OptoDyCE: Automated System for High-Throughput All-Optical Dynamic Cardiac Electrophysiology

No abstract is available for this paper.

• Publication year

  2015

• Venue

  bioRxiv

• Publication date

  2015-07-31

• Fields of study

  Biology, Medicine, Computer Science, Engineering

• Identifiers
  DOI 10.1117/12.2212736
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• A New Perspective in the Field of Cardiac Safety Testing through the Comprehensive In Vitro Proarrhythmia Assay Paradigm

  2016cited by this paper
• Optogenetic versus Electrical Stimulation of Human Cardiomyocytes: Modeling Insights.

  2015cited by this paper
• Rapid Cellular Phenotyping of Human Pluripotent Stem Cell-Derived Cardiomyocytes using a Genetically Encoded Fluorescent Voltage Sensor

  2014cited by this paper
• Cardiac applications of optogenetics.

  2014cited by this paper
• Optogenetic control of cardiomyocytes via viral delivery.

  2014cited by this paper
• All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins

  2014cited by this paper
• Pluripotent Stem Cells as a Platform for Cardiac Arrhythmia Drug Screening

  2014cited by this paper
• Refining the human iPSC-cardiomyocyte arrhythmic risk assessment model.

  2013cited by this paper
• Tools, methods, and applications for optophysiology in neuroscience

  2013cited by this paper
• Computational Optogenetics: Empirically-Derived Voltage- and Light-Sensitive Channelrhodopsin-2 Model

  2013cited by this paper
• Targeting neurons and photons for optogenetics

  2013cited by this paper
• Drug-induced functional cardiotoxicity screening in stem cell-derived human and mouse cardiomyocytes: effects of reference compounds.

  2013cited by this paper
• Cardiac optogenetics.

  2013cited by this paper
• The case for induced pluripotent stem cell‐derived cardiomyocytes in pharmacological screening

  2013cited by this paper
• Variability in high-throughput ion-channel screening data and consequences for cardiac safety assessment

  2013cited by this paper
• Induced pluripotent stem cells: the new patient?

  2012cited by this paper
• Optical Imaging of Voltage and Calcium in Cardiac Cells & Tissues

  2012cited by this paper
• A comprehensive concept of optogenetics.

  2012cited by this paper
• Stimulating Cardiac Muscle by Light: Cardiac Optogenetics by Cell Delivery

  2011cited by this paper
• Simulation of multiple ion channel block provides improved early prediction of compounds’ clinical torsadogenic risk

  2011cited by this paper
• A Computational Model to Predict the Effects of Class I Anti-Arrhythmic Drugs on Ventricular Rhythms

  2011cited by this paper
• The Systems Biology Approach to Drug Development: Application to Toxicity Assessment of Cardiac Drugs

  2010cited by this paper
• Renaissance of ion channel research and drug discovery by patch clamp automation.

  2010cited by this paper
• Screening technologies for ion channel drug discovery.

  2010cited by this paper
• Effect of voltage sensitive fluorescent proteins on neuronal excitability.

  2009cited by this paper
• High-throughput electrophysiology: an emerging paradigm for ion-channel screening and physiology

  2008cited by this paper
• Spectral characteristics of the photocycle of channelrhodopsin-2 and its implication for channel function.

  2008cited by this paper
• hERG (KCNH2 or Kv11.1) K+ channels: screening for cardiac arrhythmia risk.

  2008cited by this paper
• Modeling for Insights

  2007cited by this paper
• Near-infrared voltage-sensitive fluorescent dyes optimized for optical mapping in blood-perfused myocardium.

  2007cited by this paper
• The Role of Cardiac Tissue Alignment in Modulating Electrical Function

  2007cited by this paper
• A mathematical model of electrotonic interactions between ventricular myocytes and fibroblasts.

  2007cited by this paper
• Alternans and spiral breakup in a human ventricular tissue model.

  2006cited by this paper
• Population Patch Clamp Improves Data Consistency and Success Rates in the Measurement of Ionic Currents

  2006cited by this paper
• How many drug targets are there?

  2006cited by this paper
• Macroscopic optical mapping of excitation in cardiac cell networks with ultra-high spatiotemporal resolution.

  2006cited by this paper
• Channelrhodopsins: directly light-gated cation channels.

  2005cited by this paper
• Science, pharmacoeconomics and ethics in drug R&D: a sustainable future scenario?

