Fibronectin attachment activates the NF-kappa B p50/p65 heterodimer in fibroblasts and smooth muscle cells.

The nuclear factor kappa B, NF-kappa B, is activated by numerous inflammatory mediators, regulating biological responses that in part are dependent on the extracellular matrix. Analyses of the influence of cell-matrix interaction on NF-kappa B activation revealed that attachment to fibronectin caused an increase in NF-kappa B activity corresponding to 3-fold the level in cells plated on bare plastic. This effect was dependent on the degree of attachment and appeared to involve both the RGD motif of the cell-binding domain, and the heparin-binding domain of fibronectin. Fibronectin attachment specifically caused activation of the p50/p65 heterodimeric form of NF-kappa B, resulting in an increase of this complex to a level similar to that induced by cytokine stimulation. These data show that cell attachment has a pronounced influence on the level of NF-kappa B activity, affecting specific dimeric complexes, and suggest this to be a pathway through which effects depending on cell-matrix interaction can regulate cell behavior and cytokine responses.

• Publication year

  1994

• Venue

  Journal of Biological Chemistry

• Publication date

  1994-12-09

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(18)47345-0PMID 7983003
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The extracellular matrix ligands fibronectin and tenascin collaborate in regulating collagenase gene expression in fibroblasts.

  1994cited by this paper
• Stimulation of transcription factors NF kappa B and AP1 in endothelial cells subjected to shear stress.

  1994cited by this paper
• Bcl-3-mediated nuclear regulation of the NF-kappa B trans-activating factor.

  1994cited by this paper
• Signal transduction from the extracellular matrix

  1993cited by this paper
• Regulation of the NF-kappa B/rel transcription factor and I kappa B inhibitor system.

  1993cited by this paper
• A novel NF-kappa B complex containing p65 homodimers: implications for transcriptional control at the level of subunit dimerization

  1993cited by this paper
• The inducible transcription factor NF-kappa B: structure-function relationship of its protein subunits.

  1993cited by this paper
• pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.

  1992cited by this paper
• NF-kappa B and related proteins: Rel/dorsal homologies meet ankyrin-like repeats.

  1992cited by this paper
• Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly

  1992cited by this paper
• The small GTP-binding protein rac regulates growth factor-induced membrane ruffling.

  1992cited by this paper
• The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors.

  1992cited by this paper
• Interleukin 1 beta induces rapid phosphorylation and redistribution of talin: a possible mechanism for modulation of fibroblast focal adhesion.

  1991cited by this paper
• Interleukin-1 represents a new modality for the activation of extracellular signal-regulated kinases/microtubule-associated protein-2 kinases.

  1991cited by this paper
• Cytokines in context

  1991cited by this paper
• Evidence that interleukin-1 and phorbol esters activate NF-kappa B by different pathways: role of protein kinase C.

  1991cited by this paper
• Signal transduction by integrins: increased protein tyrosine phosphorylation caused by clustering of beta 1 integrins.

  1991cited by this paper
• Transmembrane molecular assemblies in cell-extracellular matrix interactions.

  1991cited by this paper
• Role of cAMP in interleukin-1-induced kappa light chain gene expression in murine B cell line.

  1990cited by this paper
• Fibronectin receptor overexpression and loss of transformed phenotype in a stable variant of the K562 cell line.

  1990cited by this paper
• Analysis of cDNA for human erythrocyte ankyrin indicates a repeated structure with homology to tissue-differentiation and cell-cycle control proteins

  1990cited by this paper
• Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression

  1989cited by this paper
• Binding, internalization, and intracellular localization of interleukin-1 beta in human diploid fibroblasts.

  1988cited by this paper
• Integrins: a family of cell surface receptors.

  1987cited by this paper
• Multiple nuclear factors interact with the immunoglobulin enhancer sequences.

  1986cited by this paper
• Selective inhibition of fibronectin-mediated cell adhesion by monoclonal antibodies to a cell-surface glycoprotein.

  1985cited by this paper
• Recent advances in research on fibronectin and other cell attachment proteins

  1985cited by this paper
• Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule

  1984cited by this paper
• Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

  1983cited by this paper
• Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule.

  1981cited by this paper

• Intermittent hypoxia-reoxygenation-induced miRNAs inhibit expression of IRF and interferon genes but activate NF-κB and expression of pulmonary fibrosis markers in human small airway epithelial cells.

