A Role for CREB Binding Protein and p300 Transcriptional Coactivators in Ets-1 Transactivation Functions

ABSTRACT The Ets-1 transcription factor plays a critical role in cell growth and development, but the means by which it activates transcription are still unclear (J. C. Bories, D. M. Willerford, D. Grevin, L. Davidson, A. Camus, P. Martin, D. Stehelin, F. W. Alt, and J. C. Borles, Nature 377:635–638, 1995; N. Muthusamy, K. Barton, and J. M. Leiden, Nature 377:639–642, 1995). Here we show that Ets-1 binds the transcriptional coactivators CREB binding protein (CBP) and the related p300 protein (together referred to as CBP/p300) and that this interaction is required for specific Ets-1 transactivation functions. The Ets-1- and c-Myb-dependent aminopeptidase N (CD13/APN) promoter and an Ets-1-dependent artificial promoter were repressed by adenovirus E1A, a CBP/p300-specific inhibitor. Furthermore, Ets-1 activity was potentiated by CBP and p300 overexpression. The transactivation function of Ets-1 correlated with its ability to bind an N-terminal cysteine- and histidine-rich region spanning CBP residues 313 to 452. Ets-1 also bound a second cysteine- and histidine-rich region of CBP, between residues 1449 and 1892. Both Ets-1 and CBP/p300 formed a stable immunoprecipitable nuclear complex, independent of DNA binding. This Ets-1–CBP/p300 immunocomplex possessed histone acetyltransferase activity, consistent with previous findings that CBP/p300 is associated with such enzyme activity. Our results indicate that CBP/p300 may mediate antagonistic and synergistic interactions between Ets-1 and other transcription factors that use CBP/p300 as a coactivator, including c-Myb and AP-1.

• Publication year

  1998

• Venue

  Molecular and Cellular Biology

• Publication date

  1998-04-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1128/MCB.18.4.2218PMID 9528793PMCID PMC121466
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Conserved mechanisms of Ras regulation of evolutionary related transcription factors, Ets1 and Pointed P2

  1997cited by this paper
• Synergistic activation of transcription by CBP and p53

  1997cited by this paper
• CREB-binding protein/p300 are transcriptional coactivators of p65.

  1997cited by this paper
• Nuclear integration of JAK/STAT and Ras/AP-1 signaling by CBP and p300.

  1997cited by this paper
• Recruitment of p300/CBP in p53-dependent signal pathways.

  1997cited by this paper
• RNA helicase A mediates association of CBP with RNA polymerase II.

  1997influential reference
• MafB is an interaction partner and repressor of Ets-1 that inhibits erythroid differentiation.

  1996cited by this paper
• The SV40 large T antigen and adenovirus E1a oncoproteins interact with distinct isoforms of the transcriptional co‐activator, p300.

  1996cited by this paper
• Transcription. A growing coactivator network.

  1996cited by this paper
• Regulation of the c-fos promoter by the ternary complex factor Sap-1a and its coactivator CBP.

  1996influential reference
• CREB-binding Protein Activates Transcription through Multiple Domains*

  1996cited by this paper
• Interaction of the co‐activator CBP with Myb proteins: effects on Myb‐specific transactivation and on the cooperativity with NF‐M.

  1996cited by this paper
• A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A

  1996cited by this paper
• The translocation t(8;16)(p11;p13) of acute myeloid leukaemia fuses a putative acetyltransferase to the CREB–binding protein

  1996influential reference
• Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation.

  1996influential reference
• Synergistic Transcriptional Activation of the Tissue Inhibitor of Metalloproteinases-1 Promoter via Functional Interaction of AP-1 and Ets-1 Transcription Factors (*)

  1996cited by this paper
• A growing coactivator network

  1996cited by this paper
• Role of CBP/P300 in nuclear receptor signalling

  1996cited by this paper
• The CBP co-activator is a histone acetyltransferase

  1996influential reference
• The transcriptional coactivators p300 and CBP are histone acetyltransferases.

