Identification of essential genes in cultured mammalian cells using small interfering RNAs.

No abstract is available for this paper.

• Publication year

  2001

• Venue

  Journal of Cell Science

• Publication date

  2001-12-15

• Fields of study

  Biology, Medicine

• Identifiers
  PMID 11792820
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Genome-wide RNAi.

  2001cited by this paper
• Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

  2001influential reference
• Broadcast interference - functional genomics.

  2001cited by this paper
• Lamins and apoptosis: a two-way street?

  2001cited by this paper
• Functional genomics: the worm scores a knockout.

  2001cited by this paper
• Regulation of actin filament network formation through ARP2/3 complex: activation by a diverse array of proteins.

  2001cited by this paper
• Lamins and Apoptosis

  2001cited by this paper
• RNA Interference and Small Interfering RNAs

  2001cited by this paper
• Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi.

  2001cited by this paper
• Gene silencing as an adaptive defence against viruses

  2001cited by this paper
• RNA interference--2001.

  2001cited by this paper
• RNA-based silencing strategies in plants.

  2001cited by this paper
• Post-transcriptional gene silencing by double-stranded RNA

  2001cited by this paper
• Role for a bidentate ribonuclease in the initiation step of RNA interference

  2001cited by this paper
• RNA interference is mediated by 21- and 22-nucleotide RNAs.

  2001influential reference
• Lamins and disease: insights into nuclear infrastructure.

  2001cited by this paper
• RNA interference. The short answer.

  2001cited by this paper
• Cell division: Mitotic kinases as regulators of cell division and its checkpoints

  2001influential reference
• Lamins in disease: why do ubiquitously expressed nuclear envelope proteins give rise to tissue-specific disease phenotypes?

  2001cited by this paper
• Involvement of the Lamin Rod Domain in Heterotypic Lamin Interactions Important for Nuclear Organization

  2001cited by this paper
• CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint

  2000cited by this paper
• The silence of the genes.

  2000cited by this paper
• Specific interference with gene function by double-stranded RNA in early mouse development

  2000cited by this paper
• GAS41, a Highly Conserved Protein in Eukaryotic Nuclei, Binds to NuMA*

  2000cited by this paper
• Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III

  2000cited by this paper
• Variation in the reversibility of evolution

  2000cited by this paper
• Functional genomic analysis of C. elegans chromosome I by systematic RNA interference

  2000cited by this paper
• Essential roles for Caenorhabditis elegans lamin gene in nuclear organization, cell cycle progression, and spatial organization of nuclear pore complexes.

  2000cited by this paper
• Double-stranded RNA as a template for gene silencing.

  2000cited by this paper
• Targeted deletion of keratins 18 and 19 leads to trophoblast fragility and early embryonic lethality

  2000cited by this paper
• CENP-E as an essential component of the mitotic checkpoint in vitro.

  2000cited by this paper
• Post‐transcriptional gene‐silencing: RNAs on the attack or on the defense?

  2000cited by this paper
• dsRNA-mediated gene silencing in cultured Drosophila cells: a tissue culture model for the analysis of RNA interference.

  2000cited by this paper
• Sensitive assay of RNA interference in Drosophila and Chinese hamster cultured cells using firefly luciferase gene as target

  2000cited by this paper
• Focal adhesions - the cytoskeletal connection.

  2000cited by this paper
• Post-transcriptional gene silencing across kingdoms.

  2000cited by this paper
• Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference.

  2000cited by this paper
• Loss of a-Type Lamin Expression Compromises Nuclear Envelope Integrity Leading to Muscular Dystrophy

  1999cited by this paper
• The vasodilator‐stimulated phosphoprotein (VASP) is involved in cGMP‐ and cAMP‐mediated inhibition of agonist‐induced platelet aggregation, but is dispensable for smooth muscle function

  1999cited by this paper
• Molecular Dissection of Zyxin Function Reveals Its Involvement in Cell Motility

  1999cited by this paper
• RNA-triggered gene silencing.

  1999cited by this paper
• The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.

  1999cited by this paper
• Guidebook to cytoskeletal and motor proteins

  1999cited by this paper
• Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen.

  1999cited by this paper
• Controlling your losses: conditional gene silencing in mammals.