  2004cited by this paper
• Determination of electrical properties of ES cell-derived cardiomyocytes using MEAs.

  2004cited by this paper
• Channelrhodopsin-2, a directly light-gated cation-selective membrane channel

  2003cited by this paper
• Relationships between preclinical cardiac electrophysiology, clinical QT interval prolongation and torsade de pointes for a broad range of drugs: evidence for a provisional safety margin in drug development.

  2003cited by this paper
• Highly sensitive fluorescence detection technology currently available for HTS.

  2003cited by this paper
• A guide to drug discovery: Trends in development and approval times for new therapeutics in the United States

  2003cited by this paper
• IonWorks™ HT: A New High-Throughput Electrophysiology Measurement Platform

  2003cited by this paper
• Adaptation of Aequorin Functional Assay to High Throughput Screening

  2002cited by this paper
• FLIPR: A New Instrument for Accurate, High Throughput Optical Screening

  1996cited by this paper
• Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Estimation of Action Potential Changes from Field Potential Recordings in Multicellular Mouse Cardiac Myocyte Cultures Key Words Microelectrode Array @bullet Action Potential Upstroke @bullet Action P

  year unknowncited by this paper

• Unpinning and elimination of spiral waves: A machine learning-based method

  2026cites this paper
• Voltage sensitive probes for membrane potential determination in life science

  2026cites this paper
• Paired single-cell imaging of calcium and expression to map niches of identity and function

  2026cites this paper
• Low-dimensional materials for intracellular electrophysiology: advances from synthesis to applications

  2026cites this paper
• Three-Dimensional-Printed Flexible Nanosilver Electrode Array for Parallel and Robust Intracellular Electrophysiological Recording.

  2025cites this paper
• Rigid and soft microelectrodes for electrophysiology measurement

  2025cites this paper
• High-Throughput Cardiomyocytes Biosensing: From Single-Parameter Detection to Integrated Mechano-Electrophysiological Platforms

  2025cites this paper
• A universal, accurate natural compounds evaluation platform via cardiomyocyte based biosensing

  2025cites this paper
• Probing relaxed myosin states in hypertrophic cardiomyopathy by second harmonic-generation microscopy

  2025cites this paper
• Robust Thermally Oxidized ZnO Nanowire Electrodes for High-Fidelity Intracellular Recording and Pharmacological Assessment.

  2025cites this paper
• Response of human iPSC-cardiomyocytes to adrenergic drugs assessed by high-throughput pericellular oxygen measurements

  2025cites this paper
• Two Decades of Optogenetic Tools: A Retrospective and a Look Ahead

  2025cites this paper
• Engineering brain-on-a-chip platforms

  2024cites this paper
• Optogenetic stimulation and simultaneous optical mapping of membrane potential and calcium transients in human engineered cardiac spheroids.

  2024cites this paper
• Native mechano-regulative matrix properties stabilize alternans dynamics and reduce spiral wave stabilization in cardiac tissue

  2024cites this paper
• Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) for modeling cardiac arrhythmias: strengths, challenges and potential solutions

  2024cites this paper
• Integrated nanoporous electroporation and sensing electrode array for total dynamic time-domain cardiomyocyte membrane resealing assessment

  2024cites this paper
• Control of spiral waves in myocardial tissue by optogenetics and temperature

  2024cites this paper
• Micronano Synergetic Three-Dimensional Bioelectronics: A Revolutionary Breakthrough Platform for Cardiac Electrophysiology.

  2024cites this paper
• A fully-automated low-cost cardiac monolayer optical mapping robot

  2023cites this paper
• Simultaneous Widefield Voltage and Dye-Free Optical Mapping Quantifies Electromechanical Waves in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

  2023cites this paper
• CRISPRi Gene Modulation and All-Optical Electrophysiology in Post-Differentiated Human iPSC-Cardiomyocytes

  2023cites this paper
• Cardiac optogenetics: shining light on signaling pathways

  2023cites this paper
• Elevating intracellular action potential recording in cardiomyocytes: A precision-enhanced and biosafe single-pulse electroporation system.

  2023cites this paper
• A micro-LED array based platform for spatio-temporal optogenetic control of various cardiac models

  2023cites this paper
• OptoDyCE-plate as an affordable high throughput imager for all optical cardiac electrophysiology

  2023influential citation
• Three-Dimensional Cardiomyocyte-Nanobiosensing System for Specific Recognition of Drug Subgroups.

