Glutathione Is Recruited into the Nucleus in Early Phases of Cell Proliferation*

We have studied the possible correlation between nuclear glutathione distribution and the progression of the cell cycle. The former was studied by confocal microscopy using 5-chloromethyl fluorescein diacetate and the latter by flow cytometry and protein expression of Id2 and p107. In proliferating cells, when 41% of them were in the S+G2/M phase of the cell cycle GSH was located mainly in the nucleus. When cells reached confluence (G0/G1) GSH was localized in the cytoplasm with a perinuclear distribution. The nucleus/cytoplasm fluorescence ratio for GSH reached a maximal mean value of 4.2 ± 0.8 at 6 h after cell plating. A ratio higher than 2 was maintained during exponential cell growth. In the G0/G1 phase of the cell cycle, the nucleus/cytoplasm GSH ratio decreased to values close to 1. We report here that cells concentrate GSH in the nucleus in the early phases of cell growth, when most of the cells are in an active division phase, and that GSH redistributes uniformly between the nucleus and the cytoplasm when cells reach confluence.

• Publication year

  2007

• Venue

  Journal of Biological Chemistry

• Publication date

  2007-07-13

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.M609582200PMID 17452333
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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