  1998cited by this paper
• Vinculin knockout results in heart and brain defects during embryonic development.

  1998cited by this paper
• Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins.

  1995cited by this paper
• Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo.

  1995cited by this paper
• Mice lacking vimentin develop and reproduce without an obvious phenotype.

  1994cited by this paper
• Identification of a novel X-linked gene responsible for Emery-Dreifuss muscular dystrophy

  1994cited by this paper
• Analysis of simian virus 40 small t antigen-induced progression of rat F111 cells minimally transformed by large T antigen

  1993cited by this paper
• Differential timing of nuclear lamin A/C expression in the various organs of the mouse embryo and the young animal: a developmental study.

  1989cited by this paper

• Targets selection and field evaluation of an RNA biopesticide to control Phyllotreta striolata.

  2025cites this paper
• Drosophila as a Model for Studying the Roles of Lamins in Normal Tissues and Laminopathies

  2025cites this paper
• Proteins Lamin A and SUN1 are exclusively decreased in nuclear grade 4 clear cell renal cell carcinoma.

  2025cites this paper
• Elevation of the mechanically-sensitive protein emerin links nuclear mechanotransduction to tau-induced cytoskeletal remodeling in neurons

  2025cites this paper
• RNA interference-based therapeutics against aquatic viral diseases: Recent advances in fish and crustaceans (2010-2025).

  2025cites this paper
• The dual guardians of cellular stability: exploring nesprin and lamin in senescence

  2025cites this paper
• Nuclear destabilisation – a possible genesis of cancer?

  2025cites this paper
• Genomic stop codon scanning reveals quantitative principles of nonsense-mediated mRNA decay

  2025cites this paper
• Imaging of endogenous RNA in live cells using sequence-activated fluorescent RNA probes

  2024cites this paper
• Impairment of Intermediate Filament Expression Reveals Impact on Cell Functions Independent from Keratinocyte Transformation

  2024cites this paper
• RNA Interference Applied to Crustacean Aquaculture

  2024cites this paper
• Ultrasound-Mediated Lysozyme Microbubbles Targeting NOX4 Knockdown Alleviate Cisplatin-Exposed Cochlear Hair Cell Ototoxicity

  2024cites this paper
• The Role of non-muscle actin paralogs in cell cycle progression and proliferation.

  2023cites this paper
• Perturbed actin cap as a new personalized biomarker in primary fibroblasts of Huntington’s disease patients

  2023cites this paper
• RNAi-Based Therapy: Combating Shrimp Viral Diseases

  2023cites this paper
• Construction and application of the conditionally essential gene knockdown library in Klebsiella pneumoniae to screen potential antimicrobial targets and virulence genes via Mobile-CRISPRi-seq

  2023cites this paper
• Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells

  2022cites this paper
• Perturbed actin cap and nuclear morphology in primary fibroblasts of Huntington’s disease patients as a new phenotypic marker for personalized drug evaluation

  2022cites this paper
• A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents

  2022cites this paper
• Metformin is a Novel Suppressor for Vimentin in Human Gastric Cancer Cell Line

  2022cites this paper
• Lamin B1 is a potential therapeutic target and prognostic biomarker for hepatocellular carcinoma

  2022cites this paper
• Myocardial Cytoskeletal Adaptations in Advanced Kidney Disease

  2022cites this paper
• Structure and mechanics of the human Nuclear Pore Complex basket

  2022cites this paper
• Nuclear Lamins: Key Proteins for Embryonic Development

  2022cites this paper
• Genetic predisposition study of heart failure and its association with cardiomyopathy

  2022cites this paper
• Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity

  2022influential citation
• Progesterone depletion results in Lamin B1 loss and induction of cell death in mouse trophoblast giant cells

  2021cites this paper
• Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase

  2021cites this paper
• Scaling concepts in ‘omics: Nuclear lamin-B scales with tumor growth and often predicts poor prognosis, unlike fibrosis

  2021cites this paper
• Sequence-specific recognition of a coding segment of human DACH1 gene via short pyrimidine/purine oligonucleotides

  2021cites this paper
• A Novel Artificially Humanized Anti-Cripto-1 Antibody Suppressing Cancer Cell Growth