  1996cited by this paper
• Myb-Ets fusion oncoprotein inhibits thyroid hormone receptor/c-ErbA and retinoic acid receptor functions: a novel mechanism of action for leukemogenic transformation by E26 avian retrovirus

  1996cited by this paper
• The adenovirus E1A-associated 300 kDa adaptor protein counteracts the inhibition of the collagenase promoter by E1A and represses transformation.

  1996cited by this paper
• GHF-1/Pit-1 Functions as a Cell-specific Integrator of Ras Signaling by Targeting the Ras Pathway to a Composite Ets-1/GHF-1 Response Element*

  1996cited by this paper
• Adaptor-mediated Recruitment of RNA Polymerase II to a Signal-dependent Activator (*)

  1996cited by this paper
• ZZ and TAZ: new putative zinc fingers in dystrophin and other proteins.

  1996influential reference
• The signal-dependent coactivator CBP is a nuclear target for pp90RSK.

  1996cited by this paper
• CBP as a transcriptional coactivator of c-Myb.

  1996cited by this paper
• Phosphorylation of CREB at Ser-133 induces complex formation with CREB-binding protein via a direct mechanism

  1996influential reference
• A CBP integrator complex mediates transcriptional activation and AP-1 inhibition by nuclear receptors.

  1996influential reference
• Adenovirus E1A downregulates cJun- and JunB-mediated transcription by targeting their coactivator p300

  1996cited by this paper
• Ras-mediated phosphorylation of a conserved threonine residue enhances the transactivation activities of c-Ets1 and c-Ets2

  1996influential reference
• A family of transcriptional adaptor proteins targeted by the E1A oncoprotein

  1995cited by this paper
• Increased T-cell apoptosis and terminal B-cell differentiation induced by inactivation of the Ets-1 proto-oncogene

  1995cited by this paper
• Adenoviral ElA-associated protein p300 as a functional homologue of the transcriptional co-activator CBP

  1995cited by this paper
• Rubinstein-Taybi syndrome caused by mutations in the transcriptional co-activator CBP

  1995cited by this paper
• Defective activation and survival of T cells lacking the Ets-1 transcription factor

  1995cited by this paper
• Myb and Ets Proteins Cooperate to Transactivate an Early Myeloid Gene (*)

  1995cited by this paper
• CBP‐induced stimulation of c‐Fos activity is abrogated by E1A.

  1995cited by this paper
• Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: a molecular model for the formation of heterochromatin in yeast.

  1995cited by this paper
• JUN cooperates with the ETS domain protein pointed to induce photoreceptor R7 fate in the Drosophila eye.

  1995cited by this paper
• An Inactivating Point Mutation Demonstrates That Interaction of cAMP Response Element Binding Protein (CREB) with the CREB Binding Protein Is Not Sufficient for Transcriptional Activation (*)

  1995cited by this paper
• Functional interaction of c-Ets-1 and GHF-1/Pit-1 mediates Ras activation of pituitary-specific gene expression: mapping of the essential c-Ets-1 domain

  1995cited by this paper
• Finding prospective partners in the library: the two-hybrid system and phage display find a match.

  1995cited by this paper
• Multiple protein kinase A-regulated events are required for transcriptional induction by cAMP.

  1995cited by this paper
• cAMP Response Element-binding Protein (CREB) Interacts with Transcription Factors IIB and IID (*)

  1995cited by this paper
• Participation of Ets transcription factors in the glucocorticoid response of the rat tyrosine aminotransferase gene

  1994cited by this paper
• Activation of cAMP and mitogen responsive genes relies on a common nuclear factor

  1994cited by this paper
• The activities of two Ets-related transcription factors required for Drosophila eye development are modulated by the Ras/MAPK pathway.

  1994cited by this paper
• Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.

  1994cited by this paper
• The ETS domain protein Pointed-P2 is a target of MAP kinase in the Sevenless signal transduction pathway

  1994cited by this paper
• Ets-2 and c-Myb act independently in regulating expression of the hematopoietic stem cell antigen CD34.

  1994cited by this paper
• A model for gene evolution of the ets-1/ets-2 transcription factors based on structural and functional homologies.

  1994cited by this paper
• Nuclear protein CBP is a coactivator for the transcription factor CREB

  1994influential reference
• E1A-associated p300 and CREB-associated CBP belong to a conserved family of coactivators.

