A simple, rapid and efficient method for the extraction of genomic DNA from lychee (Litchi chinensis Sonn.)

A simple, rapid and efficient method for isolating genomic DNA from lychee (Litchi chinensis Sonn.) was developed. This modified CTAB protocol include the use of 2 M NaCl, PVP, 5% mercaptoethanol and 80% ethanol in the extraction as well as reducing the centrifugation times during the separation and precipitation of the DNA. Isolated genomic DNA showed high purity and high quantity following restriction digestion analysis.

• Publication year

  2004

• Venue

  African Journal of Biotechnology

• Publication date

  2004-04-30

• Fields of study

  Biology

• Identifiers
  DOI 10.5897/AJB2004.000-2046
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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