Purification to homogeneity and amino acid sequence analysis of two anionic species of human interleukin 1

Two anionic species of human IL-1 have been purified to homogeneity. These molecules were characterized as having pI of 5.4 and 5.2 and molecular weights identical to IL-1/6.8 (17,500). The specific activities of IL-1/5.4 and IL-1/5.2, as measured in the mouse thymocyte co-mitogenic assay, were identical to that of IL-1/6.8, namely 1.2 X 10(7) U/mg, with half-maximal stimulation observed at 2 X 10(-11) M. IL- 1/5.4 and IL-1/5.2 were found to be antigenically distinct from IL- 1/6.8 in an ELISA. IL-1/5.4 was structurally distinct from IL-1/6.8 based on reverse-phase HPLC or CNBr peptides. Intact IL-1/5.2 and three intact CNBr peptides of IL-1/5.4 were sequenced, with the identification of 74 amino acid residues. These sequences were found to correspond exactly with the amino acid sequence deduced from the IL-1- alpha cDNA reported by March et al.

• Publication year

  1986

• Venue

  Journal of Experimental Medicine

• Publication date

  1986-07-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/JEM.164.1.237PMID 3487613PMCID 2188208
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Antibodies of predetermined specificity to the major charged species of human interleukin 1.

  1986cited by this paper
• Purification of human interleukin 1 from human monocyte culture supernatants and identity of thymocyte comitogenic factor, fibroblast-proliferation factor, acute-phase protein-inducing factor, and endogenous pyrogen.

  1985cited by this paper
• Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs

  1985influential reference
• Amino acid sequence analysis of human interleukin 1 (IL-1). Evidence for biochemically distinct forms of IL-1

  1985cited by this paper
• Biochemical heterogeneity of human interleukin-1.

  1985cited by this paper
• Pig interleukin 1. Purification of two immunologically different leukocyte proteins that cause cartilage resorption, lymphocyte activation, and fever

  1985influential reference
• Human interleukin 1. Purification to homogeneity

  1985cited by this paper
• The four biochemically distinct species of human interleukin 1 all exhibit similar biologic activities.

  1985cited by this paper
• Interleukin 1: an immunological perspective.

  1985cited by this paper
• Purification and partial sequence of human osteoclast-activating factor: identity with interleukin 1 beta.

  1985cited by this paper
• Biochemical heterogeneity of human interleukin-1.

  1985cited by this paper
• Purification to homogeneity of biologically active human interleukin 1.

  1984cited by this paper
• Purification of human interleukin 1 by high-performance liquid chromatography.

  1984cited by this paper
• Structural analysis of the charge heterogeneity of human interleukin 1.

  1984cited by this paper
• Cloning and expression of murine interleukin-1 cDNA in Escherichia coli

  1984influential reference
• Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.

  1984influential reference
• Purification and partial biochemical characterization of normal human interleukin 1

  1984influential reference
• Protein contaminants of sodium dodecyl sulfate-polyacrylamide gels.

  1983cited by this paper
• Purification to homogeneity of pig leucocyte catabolin, a protein that causes cartilage resorption in vitro.

  1983cited by this paper
• Interleukin 1, a potential regulator of fibroblast proliferation.

  1982cited by this paper
• Purification to apparent homogeneity of murine interleukin 1.

  1981cited by this paper
• Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.

  1981cited by this paper
• Physicochemical characterization of lymphocyte-activating factor (LAF).

  1979cited by this paper
• Partial purification of human lymphocyte-activating factor (LAF) by ultrafiltration and electrophoretic techniques.

  1977cited by this paper
• Deamidation of glutaminyl and asparaginyl residues in peptides and proteins.