  1994cited by this paper
• The cAMP-regulated transcription factor CREB interacts with a component of the TFIID complex.

  1994cited by this paper
• Opposing actions of c-ets/PU.1 and c-myb protooncogene products in regulating the macrophage-specific promoters of the human and mouse colony-stimulating factor-1 receptor (c-fms) genes

  1994cited by this paper
• p300, and p300-associated proteins, are components of TATA-binding protein (TBP) complexes.

  1993cited by this paper
• Synergism in transcriptional activation: a kinetic view.

  1993cited by this paper
• The Drosophila gene pointed encodes two ETS-like proteins which are involved in the development of the midline glial cells.

  1993cited by this paper
• Influence of the v‐Myb transactivation domain on the oncoprotein's transformation specificity.

  1993cited by this paper
• Protein-kinase-A-dependent activator in transcription factor CREB reveals new role for CREM repressers

  1993cited by this paper
• Distinct activation domains within cAMP response element-binding protein (CREB) mediate basal and cAMP-stimulated transcription.

  1993cited by this paper
• The Ets family of transcription factors.

  1993influential reference
• Regulation of lymphoid-specific immunoglobulin mu heavy chain gene enhancer by ETS-domain proteins.

  1993cited by this paper
• Protein-kinase-A-dependent activator in transcription factor CREB reveals new role for CREM repressors.

  1993cited by this paper
• Phylogeny of the p68c-ets-1 amino-terminal transactivating domain reveals some highly conserved structural features.

  1992cited by this paper
• Myb and Ets proteins cooperate in transcriptional activation of the mim-1 promoter.

  1992cited by this paper
• The ets gene family.

  1992cited by this paper
• Myb: a transcriptional activator linking proliferation and differentiation in hematopoietic cells.

  1992cited by this paper
• GAL4 fusion vectors for expression in yeast or mammalian cells.

  1992cited by this paper
• Ethidium bromide provides a simple tool for identifying genuine DNA-independent protein associations.

  1992cited by this paper
• Fusion of the nuclear oncoproteins v-Myb and v-Ets is required for the leukemogenicity of E26 virus.

  1991cited by this paper
• v-myb and v-ets transform chicken erythroid cells and cooperate both in trans and in cis to induce distinct differentiation phenotypes.

  1991cited by this paper
• The c-ets proto-oncogenes encode transcription factors that cooperate with c-Fos and c-Jun for transcriptional activation

  1990influential reference
• Analysis of E1A-mediated growth regulation functions: binding of the 300-kilodalton cellular product correlates with E1A enhancer repression function and DNA synthesis-inducing activity

  1990cited by this paper
• Evidence for interaction of different eukaryotic transcriptional activators with distinct cellular targets

  1990cited by this paper
• Site-directed mutagenesis by overlap extension using the polymerase chain reaction.

  1989cited by this paper
• Rapid detection of octamer binding proteins with 'mini-extracts', prepared from a small number of cells.

  1989cited by this paper
• Mammalian ets-1 and ets-2 genes encode highly conserved proteins.

  1988cited by this paper
• A putative second cell-derived oncogene of the avian leukaemia retrovirus E26

  1983cited by this paper
• Tripartite structure of the avian erythroblastosis virus E26 transforming gene

  1983cited by this paper

• TET1 non-catalytic activity shapes the chromatin landscape that directs de novo methylation establishment in the male germline

  2025cites this paper
• Ets-1 Protein Interactors in Transcriptional Activity Regulation

  2025cites this paper
• ETS1 promotes the expression of Ctsb and Mmp13 during the differentiation of septoclasts from pericytes

  2025cites this paper
• Lineage- and stage-specific activity of antigen receptor gene enhancers during lymphocyte development

  2024cites this paper
• M2 macrophages independently promote beige adipogenesis via blocking adipocyte Ets1

  2024cites this paper
• ETS1 Function in Leukemia and Lymphoma.

