Carbon Starved Anther Encodes a MYB Domain Protein That Regulates Sugar Partitioning Required for Rice Pollen Development[W][OA]

The authors identified a rice R2R3 MYB transcription factor, Carbon Starved Anther (CSA), that regulates sugar partitioning from leaves to anthers and is required for the production of functional pollen. CSA directly regulates the expression of the monosaccharide transporter gene MST8, which encodes a key component of the anther sugar unloading pathway. In flowering plants, sink tissues rely on transport of carbohydrates from photosynthetic tissues (sources) for nutrition and energy. However, how sugar partitioning in plants is regulated at the molecular level during development remains unknown. We have isolated and characterized a rice (Oryza sativa) mutant, carbon starved anther (csa), that showed increased sugar contents in leaves and stems and reduced levels of sugars and starch in floral organs. In particular, the csa mutant had reduced levels of carbohydrates in later anthers and was male sterile. The csa mutant had reduced accumulation of 14C-labeled sugars in anther sink tissue. CSA was isolated by map-based cloning and was shown to encode an R2R3 MYB transcription factor that was expressed preferentially in the anther tapetal cells and in the sugar-transporting vascular tissues. In addition, the expression of MST8, encoding a monosaccharide transporter, was greatly reduced in csa anthers. Furthermore, CSA was found to be associated in vivo and in vitro with the promoter of MST8. Our findings suggest that CSA is a key transcriptional regulator for sugar partitioning in rice during male reproductive development. This study also establishes a molecular model system for further elucidation of the genetic control of carbon partitioning in plants.

• Publication year

  2010

• Venue

  The Plant Cell

• Publication date

  2010-03-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1105/tpc.109.073668PMID 20305120PMCID PMC2861464
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2009cited by this paper
• Cell Wall-Bound Invertase Limits Sucrose Export and Is Involved in Symptom Development and Inhibition of Photosynthesis during Compatible Interaction between Tomato and Xanthomonas campestris pv vesicatoria[W][OA]

  2008influential reference
• Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.)

  2008cited by this paper
• Control of rice grain-filling and yield by a gene with a potential signature of domestication

  2008cited by this paper
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  2008cited by this paper
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  2007cited by this paper
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  2007cited by this paper
• Virus-induced gene silencing as a tool for functional analyses in the emerging model plant Aquilegia (columbine, Ranunculaceae)

  2007cited by this paper
• Rice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male Sterility[W][OA]

  2007cited by this paper
• ABA regulates apoplastic sugar transport and is a potential signal for cold-induced pollen sterility in rice.

  2007influential reference
• The monosaccharide transporter(‐like) gene family in Arabidopsis

  2007influential reference
• Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization

  2007cited by this paper
• Pollen carbohydrates and water content during development, presentation, and dispersal: a short review

  2006cited by this paper
• The Rice Tapetum Degeneration Retardation Gene Is Required for Tapetum Degradation and Anther Development[W]

  2006cited by this paper
• Expression and purification of the synthetic preS1 gene of Hepatitis B Virus with preferred Escherichia coli codon preference.

  2006cited by this paper
• Sugar sensing and signaling in plants: conserved and novel mechanisms.

  2006cited by this paper
• Isolation and Genetic Analysis for Rice Mutants Treated with ~(60)Co γ-Ray

  2006cited by this paper
• Gas chromatography mass spectrometry–based metabolite profiling in plants

  2006cited by this paper
• Effects of chilling on male gametophyte development in rice

  2006cited by this paper
• The MYB Transcription Factor Superfamily of Arabidopsis: Expression Analysis and Phylogenetic Comparison with the Rice MYB Family

  2005cited by this paper
• Expression profiling of genes involved in starch synthesis in sink and source organs of rice.

  2005influential reference
• Cold-induced repression of the rice anther-specific cell wall invertase gene OSINV4 is correlated with sucrose accumulation and pollen sterility

  2005influential reference
• AtSTP11, a pollen tube-specific monosaccharide transporter in Arabidopsis

  2005cited by this paper
• Molecular genetic analyses of microsporogenesis and microgametogenesis in flowering plants.

  2005cited by this paper
• Control of Male Gametophyte Development

  2004cited by this paper
• Stamen Structure and Function

  2004cited by this paper
• Loss-of-function mutations of the rice GAMYB gene impair alpha-amylase expression in aleurone and flower development.