  1974cited by this paper

• Synthesis and biological activity of vicinal diaryl-substituted 1H-imidazoles

  2007cites this paper
• Mechanistically Identified Suitable Biomarkers of Exposure, Effect, and Susceptibility for Silicosis and Coal-Worker'S Pneumoconiosis: A Comprehensive Review

  2006cites this paper
• Identification of a High-Affinity Receptor for Interleukin la and Interleukin 1# on Cultured Human Rheumatoid Synovial Cells

  2003cites this paper
• IDENTIFICATION OF A HIGH-AFFINITY RECEPTOR FOR NATIVE HUMAN INTERLEUKIN 10 AND INTERLEUKIN I a ON NORMAL HUMAN LUNG FIBROBLASTS

  2003cites this paper
• Generation of biologically active IL-1 beta by matrix metalloproteinases: a novel caspase-1-independent pathway of IL-1 beta processing.

  1998cites this paper
• Generation of Biologically Active IL-1β by Matrix Metalloproteinases: A Novel Caspase-1-Independent Pathway of IL-1β Processing

  1998cites this paper
• Role of Interleukin-1β Converting Enzyme (ICE) in Acute Myelogenous Leukemia Cell Proliferation and Programmed Cell Death

  1997cites this paper
• Interleukin-1: basic science and clinical applications.

  1997cites this paper
• Biologically active, alternatively processed interleukin‐1β in psoriatic scales

  1997cites this paper
• Structure and function of interleukin-1 beta converting enzyme.

  1997cites this paper
• Structure and Function of Interleukin-1β Converting Enzyme

  1997cites this paper
• Biologically active, alternatively processed interleukin-1 beta in psoriatic scales.

  1997cites this paper
• Role of interleukin-1 beta converting enzyme (ICE) in acute myelogenous leukemia cell proliferation and programmed cell death.

  1997cites this paper
• Tissue distribution of human gliostatin/platelet-derived endothelial cell growth factor (PD-ECGF) and its drug-induced expression.

  1996cites this paper
• IL-1 beta activation in human epidermis.

  1996cites this paper
• Resistance to fever induction and impaired acute-phase response in interleukin-1 beta-deficient mice.

  1995cites this paper
• 2,4,5- triarylimidazole inhibitors of IL-1 biosynthesis

  1995cites this paper
• Interleukin-1 and its inhibitors: implications for disease biology and therapy.

  1995cites this paper
• Leucocyte interleukin-1-like activity in the common seal (Phoca vitulina) and grey seal (Halichoerus grypus).

  1995cites this paper
• Peripheral blood and synovial fluid monocyte expression of interleukin 1 alpha and 1 beta during active rheumatoid arthritis.

  1995cites this paper
• Early circulating erythroid progenitors (BFU-E) in sickle cell anemia

  1993cites this paper
• Recombinant cytokines as immunological adjuvants

  1993cites this paper
• The IL-1 beta converting enzyme as a therapeutic target.

  1993cites this paper
• The IL‐1β Converting Enzyme as a Therapeutic Target

  1993cites this paper
• Enumeration of interleukin-1 alpha and beta producing cells by flow cytometry.

  1992cites this paper
• Cloning, expression and characterization of ovine interleukins 1α and β

  1992cites this paper
• Production, purification and characterization of canine prostromelysin.

  1992cites this paper
• Cytokines: from clone to clinic.

  1992cites this paper
• Further aspects of IL-1 beta secretion revealed by transfected monkey kidney cells.

  1992cites this paper
• Cloning, expression and characterization of ovine interleukins 1 alpha and beta.

  1992cites this paper
• Further aspects of IL-1β secretion revealed by transfected monkey kidney cells

  1992cites this paper
• Molecular cloning and characterization of ovine IL-1 alpha and IL-1 beta.

  1991cites this paper
• The Transforming Growth Factor-βs

  1991cites this paper
• IL-1-converting enzyme requires aspartic acid residues for processing of the IL-1 beta precursor at two distinct sites and does not cleave 31-kDa IL-1 alpha.

  1991cites this paper
• Processing of precursor interleukin 1 beta and inflammatory disease.

