Control efficiency and expressions of resistance genes in tomato plants treated with ε-poly-l-lysine against Botrytis cinerea.

The antifungal properties and the induction of resistance by ε-poly-l-lysine (ε-PL) were examined to reveal its potential in protecting tomato plants against Botrytis cinerea. As presented herein, ε-PL at 1200mg/L was found to have optimal in vitro antifungal activities, achieving an inhibition rate of 94.96%. In first-year field tests, ε-PL (1200mg/L) had a control effect of up to 79.07% against tomato grey mould. Similar results were obtained in the second year. In greenhouse experiments, ε-PL was observed to effectively reduce leaf infection, with an observed control rate at 89.22%. To define the molecular-genetic mechanisms, we compared the gene expression under four different conditions: sterile water sprayed plants (Control), Botrytis-infected plants (Inf), ε-PL-treated plants (ε-PL) and ε-PL-treated+infected plants (ε-PL+Inf). Quantitative PCR analysis at 36h after inoculation revealed that ε-PL+Inf plants exhibited significant expression and priming of several key Botrytis-induced genes in tomato. The results indicate that ε-PL promoted plant capacity of tomato to activate defense mechanisms upon pathogen attack. In total, these findings revealed that ε-PL should be an excellent biocontrol agent candidate that combined direct antifungal activity against B. cinerea and plant resistance capacity.

• Publication year

  2017

• Venue

  Pesticide Biochemistry and Physiology

• Publication date

  2017-07-29

• Fields of study

  Agricultural and Food Sciences, Medicine, Biology, Environmental Science

• Identifiers
  DOI 10.1016/j.pestbp.2017.07.007PMID 29183591
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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