  2004cited by this paper
• The ANTHER INDEHISCENCE1 Gene Encoding a Single MYB Domain Protein Is Involved in Anther Development in Rice1

  2004cited by this paper
• Cloning and identification of highly expressed genes in barley lemma and palea

  2004cited by this paper
• MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment

  2004influential reference
• Transport mechanisms for organic forms of carbon and nitrogen between source and sink.

  2004cited by this paper
• The sucrose transporter gene family in rice.

  2003cited by this paper
• Characterization of Rice Functional Monosaccharide Transporter, OsMST5

  2003cited by this paper
• Roles of cell-wall invertases and monosaccharide transporters in the growth and development of Arabidopsis.

  2003cited by this paper
• Production of FaeG, the Major Subunit of K88 Fimbriae, in Transgenic Tobacco Plants and Its Immunogenicity in Mice

  2003cited by this paper
• AtSTP6, a New Pollen-Specific H+-Monosaccharide Symporter from Arabidopsis1

  2003cited by this paper
• Functional Characterization and Expression Analyses of the Glucose-Specific AtSTP9 Monosaccharide Transporter in Pollen of Arabidopsis1

  2003cited by this paper
• AtSTP 6 , a New Pollen-Specific H-Monosaccharide Symporter from Arabidopsis 1

  2003cited by this paper
• MrBayes 3: Bayesian phylogenetic inference under mixed models

  2003cited by this paper
• PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences

  2002cited by this paper
• A chromatin immunoprecipitation (ChIP) approach to isolate genes regulated by AGL15, a MADS domain protein that preferentially accumulates in embryos.

  2002cited by this paper
• Three Novel MYB Proteins with One DNA Binding Repeat Mediate Sugar and Hormone Regulation of α-Amylase Gene Expression Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.001735.

  2002cited by this paper
• Starch Biosynthesis during Pollen Maturation Is Associated with Altered Patterns of Gene Expression in Maize1

  2002cited by this paper
• [A simple and rapid methods used for silver staining and gel preservation].

  2002cited by this paper
• Induction of male sterility in plants by metabolic engineering of the carbohydrate supply

  2001cited by this paper
• MRBAYES: Bayesian inference of phylogenetic trees

  2001cited by this paper
• Characterization of sucrose uptake system in cassava (Manihot esculenta Crantz).

  2001cited by this paper
• Molecular phylogenetics: state-of-the-art methods for looking into the past.

  2001cited by this paper
• Pollen Development and Its Stages in Rice (Oryza sativa L.)

  2001cited by this paper
• Characterization and expression of monosaccharide transporters (osMSTs) in rice.

  2000cited by this paper
• Genetic evidence for the in planta role of phloem-specific plasma membrane sucrose transporters.

  2000cited by this paper
• Plant Nuclei Can Contain Extensive Grooves and Invaginations

  2000cited by this paper
• Sucrose transporters in plants: update on function and structure.

  2000cited by this paper
• A male gametophyte-specific monosaccharide transporter in Arabidopsis.

  1999cited by this paper
• Sucrose-cleaving enzymes and carbohydrate pools in Lilium longiflorum floral organs

  1998influential reference
• Transformation of rice mediated by Agrobacterium tumefaciens

  1997cited by this paper
• The Mechanics of the Grass Flower: Anther Dehiscence and Pollen Shedding in Maize

  1996cited by this paper
• Anther wall layers control pollen sugar nutrition inLilium

  1995influential reference
• The cellular pathway of postphloem sugar transport in developing tomato fruit

  1995cited by this paper
• Isolation and characterization of novel nodulin cDNAs representing genes expressed at early stages of soybean nodule development

  1993cited by this paper
• REGULATION OF ALPHA-AMYLASE GENE EXPRESSION

  1986cited by this paper
• [C]Sucrose Uptake and Labeling of Starch in Developing Grains of Normal and segl Barley.

  1984influential reference

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  2026cites this paper
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  2026cites this paper
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  2026cites this paper
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  2025cites this paper
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• Advances in plant male sterility for hybrid seed production: an overview of conditional nuclear male sterile lines and biotechnology-based male sterile systems

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  2024cites this paper
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  2024cites this paper
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  2024cites this paper
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  2024cites this paper
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  2023cites this paper
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  2023cites this paper
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  2023cites this paper
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  2023cites this paper
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  2023cites this paper
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  2023cites this paper
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  2023cites this paper
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  2023cites this paper
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  2022cites this paper
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  2022cites this paper
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  2022cites this paper
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  2022cites this paper
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  2022cites this paper
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  2022cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper