium Iodide Symporter-mediated Thyroidal Radioiodide Uptake by Small Molecule Inhibitors, Natural Plant-based Products and microRNAs

  2015cites this paper
• MicroRNA-based therapy and breast cancer: A comprehensive review of novel therapeutic strategies from diagnosis to treatment.

  2015cites this paper
• MicroRNA-7 Inhibits Tumor Metastasis and Reverses Epithelial-Mesenchymal Transition through AKT/ERK1/2 Inactivation by Targeting EGFR in Epithelial Ovarian Cancer

  2014cites this paper
• MiRNA expression in breast cancer varies with lymph node metastasis and other clinicopathologic features

  2014cites this paper
• Prognostic and Biological Significance of MicroRNA-127 Expression in Human Breast Cancer

  2014cites this paper
• miR-320a is an independent prognostic biomarker for invasive breast cancer

  2014cites this paper
• miR-339-5p inhibits cell migration and invasion in vitro and may be associated with the tumor-node-metastasis staging and lymph node metastasis of non-small cell lung cancer

  2014cites this paper
• 15-Deoxy-Δ12,14-prostaglandin J2 induces expression of 15-hydroxyprostaglandin dehydrogenase through Elk-1 activation in human breast cancer MDA-MB-231 cells.

  2014cites this paper
• MicroRNA Classifier and Nomogram for Metastasis Prediction in Colon Cancer

  2014cites this paper
• MicroRNA-339-5 p inhibits colorectal tumorigenesis through regulation of the MDM 2 / p 53 signaling

  2014influential citation
• Identification and characterization of novel and differentially expressed microRNAs in peripheral blood from healthy and mastitis Holstein cattle by deep sequencing.

  2014cites this paper
• B-cell lymphoma 6 protein stimulates oncogenicity of human breast cancer cells

  2014cites this paper
• Molecular mechanisms and tumour heterogeneity in breast cancer

  2014cites this paper
• miR-339-5p regulates the p53 tumor-suppressor pathway by targeting MDM2

  2014cites this paper
• miR-339-5p inhibits alcohol-induced brain inflammation through regulating NF-κB pathway.

  2014cites this paper
• Small molecule with big role: MicroRNAs in cancer metastatic microenvironments.

  2014cites this paper
• microRNA-339-5p modulates Na+/I- symporter-mediated radioiodide uptake.

  2014cites this paper