  2025cites this paper
• Inhibitory immune checkpoints suppress the surveillance of senescent cells promoting their accumulation with aging and in age-related diseases

  2024cites this paper
• Cyclic-AMP Increases Nuclear Actin Monomer Which Promotes Proteasomal Degradation of RelA/p65 Leading to Anti-Inflammatory Effects

  2022cites this paper
• Molecular Regulation of Sprouting Angiogenesis.

  2017cites this paper
• Nonenzymatic glycation interferes with fibronectin‐integrin interactions in vascular smooth muscle cells

  2017cites this paper
• A loop of cancer-stroma-cancer interaction promotes peritoneal metastasis of ovarian cancer via TNFα-TGFα-EGFR

  2017cites this paper
• Receptor activator of NF-κB ligand induces cell adhesion and integrin α2 expression via NF-κB in head and neck cancers

  2016cites this paper
• Mutually Exclusive Roles of SHARPIN in Integrin Inactivation and NF-κB Signaling

  2015cites this paper
• Vascular smooth muscle cell in atherosclerosis

  2015cites this paper
• Anti-Inflammatory Activity of Ezetimibe by Regulating NF-κB/MAPK Pathway in THP-1 Macrophages

  2014cites this paper
• Mechanisms Regulating Smooth Muscle Cell Phenotype by Atheroprone and Atheroprotective Hemodynamics on Endothelial Cells Doctor of Philosophy

  2013cites this paper
• Extracellular Matrix and Smooth Muscle Cells

  2012cites this paper
• RANKL expression specifically observed in vivo promotes epithelial mesenchymal transition and tumor progression.

  2011cites this paper
• Chlorogenic acid attenuates adhesion molecules upregulation in IL-1β-treated endothelial cells

  2010cites this paper
• NIK and IKKbeta interdependence in NF-kappaB signalling - Flux analysis of regulation through metabolites

  2010cites this paper
• IKK interdependence in NF-B signalling — Flux analysis of regulation hrough metabolites

  2010cites this paper
• Entamoeba histolytica Cysteine Proteinase 5 Binds Integrin on Colonic Cells and Stimulates NFκB-mediated Pro-inflammatory Responses*

  2010cites this paper
• Complex Regulation and Function of the Inflammatory Smooth Muscle Cell Phenotype in Atherosclerosis

  2009cites this paper
• Interleukin‐18 stimulates fibronectin expression in primary human cardiac fibroblasts via PI3K‐Akt‐dependent NF‐κB activation

  2008cites this paper
• Activation of the NF-KB and IK B System in Smooth Muscle Cells after Rat Arterial Injury Induction of Vascular Cell Adhesion Molecule- 1 and Monocyte Chemoattractant Protein- 1

  2007cites this paper
• Protective effect of hyaluronic acid on interleukin-1-induced deregulation of β1-integrin and insulin-like growth factor-I receptor signaling and collagen biosynthesis in cultured human chondrocytes

  2007cites this paper
• Ellagic acid inhibits IL-1beta-induced cell adhesion molecule expression in human umbilical vein endothelial cells.

  2007cites this paper
• Epithelial-derived Fibronectin Expression, Signaling, and Function in Intestinal Inflammation*

  2007cites this paper
• Extra‐cellular matrix proteins induce re‐distribution of α‐actinin‐1 and α‐actinin‐4 in A431 cells

  2007cites this paper
• Extra-cellular matrix proteins induce re-distribution of alpha-actinin-1 and alpha-actinin-4 in A431 cells.

  2007cites this paper
• Mast cell chymase induces smooth muscle cell apoptosis by disrupting NF-kappaB-mediated survival signaling.

  2006cites this paper
• Prolidase-independent mechanism of camptothecin-induced inhibition of collagen biosynthesis in cultured human skin fibroblasts.

  2006cites this paper
• Src kinase activity is required for integrin alphaVbeta3-mediated activation of nuclear factor-kappaB.

  2005cites this paper
• Involvement of c-Jun NH2 Terminal Kinase and p38MAPK in Rapamycin-Mediated Inhibition of Neointimal Formation in Rat Carotid Arteries

  2005cites this paper
• Extracellular Matrix-Induced Signaling Pathways And Pancreatic Beta Cell Survival

  2005cites this paper
• Extracellular matrix-induced signaling pathways and pancreatic beta cell survival

  2005cites this paper
• Activation of NF-κB by Extracellular Matrix Is Involved in Spreading and Glucose-stimulated Insulin Secretion of Pancreatic Beta Cells*

  2005cites this paper
• Src Kinase Activity Is Required for Integrin αVβ3-Mediated Activation of Nuclear Factor-κB*

  2005cites this paper
• Activation of NF-kappaB by extracellular matrix is involved in spreading and glucose-stimulated insulin secretion of pancreatic beta cells.

