A KDEL-tagged monoclonal antibody is efficiently retained in the endoplasmic reticulum in leaves, but is both partially secreted and sorted to protein storage vacuoles in seeds.

No abstract is available for this paper.

• Publication year

  2006

• Venue

  Plant Biotechnology Journal

• Publication date

  2006-09-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1111/J.1467-7652.2006.00200.XPMID 17309727
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Functional Analysis

  2023cited by this paper
• Glycosylation of Recombinant Antibody Therapeutics

  2008cited by this paper
• Immunoglobulin G specifically binding plant N-glycans with high affinity could be generated in rabbits but not in mice.

  2006cited by this paper
• Biopharmaceutical production in plants: problems, solutions and opportunities.

  2005cited by this paper
• Antibody processing and engineering in plants, and new strategies for vaccine production.

  2005cited by this paper
• Protein modifications in the plant secretory pathway: current status and practical implications in molecular pharming.

  2005cited by this paper
• Plant-derived mouse IgG monoclonal antibody fused to KDEL endoplasmic reticulum-retention signal is N-glycosylated homogeneously throughout the plant with mostly high-mannose-type N-glycans.

  2005cited by this paper
• Inhibition of tumor growth by plant-derived mAb.

  2005cited by this paper
• Sorting of proteins to storage vacuoles: how many mechanisms?

  2005cited by this paper
• Sowing the seeds of success: pharmaceutical proteins from plants.

  2005cited by this paper
• Specificity of IgG and IgE antibodies against plant and insect glycoprotein glycans determined with artificial glycoforms of human transferrin.

  2004cited by this paper
• Unexpected Deposition Patterns of Recombinant Proteins in Post-Endoplasmic Reticulum Compartments of Wheat Endosperm1

  2004influential reference
• Endoplasmic reticulum-resident proteins are constitutively transported to vacuoles for degradation.

  2004cited by this paper
• A recombinant multimeric immunoglobulin expressed in rice shows assembly-dependent subcellular localization in endosperm cells.

  2004cited by this paper
• Glycoprotein sialylation in plants?

  2004cited by this paper
• Degradation of transport-competent destabilized phaseolin with a signal for retention in the endoplasmic reticulum occurs in the vacuole

  2004cited by this paper
• Production and glycosylation of plant-made pharmaceuticals: the antibodies as a challenge.

  2004cited by this paper
• Molecular mechanism of enantioselective proton transfer to carbon in catalytic antibody 14D9.

  2004cited by this paper
• Where, oh where has my protein gone?

  2004cited by this paper
• Recombinant anti-hCG antibodies retained in the endoplasmic reticulum of transformed plants lack core-xylose and core-alpha(1,3)-fucose residues.

  2004cited by this paper
• Localization of a bacterial protein in starch granules of transgenic maize kernels

  2003cited by this paper
• Expression and localization of human lysozyme in the endosperm of transgenic rice

  2003cited by this paper
• 'Molecular farming' of antibodies in plants

  2003cited by this paper
• Plant cell factories and mucosal vaccines

  2003cited by this paper
• Immunotherapy: past, present and future

  2003cited by this paper
• Genetic modification: The production of recombinant pharmaceutical proteins in plants

  2003cited by this paper
• Freeze‐substitution protocols for improved visualization of membranes in high‐pressure frozen samples

  2003cited by this paper
• C-Terminal KDEL Sequence of A KDEL-Tailed Cysteine Proteinase (Sulfhydryl-Endopeptidase) Is Involved in Formation of KDEL Vesicle and in Efficient Vacuolar Transport of Sulfhydryl-Endopeptidase1

  2003cited by this paper
• Function and glycosylation of plant-derived antiviral monoclonal antibody

  2003cited by this paper
• Production of a functional catalytic antibody ScFv-NusA fusion protein in bacterial cytoplasm.

  2003cited by this paper
• Immunoreactivity in mammals of two typical plant glyco-epitopes, core alpha(1,3)-fucose and core xylose.

  2003cited by this paper
• Boosting heterologous protein production in transgenic dicotyledonous seeds using Phaseolus vulgaris regulatory sequences

  2002cited by this paper
• Multivesicular bodies: a mechanism to package lytic and storage functions in one organelle?

  2002cited by this paper
• Developing Seeds of Arabidopsis Store Different Minerals in Two Types of Vacuoles and in the Endoplasmic Reticulum Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010486.

