Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes

No abstract is available for this paper.

• Publication year

  2014

• Venue

  Nature Methods

• Publication date

  2014-01-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/nmeth.2773PMID 24390440
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Ion channels

  2013cited by this paper
• The Revolution Continues

  2013cited by this paper
• Real-time in vivo analysis of T cell activation in the central nervous system using a genetically encoded calcium indicator

  2013cited by this paper
• Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics

  2013cited by this paper
• In vivo functional properties of juxtaglomerular neurons in the mouse olfactory bulb

  2013cited by this paper
• A Neuron-Based Screening Platform for Optimizing Genetically-Encoded Calcium Indicators

  2013cited by this paper
• Genetically encoded neural activity indicators.

  2012cited by this paper
• Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model

  2012cited by this paper
• New developments in the ATSAS program package for small-angle scattering data analysis

  2012cited by this paper
• Correlating calcium binding, Förster resonance energy transfer, and conformational change in the biosensor TN-XXL.

  2012cited by this paper
• Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging

  2012cited by this paper
• A Cre-Dependent GCaMP3 Reporter Mouse for Neuronal Imaging In Vivo

  2012cited by this paper
• Genetically Encoded Green Fluorescent Ca2+ Indicators with Improved Detectability for Neuronal Ca2+ Signals

  2012cited by this paper
• Functional immunoimaging: the revolution continues

  2012cited by this paper
• Genetically encoded optical indicators for the analysis of neuronal circuits

  2012cited by this paper
• Ion channels and transporters in lymphocyte function and immunity

  2012cited by this paper
• Nuclear pore complex composition: a new regulator of tissue-specific and developmental functions

  2012cited by this paper
• Design and application of genetically encoded biosensors.

  2011cited by this paper
• An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching

  2011cited by this paper
• An Expanded Palette of Genetically Encoded Ca2+ Indicators

  2011cited by this paper
• X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.

  2011cited by this paper
• Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano

  2010cited by this paper
• NMR Structure Determination for Larger Proteins Using Backbone-Only Data

  2010cited by this paper
• Bright cyan fluorescent protein variants identified by fluorescence lifetime screening

  2010cited by this paper
• Broadly tuned response properties of diverse inhibitory neuron subtypes in mouse visual cortex.

  2010cited by this paper
• Ensemble calculations of unstructured proteins constrained by RDC and PRE data: a case study of urea-denatured ubiquitin.

  2010cited by this paper
• In vivo imaging of partially reversible th17 cell-induced neuronal dysfunction in the course of encephalomyelitis.

  2010cited by this paper
• Structural Basis for Calcium Sensing by GCaMP2

  2009cited by this paper
• Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design

  2009cited by this paper
• Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators

  2009cited by this paper
• A genetically encoded calcium indicator for chronic in vivo two-photon imaging

  2008cited by this paper
• Consistent blind protein structure generation from NMR chemical shift data

  2008cited by this paper
• Abeta plaques lead to aberrant regulation of calcium homeostasis in vivo resulting in structural and functional disruption of neuronal networks.

  2008cited by this paper
• Highly Selective Receptive Fields in Mouse Visual Cortex

  2008cited by this paper
• Genetically encoded calcium indicators.

  2008cited by this paper
• Structural basis for calcium sensing by GCaMP2.

  2008cited by this paper
• MolProbity: all-atom contacts and structure validation for proteins and nucleic acids

  2007cited by this paper
• ATSAS 2.1, a program package for small‐angle scattering data analysis

  2006cited by this paper
• Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs.

  2006cited by this paper
• A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change.

  2006cited by this paper
• Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex

  2005cited by this paper
• Monitoring Neural Activity and [Ca2+] with Genetically Encoded Ca2+ Indicators

  2004cited by this paper
• Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins.

  2004cited by this paper
• The Computer Aided Resonance Assignment Tutorial

  2004cited by this paper
• Genetically Encoded Indicators of Cellular Calcium Dynamics Based on Troponin C and Green Fluorescent Protein*

  2004cited by this paper
• UCSF Chimera—A visualization system for exploratory research and analysis

  2004cited by this paper
• In vivo two-photon calcium imaging of neuronal networks

  2003cited by this paper
• Uniqueness of ab initio shape determination in small-angle scattering

  2003cited by this paper
• A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications

  2002cited by this paper
• Use of chemical shifts in macromolecular structure determination.