  2024cites this paper
• Batf stabilizes Th17 cell development via impaired Stat5 recruitment of Ets1‐Runx1 complexes

  2023cites this paper
• CREB-mediated transcriptional activation of NRMT1 drives muscle differentiation

  2021cites this paper
• Glycogen synthase kinase-3 beta (GSK3β)-mediated phosphorylation of ETS1 promotes progression of ovarian carcinoma

  2021cites this paper
• Interplay between cofactors and transcription factors in hematopoiesis and hematological malignancies

  2021cites this paper
• The pro-angiogenesis effect of miR33a-5p/Ets-1/DKK1 signaling in ox-LDL induced HUVECs

  2021cites this paper
• Transcriptional and signalling regulation of skin epithelial stem cells in homeostasis, wounds and cancer

  2020cites this paper
• TCF21 and AP-1 interact through epigenetic modifications to regulate coronary artery disease gene expression

  2019cites this paper
• ETS1 is coexpressed with ZEB2 and mediates ZEB2‐induced epithelial‐mesenchymal transition in human tumors

  2019cites this paper
• Ets1-Mediated Acetylation of FoxO1 Is Critical for Gluconeogenesis Regulation during Feed-Fast Cycles.

  2019cites this paper
• Transcription Factor Elf3 Modulates Vasopressin-Induced Aquaporin-2 Gene Expression in Kidney Collecting Duct Cells

  2019cites this paper
• Ets-1 enhances tumor migration through regulation of CCR7 expression

  2019cites this paper
• This information is current as Binding with ETS 1 RUNX 1 Function from Its Cooperative : Uncoupling Tcrb Activation of Mouse Majumder

  2017cites this paper
• Priming of transcriptional memory responses via the chromatin accessibility landscape in T cells

  2017cites this paper
• Activation of Mouse Tcrb: Uncoupling RUNX1 Function from Its Cooperative Binding with ETS1

  2017cites this paper
• ETS-targeted therapy: can it substitute for MEK inhibitors?

  2017cites this paper
• ELF3 modulates type II collagen gene (COL2A1) transcription in chondrocytes by inhibiting SOX9-CBP/p300-driven histone acetyltransferase activity

  2017cites this paper
• Grainyhead-like 2 inhibits the coactivator p300, suppressing tubulogenesis and the epithelial–mesenchymal transition

  2016cites this paper
• Addiction of lung cancer cells to GOF p53 is promoted by up-regulation of epidermal growth factor receptor through multiple contacts with p53 transactivation domain and promoter

  2016cites this paper
• Grainyhead-like 2 Reverses the Metabolic Changes Induced by the Oncogenic Epithelial–Mesenchymal Transition: Effects on Anoikis

  2016cites this paper
• Mapping the response of human fibroblast growth factor 21 (FGF21) promoter to serum availability and lipoic acid in HepG2 hepatoma cells.

  2016cites this paper
• The role of the transcription factor Ets1 in carcinoma.

  2015cites this paper
• EGFR inhibition evokes innate drug resistance in lung cancer cells by preventing Akt activity and thus inactivating Ets-1 function

  2015cites this paper
• EGFR inhibition generates drug-tolerant persister cells by blocking AKT activity

  2015cites this paper
• The regulation and role of MS1 in the cardiac stress response

  2015cites this paper
• The transcription factor ETS1 in lymphomas: friend or foe?

  2015cites this paper
• The Proto-oncogene Transcription Factor Ets1 Regulates Neural Crest Development through Histone Deacetylase 1 to Mediate Output of Bone Morphogenetic Protein Signaling*

  2015cites this paper
• Atlas of Genetics and Cytogenetics in Oncology and Haematology

  2014cites this paper
• Histone acetyltransferase-deficient p300 mutants in diffuse large B cell lymphoma have altered transcriptional regulatory activities and are required for optimal cell growth

  2014cites this paper
• CBP/p300 Recruitment Signaling Activates Ets-1 and Ets-2 by Ras/Mitogen-Activated Protein Kinase

  2014cites this paper
• Crafting the Brain – Role of Histone Acetyltransferases in Neural Development and Disease

  2014cites this paper
• Situational Awareness: Regulation of the Myb Transcription Factor in Differentiation, the Cell Cycle and Oncogenesis

  2014cites this paper
• of Endothelial Cells Sarcoma-Associated Herpesvirus Infection VEGFR3 during Latent Kaposi's Ets-1 Is Required for the Activation of