  2002cited by this paper
• ER quality control: towards an understanding at the molecular level.

  2001cited by this paper
• Galactose-extended glycans of antibodies produced by transgenic plants

  2001influential reference
• Putting tumours in context

  2001cited by this paper
• Influence of growth conditions and developmental stage on N-glycan heterogeneity of transgenic immunoglobulin G and endogenous proteins in tobacco leaves.

  2001cited by this paper
• Influence of KDEL on the Fate of Trimeric or Assembly-Defective Phaseolin: Selective Use of an Alternative Route to Vacuoles

  2001cited by this paper
• Sorting of Glycoprotein B from Human Cytomegalovirus to Protein Storage Vesicles in Seeds of Transgenic Tobacco

  2001cited by this paper
• Native and artificial reticuloplasmins co-accumulate in distinct domains of the endoplasmic reticulum and in post-endoplasmic reticulum compartments.

  2001cited by this paper
• A murine monoclonal antibody produced in transgenic plants with plant-specific glycans is not immunogenic in mice

  2000cited by this paper
• Biogenesis of the Protein Storage Vacuole Crystalloid

  2000cited by this paper
• Mass Transport of Proform of a Kdel-Tailed Cysteine Proteinase (Sh-EP) to Protein Storage Vacuoles by Endoplasmic Reticulum–Derived Vesicle Is Involved in Protein Mobilization in Germinating Seeds

  2000cited by this paper
• Protein Recycling from the Golgi Apparatus to the Endoplasmic Reticulum in Plants and Its Minor Contribution to Calreticulin Retention

  2000cited by this paper
• β(1,2)-Xylose and α(1,3)-Fucose Residues Have a Strong Contribution in IgE Binding to Plant Glycoallergens*

  2000cited by this paper
• BiP and immunoglobulin light chain cooperate to control the folding of heavy chain and ensure the fidelity of immunoglobulin assembly.

  1999cited by this paper
• N-Glycosylation of a mouse IgG expressed in transgenic tobacco plants.

  1999cited by this paper
• Cis-elements of protein transport to the plant vacuoles

  1999cited by this paper
• Crystallographic structure of an intact IgG1 monoclonal antibody.

  1998cited by this paper
• Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity

  1998influential reference
• N-Glycoprotein biosynthesis in plants: recent developments and future trends

  1998cited by this paper
• Deposition of storage proteins

  1998influential reference
• Transport of Storage Proteins to Protein Storage Vacuoles Is Mediated by Large Precursor-Accumulating Vesicles

  1998cited by this paper
• N-glycans harboring the Lewis a epitope are expressed at the surface of plant cells.

  1997cited by this paper
• The C-terminal HDEL sequence is sufficient for retention of secretory proteins in the endoplasmic reticulum (ER) but promotes vacuolar targeting of proteins that escape the ER.

  1997cited by this paper
• Production of recombinant proteins in transgenic plants: Practical considerations

  1997cited by this paper
• Optimization of scFv antibody production in transgenic plants.

  1997influential reference
• Limited proteolysis of beta-conglycinin and glycinin, the 7S and 11S storage globulins from soybean [Glycine max (L.) Merr.]. Structural and evolutionary implications.

  1996cited by this paper
• The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco

  1996cited by this paper
• Limited Proteolysis of β‐Conglycinin and Glycinin, the 7S and 11S Storage Globulins from Soybean [Glycine Max (L.) Merr.]

  1996cited by this paper
• Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid.

  1995cited by this paper
• Seed-Specific Gene Activation Mediated by the Cre/lox Site-Specific Recombination System

  1994cited by this paper
• Antibody catalyzed hydrolysis of enol ethers

  1993cited by this paper
• Affinity purification of antibodies specific for Asn-linked glycans containing alpha 1-->3 fucose or beta 1-->2 xylose.

  1993cited by this paper
• Affinity Purification of Antibodies Specific for Asn-Linked Glycans Containing α1 → 3 Fucose or β1 → 2 Xylose

  1993cited by this paper
• Effects of sulfur nutrition on expression of the soybean seed storage protein genes in transgenic petunia.

  1992cited by this paper
• Vicilin with carboxy-terminal KDEL is retained in the endoplasmic reticulum and accumulates to high levels in the leaves of transgenic plants.

  1992cited by this paper
• Diversification of the IgG molecule by oligosaccharides.