  2002cited by this paper
• A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein

  2001cited by this paper
• Calmodulin bifurcates the local Ca2+ signal that modulates P/Q-type Ca2+ channels

  2001cited by this paper
• Using Situs for the registration of protein structures with low-resolution bead models from X-ray solution scattering

  2001cited by this paper
• Determination of domain structure of proteins from X-ray solution scattering.

  2001cited by this paper
• Regulation of contraction in striated muscle.

  2000cited by this paper
• Developmental profile and synaptic origin of early network oscillations in the CA1 region of rat neonatal hippocampus

  1998cited by this paper
• Defective TCR expression in transgenic mice constructed using cDNA‐based α‐ and β‐chain genes under the control of heterologous regulatory elements

  1998cited by this paper
• Tunable alignment of macromolecules by filamentous phage yields dipolar coupling interactions

  1998cited by this paper
• Crystal structure of troponin C in complex with troponin I fragment at 2.3-A resolution.

  1998cited by this paper
• Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra.

  1998cited by this paper
• Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin

  1997cited by this paper
• The Psychophysics Toolbox.

  1997cited by this paper
• The VideoToolbox software for visual psychophysics: transforming numbers into movies.

  1997cited by this paper
• AQUA and PROCHECK-NMR: Programs for checking the quality of protein structures solved by NMR

  1996cited by this paper
• MOLMOL: a program for display and analysis of macromolecular structures.

  1996cited by this paper
• NMR solution structure of calcium-saturated skeletal muscle troponin C.

  1995cited by this paper
• The revolution continues

  1995cited by this paper
• NMRPipe: A multidimensional spectral processing system based on UNIX pipes

  1995cited by this paper
• Quantitative J correlation: a new approach for measuring homonuclear three-bond J(HNH.alpha.) coupling constants in 15N-enriched proteins

  1993cited by this paper
• An efficient experiment for sequential backbone assignment of medium-sized isotopically enriched proteins

  1992cited by this paper
• Correlating Backbone Amide and Side-Chain Resonances in Larger Proteins By Multiple Relayed Triple Resonance NMR

  1992cited by this paper
• A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.

  1990cited by this paper
• A novel approach for sequential assignment of proton, carbon-13, and nitrogen-15 spectra of larger proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin

  1990cited by this paper
• Measurement of cytosolic free Ca2+ with quin2.

  1989cited by this paper
• Frontiers in Neural Circuits Neural Circuits Methods Article

  year unknowncited by this paper

• Unveiling FLNC variants: iPSC-derived myogenic cells as a model to study disease mechanisms.

  2026cites this paper
• The Multifaceted Role of Calcium Signaling in Regulated Necrosis

  2025cites this paper
• Multicolor Biosensors for Early Diagnosis of Diseases.

  2025cites this paper
• Directed Evolution of Fluorescent Genetically Encoded Biosensors: Innovative Approaches for Development and Optimization of Biosensors

  2025cites this paper
• From resting potential to dynamics: advances in membrane voltage indicators and imaging techniques

  2025influential citation
• Energetic diversity in retinal ganglion cells is modulated by neuronal activity and correlates with resilience to degeneration

  2025cites this paper
• T-B cell cooperation in ectopic lymphoid follicles propagates CNS autoimmunity.

  2025cites this paper
• Structure Prediction of Alternate Frame Folding Systems with AlphaFold3

  2025cites this paper
• Neural stem cell quiescence and activation dynamics are regulated by feedback input from their progeny under homeostatic and regenerative conditions.

  2025cites this paper
• Intracellular Ca2+ waves in mammalian cells

  2025cites this paper
• Abundance-biased codon diversification prevents recombination in AAV production and ensures robust in vivo expression of functional FRET sensors

  2025cites this paper
• “Enkephalinergic Neurons Gate Sex-Specific Control of Voluntary Micturition”

  2025cites this paper
• Enkephalinergic Neurons in Barrington’s Nucleus Gate Sex-Specific Control of Micturition

  2025cites this paper
• Regulatory Action of Calcium and Calcium Channels in Pain Pathways

  2025cites this paper
• R103 and R115 Affinity Mutants of ATeam ATP Biosensors

  2025cites this paper
• Anomalous photophysical behaviors attributed to excitonic coupling in fluorescent proteins.

  2025cites this paper
• Live-cell quantitative FRET imaging made simple by autocalibration in QuanTI-FRET

  2025cites this paper
• Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts

  2024cites this paper
• Tongue-on-a-Chip: Parallel Recording of Sweet and Bitter Receptor Responses to Sequential Injections of Pure and Mixed Sweeteners

  2024cites this paper
• Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals

  2024cites this paper
• Development of novel indicators and molecular systems for calcium sensing through protein engineering.