  2014cites this paper
• Transcription Factor Ets1 Regulates Expression of Thioredoxin-Interacting Protein and Inhibits Insulin Secretion in Pancreatic β-Cells

  2014cites this paper
• highly essential collectively but not individually in peripheral B cells Global transcriptional coactivators CREB-binding protein and p300 are

  2013cites this paper
• Ets-1 Is Required for the Activation of VEGFR3 during Latent Kaposi's Sarcoma-Associated Herpesvirus Infection of Endothelial Cells

  2013cites this paper
• Latent Kaposi's Sarcoma Herpesvirus regulates host factors to induce Lymphangiogenesis and Angiogenesis

  2013cites this paper
• Regulation of HIV-1 latency by basal transcription

  2013cites this paper
• Identification de nouveaux partenaires protéiques de l'oncoprotéine Ets-1 et étude de sa régulation par l'enzyme de réparation de l'ADN PARP-1 au sein des cellules tumorales

  2013cites this paper
• IL-10 Expression in Th1 Cells Interaction of Ets-1 with HDAC1 Represses

  2012cites this paper
• Parathyroid Hormone–Related Peptide Signaling in Cancer

  2012cites this paper
• c-Ets1 inhibits the interaction of NF-κB and CREB, and downregulates IL-1β-induced MUC5AC overproduction during airway inflammation

  2012cites this paper
• Interaction of Ets-1 with HDAC1 Represses IL-10 Expression in Th1 Cells

  2012cites this paper
• Differential Transcription Factor Use by the KIR2DL4 Promoter under Constitutive and IL-2/15–Treated Conditions

  2012cites this paper
• KDM3B Is the H3K9 Demethylase Involved in Transcriptional Activation of lmo2 in Leukemia

  2012cites this paper
• Mechanisms elevating ORMDL3 expression in recurrent wheeze patients: role of Ets-1, p300 and CREB.

  2012cites this paper
• Protein kinase A regulates caspase-1 via Ets-1 in bone stromal cell-derived lesions: a link between cyclic AMP and pro-inflammatory pathways in osteoblast progenitors.

  2011cites this paper
• ETS1 (v-ets erythroblastosis virus E26 oncogene homolog 1 (avian))

  2011cites this paper
• Nicotinic α5 receptor subunit mRNA expression is associated with distant 5′ upstream polymorphisms

  2011cites this paper
• Regulation of endothelial cell development by ETS transcription factors.

  2011cites this paper
• Mutant K-Ras increases GSK-3β gene expression via an ETS-p300 transcriptional complex in pancreatic cancer

  2011cites this paper
• CITED2 controls the hypoxic signaling by snatching p300 from the two distinct activation domains of HIF-1α.

  2011influential citation
• Genome-wide promoter analysis of histone modifications in human monocyte-derived antigen presenting cells

  2010cites this paper
• Atlas of Genetics and Cytogenetics in Oncology and Haematology

  2010cites this paper
• The Ets-1 transcription factor controls the development and function of natural regulatory T cells

  2010cites this paper
• Protease activated receptor-1 inhibits the Maspin tumor-suppressor gene to determine the melanoma metastatic phenotype

  2010cites this paper
• Étude de la régulation transcriptionnelle de ICAM-1 : implication de la voie JAK/STAT

  2010cites this paper
• Ordered transcriptional factor recruitment and epigenetic regulation of tnf-α in necrotizing acute pancreatitis

  2010cites this paper
• Ras signaling requires dynamic properties of Ets1 for phosphorylation-enhanced binding to coactivator CBP

  2010cites this paper
• Blocage de maturation thymique et aberration des recombinaisons V(D)J : modèle des Leucémies Aigües Lymphoblastiques de la lignée T exprimant les onco-protéines à homéodomaines TLX1 et TLX3

  2010cites this paper
• DNA Specificity Determinants Associate with Distinct Transcription Factor Functions

  2009cites this paper
• Molecular control of HIV-1 postintegration latency: implications for the development of new therapeutic strategies

  2009influential citation
• Up-regulation of Bcl-xl by hepatocyte growth factor in human mesothelioma cells involves ETS transcription factors.

