Technologies, Tools, and Genetic Models to Study Osteoclasts

Abstract The osteoclast has been and still is the subject of many investgations to understand its tight regulation as a cell whose primary role is to destroy bone tissue. These large mature post-mitotic cells are difficult to isolate and manipulate since they represent a small in vivo cell population embeded in the bone tissue. This complex microenvironment is difficult to reproduce ex vivo and represent strong limitation to establish a faithful experimental cell study system. The past decades however brought tremendous progress in our capacity to analyze and understand particularly the role of the osteoclast in bone tissue maintenance. Here, we will summarize the main experimental approaches and tools that have been developed to dissect osteoclast biology.

• Publication year

  2019

• Venue

  Unknown venue

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/B978-0-12-801238-3.11169-9
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Ostm1 Bifunctional Roles in Osteoclast Maturation: Insights From a Mouse Model Mimicking a Human OSTM1 Mutation

  2018cited by this paper
• Crispr-Cas9 engineered osteogenesis imperfecta type V leads to severe skeletal deformities and perinatal lethality in mice.

  2018cited by this paper
• Transplantation of Haploidentical TcRaß‐Depleted Hematopoietic Cells Allows for Optimal Timing and Sustained Correction of the Metabolic Defect in Children With Infantile Osteopetrosis

  2017cited by this paper
• Osteoimmunology: The Conceptual Framework Unifying the Immune and Skeletal Systems.

  2017cited by this paper
• Genetic modification of ER‐Hoxb8 osteoclast precursors using CRISPR/Cas9 as a novel way to allow studies on osteoclast biology

  2017cited by this paper
• CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons.

  2017cited by this paper
• CRISPR/CAS9 Technologies

  2017cited by this paper
• Technical considerations for the use of CRISPR/Cas9 in hematology research.

  2017cited by this paper
• Generation of induced pluripotent stem cells (ARO-iPSC1-11) from a patient with autosomal recessive osteopetrosis harboring the c.212+1G>T mutation in SNX10 gene.

  2017cited by this paper
• Craniometaphyseal Dysplasia Mutations in ANKH Negatively Affect Human Induced Pluripotent Stem Cell Differentiation into Osteoclasts

  2017cited by this paper
• Open source software for semi-automated histomorphometry of bone resorption and formation parameters.

  2017cited by this paper
• In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.

  2017cited by this paper
• Pit Assay to Measure the Bone Resorptive Activity of Bone Marrow-derived Osteoclasts.

  2016cited by this paper
• Highly Efficient Genome Editing of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9.

  2016cited by this paper
• Transcriptional Modulator Ifrd1 Regulates Osteoclast Differentiation through Enhancing the NF-κB/NFATc1 Pathway

  2016cited by this paper
• Potential Pitfalls of the Mx1-Cre System: Implications for Experimental Modeling of Normal and Malignant Hematopoiesis

  2016cited by this paper
• Regulation and Function of Lentiviral Vector-Mediated TCIRG1 Expression in Osteoclasts from Patients with Infantile Malignant Osteopetrosis: Implications for Gene Therapy

  2016cited by this paper
• ATF3 controls proliferation of osteoclast precursor and bone remodeling

  2016cited by this paper
• Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells

  2016cited by this paper
• Targeted Gene Correction in Osteopetrotic-Induced Pluripotent Stem Cells for the Generation of Functional Osteoclasts

  2015cited by this paper
• Hematopoietic stem cell transplantation for infantile osteopetrosis.

  2015cited by this paper
• Regulators of G protein signaling 12 promotes osteoclastogenesis in bone remodeling and pathological bone loss

  2015cited by this paper
• Involvement of PU.1 in NFATc1 promoter function in osteoclast development.

  2015cited by this paper
• Development and applications of CRISPR-Cas9 for genome engineering.

  2014cited by this paper
• Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

  2014cited by this paper
• Osteoclasts: more than 'bone eaters'.

  2014cited by this paper
• The new frontier of genome engineering with CRISPR-Cas9

  2014cited by this paper
• The Great Beauty of the osteoclast.

  2014cited by this paper
• Runx1-mediated regulation of osteoclast differentiation and function.

  2014cited by this paper
• Vinculin Regulates Osteoclast Function*

  2014cited by this paper
• Comparative analysis of the efficiency and specificity of myeloid-Cre deleting strains using ROSA-EYFP reporter mice.