  1991cited by this paper
• Retention of phytohemagglutinin with carboxyterminal tetrapeptide KDEL in the nuclear envelope and the endoplasmic reticulum

  1990cited by this paper
• Nuclear transport of plant potyviral proteins.

  1990cited by this paper
• Production of antibodies in transgenic plants

  1989cited by this paper
• Regulated expression of genes encoding soybean beta-conglycinins in transgenic plants.

  1989cited by this paper
• Leaf disc transformation

  1989cited by this paper
• Common antigenic determinants in the glycoproteins of plants, molluscs and insects

  1988cited by this paper
• Immunoglobulin G as a glycoprotein.

  1986cited by this paper
• Functional analysis of regulatory elements in a plant embryo-specific gene.

  1986cited by this paper
• Characterization of N-linked oligosaccharides by affinoblotting with concanavalin A-peroxidase and treatment of the blots with glycosidases.

  1985cited by this paper
• Immunoglobulin heavy chain binding protein

  1983cited by this paper

• Protein‐Derived Signal Peptides Induced by Agrobacterium Infection Promote the Secretion of Recombinant Proteins in Nicotiana benthamiana

  2025cites this paper
• A Kaleidoscope of Hosts: Expression Systems of Recombinant Antibody Reagents for Immunological Assays.

  2025cites this paper
• Optimized expression of human interleukin-15 in Nicotiana benthamiana and in vitro assessment of its activity on human keratinocytes

  2025cites this paper
• AtLEC2‐Mediated Enhancement of Endoplasmic Reticulum‐Targeted Foreign Protein Synthesis in Nicotiana benthamiana Leaves: Insights From Transcriptomic Analysis

  2024cites this paper
• Characterization of plant produced VHH antibodies against cobra venom toxins for antivenom therapy

  2024cites this paper
• Quality control in SARS-CoV-2 RBD-Fc vaccine production using LC–MS to confirm strain selection and detect contaminations from other strains

  2024cites this paper
• Hairy roots as a platform for recombinant protein expression and secondary metabolite production: current status and future prospects

  2024cites this paper
• Soybean seed protein storage vacuoles for expression of recombinant molecules.

  2023cites this paper
• In vitro and in vivo studies of plant-produced Atezolizumab as a potential immunotherapeutic antibody

  2023cites this paper
• Impact of mutations on the plant-based production of recombinant SARS-CoV-2 RBDs

  2023cites this paper
• Production of enzymes for the removal of odorous substances in plant biomass.

  2023cites this paper
• The tobacco GNTI stem region harbors a strong motif for homomeric protein complex formation

  2023cites this paper
• Feasibility of plant-expression system for production of recombinant anti-human IgE: An alternative production platform for therapeutic monoclonal antibodies

  2022influential citation
• Improving Protein Quantity and Quality—The Next Level of Plant Molecular Farming

  2022cites this paper
• Synthesis of single‐chain antibody fragment fused to the elastin‐like polypeptide in Nicotiana benthamiana and its application in affinity precipitation of difficult to produce proteins

  2022cites this paper
• Increasing the Efficiency of the Accumulation of Recombinant Proteins in Plant Cells: The Role of Transport Signal Peptides

  2022cites this paper
• Functional Characterization of Pembrolizumab Produced in Nicotiana benthamiana Using a Rapid Transient Expression System

  2021cites this paper
• N-Glycosylation of the SARS-CoV-2 Receptor Binding Domain Is Important for Functional Expression in Plants

  2021cites this paper
• Expression and Functional Evaluation of Recombinant Anti-receptor Activator of Nuclear Factor Kappa-B Ligand Monoclonal Antibody Produced in Nicotiana benthamiana

  2021cites this paper
• Impact of Specific N-Glycan Modifications on the Use of Plant-Produced SARS-CoV-2 Antigens in Serological Assays

  2021cites this paper
• Dually localized proteins found in both the apicoplast and mitochondrion utilize the Golgi-dependent pathway for apicoplast targeting in Toxoplasma gondii

  2020cites this paper
• Expression optimization, purification and in vitro characterization of human epidermal growth factor produced in Nicotiana benthamiana

  2020cites this paper
• Dually localised proteins found in both the apicoplast and mitochondrion utilize the Golgi‐dependent pathway for apicoplast targeting in Toxoplasma gondii

  2020cites this paper
• CRISPR/Cas9-based Antibody Production in Plants

  2020cites this paper
• Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update

  2020cites this paper
• Structural and In Vitro Functional Analyses of Novel Plant-Produced Anti-Human PD1 Antibody

  2019cites this paper
• Estudio del efecto de genes involucrados en el desarrollo de la vía secretoria y su aplicación a la producción de proteínas recombinantes en células vegetales

  2019influential citation
• Plant Platform for Therapeutic Monoclonal Antibody Production

  2019cites this paper
• Etude et ingénierie de la N-glycosylation des protéines chez la microalgue verte chlamydomanas reinhardtii.