  2024cites this paper
• Calcium Indicators with Fluorescence Lifetime-Based Signal Readout: A Structure–Function Study

  2024cites this paper
• Multimodal segmentation of dynamic subcellular features using quantitative phase imaging and FRET-based sensors [Invited].

  2024cites this paper
• Ratiometric Imaging of Catecholamine Neurotransmitters with Nanosensors.

  2024cites this paper
• Suppression of Contraction Raises Calcium Ion Levels in the Heart of Zebrafish Larvae

  2024influential citation
• A-Syn(ful) MAM: A Fresh Perspective on a Converging Domain in Parkinson’s Disease

  2024cites this paper
• Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism.

  2024cites this paper
• Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight

  2024cites this paper
• Advances in optical recording techniques for non-invasive monitoring of electrophysiological signals

  2024cites this paper
• A Modular Biosensor Design for Quantitative Measurement of Free Nedd8

  2024cites this paper
• Probing neuronal activity with genetically encoded calcium and voltage fluorescent indicators.

  2024cites this paper
• Life history of a brain autoreactive T cell: From thymus through intestine to blood-brain barrier and brain lesion

  2024cites this paper
• Improvement of the Green–Red Förster Resonance Energy Transfer-Based Ca2+ Indicator by Using the Green Fluorescent Protein, Gamillus, with a Trans Chromophore as the Donor

  2024cites this paper
• Genetically Encoded Sensors for the In Vivo Detection of Neurochemical Dynamics.

  2024cites this paper
• Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.

  2024cites this paper
• Development of mKate3/HaloTag7 (JFX650) and CFP/YFP Dual-Fluorescence (or Förster) Resonance Energy Transfer Pairs for Visualizing Dual-Molecular Activity.

  2024cites this paper
• Electromechanical deformation of biological neurons: an intrinsic marker for label-free functional neuroimaging

  2024cites this paper
• Protocol to quantify the activation dynamics of tumor-associated T cells in mice by functional intravital microscopy

  2024cites this paper
• Angiotensin receptors and α1B-adrenergic receptors regulate native IK(ACh) and phosphorylation-deficient GIRK4 (S418A) channels through different PKC isoforms

  2024influential citation
• Horizontal-cell like Dm9 neurons in Drosophila modulate photoreceptor output to supply multiple functions in early visual processing

  2024cites this paper
• Enhanced detection sensitivity of neuronal activity patterns using CaMPARI1 vs. CaMPARI2

  2023cites this paper
• Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics

  2023cites this paper
• Immunomodulation under the lens of real‐time in vivo imaging

  2023cites this paper
• Subcellular second messenger networks drive distinct repellent-induced axon behaviors

  2023cites this paper
• Calcium signaling in plant immunity: a spatiotemporally controlled symphony.

  2023cites this paper
• CRISPR/Cas9 Genome Editing in LGMD2A/R1 Patient-Derived Induced Pluripotent Stem and Skeletal Muscle Progenitor Cells

  2023cites this paper
• ERG potassium channels and T‐type calcium channels contribute to the pacemaker and atrioventricular conduction in zebrafish larvae

  2023influential citation
• IN SITU IMAGING OF RETINAL CALCIUM DYNAMICS IN AWAKE ANIMALS

  2023cites this paper
• Ultrasonic reporters of calcium for deep tissue imaging of cellular signals

  2023cites this paper
• Calcium imaging: a technique to monitor calcium dynamics in biological systems

  2023cites this paper
• Ratiometric measurement of MAM Ca2+ dynamics using a modified CalfluxVTN

  2023influential citation
• Functional Imaging of Learning-Induced Plasticity in the Central Nervous System with Genetically Encoded Reporters in Drosophila.

  2023cites this paper
• Probing organoid metabolism using Fluorescence Lifetime Imaging Microscopy (FLIM): the next frontier of drug discovery and disease understanding.

  2023cites this paper
• Engineering NIR-II luminescent lanthanide nanoprobes for imaging brain diseases in vivo

  2023cites this paper
• Cross-pathway integration of cAMP signals through cGMP and calcium-regulated phosphodiesterases in mouse striatal cholinergic interneurons

  2023influential citation
• Early molecular layer interneuron hyperactivity triggers Purkinje neuron degeneration in SCA1

  2023cites this paper
• T:B cell cooperation in ectopic lymphoid follicles propagates CNS autoimmunity

  2023cites this paper
• Monitoring nutrients in plants with genetically encoded sensors: achievements and perspectives

  2023cites this paper
• Analysis of intracellular transport dynamics using quantitative phase imaging and FRET-based calcium sensors

  2023cites this paper
• Neurons on tape: Automated Tape Collecting Ultramicrotomy-mediated volume EM for targeting neuropathology.