  2014cited by this paper
• Mx1‐Cre mediated Rgs12 conditional knockout mice exhibit increased bone mass phenotype

  2013cited by this paper
• Regulation of lysosome biogenesis and functions in osteoclasts

  2013cited by this paper
• Lentivirus-mediated RNA interference of DC-STAMP expression inhibits the fusion and resorptive activity of human osteoclasts

  2013cited by this paper
• Osteopetrosis: genetics, treatment and new insights into osteoclast function

  2013cited by this paper
• One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.

  2013cited by this paper
• Lentiviral gene transfer of TCIRG1 into peripheral blood CD34(+) cells restores osteoclast function in infantile malignant osteopetrosis.

  2013cited by this paper
• Transfection of osteoclasts and osteoclast precursors.

  2012cited by this paper
• RANKL-mediated osteoclast formation from murine RAW 264.7 cells.

  2012cited by this paper
• Transgenic Mice for a Tamoxifen-Induced, Conditional Expression of the Cre Recombinase in Osteoclasts

  2012cited by this paper
• Lentivirus transduction of human osteoclast precursor cells and differentiation into functional osteoclasts.

  2012cited by this paper
• The promise of induced pluripotent stem cells in research and therapy

  2012cited by this paper
• Talin1 and Rap1 Are Critical for Osteoclast Function

  2012cited by this paper
• Osteoclast stimulatory transmembrane protein and dendritic cell–specific transmembrane protein cooperatively modulate cell–cell fusion to form osteoclasts and foreign body giant cells

  2012cited by this paper
• NIH Image to ImageJ: 25 years of image analysis

  2012cited by this paper
• Akt Induces Osteoclast Differentiation through Regulating the GSK3β/NFATc1 Signaling Cascade

  2012cited by this paper
• Matrix-embedded cells control osteoclast formation

  2011cited by this paper
• Autophagy proteins regulate the secretory component of osteoclastic bone resorption.

  2011cited by this paper
• Inactivation of Glycogen Synthase Kinase-3β Is Required for Osteoclast Differentiation*

  2011cited by this paper
• A microRNA expression signature of osteoclastogenesis.

  2011cited by this paper
• Inositol polyphosphate 4-phosphatase B as a regulator of bone mass in mice and humans.

  2011cited by this paper
• Advances in osteoclast biology: old findings and new insights from mouse models

  2011cited by this paper
• Inhibition of osteoclast function reduces hematopoietic stem cell numbers in vivo.

  2011cited by this paper
• Recent advances in understanding the mechanisms of osteoclast precursor fusion

  2010cited by this paper
• Directed differentiation of hematopoietic precursors and functional osteoclasts from human ES and iPS cells.

  2010cited by this paper
• Transcriptionally active nuclei are selective in mature multinucleated osteoclasts

  2010cited by this paper
• Guidelines for assessment of bone microstructure in rodents using micro–computed tomography

  2010cited by this paper
• Osteoclast‐specific inactivation of the integrin‐linked kinase (ILK) inhibits bone resorption

  2010cited by this paper
• Sphingosine-1-phosphate mobilizes osteoclast precursors and regulates bone homeostasis

  2009cited by this paper
• Atp6v0d2 Is an Essential Component of the Osteoclast‐Specific Proton Pump That Mediates Extracellular Acidification in Bone Resorption

  2009cited by this paper
• A transgenic mouse model of inducible macrophage depletion: effects of diphtheria toxin-driven lysozyme M-specific cell lineage ablation on wound inflammatory, angiogenic, and contractive processes.

  2009cited by this paper
• Urinary bone resorption markers (deoxypyridinoline and C-terminal telopeptide of type I collagen) in healthy persons, postmenopausal osteoporosis and patients with type I diabetes.

  2009cited by this paper
• Negative Feedback Control of Osteoclast Formation through Ubiquitin-mediated Down-regulation of NFATc1*

  2009cited by this paper
• Genetics, pathogenesis and complications of osteopetrosis.

  2008cited by this paper
• Efficient and Stable Gene Expression into Human Osteoclasts Using an HIV-1–Based Lentiviral Vector

  2008cited by this paper
• OSTM1 Bone Defect Reveals an Intercellular Hematopoietic Crosstalk*

  2008cited by this paper
• NFATc1 in mice represses osteoprotegerin during osteoclastogenesis and dissociates systemic osteopenia from inflammation in cherubism.

  2008cited by this paper
• Defective Osteoclastogenesis by IKKβ-null Precursors Is a Result of Receptor Activator of NF-κB Ligand (RANKL)-induced JNK-dependent Apoptosis and Impaired Differentiation*

  2008cited by this paper
• NFATc1 induces osteoclast fusion via up-regulation of Atp6v0d2 and the dendritic cell-specific transmembrane protein (DC-STAMP).

