Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

No abstract is available for this paper.

• Publication year

  2001

• Venue

  Methods

• Publication date

  2001-12-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1006/METH.2001.1262PMID 11846609
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• An overview of real-time quantitative PCR: applications to quantify cytokine gene expression.

  2001cited by this paper
• Quantitation of viral load using real-time amplification techniques.

  2001cited by this paper
• Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods.

  2000cited by this paper
• Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR.

  2000cited by this paper
• The transcriptional program in the response of human fibroblasts to serum.

  1999cited by this paper
• Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro.

  1999cited by this paper
• Real time quantitative PCR.

  1996cited by this paper
• Selective degradation of early-response-gene mRNAs: functional analyses of sequence features of the AU-rich elements

  1994cited by this paper
• Quantitation of thymidylate synthase, dihydrofolate reductase, and DT-diaphorase gene expression in human tumors using the polymerase chain reaction.

  1992cited by this paper
• Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction.

  1990cited by this paper
• Use of the polymerase chain reaction in the quantitation of mdr-1 gene expression.

  1990cited by this paper

• Elucidation of Response Mechanism of Potato to Nitrogen Stress by Physiological and Transcriptional Analyses

  2026cites this paper
• Deciphering the Effects of Plant Oil Diets in Channa striata (BLOCH, 1793) using an Integrated Transcriptomic and Metagenomic Approach.

  2026cites this paper
• Cellobiose induction enhances the biocontrol efficacy of Meyerozyma guilliermondii by strengthening antioxidant defense, energy metabolism and cell-wall integrity

  2026cites this paper
• The OsMYB60-OsGL1-11 module regulates drought tolerance in rice.

  2026cites this paper
• Early life stress-induced depression reveals distinct region-specific modulation of unfolded protein response genes in the prefrontal cortex and hippocampus of rats

  2026cites this paper
• Virus-host metabolic interaction: SGIV-induced protein lactylation suppresses viral propagation.

  2026cites this paper
• TMAO-Triggered Endothelial–Mesenchymal Transition and Microvesicle Release as Mediators of Vascular Smooth Muscle Cell Osteogenic Differentiation and Vascular Calcification

  2026cites this paper
• Mangifera indica (mango) ICE1s confer flowering and tolerance to multiple stresses in Arabidopsis thaliana

  2026cites this paper
• Probiotic-Driven Metabolic Transformation in Dark Tea: Enhanced Bioactives and Gut Microbiota Modulation Underlie Its Alleviation Effect Against Constipation

  2026cites this paper
• Comparative genomic analysis of the MAPKKK gene family reveals WGD-driven expansion and strong evolutionary constraints in Asteraceae

  2026cites this paper
• Bacteriocin-producing Lactococcus lactis subsp. lactis A7 enhances health status, immunity and gut microbiota in Nile tilapia (Oreochromis niloticus)

  2026cites this paper
• Boosting Fosfomycin Efficacy Against Methicillin-Resistant Staphylococcus aureus Infections by Targeting Pyrimidine Metabolism

  2026cites this paper
• Barley protein concentrate as a sustainable alternative to soybean meal in diets of hybrid African catfish (Clarias gariepinus × Heterobranchus longifilis)

  2026cites this paper
• Dose- and sex-related effects of the MAGL inhibitor MCH11 on binge-like ethanol consumption in mice.

  2026cites this paper
• β-aminobutyric acid alleviates the inhibition of production of soybean with exposure to phenanthrene stress via multilayer defense to maintain consistency

  2026cites this paper
• Specific regulation of muscle protein metabolism in broilers by dietary fermented cottonseed meal

  2026cites this paper
• Going wild in banana breeding enables Fusarium-resistant hybrids with improved fruit quality.

  2026cites this paper
• Plant-Derived Isoferulic Acid and RNAi Synergize to Overcome Detoxification Defenses in the Bird Cherry-Oat Aphid (Rhopalosiphum padi).

  2026cites this paper
• Nucleoli-localized KANSL2 as an epigenetic regulator of ribosome biogenesis in glioblastoma cells.

