Leaf coloration is one of the most important and attractive characteristics of Ananas comosus var. bracteatus. The chimeric character is not stable during the in vitro tissue culturing. Many regenerated plants lost economic values for the loss of the chimeric character of leaves. In order to reveal the molecular mechanisms involved in the albino phenotype of the leaf cells, the physiological and transcriptional differences between complete white (CWh) and green (CGr) leaf cells of A. comosus var. bracteatus were analyzed. A total of 1,431 differentially expressed unigenes (DEGs) in CGr and CWh leaves were identified using RNA-seq. A comparison to the COG, GO and KEGG annotations revealed DEGs involved in chlorophyll biosynthesis, chloroplast development and photosynthesis. Furthermore, the measurement of main precursors of chlorophyll in the CWh leaves confirmed that the rate-limiting step in chlorophyll biosynthesis, and thus the cause of the albino phenotype of the white cells, was the conversion of pyrrole porphobilinogen (PBG) to uroporphyrinogen III (Uro III). The enzyme activity of porphobilinogen deaminase (PBGD) and uroporporphyrinogn III synthase (UROS), which catalyze the transition of PBG to Uro III, was significantly decreased in the CWh leaves. Our data showed the transcriptional differences between the CWh and CGr plants and characterized key steps in chlorophyll biosynthesis of the CWh leaves. These results contribute to our understanding of the mechanisms and regulation of pigment biosynthesis in the CWh leaf cells of A. comosus var. bracteatus.
Physiological Characterization and Comparative Transcriptome Analysis of White and Green Leaves of Ananas comosus var. bracteatus
Xia Li,S. Kanakala,Yehua He,X.-J. Zhong,Sanmiao Yu,Ruixue Li,Lingxia Sun,Jun Ma
Published 2017 in PLoS ONE
ABSTRACT
PUBLICATION RECORD
- Publication year
2017
- Venue
PLoS ONE
- Publication date
2017-01-17
- Fields of study
Biology, Medicine, Environmental Science
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- chlorophyll biosynthesis
The biochemical pathway that produces chlorophyll precursors in the leaf cells examined in this work.
- chloroplast development
The cellular developmental process that forms functional chloroplasts in the leaf tissue.
- complete white leaves
Leaf tissue of Ananas comosus var. bracteatus with complete white coloration that served as the albino comparison sample.
Aliases: CWh, CWh leaves, white leaves
- differentially expressed unigenes
Unigene transcripts whose expression differed between the compared leaf samples.
Aliases: DEGs
- photosynthesis
The light-driven carbon-fixation process carried out by chloroplasts in the leaf cells.
- porphobilinogen deaminase
An enzyme in the chlorophyll biosynthetic pathway that acts on porphobilinogen.
Aliases: PBGD
- rna-seq
High-throughput transcriptome sequencing used to compare gene expression between the leaf samples.
Aliases: RNA sequencing, transcriptome sequencing
- uroporphyrinogen iii synthase
An enzyme in the chlorophyll biosynthetic pathway that catalyzes formation of uroporphyrinogen III.
Aliases: UROS
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