  2005cites this paper
• A Bit-role for integrins in apoptosis

  2004cites this paper
• α3β1 integrin induced suppression of the Caco-2 epithelial cell IL-1 signaling pathway leading to NF-κB activation

  2004cites this paper
• Fibronectin suppresses apoptosis and protects mice from endotoxic shock.

  2004cites this paper
• alpha3beta1 integrin induced suppression of the Caco-2 epithelial cell IL-1 signaling pathway leading to NF-(kappa)B activation.

  2004cites this paper
• Regulation of nuclear translocation of nuclear factor-kappaB relA: evidence for complex dynamics at the single-cell level.

  2003cites this paper
• Activation of NF‐κB transcription factor in human neutrophils by sulphatides and L‐selectin cross‐linking

  2003cites this paper
• An NF-kappaB-dependent transcriptional program is required for collagen remodeling by human smooth muscle cells.

  2003cites this paper
• RelA Control of IκBα Phosphorylation

  2003cites this paper
• Molecular and Functional Interdependence of the Urokinase-Type Plasminogen Activator System with Integrins

  2003cites this paper
• Darstellung von aktiviertem NF-κB in der arteriosklerotischen Läsion

  2003cites this paper
• Role of nuclear factor-kappa B in the regulation of intercellular adhesion molecule 1 after infection of human bronchial epithelial cells by Bordetella pertussis.

  2003cites this paper
• An NF-κB-dependent Transcriptional Program Is Required for Collagen Remodeling by Human Smooth Muscle Cells*

  2003cites this paper
• RelA control of IkappaBalpha phosphorylation: a positive feedback loop for high affinity NF-kappaB complexes.

  2003cites this paper
• α5β1 Integrin Activates an NF-κB-Dependent Program of Gene Expression Important for Angiogenesis and Inflammation

  2002cites this paper
• Perturbation of beta1-integrin function in involuting mammary gland results in premature dedifferentiation of secretory epithelial cells.

  2002cites this paper
• Oxidative stress and peritoneal endometriosis.

  2002cites this paper
• NF-kappaB activates fibronectin gene expression in rat hepatocytes.

  2002cites this paper
• PDGF Enhancement of IL-1 Receptor Levels in Smooth Muscle Cells Involves Induction of an Attachment-Regulated, Heparan Sulfate Binding Site (IL-1RIII)

  2002cites this paper
• Alpha 5 beta 1 integrin activates an NF-kappa B-dependent program of gene expression important for angiogenesis and inflammation.

  2002cites this paper
• NF-κB as a Therapeutic Target for Cardiovascular Disease

  2002cites this paper
• NF-κB activates fibronectin gene expression in rat hepatocytes

  2002cites this paper
• Disruption of the actin cytoskeleton regulates cytokine-induced iNOS expression.

  2001cites this paper
• Fibronectin regulates the activation of THP-1 cells by TGF-β1

  2001cites this paper
• Fibronectin regulates the activation of THP-1 cells by TGF-beta1.

  2001cites this paper
• Degradation of IkappaBalpha is limited by a postphosphorylation/ubiquitination event.

  2001cites this paper
• Ras controls tumor necrosis factor receptor-associated factor (TRAF)6-dependent induction of nuclear factor-kappa b. Selective regulation through receptor signaling components.

  2001cites this paper
• Integrin αvβ3/Vitronectin Interaction Affects Expression of the Urokinase System in Human Ovarian Cancer Cells*

  2001cites this paper
• TRANSLOCATION OF THE IL‐1 RECEPTOR TO FOCAL ADHESIONS IS REGULATED THROUGH THE C‐TERMINAL END OF THE CYTOPLASMIC DOMAIN

  2001cites this paper
• Degradation of IκBα Is Limited by a Postphosphorylation/Ubiquitination Event☆

  2001cites this paper
• Involvement of Nuclear Factor-κB in Endothelin-A-Receptor–Induced Proliferation and Inhibition of Apoptosis

  2001cites this paper
• The p65/RelA Subunit of NF-κB Interacts with Actin-Containing Structures

  2000cites this paper
• The p65/RelA subunit of NF-kappaB interacts with actin-containing structures.