  2026cites this paper
• Morin Reduces Ciprofloxacin-Induced Brain Damage by Regulating Oxidative Stress, Inflammation, and Histopathological Changes

  2026cites this paper
• Loss of WT1 Drives Adaptive Plasticity in CCDC6-RET Selpercatinib-Resistant Papillary Thyroid Cancer

  2026cites this paper
• AgNPs-induced nanoelicitation: enhancing Eugenol, Rosmarinic Acid, antioxidant defense and gene expression in Ocimum tenuiflorum

  2026cites this paper
• A genome-wide study of the AP2/ERF gene family in mangrove Avicennia marina reveals the role of AmERF1 in salt tolerance through mediating proline biosynthesis and H₂O₂ homeostasis.

  2026cites this paper
• Uncovering the immune-related PCD genes in chronic rhinosinusitis with nasal polyps inflammatory progression: a machine learning and functional validation study

  2026cites this paper
• Mogroside V alleviates diquat-induced renal tubular epithelial cell injury via the TUG1/miR-29-3p/SIRT1 axis

  2026cites this paper
• Chitosan and Microalgae Nanoparticles: Synergistic Role in Enhancing Drought Stress Tolerance in Wheat Seedlings

  2026cites this paper
• From Patient Liver Tissue to Organoids: Establishment of a Translational Platform Using Healthy, Steatotic, and Cirrhotic Tissue Sources

  2026cites this paper
• Quantification Revisited: What qPCR Efficiency Models Reveal About Data Analysis Integrity

  2026cites this paper
• Ameliorative effects of desert truffle Terfezia claveryi extract on Hysterothylacium thalassini- induced oxidative damage in C57BL/6 mice

  2026cites this paper
• PdabZIP69 enhances drought tolerance by regulating antioxidant enzyme activity and PdbbHLH1 expression in "Shanxin" poplar.

  2026cites this paper
• The Underlying Influence of ARID1A Under Expression in The Pathogenesis of Colorectal Cancer

  2026cites this paper
• Combined Salt and Heat Stress Aggravates Oxidative Stress and Photosynthetic Damage, Disrupting Carbon and Nitrogen Metabolism and Yield in Rice

  2026cites this paper
• Effects of 60Co-γ irradiation on growth, photosynthetic characteristics, antioxidant systems, and micro-morphology in Platycodon grandiflorus seedlings

  2026cites this paper
• Dual-Transcriptome Dissection of the Mechanisms Underlying Alfalfa Phenotypic Differences Induced by Two Rhizobial Isolates

  2026cites this paper
• Horse immune response of recombinant Lawsonia intracellularis vaccine: Assessing the immunomodulatory impact of probiotic supplementation.

  2026cites this paper
• Identification and validation of biomarkers associated with lactic acid metabolism in diabetic nephropathy.

  2026cites this paper
• Upregulation of miR-146a-5p and miR-146b-5p limits IL-1β-mediated signaling in adipose tissue during polytrauma by downregulating IRAK1

  2026cites this paper
• Pamamycin Disrupts the Cell Envelope and Mitochondrial Potential to Inhibit Aspergillus flavus and Aflatoxin Production in a Peanut Kernel Model

  2026cites this paper
• Physiological responses and screening of key genes involved in the cold tolerance of herbaceous peony cultivated over-winter in Harbin, the coldest metropolis of China

  2026cites this paper
• Gene-Editing-Mediated Enhancement of Carotenoid Compound Accumulation in Common Wheat Grains

  2026cites this paper
• Maternal Tributyrin Supplementation During the Perinatal Period Is Associated with Improved Ewe Milk Quality and Lamb Growth Performance, Immunity, and Antioxidant Status

  2026cites this paper
• Genome-Wide Identification of Ovarian Tumor Proteases Gene Family and Knockout of TaOTU6 Increases Grain Width and Weight in Wheat

  2026cites this paper
• Effect of Fermented Buffalo Milk (Dadih) on the Expression of BACE-1 and Caspase-3 Genes in the Brain of Alzheimer Rat Model

  2026cites this paper
• Vitreous transcriptomic profiling reveals miR-4762 as a potential diagnostic biomarker and modulator of disease pathophysiology in Tubercular Uveitis.