  2023cites this paper
• Cellular and molecular imaging of CAR-T cell-based immunotherapy.

  2023cites this paper
• Biosensors integrated 3D organoid/organ-on-a-chip system: A real-time biomechanical, biophysical, and biochemical monitoring and characterization.

  2023cites this paper
• Diversity in homeostatic calcium set points predicts retinal ganglion cell survival following optic nerve injury in vivo

  2023cites this paper
• GCaMP, a Family of Single-Fluorophore Genetically Encoded Calcium Indicators

  2023cites this paper
• A Building Block Approach for the Total Synthesis of YM-385781.

  2023cites this paper
• Fluorescent sensors for imaging of interstitial calcium

  2023cites this paper
• Endogenous but not sensory-driven activity controls migration, morphogenesis and survival of adult-born juxtaglomerular neurons in the mouse olfactory bulb

  2023cites this paper
• Fluorescent Sensors for Imaging Interstitial Calcium

  2023cites this paper
• Neuronal silence as a prosurvival factor for adult-born olfactory bulb interneurons

  2023cites this paper
• Advances in NK cell therapy for brain tumors

  2023cites this paper
• Avoiding bias in fluorescence sensor readout

  2023cites this paper
• Targeting the TCA cycle can ameliorate widespread axonal energy deficiency in neuroinflammatory lesions

  2023cites this paper
• Neuroimaging with light field microscopy: a mini review of imaging systems

  2022cites this paper
• Smart genetically-encoded biosensors for the chemical monitoring of living systems.

  2022cites this paper
• A genetically encoded fluorescent sensor for manganese(II), engineered from lanmodulin

  2022cites this paper
• The Mycobacterium tuberculosis PE15/PPE20 complex transports calcium across the outer membrane

  2022influential citation
• cNTnC and fYTnC2, Genetically Encoded Green Calcium Indicators Based on Troponin C from Fast Animals

  2022cites this paper
• A general method for the development of multicolor biosensors with large dynamic ranges

  2022cites this paper
• Probing Neuropeptide Volume Transmission In Vivo by Simultaneous Near-Infrared Light Triggered Release and Optical Sensing.

  2022cites this paper
• Probing the interstitial calcium compartment

  2022cites this paper
• Spatiotemporal dynamics of calcium signals during neutrophil cluster formation

  2022cites this paper
• Cold-induced suspension and resetting of Ca2+ and transcriptional rhythms in the suprachiasmatic nucleus neurons

  2022cites this paper
• Glioblastoma hijacks neuronal mechanisms for brain invasion.

  2022cites this paper
• A BRET Ca2+ sensor enables high-throughput screening in the presence of background fluorescence

  2022cites this paper
• Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator

  2022influential citation
• GPCRs in Intracellular Compartments: New Targets for Drug Discovery

  2022cites this paper
• Calcium Imaging of Neural Activity in Fly Photoreceptors.

  2022cites this paper
• Calcium transport by the Mycobacterium tuberculosis PE15/PPE20 proteins

  2022influential citation
• GRID1/GluD1 homozygous variants linked to intellectual disability and spastic paraplegia impair mGlu1/5 receptor signaling and excitatory synapses

  2022cites this paper
• Hydrogel cultures reveal Transient Receptor Potential Vanilloid 4 regulation of myofibroblast activation and proliferation in valvular interstitial cells

  2022cites this paper
• Engineering rhodopsins' activation spectra using a FRET-based approach.

  2022cites this paper
• Fluorescent Indicators For Biological Imaging of Monatomic Ions

  2022cites this paper
• Fiber-based lactate recordings with fluorescence resonance energy transfer sensors by applying an magnetic resonance-informed correction of hemodynamic artifacts

  2022influential citation
• Chemical and molecular tools to probe biological sex differences at multiple length scales.

  2022cites this paper
• Genetically encoded fluorescent sensors for imaging neuronal dynamics in vivo

  2022cites this paper
• Resource for FRET-Based Biosensor Optimization

  2022cites this paper
• Neuroimaging with light field microscopy: a mini review of imaging systems

  2022cites this paper
• Simultaneous imaging of calcium and contraction in the beating heart of zebrafish larvae

  2022influential citation
• A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping

  2022influential citation
• Transcriptional Genetically Encoded Calcium Indicators in Drosophila.

  2022cites this paper