  2021cites this paper
• Scaling concepts in ’omics: nuclear lamin-B scales with tumor growth and predicts poor prognosis, whereas fibrosis can be pro-survival

  2021cites this paper
• Zyxin Is Involved in Fibroblast Rigidity Sensing and Durotaxis

  2021cites this paper
• The nuclear lamina binds the EBV genome during latency and regulates viral gene expression

  2021cites this paper
• DEC1 negatively regulates CYP2B6 expression by binding to the CYP2B6 promoter region ascribed to IL-6-induced downregulation of CYP2B6 expression in HeLa cells

  2021cites this paper
• The Effects of Single Nucleotide Polymorphisms in Cancer RNAi Therapies

  2020cites this paper
• Prediction of Essential Genes in Comparison States Using Machine Learning

  2020cites this paper
• BUBR1 Pseudokinase Domain Promotes Kinetochore PP2A-B56 Recruitment, Spindle Checkpoint Silencing, and Chromosome Alignment.

  2020cites this paper
• Neuron type‐specific increase in lamin B1 contributes to nuclear dysfunction in Huntington’s disease

  2020cites this paper
• Nucleoporin TPR is an integral component of the TREX-2 mRNA export pathway

  2020cites this paper
• Cancer biology functional genomics: From small RNAs to big dreams

  2020cites this paper
• Predicting essential genes of 41 prokaryotes by a semi-supervised method.

  2020cites this paper
• Lamin A/C Mechanotransduction in Laminopathies

  2020cites this paper
• Lamin B1 deficiency promotes malignancy and predicts poor prognosis in gastric cancer.

  2020cites this paper
• Cell models of tight junction biology

  2020cites this paper
• Intelligent Classification and Analysis of Essential Genes Using Quantitative Methods

  2020cites this paper
• Keratins couple with the nuclear lamina and regulate proliferation in colonic epithelial cells

  2020cites this paper
• Nuclear filaments: role in chromosomal positioning and gene expression

  2020cites this paper
• An unbiased, quantitative and versatile method for determining misaligned and lagging chromosome during mitosis

  2020cites this paper
• Neuron-specific increase in lamin B1 disrupts nuclear function in Huntington’s disease

  2020cites this paper
• Yeast as a Model to Understand Actin-Mediated Cellular Functions in Mammals—Illustrated with Four Actin Cytoskeleton Proteins

  2020cites this paper
• Applications of Functional Genomics for Drug Discovery

  2020cites this paper
• Synthetic mammalian signaling circuits for robust cell population control

  2020cites this paper
• A quantitative and semiautomated method for determining misaligned and lagging chromosomes during mitosis

  2020cites this paper
• PLCG2 promotes hepatocyte proliferation in vitro via NF-κB and ERK pathway by targeting bcl2, myc and ccnd1

  2019cites this paper
• Lamin Protein Gene Expression in Childhood Acute Lymphoblastic Leukemia

  2019cites this paper
• An Emerin LEM-Domain Mutation Impairs Cell Response to Mechanical Stress

  2019cites this paper
• Allele-specific silencing as treatment for gene duplication disorders: proof-of-principle in autosomal dominant leukodystrophy.

  2019cites this paper
• Distinct Basket Nucleoporins roles in Nuclear Pore Function and Gene Expression: Tpr is an integral component of the TREX-2 mRNA export pathway

  2019cites this paper
• 1 An emerin LEM-domain mutation impairs cell 2 response to mechanical stress 3

  2019cites this paper
• Postnatal Development of Mice with Combined Genetic Depletions of Lamin A/C, Emerin and Lamina-associated Polypeptide 1.

  2019cites this paper
• A Deep Learning Framework for Identifying Essential Proteins by Integrating Multiple Types of Biological Information

  2019cites this paper
• Combinatorial siRNA Polyplexes for Receptor Targeting.

  2019cites this paper
• In-vitro inhibition of spring viremia of carp virus replication by RNA interference targeting the RNA-dependent RNA polymerase gene.

  2019cites this paper
• Cellular and Animal Models of Striated Muscle Laminopathies

  2019cites this paper
• The essentiality of drug targets: an analysis of current literature and genomic databases.