  2023cites this paper
• CRISPRi gene modulation and all-optical electrophysiology in post-differentiated human iPSC-cardiomyocytes

  2023influential citation
• Human iPSC-Cardiomyocytes as an Experimental Model to Study Epigenetic Modifiers of Electrophysiology

  2022influential citation
• Animal models and animal-free innovations for cardiovascular research: current status and routes to be explored. Consensus document of the ESC working group on myocardial function and the ESC Working Group on Cellular Biology of the Heart.

  2022cites this paper
• Photoelectrochemical imaging of single cardiomyocytes and monitoring of their action potentials through contact force manipulation of organoids.

  2022cites this paper
• Scalable and Robust Hollow Nanopillar Electrode for Enhanced Intracellular Action Potential Recording.

  2022cites this paper
• Heart-on-a-chip platforms and biosensor integration for disease modeling and phenotypic drug screening.

  2022cites this paper
• Simultaneous widefield voltage and interferometric dye-free optical mapping quantifies electromechanical waves in human iPSC-cardiomyocytes

  2022cites this paper
• Editorial: Cardiac optogenetics: Using light to observe and excite the heart

  2022cites this paper
• Flexible Electro‐Optical Arrays for Simultaneous Multi‐Site Colocalized Spatiotemporal Cardiac Mapping and Modulation

  2022cites this paper
• Photoelectrochemical imaging of action potentials for single cardiomyocytes through contact force manipulation of organoids

  2022cites this paper
• Scalable Nanotrap Matrix Enhanced Electroporation for Intracellular Recording of Action Potential.

  2022cites this paper
• Spiral wave drift under optical feedback in cardiac tissue.

  2022cites this paper
• Optogenetics for light control of biological systems

  2022cites this paper
• Opportunities and challenges in cardiac tissue engineering from an analysis of two decades of advances

  2022cites this paper
• Portable low-cost macroscopic mapping system for all-optical cardiac electrophysiology

  2022cites this paper
• Optogenetics in cardiology: methodology and future applications

  2022cites this paper
• Porous Polyethylene Terephthalate Nanotemplate Electrodes for Sensitive Intracellular Recording of Action Potentials.

  2022cites this paper
• Optogenetic Control of Human Induced Pluripotent Stem Cell‐Derived Cardiac Tissue Models

  2022cites this paper
• Optophysiology: Illuminating cell physiology with optogenetics

  2022cites this paper
• Random access parallel microscopy

  2021cites this paper
• Novel Optics-Based Approaches for Cardiac Electrophysiology: A Review

  2021cites this paper
• Optogenetic Stimulation of Primary Cardiomyocytes Expressing ChR2.

  2021cites this paper
• Drug Discovery in Induced Pluripotent Stem Cell Models

  2021cites this paper
• A Human 3D Cardiomyocyte Risk Model to Study the Cardiotoxic Influence of X-rays and Other Noxae in Adults

  2021cites this paper
• OptoGap is an optogenetics-enabled assay for quantification of cell–cell coupling in multicellular cardiac tissue

  2021cites this paper
• Integration of Engineered “Spark-Cell” Spheroids for Optical Pacing of Cardiac Tissue

  2021cites this paper
• A model-guided pipeline for drug cardiotoxicity screening with human stem-cell derived cardiomyocytes

  2021cites this paper
• Fast Optical Investigation of Cardiac Electrophysiology by Parallel Detection in Multiwell Plates

  2021cites this paper
• Optogenetic Stimulation Using Anion Channelrhodopsin (GtACR1) Facilitates Termination of Reentrant Arrhythmias With Low Light Energy Requirements: A Computational Study

  2021cites this paper
• Advanced Optogenetic-Based Biosensing and Related Biomaterials

  2021cites this paper
• CRISPR/Cas9 technologies to manipulate human induced pluripotent stem cells

  2021cites this paper
• Linearly polarized excitation enhances signals from fluorescent voltage indicators

  2021cites this paper
• COSMAS: a lightweight toolbox for cardiac optical mapping analysis

  2021cites this paper
• Optogenetic Cardiac Pacing

  2021cites this paper
• Intracellular recording of cardiomyocyte action potentials by nanobranched microelectrode array.