  2026cites this paper
• Role of LSDV ORF102 in Immune Evasion and Characterization of ORF102-Deleted Recombinant Virus

  2026cites this paper
• Exploring the mechanisms by which sodium promotes growth of euhalophytes under potassium deficiency: A case study of Suaeda salsa

  2026cites this paper
• Transcriptomic insights into common defense mechanisms of Pleurotus ostreatus, Agaricus bisporus, and Lentinula edodes against Pseudomonas tolaasii-induced browning

  2026cites this paper
• Diagnostic potential of circular RNAs in carotid atherosclerotic plaque vulnerability

  2026cites this paper
• Nano-Cilostazol Mitigates Cisplatin-Induced Nephrotoxicity in Rats via Modulation of Oxidative Stress, Apoptosis, Pyroptosis, and miRNA-155 Signaling

  2026cites this paper
• Next-Generation Minimally Invasive Anti-Aging Therapy: Incorporation of Resveratrol-Nicotinamide Cerosomes into PLGA Microneedles for Enhanced Skin Permeation

  2026cites this paper
• VsAPX1 Is Up-Regulated by ABA and Heat Stress in Common Vetch (Vicia sativa)

  2026cites this paper
• Integrated Gut Microbiome and Metabolome Analysis in Largemouth Bass (Micropterus salmoides) Following Viral Infection

  2026cites this paper
• Analysis of the mechanism of MKK4 participating in heat stress response in Mytilus coruscus

  2026cites this paper
• Hepatic Effects of Etoricoxib in Mice: Integrated Histopathological and Gene Expression Analysis

  2026cites this paper
• Biochar Ameliorates Salt Stress in Maize by Modulating Physiological Responses and Expression of SOS1 and NHX2 Genes

  2026cites this paper
• Integrated genomic and transcriptomic analysis of maize SRC2-like genes highlights divergent roles in abiotic stress responses

  2026cites this paper
• Transcriptome-based identification and expression profiling of olfaction-related genes in the semi-aquatic firefly Pygoluciola qingyu (Coleoptera: Lampyridae)

  2026cites this paper
• Identification of candidate loci regulating seed-associated traits in soybean using genome-wide association study and image-based high-throughput phenotyping

  2026cites this paper
• Sorghum BRS 305 bran phenolic extract modulates the intestinal inflammation, morphology, and ileum–brain satiety pathway in vivo (Gallus gallus)

  2026cites this paper
• Environmental-Nitrite-Enhanced Cyprinid Herpesvirus 2 Infection in Crucian Carp

  2026cites this paper
• tca-miR-137-5p modulates phosphine resistance by targeting CYP346B3 in Tribolium castaneum

  2026cites this paper
• MdHMGB15 enhances zinc tolerance by activating multiple metal homeostasis genes in apple.

  2026cites this paper
• Thermal desiccation and expression of HSP90 and osmotic avoidance protein genes in an acorn barnacle, Amphibalanus amphitrite.

  2026cites this paper
• Attenuating effect of plumbagin on Chromobacterium violaceum quorum sensing and biofilm formation: an in-vitro and in-silico approach.

  2026cites this paper
• Mast cell desensitization induces a distinct IgE-dependent transcriptional program associated with immune regulation

  2026cites this paper
• Peroxisomal acyl-CoA oxidase OsACX4 negatively regulates salt and drought stress tolerance by modulating cellular redox homeostasis in rice.

  2026cites this paper
• Sex-dependent dysregulation of the gut-brain NPYergic system in a mouse model of autism spectrum disorder.

  2026cites this paper
• Genome-wide identification of castor bean OSCA genes and analysis of expression patterns under drought and salt stress.

  2026cites this paper
• Global profiling of nascent chain interactors reveals TRIM25 as a co-translational E3 ubiquitin ligase.

  2026cites this paper
• Reporting quality of quantitative polymerase chain reaction (qPCR) methods in scientific publications.

  2026cites this paper
• cIAP1 inhibitor of apoptosis is a tumor suppressor in Ewing sarcoma.

  2026cites this paper
• Mitigation of Fomesafen Phytotoxicity in Sugar Beet by Biochar through Modulation of Antioxidant Metabolism and Photosynthetic Gene Regulation.

  2026cites this paper
• Phenotypic fate differentiation and its mechanism in Cronobacter sakazakii under different chlorine concentrations.

  2026cites this paper
• Mechanism of co-transcriptional cap snatching by influenza polymerase.