  2000cites this paper
• Pyrrolidine dithiocarbamate-induced apoptosis depends on cell type, density, and the presence of Cu(2+) and Zn(2+).

  2000cites this paper
• Global expression analysis of extracellular matrix-integrin interactions in monocytes.

  2000cites this paper
• The IL-1 receptor and Rho directly associate to drive cell activation in inflammation.

  1999cites this paper
• PTEN gene and integrin signaling in cancer.

  1999cites this paper
• REVIEW PTEN Gene and Integrin Signaling in Cancer

  1999cites this paper
• Respiratory epithelial cells release interleukin-8 in response to a thermophilic bacteria that causes hypersensitivity pneumonitis.

  1999cites this paper
• Activation of nuclear factor kappaB in single living cells. Dependence of nuclear translocation and anti-apoptotic function on EGFPRELA concentration.

  1999cites this paper
• Regulation of the p75 Neurotrophin Receptor in a Rat Myogenic Cell Line (L6)

  1999cites this paper
• Apoptosis overrides survival signals through a caspase-mediated dominant-negative NF-κB loop

  1999cites this paper
• Recruitment of a Heparan Sulfate Subunit to the Interleukin-1 Receptor Complex

  1999cites this paper
• Fibronectin Binding Promotes a PKC-Dependent Modulation of NF-κB in Human T Cells

  1998cites this paper
• Involvement of calcium in retinal pigment epithelial cell proliferation and pigmentation.

  1998cites this paper
• Fibronectin binding promotes a PKC-dependent modulation of NF-kappa B in human T cells.

  1998cites this paper
• Propensity for macrophage apoptosis is related to the pattern of expression and function of integrin extracellular matrix receptors.

  1998cites this paper
• Regulation of interleukin 1 signalling through integrin binding and actin reorganization: disparate effects on NF-kappaB and stress kinase pathways.

  1998cites this paper
• Activator protein-1 is the preferred transcription factor for cooperative interaction with nuclear factor-kappaB in respiratory syncytial virus-induced interleukin-8 gene expression in airway epithelium.

  1998cites this paper
• Cell Cycle and Cell Division

  1998cites this paper
• The NF-κB and IκB System in Injured Arteries

  1998cites this paper
• Globoside-dependent Adhesion of Human Embryonal Carcinoma Cells, Based on Carbohydrate-Carbohydrate Interaction, Initiates Signal Transduction and Induces Enhanced Activity of Transcription Factors AP1 and CREB*

  1998cites this paper
• NF-κB Mediates αvβ3 Integrin-induced Endothelial Cell Survival

  1998cites this paper
• A Three-dimensional Collagen Lattice Activates NF-κB in Human Fibroblasts: Role in Integrin α2 Gene Expression and Tissue Remodeling

  1998cites this paper
• Transcription factor NF-kappaB regulation of renal fibrosis during ureteral obstruction.

  1998cites this paper
• NF-k B Mediates a v b 3 Integrin-induced Endothelial Cell Survival

  1998cites this paper
• The 80‐kD fibronectin fragment increases the production of fibronectin and tumour necrosis factor‐alpha (TNF‐α) in cultured mesangial cells

  1997cites this paper
• Role of nuclear factor‐kappa B in atherogenesis

  1997cites this paper
• Activation of the NF-kappa B and I kappa B system in smooth muscle cells after rat arterial injury. Induction of vascular cell adhesion molecule-1 and monocyte chemoattractant protein-1.

  1997cites this paper
• Cell-matrix interactions in the glomerular mesangium.

  1996cites this paper
• Differentiated properties and proliferation of arterial smooth muscle cells in culture.

  1996cites this paper
• NF-kappa B is induced in the nuclei of cultured rat aortic smooth muscle cells by stimulation of various growth factors.

  1996cites this paper
• Fibrillar collagen inhibits arterial smooth muscle proliferation through regulation of Cdk2 inhibitors.

  1996cites this paper
• Activated transcription factor nuclear factor-kappa B is present in the atherosclerotic lesion.

  1996cites this paper
• Induction of interleukin (IL)-8 gene expression by respiratory syncytial virus involves activation of nuclear factor (NF)-kappa B and NF-IL-6.

  1996cites this paper
• Multiple mechanisms may contribute to the cellular anti-adhesive effects of phosphorothioate oligodeoxynucleotides.

  1996cites this paper
• The regulation of growth and intracellular signaling by integrins.

  1996cites this paper