Abstract The nucleotide sequence of the complete genome of a cyanobacterium, Microcystis aeruginosa NIES-843, was determined. The genome of M. aeruginosa is a single, circular chromosome of 5 842 795 base pairs (bp) in length, with an average GC content of 42.3%. The chromosome comprises 6312 putative protein-encoding genes, two sets of rRNA genes, 42 tRNA genes representing 41 tRNA species, and genes for tmRNA, the B subunit of RNase P, SRP RNA, and 6Sa RNA. Forty-five percent of the putative protein-encoding sequences showed sequence similarity to genes of known function, 32% were similar to hypothetical genes, and the remaining 23% had no apparent similarity to reported genes. A total of 688 kb of the genome, equivalent to 11.8% of the entire genome, were composed of both insertion sequences and miniature inverted-repeat transposable elements. This is indicative of a plasticity of the M. aeruginosa genome, through a mechanism that involves homologous recombination mediated by repetitive DNA elements. In addition to known gene clusters related to the synthesis of microcystin and cyanopeptolin, novel gene clusters that may be involved in the synthesis and modification of toxic small polypeptides were identified. Compared with other cyanobacteria, a relatively small number of genes for two component systems and a large number of genes for restriction-modification systems were notable characteristics of the M. aeruginosa genome.
Complete Genomic Structure of the Bloom-forming Toxic Cyanobacterium Microcystis aeruginosa NIES-843
T. Kaneko,N. Nakajima,Shinobu Okamoto,I. Suzuki,Yuuhiko Tanabe,M. Tamaoki,Yasukazu Nakamura,F. Kasai,A. Watanabe,K. Kawashima,Y. Kishida,Akiko Ono,Y. Shimizu,C. Takahashi,Chiharu Minami,Tsunakazu Fujishiro,Mitsuyo Kohara,M. Katoh,N. Nakazaki,S. Nakayama,M. Yamada,S. Tabata,M. Watanabe
Published 2008 in DNA Research
ABSTRACT
PUBLICATION RECORD
- Publication year
2008
- Venue
DNA Research
- Publication date
2008-01-11
- Fields of study
Biology, Medicine, Environmental Science
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- circular chromosome
A chromosome whose DNA forms a closed circular molecule rather than a linear one.
Aliases: single circular chromosome
- genome plasticity
The capacity of the genome to undergo structural change and rearrangement.
Aliases: plasticity of the genome
- microcystis aeruginosa nies-843 genome
The complete genomic DNA content of the bloom-forming cyanobacterium Microcystis aeruginosa strain NIES-843.
Aliases: M. aeruginosa NIES-843 genome
- noncoding rna genes
Genes in the genome that encode structural or catalytic RNAs rather than proteins.
Aliases: rRNA genes, tRNA genes, tmRNA, RNase P B subunit, SRP RNA, 6Sa RNA
- protein-encoding genes
Open reading frames in the genome that are annotated as putative protein-coding sequences.
Aliases: putative protein-encoding genes, protein-coding genes
- repetitive dna elements
Repeated mobile or transposable DNA sequences present in the genome.
Aliases: insertion sequences, miniature inverted-repeat transposable elements, MITEs
- restriction-modification systems
Defense systems that pair DNA restriction enzymes with modification enzymes such as methyltransferases.
Aliases: restriction-modification system genes
- toxin biosynthesis gene clusters
Clusters of neighboring genes associated with the synthesis or modification of toxic small polypeptides.
Aliases: microcystin cluster, cyanopeptolin cluster, novel gene clusters
- two-component systems
Signal transduction systems typically made of a sensor kinase and a response regulator.
Aliases: two component systems
REFERENCES
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