  2020cites this paper
• Recent progress in optical voltage-sensor technology and applications to cardiac research: from single cells to whole hearts.

  2020cites this paper
• No light without the dark: Perspectives and hindrances for translation of cardiac optogenetics.

  2020cites this paper
• Optogenetics: Background, Methodological Advances and Potential Applications for Cardiovascular Research and Medicine

  2020cites this paper
• Leave the Light On: Chronic Optogenetic Tachypacing of Human Engineered Cardiac Tissue Constructs.

  2020cites this paper
• All-Optical Electrophysiology Refines Populations of In Silico Human iPSC-CMs for Drug Evaluation

  2020cites this paper
• Advances in Stem Cell Modeling of Dystrophin-Associated Disease: Implications for the Wider World of Dilated Cardiomyopathy

  2020cites this paper
• CARDIAC SAFETY SCREENING WHITE PAPER

  2020cites this paper
• Microfluidics-enabled 96-well perfusion system for high-throughput tissue engineering and long-term all-optical electrophysiology

  2020cites this paper
• Combining tissue engineering and optical imaging approaches to explore interactions along the neuro-cardiac axis

  2020cites this paper
• Calcium imaging and optical electrophysiology

  2020cites this paper
• All-Optical Miniaturized Co-culture Assay of Voltage-Gated Ca2+ Channels.

  2020cites this paper
• Monitoring contractility in cardiac tissue with cellular resolution using biointegrated microlasers

  2020cites this paper
• Optical Interrogation of Sympathetic Neuronal Effects on Macroscopic Cardiomyocyte Network Dynamics

  2020cites this paper
• Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology

  2020cites this paper
• Assessment of Cardiotoxicity With Stem Cell-Based Strategies.

  2020cites this paper
• Syncytium cell growth increases Kir2.1 contribution in human iPSC-cardiomyocytes

  2020cites this paper
• Lab on a Chip Microfluidics-enabled 96-well perfusion system for high-throughput tissue engineering and long-term all-optical electrophysiology †

  2020cites this paper
• Optogenetic Control of the BMP Signaling Pathway

  2020cites this paper
• Optogenetics for cardiac pacing, resynchronization, and arrhythmia termination

  2020cites this paper
• A Scalable Approach Reveals Functional Responses of iPSC Cardiomyocyte 3D Spheroids

  2020cites this paper
• Optogenetic Pacing: Current Insights and Future Potential

  2020cites this paper
• Arrhythmia mechanisms in human induced pluripotent stem cell-derived cardiomyocytes.

  2020cites this paper
• Parallel All-Optical Assay to Study Use-Dependent Functioning of Voltage-Gated Ion Channels in a Miniaturized Format

  2020cites this paper
• Cardiac optogenetics: a decade of enlightenment

  2020cites this paper
• University of Dundee Monitoring contractility in cardiac tissue with cellular resolution using biointegrated

  2020cites this paper
• Feasibility of Using Adjunctive Optogenetic Technologies in Cardiomyocyte Phenotyping – from the Single Cell to the Whole Heart

  2020influential citation
• A ‘comb’ algorithm for accurate detection of calcium transients and action potentials in regularly activating cardiac preparations

  2019cites this paper
• Optical interrogation of sympathetic neuronal effects on macroscopic cardiac monolayer dynamics

  2019influential citation
• Novel method for action potential measurements from intact cardiac monolayers with multiwell microelectrode array technology

  2019cites this paper
• Electrophysiological characterization of drug response in hSC-derived cardiomyocytes using voltage-sensitive optical platforms.

  2019cites this paper
• Stem Cells in Cardiovascular Medicine: Historical Overview and Future Prospects

  2019cites this paper
• Optogenetic Termination of Cardiac Arrhythmia: Mechanistic Enlightenment and Therapeutic Application?

  2019cites this paper
• A Light Wand to Untangle the Myocardial Cell Network

  2019cites this paper
• Automatic assessment of the cardiomyocyte development stages from confocal microscopy images using deep convolutional networks

  2019cites this paper
• Monitoring contractility in single cardiomyocytes and whole hearts with bio-integrated microlasers

  2019cites this paper
• University of Birmingham Cardiac optogenetics and optical mapping – overcoming spectral congestion in all-optical cardiac electrophysiology

  2019influential citation
• Light and the Heart

  2019cites this paper
• Solid State High Throughput Screening Microscopy

  2019cites this paper