Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis

The plant hormone auxin (indole-3-acetic acid, IAA) has a crucial role in plant development. Its spatiotemporal distribution is controlled by a combination of biosynthetic, metabolic and transport mechanisms. Four families of auxin transporters have been identified that mediate transport across the plasma or endoplasmic reticulum membrane. Here we report the discovery and the functional characterization of the first vacuolar auxin transporter. We demonstrate that WALLS ARE THIN1 (WAT1), a plant-specific protein that dictates secondary cell wall thickness of wood fibres, facilitates auxin export from isolated Arabidopsis vacuoles in yeast and in Xenopus oocytes. We unambiguously identify IAA and related metabolites in isolated Arabidopsis vacuoles, suggesting a key role for the vacuole in intracellular auxin homoeostasis. Moreover, local auxin application onto wat1 mutant stems restores fibre cell wall thickness. Our study provides new insight into the complexity of auxin transport in plants and a means to dissect auxin function during fibre differentiation. The plant hormone auxin is essential for plant development and growth and is transported across cellular membranes via specialized transporter proteins. In this study, Ranocha et al. identify the first vacuolar auxin transporter, WAT1, suggesting an involvement of the vacuole in auxin signalling.

• Publication year

  2013

• Venue

  Nature Communications

• Publication date

  2013-10-16

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1038/ncomms3625PMID 24129639PMCID 3826630
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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