  2019cites this paper
• Production of recombinant colicin M in Nicotiana tabacum plants and its antimicrobial activity

  2019cites this paper
• Posttranslational Modification of Heterologous Human Therapeutics in Plant Host Expression Systems

  2019cites this paper
• Cis/Transgene Optimization

  2018cites this paper
• Tanaman Sebagai Bioreaktor Protein Farmaseutik

  2018cites this paper
• Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana

  2018cites this paper
• The use of legume seed for expression and storage of high value proteins.

  2018cites this paper
• Hydroponic Treatment of Nicotiana benthamiana with Kifunensine Modifies the N-glycans of Recombinant Glycoprotein Antigens to Predominantly Man9 High-Mannose Type upon Transient Overexpression

  2018cites this paper
• Transgenesis and Plant Molecular Pharming

  2017cites this paper
• High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza

  2017cites this paper
• Immunogenicity of glycans on biotherapeutic drugs produced in plant expression systems—The taliglucerase alfa story

  2017cites this paper
• Sugarcane: An Efficient Platform for Molecular Farming

  2017cites this paper
• Recombinant therapeutic proteins produced in plants: towards engineering of human-type O-and N-glycosylation

  2016cites this paper
• The N-glycan on Asn54 affects the atypical N-glycan composition of plant-produced interleukin-22, but does not influence its activity.

  2016cites this paper
• Cis/transgene optimization: systematic discovery of some key gene expression elements integrating bioinformatics and computational biology

  2016cites this paper
• Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications

  2016cites this paper
• Vacuolar targeting of recombinant antibodies in Nicotiana benthamiana

  2016cites this paper
• Plant factories for the production of monoclonal antibodies

  2016cites this paper
• Plant Molecular Pharming

  2016cites this paper
• Plants as Factories for Human Pharmaceuticals: Applications and Challenges

  2015cites this paper
• Biochemical Characterization of Human Anti-Hepatitis B Monoclonal Antibody Produced in the Microalgae Phaeodactylum tricornutum

  2015cites this paper
• Subcellular Targeting of Proteins Involved in Modification of Plant N- and O-Glycosylation.

  2015cites this paper
• UDP-d-galactose synthesis by UDP-glucose 4-epimerase 4 is required for organization of the trans-Golgi network/early endosome in Arabidopsis thaliana root epidermal cells

  2015cites this paper
• HIGH-LEVEL EXPRESSION AND PURIFICATION OF A THERAPEUTIC RECOMBINANT SERINE PROTEASE INHIBITOR FROM TRANSGENIC TOMATO PLANTS

  2015cites this paper
• Tobacco seeds as efficient production platform for a biologically active anti-HBsAg monoclonal antibody

  2015cites this paper
• Expression of bioactive anti-CD20 antibody fragments and induction of ER stress response in Arabidopsis seeds

  2015cites this paper
• Production of the Main Celiac Disease Autoantigen by Transient Expression in Nicotiana benthamiana

  2015cites this paper
• Comparison of VHH-Fc antibody production in Arabidopsis thaliana, Nicotiana benthamiana and Pichia pastoris.

  2015cites this paper
• N-Glycosylation Modification of Plant-Derived Virus-Like Particles: An Application in Vaccines

  2014cites this paper
• Trafficking of endoplasmic reticulum-retained recombinant proteins is unpredictable in Arabidopsis thaliana

  2014influential citation
• Journal of Immunological Techniques in Infectious Diseases

  2014cites this paper
• State of the Art on Plant-Made Single-Domain Antibodies

  2014cites this paper
• Vaccine production and glycoengineering in Arabidopsis thaliana seeds

  2014cites this paper
• Glyco-engineering for biopharmaceutical production in moss bioreactors

  2014cites this paper
• Expression of Aβ-Fc fusion protein in transgenic potato.

