Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase–Producing Escherichia coli ST410 in the United Kingdom

We characterized extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) in 32 Escherichia coli extended spectrum cephalosporin (ESC)-resistant clinical isolates from UK companion animals from several clinics. In addition, to investigate the possible dissemination of ESBL clinical isolates within a veterinary hospital, two ESBL-producing E. coli isolates from a dog with septic peritonitis and a cluster of environmental ESC-resistant E. coli isolates obtained from the same clinic and during the same time period, as these two particular ESBL-positive clinical isolates, were also included in the study. Molecular characterization identified blaCTX-M to be the most prevalent gene in ESC-resistant isolates, where 66% and 27% of clinical isolates carried blaCTX-M-15 and blaCTX-M-14, respectively. The only PMQR gene detected was aac(6')-Ib-cr, being found in 34% of the ESC E. coli isolates and was associated with the carriage of blaCTX-M-15. The clinical and environmental isolates investigated for hospital dissemination had a common ESBL/AmpC phenotype, carried blaCTX-M-15, and co-harbored blaOXA-1, blaTEM-1, blaCMY-2, and aac(6')-Ib-cr. Multilocus sequence typing identified them all as ST410, while pulse-field gel electrophoresis demonstrated 100% homology of clinical and environmental isolates, suggesting hospital environmental dissemination of CTX-M-15–producing E. coli ST410.

• Publication year

  2016

• Venue

  Microbial Drug Resistance

• Publication date

  2016-10-01

• Fields of study

  Medicine, Environmental Science

• Identifiers
  DOI 10.1089/mdr.2016.0036PMID 27314838PMCID 5073239
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Multilocus sequence typing and pulse-field gel electrophoresis linked the matched isolates to ST410 and showed complete relatedness, supporting veterinary hospital dissemination.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review
• The matched clinical and environmental isolates shared an ESBL/AmpC phenotype and co-harbored blaCTX-M-15, blaOXA-1, blaTEM-1, blaCMY-2, and aac(6')-Ib-cr.

  Confidence 0.96

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review
• aac(6')-Ib-cr was the only PMQR gene detected, appeared in 34% of the ESC-resistant E. coli isolates, and was associated with blaCTX-M-15 carriage.

  Confidence 0.97

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review
• blaCTX-M-15 was the most prevalent ESBL gene among the ESC-resistant clinical isolates, while blaCTX-M-14 was also common at a lower frequency.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review

• aac(6')-ib-cr

  resistance genes

  A plasmid-mediated quinolone resistance gene encoding an enzyme variant associated with reduced susceptibility to certain quinolones.

  Aliases: aac(6')-Ib-cr gene

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review
• blactx-m-14

  resistance genes

  Another extended-spectrum beta-lactamase gene variant detected in the E. coli isolates.

  Aliases: CTX-M-14, CTX-M-14 beta-lactamase gene

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review
• blactx-m-15

  resistance genes

  An extended-spectrum beta-lactamase gene variant detected in the E. coli isolates.

  Aliases: CTX-M-15, CTX-M-15 beta-lactamase gene

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review
• esbl/ampc phenotype

  phenotypes

  A combined beta-lactam resistance phenotype indicating extended-spectrum beta-lactamase and AmpC activity in an isolate.

  Aliases: ESBL and AmpC phenotype, ESBL/AmpC resistance phenotype

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review
• esc-resistant clinical isolates

  isolate collections

  Clinical Escherichia coli isolates from companion animals that were resistant to extended-spectrum cephalosporins and included in the resistance survey.

  Aliases: extended-spectrum cephalosporin-resistant clinical isolates, ESC-resistant E. coli isolates

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review
• pulse-field gel electrophoresis

  typing methods

  A DNA fingerprinting method used to compare genetic relatedness among bacterial isolates.

  Aliases: PFGE

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review
• st410

  sequence types

  A multilocus sequence typing lineage used to assign related E. coli isolates to sequence type 410.

  Aliases: sequence type 410

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review
• veterinary hospital dissemination

  epidemiologic phenomena

  Spread of resistant bacterial isolates within a veterinary hospital setting.

  Aliases: hospital dissemination, environmental dissemination within the veterinary hospital

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review

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