  1990cites this paper
• PRODUCTION AND PURIFICATION OF PROSTROMELYSIN AND PROCOLLAGENASE GINGIVAL FIBROBLASTS FROM IL-1 BETA-STIMULATED HUMAN

  1990cites this paper
• Comparison of Biological and Immunological Activities of Human Monocyte‐Derived Interleukin 1β and Human Recombinant Interleukin 1β

  1990cites this paper
• Structure and Function of Murine and Human IL-1 Receptors

  1990cites this paper
• Cytokines and endothelial cell biology.

  1990cites this paper
• Production and purification of prostromelysin and procollagenase from IL-1 beta-stimulated human gingival fibroblasts.

  1990cites this paper
• Identification of interleukin-1 in equine osteoarthritic joint effusions.

  1990cites this paper
• Comparison of biological and immunological activities of human monocyte-derived interleukin 1 beta and human recombinant interleukin 1 beta.

  1990cites this paper
• IL-1 binds to high affinity receptors on human osteosarcoma cells and potentiates prostaglandin E2 stimulation of cAMP production.

  1990cites this paper
• Identification of calcium-activated neutral protease as a processing enzyme of human interleukin 1 alpha.

  1990cites this paper
• Interleukin 1: past, present, future

  1989cites this paper
• Escherichia coli-derived human interferon-gamma with Cys-Tyr-Cys at the N-terminus is partially N alpha-acylated.

  1989cites this paper
• Purification to homogeneity and amino acid sequence analysis of a receptor protein for interleukin 1

  1989cites this paper
• Purification and characterization of tumor inhibitory factor-2: its identity to interleukin 1.

  1989cites this paper
• Purification and characterization of human recombinant precursor interleukin 1 beta.

  1989cites this paper
• Production of interleukin-1-alpha and -beta by human peripheral polymorphonuclear neutrophils.

  1989cites this paper
• Cloning of rabbit interleukin-1 beta: differential evolution of IL-1 alpha and IL-1 beta proteins.

  1989cites this paper
• Release of interleukin-1 beta associated with potent cytocidal action of staphylococcal alpha-toxin on human monocytes

  1989cites this paper
• Species preference of bovine thymocytes and fibroblasts for bovine interleukin 1.

  1989cites this paper
• Isolation and characterization of biologically active murine interleukin-1 alpha derived from expression of a synthetic gene in Escherichia coli.

  1989cites this paper
• Production and purification of bovine monocyte-derived interleukin 1.

  1989cites this paper
• Paraneoplastic syndrome of hypercalcemia and leukocytosis caused by squamous carcinoma cells (T3M-1) producing parathyroid hormone-related protein, interleukin 1 alpha, and granulocyte colony-stimulating factor.

  1989cites this paper
• Escherichia coli-derived human interferon-γ with CysTyrCys at the N-terminus is partially Nα-acylated

  1989cites this paper
• Production of interleukin 1 activity by cultured human melanoma cells.

  1989cites this paper
• Preliminary X-ray crystallography studies of recombinant human interleukin-1 alpha. Purification and structural characterization.

  1989cites this paper
• 4 – Recombinant Cytokines and their Therapeutic Value in Veterinary Medicine

  1989cites this paper
• Transforming growth factor beta: biochemistry and roles in embryogenesis, tissue repair and remodeling, and carcinogenesis.

  1988cites this paper
• Neutrophil chemoattractant and IL-1-like activity in samples from psoriatic skin lesions. Further characterization.

  1988cites this paper
• The production of myeloid blood cells and their regulation during health and disease.

  1988cites this paper
• Identification of different charged species of a human monocyte derived neutrophil activating peptide (MONAP).

  1988cites this paper
• Interleukin 1 beta is localized in the cytoplasmic ground substance but is largely absent from the Golgi apparatus and plasma membranes of stimulated human monocytes

  1988cites this paper
• Structure-activity studies of human IL-1 beta with mature and truncated proteins expressed in Escherichia coli.

  1988cites this paper
• The Role of Tumor Necrosis Factor and Interleukin 1 in the Immunoinflammatory Response

  1988cites this paper
• Identification of a high-affinity receptor for interleukin 1 alpha and interleukin 1 beta on cultured human rheumatoid synovial cells.