  2014cites this paper
• The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2‐L bioreactor

  2014cites this paper
• On the way to commercializing plant cell culture platform for biopharmaceuticals: present status and prospect.

  2014cites this paper
• Mathematical Model for Production of Recombinant Antibody 14 D 9 By Nicotiana tabacum Cell Suspension Batch Culture

  2014cites this paper
• The dynamic behavior of storage organelles in developing cereal seeds and its impact on the production of recombinant proteins

  2014cites this paper
• Production of cecropin A antimicrobial peptide in rice seed endosperm

  2014cites this paper
• Tobacco shoot regeneration from calli in temporary immersion culture for biosynthesis of heterologous biopharmaceuticals

  2014cites this paper
• Mathematical Modelling in Recombinant Plant Systems: The Challenge to Produce Heterologous Proteins Under GLP/GMP

  2014cites this paper
• The Antibody 14D9 as an Experimental Model for Molecular Farming

  2014cites this paper
• 13_13-154_Kisung Ko.hwp

  2014cites this paper
• The human anti-HIV antibodies 2F5, 2G12, and PG9 differ in their susceptibility to proteolytic degradation: Down-regulation of endogenous serine and cysteine proteinase activities could improve antibody production in plant-based expression platforms

  2014cites this paper
• Induction of recombinant proteins in wheat seed and vegetative tissues

  2013cites this paper
• ER stress response induced by the production of human IL-7 in rice endosperm cells

  2013cites this paper
• Role of plant expression systems in antibody production for passive immunization.

  2013cites this paper
• Glycomodification and characterization of anti-colorectal cancer immunotherapeutic monoclonal antibodies in transgenic tobacco

  2013cites this paper
• Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants

  2013cites this paper
• N-glycosylation of plant-produced recombinant proteins.

  2013cites this paper
• Seeds as a production system for molecular pharming applications: status and prospects.

  2013cites this paper
• Green factories for biopharmaceuticals.

  2013cites this paper
• Molecular farming of pharmaceutical proteins using plant suspension cell and tissue cultures.

  2013cites this paper
• Intracellular Reprogramming of Expression, Glycosylation, and Function of a Plant-Derived Antiviral Therapeutic Monoclonal Antibody

  2013cites this paper
• Maize seeds as a production and delivery platform for HIV microbicides

  2013cites this paper
• Running title: Giant ER-derived PB contains modified Bet v 1

  2013cites this paper
• Transgenic rice seeds accumulating recombinant hypoallergenic birch pollen allergen Bet v 1 generate giant protein bodies.

  2013cites this paper
• Plant species and organ influence the structure and subcellular localization of recombinant glycoproteins

  2013cites this paper
• Recombinant Antibody Production in Arabidopsis Seeds Triggers an Unfolded Protein Response1[W][OA]

  2012cites this paper
• PLANT-DERIVED ANTIBODIES FOR ACADEMIC, INDUSTRIAL, AND THERAPEUTIC APPLICATIONS

  2012cites this paper
• Tackling Heterogeneity: A Leaf Disc-Based Assay for the High-Throughput Screening of Transient Gene Expression in Tobacco

  2012cites this paper
• Characterization and mapping of a putative laccase-like multicopper oxidase gene in the barley (Hordeum vulgare L.).

  2012cites this paper
• Production of different glycosylation variants of the tumour-targeting mAb H10 in Nicotiana benthamiana: influence on expression yield and antibody degradation

  2012cites this paper
• Moss-based production of asialo-erythropoietin devoid of Lewis A and other plant-typical carbohydrate determinants.

  2012cites this paper
• CLONING TRANSFORMATION AND EXPRESSION OF cDNA OF HUMAN scFv AGAINST HEPATOCELLULAR CARCINOMA IN INDICA RICE VARIETY IR 64

  2012cites this paper
• High-Level Transient Expression of ER-Targeted Human Interleukin 6 in Nicotiana benthamiana

  2012cites this paper
• Production of α-L-iduronidase in maize for the potential treatment of a human lysosomal storage disease

  2012cites this paper
• Expression of the VP1 antigen from foot-and-mouth disease virus in a bacterial and plant-based expression system

  2012cites this paper
• Influence of an ER-retention signal on the N-glycosylation of recombinant human α-l-iduronidase generated in seeds of Arabidopsis

  2012influential citation
• Microalgae, Functional Genomics and Biotechnology

  2012cites this paper
• Seed Expression Systems for Molecular Farming

  2012cites this paper