  2009cites this paper
• Transcriptional regulation of the human growth hormone receptor (hGHR) gene V2 promoter by transcriptional activators and repressor.

  2009cites this paper
• Regulation of natriuretic peptide receptor-A gene expression and stimulation of its guanylate cyclase activity by transcription factor Ets-1.

  2009cites this paper
• Functional cooperation between Stat-1 and ets-1 to optimize icam-1 gene transcription.

  2009cites this paper
• Thirty‐kilodalton Tat‐interacting protein suppresses tumor metastasis by inhibition of osteopontin transcription in human hepatocellular carcinoma

  2008cites this paper
• Interleukin-4 Suppresses Interleukin-1-Induced Expression of Matrix Metalloproteinase-3 in Human Gingival Fibroblasts

  2008cites this paper
• Activation of the VEGFR1 Chromatin Domain

  2008cites this paper
• The c-myc promoter: still MysterY and challenge.

  2008cites this paper
• Ets-2 and p160 proteins collaborate to regulate c-Myc in endocrine resistant breast cancer

  2008cites this paper
• cAMP-responding Element-binding Protein and c-Ets1 Interact in the Regulation of ATP-dependent MUC5AC Gene Expression*

  2008cites this paper
• transcriptional control by developmental cell signaling Three habits of highly effective signaling pathways: principles of

  2007cites this paper
• Differential utilization of transcription activation subdomains by distinct coactivators regulates Pit-1 basal and Ras responsiveness.

  2007cites this paper
• PTHrP P3 promoter activity in breast cancer cell lines: role of Ets1 and CBP (CREB binding protein).

  2007cites this paper
• Individual CREB‐target genes dictate usage of distinct cAMP‐responsive coactivation mechanisms

  2007cites this paper
• Regulation by Glycogen Synthase Kinase-3 Beta of CBP transcriptional coactivator involved in insulin-dependent glucagon gene transcription

  2007cites this paper
• Foxs and Ets in the transcriptional regulation of endothelial cell differentiation and angiogenesis.

  2007cites this paper
• DNA motifs in human and mouse proximal promoters predict tissue-specific expression.

  2006cites this paper
• Epigenetic Regulation of Manganese Superoxide Dismutase Expression in Human Breast Cancer Cells

  2006cites this paper
• Transcriptional regulation of guanylyl cyclase/natriuretic peptide receptor-A gene.

  2006cites this paper
• Attenuation of cardiac dysfunction by a PPAR-alpha agonist is associated with down-regulation of redox-regulated transcription factors.

  2006cites this paper
• Tissue-Specific Chromatin Modifications at a Multigene Locus Generate Asymmetric Transcriptional Interactions

  2006cites this paper
• Global transcriptional coactivators CREB-binding protein and p300 are highly essential collectively but not individually in peripheral B cells.

  2006cites this paper
• Redox-dependent Matrix Metalloproteinase-1 Expression Is Regulated by JNK through Ets and AP-1 Promoter Motifs*

  2006cites this paper
• Multifunctional transcription factor YY1: a therapeutic target in human cancer?

  2006cites this paper
• GA-Binding Protein and p300 Are Essential Components of a Retinoic Acid-Induced Enhanceosome in Myeloid Cells

  2006cites this paper
• Conditional Knockout Mice Reveal Distinct Functions for the Global Transcriptional Coactivators CBP and p300 in T-Cell Development

  2006cites this paper
• Identification of a novel tumor necrosis factor α‐responsive region in the NCF2 promoter

  2005cites this paper
• RUNX1/AML1 functions and mechanisms regulating granulocyte-macrophage colony-stimulating factor transcription

  2005cites this paper
• Associations and Interactions between Ets-1 and Ets-2 and Coregulatory Proteins, SRC-1, AIB1, and NCoR in Breast Cancer

  2005cites this paper
• Structure and Function of the CBP/p300 TAZ Domains

  2005cites this paper
• UMEÅ UNIVERSITY MEDICAL DISSERTATIONS

  2005cites this paper
• Spatio‐temporal expression of immediate early genes in the central nervous system of SJL/J mice

  2005cites this paper
• PTHrP gene expression in cancer: do all paths lead to Ets?

  2005cites this paper