  2008cited by this paper
• Hematopoietic stem cell-targeted neonatal gene therapy reverses lethally progressive osteopetrosis in oc/oc mice.

  2007cited by this paper
• Involvement of αvβ3 integrins in osteoclast function

  2007cited by this paper
• Estrogen Prevents Bone Loss via Estrogen Receptor α and Induction of Fas Ligand in Osteoclasts

  2007cited by this paper
• The multiple roles of osteoclasts in host defense: bone remodeling and hematopoietic stem cell mobilization.

  2007cited by this paper
• A Novel Method for Efficient Generation of Transfected Human Osteoclasts

  2007cited by this paper
• Secretion of tartrate‐resistant acid phosphatase by osteoclasts correlates with resorptive behavior

  2006cited by this paper
• Onset and Progression in Inherited ALS Determined by Motor Neurons and Microglia

  2006cited by this paper
• A Novel Receptor Activator of NF-κB (RANK) Cytoplasmic Motif Plays an Essential Role in Osteoclastogenesis by Committing Macrophages to the Osteoclast Lineage*

  2006cited by this paper
• Neonatal hematopoietic stem cell transplantation cures oc/oc mice from osteopetrosis.

  2006cited by this paper
• Making better transgenic models

  2005cited by this paper
• Unsatisfactory gene transfer into bone-resorbing osteoclasts with liposomal transfection systems

  2005cited by this paper
• Targeted expression of Cre recombinase in macrophages and osteoclasts in transgenic mice

  2005cited by this paper
• Characterization of Circulating Human Osteoclast Progenitors: Development of In Vitro Resorption Assay

  2005cited by this paper
• Clinical, genetic, and cellular analysis of 49 osteopetrotic patients: implications for diagnosis and treatment

  2005cited by this paper
• Essential Role of p38 Mitogen-activated Protein Kinase in Cathepsin K Gene Expression during Osteoclastogenesis through Association of NFATc1 and PU.1*

  2004cited by this paper
• Generation and characterization of transgenic mice that express Cre recombinase in osteoclasts

  2004cited by this paper
• Grey-lethal mutation induces severe malignant autosomal recessive osteopetrosis in mouse and human

  2003cited by this paper
• Osteoclast differentiation and activation

  2003cited by this paper
• Chloride channel 7 (CLCN7) gene mutations in intermediate autosomal recessive osteopetrosis

  2003cited by this paper
• Transgenic mice with hematopoietic and lymphoid specific expression of Cre

  2003cited by this paper
• Chloride Channel 7 (ClCN7) Gene Mutations and Autosomal Dominant Osteopetrosis, Type II

  2003cited by this paper
• U0126 and PD98059, Specific Inhibitors of MEK, Accelerate Differentiation of RAW264.7 Cells into Osteoclast-like Cells*

  2002cited by this paper
• The vacuolar (H+)-ATPases — nature's most versatile proton pumps

  2002cited by this paper
• Conditional alleles in mice: Practical considerations for tissue‐specific knockouts

  2002cited by this paper
• Highly efficient modification of bacterial artificial chromosomes (BACs) using novel shuttle vectors containing the R6Kgamma origin of replication.

  2002cited by this paper
• A Novel Zinc Finger Protein That Inhibits Osteoclastogenesis and the Function of Tumor Necrosis Factor Receptor-associated Factor 6*

  2002cited by this paper
• Loss of the ClC-7 chloride channel leads to osteopetrosis in mice and man.

  2001cited by this paper
• Albers-Schönberg disease (autosomal dominant osteopetrosis, type II) results from mutations in the ClCN7 chloride channel gene.

  2001cited by this paper
• A Glanzmann’s mutation in β3 integrin specifically impairs osteoclast function

  2001cited by this paper
• Serum CTX: A New Marker of Bone Resorption That Shows Treatment Effect More Often Than Other Markers Because of Low Coefficient of Variability and Large Changes with Bisphosphonate Therapy

  2000cited by this paper
• In Vitro and In Vivo Suppression of Osteoclast Function by Adenovirus Vector–Induced csk Gene

  2000cited by this paper

• Pickering emulsions as an effective route for the preparation of bioactive composites: A study of nanocellulose/polyaniline particles with immunomodulatory effect.

  2023cites this paper
• OSTM1 pleiotropic roles from osteopetrosis to neurodegeneration.

  2022cites this paper
• Ostm1 from Mouse to Human: Insights into Osteoclast Maturation

  2020cites this paper