  1988cites this paper
• Prostaglandins, leukotrienes, phospholipase, platelet activating factor, and cytokines: an integrated approach to inflammation of human skin.

  1988cites this paper
• Induction of alpha 1-acid glycoprotein by recombinant human interleukin-1 in rat hepatoma cells.

  1988cites this paper
• Mapping of biologically relevant sites on human IL-1 beta using monoclonal antibodies.

  1988cites this paper
• Cloning, sequence and expression of bovine interleukin 1 alpha and interleukin 1 beta complementary DNAs.

  1988cites this paper
• Site-specific mutagenesis of the human interleukin-1 beta gene: structure-function analysis of the cysteine residues.

  1988cites this paper
• Site-specific mutagenesis of the human interleukin-1 beta gene: the role of arginine residue at the N-terminal region.

  1988cites this paper
• Production of interleukin-1 alpha and a parathyroid hormone-like factor by a squamous cell carcinoma of the esophagus (EC-GI) derived from a patient with hypercalcemia.

  1988cites this paper
• The close relationship between interleukin‐1 and fibroblast proliferating factor from peripheral mononuclear cells of patients with chronic hepatitis and liver cirrhosis

  1988cites this paper
• Cloning, sequence and expression of bovine interleukin 1α and interleukin 1β complementary DNAs

  1988cites this paper
• Sandwich enzyme immunoassay for human interleukin-1 beta (hIL-1 beta) in urine.

  1987cites this paper
• The interleukin-1 receptor.

  1987cites this paper
• Molecular Studies on Murine Interleukin 1

  1987cites this paper
• Determination of the minimum polypeptide lengths of the functionally active sites of human interleukins 1 alpha and 1 beta.

  1987cites this paper
• Molecular Modification of Lymphokines by in Vitro Mutagenesis

  1987cites this paper
• Purification and characterization of human recombinant interleukin-1 beta.

  1987cites this paper
• Localization of human mononuclear cell interleukin 1.

  1987cites this paper
• cDNA cloning of IL-1 alpha and IL-1 beta from mRNA of U937 cell line.

  1987cites this paper
• Expression in Escherichia coli of fully active recombinant human IL 1 beta: comparison with native human IL 1 beta.

  1987cites this paper
• Sandwich enzyme immunoassay for human interleukin-1β (hIL-1β) in urine

  1987cites this paper
• Tumor necrosis factor and interleukin 1: cytokines with multiple overlapping biological activities.

  1987cites this paper
• Purification and characterization of human interleukin-1 alpha produced in Escherichia coli.

  1987cites this paper
• Evaluation of alveolar macrophages in normals and individuals with active pulmonary sarcoidosis for the spontaneous expression of the interleukin-1 beta gene.

  1987cites this paper
• cDNA cloning of IL-1α and IL-1β from mRNA of U937 cell line

  1987cites this paper
• Purification and partial biochemical characterization of a human monocyte-derived, neutrophil-activating peptide that lacks interleukin 1 activity.

  1987cites this paper
• Preparation and characterization of polyclonal and monoclonal antibodies against human interleukin 1 alpha (IL 1 alpha).

  1987cites this paper
• Metabolism of protein anticancer agents.

  1987cites this paper
• Epstein-Barr virus-containing epithelial cells from nasopharyngeal carcinoma produce interleukin 1 alpha.

  1987cites this paper
• A preliminary crystallographic study of recombinant human interleukin 1 beta.

  1987cites this paper
• A Nude-Mouse-Grown NPC Tumor Produces Interleukin-1

  1987cites this paper
• Molecular Biology of Interleukin 1

  1987cites this paper
• Recombinant-derived interleukin-1 alpha stabilized against specific deamidation.

  1987cites this paper
• Identification of a high-affinity receptor for native human interleukin 1 beta and interleukin 1 alpha on normal human lung fibroblasts

  1987cites this paper