  2026cites this paper
• Functional Characterization of Interferon-Induced Protein 44-Like in Amphiprion clarkii: Expression Profiles, Immunomodulatory Activity, and Antiviral Function.

  2026cites this paper
• Phosphodiesterase 4 (PDE 4) Inhibition Reduces Ischemia–Reperfusion-Induced Leucocyte Infiltration, Apoptosis and Mitochondrial Fission Markers in Mice Skeletal Muscles Four Hours After Ischemia Onset

  2026cites this paper
• Molecular role of DRG1 in coordinating osteoclast and osteoblast differentiation during bone remodeling.

  2026cites this paper
• Pro-Inflammatory Response of Bovine Lung Explant Induced by Mycoplasma mycoides subsp. mycoides

  2026cites this paper
• Thiamine ameliorates subacute ruminal acidosis-induced mastitis in goats and is associated with modulation of the NF-κB/NLRP3/CLOCK axis and rumen microbial homeostasis.

  2026cites this paper
• Protective effects of morin on colistin-induced neurotoxicity in rats via modulation of oxidative, inflammatory, apoptotic, and autophagic alterations.

  2026cites this paper
• The conserved long noncoding RNA LTCONS12135 positively regulates innate immunity in teleost.

  2026cites this paper
• Nano-enabled enhancement of salt stress tolerance in barley using chitosan-selenium nanoparticles: physiological and molecular insights.

  2026cites this paper
• Type VI secretion system degeneration accelerates intestinal epithelial cell death in Escherichia coli O157:H7.

  2026cites this paper
• Unraveling the oncogenic and immunomodulatory roles of GINS1: a systematic pan-cancer study.

  2026cites this paper
• Integrative genomic, phylogenetic, and transcriptomic analyses reveal temperature-responsive transient receptor potential (TRP) genes in Pacific abalone Haliotis discus hannai.

  2026cites this paper
• Exploratory study on the impact of Ganoderma australe extract on gut microbiota and immune gene expression in honey bees exposed to Vairimorpha ceranae.

  2026cites this paper
• Decoding Arabidopsis growth-defense trade-offs through ADR1-associated transcriptional networks.

  2026cites this paper
• Elucidating the Ameliorative Roles of Dietary Organic Selenium on the Mitigation of Exposure to Cadmium Stress in Stripped Mullet (Mugil cephalus): Exploring Growth Rate, Stress Biomarkers, Immune and Reproduction-Related Genes.

  2026cites this paper
• HDAC7 aggravates malignant proliferation of hepatocellular carcinoma cells via the TRIM26/CBX4 axis

  2026cites this paper
• Effects of alfalfa as a substitute for dietary kelp on the growth, appetite, digestion, intestine histology, and immunity of abalone Haliotis discus hannai

  2026cites this paper
• Comparative cytology and transcriptome analysis revealed that PpMED15A drives the fertility of peach pollen

  2026cites this paper
• Identification of TgENT1 as the TgUUT1 Uracil/Uridine Transporter of Toxoplasma gondii

  2026cites this paper
• Promotion of growth and biochemical changes in the maize seedlings after pre-sowing treatment of seeds with oxygen plasma

  2026cites this paper
• WD40 Gene Family in Paulownia: Genome-Wide Identification, Stress Regulation, and Pathogen Effector Interactions.

  2026cites this paper
• Structure, evolution, phylogeny, and analysis of domain-deficient genes in the IQD gene family of Brassica juncea.

  2026cites this paper
• АНАЛИЗ КОПИЙНОСТИ РЕТРОТРАНСПОЗОНА ONSEN В ПОПУЛЯЦИИ A. THALIANA ПОСЛЕ ПРИМЕНЕНИЯ КОМБИНИРОВАННОГО СТРЕССА

  2025cites this paper
• Improvement of fertility in repeat breeder dairy cattle by embryo transfer following artificial insemination: possibility of interferon tau replenishment effect

  2019cites this paper
• Behavioral Neuroscience

  year unknowncites this paper
• Dynamic expression of Slit1-3 and Robo1-2 in the mouse Dynamic expression of Slit1-3 and Robo1-2 in the mouse peripheral nervous system after injury peripheral nervous system after injury

  year unknowncites this paper
• Anais Brasileiros de Dermatologia

  year unknowncites this paper