  2019cites this paper
• An absence of lamin B1 in migrating neurons causes nuclear membrane ruptures and cell death

  2019cites this paper
• Host-Induced Gene Silencing of MoAP1 Confers Broad-Spectrum Resistance to Magnaporthe oryzae

  2019cites this paper
• A siRNA‐based method for efficient silencing of PYROXD1 gene expression in the colon cancer cell line HCT116

  2019cites this paper
• Targeted regulationof STAT3 by miR-29a in mediating Taxol resistance of nasopharyngeal carcinoma cell line CNE-1

  2018cites this paper
• Nucleoporin 153 regulates estrogen-dependent nuclear translocation of endothelial nitric oxide synthase and estrogen receptor beta in prostate cancer

  2018cites this paper
• GAS41 Recognizes Diacetylated Histone H3 through a Bivalent Binding Mode.

  2018cites this paper
• [Searching for essential genes in cancer genomes].

  2018cites this paper
• Membrane Proteome of Invasive Retinoblastoma: Differential Proteins and Biomarkers

  2018cites this paper
• A novel mouse model carrying a human cytoplasmic dynein mutation shows motor behavior deficits consistent with Charcot-Marie-Tooth type 2O disease

  2018cites this paper
• Prevention of Pancreatic Cancer

  2018cites this paper
• Huaier suppresses proliferative and metastatic potential of prostate cancer PC3 cells via downregulation of Lamin B1 and induction of autophagy.

  2018cites this paper
• Fibroblasts lacking nuclear lamins do not have nuclear blebs or protrusions but nevertheless have frequent nuclear membrane ruptures

  2018cites this paper
• Mitochondrial aconitase is a key regulator of energy production for growth and protein expression in Chinese hamster ovary cells

  2018cites this paper
• Non-senescent Hydra tolerates severe disturbances in the nuclear lamina

  2018cites this paper
• Novel Human Polyomavirus Noncoding Control Regions Differ in Bidirectional Gene Expression according to Host Cell, Large T-Antigen Expression, and Clinically Occurring Rearrangements

  2018cites this paper
• Proteomic profiling of epileptogenesis in a rat model: Focus on cell stress, extracellular matrix and angiogenesis.

  2018cites this paper
• Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells

  2018cites this paper
• The G Protein-Coupled Receptor RAI3 Is an Independent Prognostic Factor for Pancreatic Cancer Survival and Regulates Proliferation via STAT3 Phosphorylation

  2017cites this paper
• Current insights into LMNA cardiomyopathies: Existing models and missing LINCs

  2017cites this paper
• Using a hydrogen-bond index to predict the gene-silencing efficiency of siRNA based on the local structure of mRNA

  2017cites this paper
• Are non-muscle actin isoforms functionally equivalent?

  2017cites this paper
• 9 Prevention of Pancreatic Cancer

  2017cites this paper
• Soc . Physiol . Sci . 37 ( 2 ) , 165-175 Protective Effect of Copper ( I )-Nicotinate Complex on DAB-Induced Hepatocellular Carcinoma in Rats

  2017cites this paper
• The lamin code

  2017cites this paper
• Identification and characterization of protein-protein interactions in the nuclear envelope

  2017cites this paper
• Interference of central metabolism (TCA cycle) to influence CHO cell productivity

  2017cites this paper
• The role of β-catenin in the initiation and metastasis of TA2 mice spontaneous breast cancer

  2017cites this paper
• Delivery of ENaC siRNA to epithelial cells mediated by a targeted nanocomplex: a therapeutic strategy for cystic fibrosis

  2017cites this paper
• Involvement of β- and γ-actin isoforms in actin cytoskeleton organization and migration abilities of bleb-forming human colon cancer cells

  2017cites this paper
• A mesoporous silica composite scaffold: Cell behaviors, biomineralization and mechanical properties

  2017cites this paper
• Essential Genes And Their Role In Autism Spectrum Disorder

  2017cites this paper
• Prediction of essential proteins based on subcellular localization and gene expression correlation

  2017cites this paper
• Regulation of Cell Proliferation and Migration by miR-203 via GAS41/miR-10b Axis in Human Glioblastoma Cells

  2016cites this paper
• In vivo, Ex Vivo, and In Vitro Approaches to Study Intermediate Filaments in the Eye Lens.

  2016cites this paper