The FcεRI signaling cascade and integrin trafficking converge at patterned ligand surfaces

Surface-patterned ligands reveal spatially targeted signaling events mediated by FcεRI on mast cells. Distinctively clustered IgE-FcεRI recruits signaling proteins, including Syk, LAT, and activated PLCγ1. β1- and β3-integrins also colocalize at patterned sites, involving actin polymerization and stimulated trafficking from recycling endosomes.

• Publication year

  2017

• Venue

  Molecular Biology of the Cell

• Publication date

  2017-08-09

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1091/mbc.E17-03-0208PMID 28794269PMCID 5687038
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Small GTPase Rab 21 regulates cell adhesion and controls endosomal traffi c of β 1-integrins

  2019cited by this paper
• Loss of Bladder Epithelium Induced by Cytolytic Mast Cell Granules.

  2016cited by this paper
• Functional nanoscale coupling of Lyn kinase with IgE-FcεRI is restricted by the actin cytoskeleton in early antigen-stimulated signaling

  2016cited by this paper
• Different activation signals induce distinct mast cell degranulation strategies.

  2016cited by this paper
• Nanodomains in early and later phases of FcɛRI signalling.

  2015cited by this paper
• Actin foci facilitate activation of the phospholipase C-γ in primary T lymphocytes via the WASP pathway

  2015cited by this paper
• Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence

  2015influential reference
• Mast cells and dendritic cells form synapses that facilitate antigen transfer for T cell activation

  2015cited by this paper
• T cell antigen receptor activation and actin cytoskeleton remodeling.

  2014cited by this paper
• Spatially defined EGF receptor activation reveals an F-actin-dependent phospho-Erk signaling complex.

  2014influential reference
• Distinct stages of stimulated FcεRI receptor clustering and immobilization are identified through superresolution imaging.

  2013cited by this paper
• Cytoskeleton in Mast Cell Signaling

  2012cited by this paper
• The Frontiers Publishing Paradigm

  2012cited by this paper
• Quorum-Sensing in CD4+ T Cell Homeostasis: A Hypothesis and a Model

  2012cited by this paper
• Immunoglobulin Analysis Tool: A Novel Tool for the Analysis of Human and Mouse Heavy and Light Chain Transcripts

  2012cited by this paper
• Molecular mechanisms of spontaneous and directed mast cell motility

  2012cited by this paper
• Roles for Ca2+ Mobilization and Its Regulation in Mast Cell Functions

  2012cited by this paper
• Cellular responses to patterned poly(acrylic acid) brushes.

  2011cited by this paper
• Giving off mixed signals—Distinct functions of α5β1 and αvβ3 integrins in regulating cell behaviour

  2009cited by this paper
• Rab-coupling protein coordinates recycling of α5β1 integrin and EGFR1 to promote cell migration in 3D microenvironments

  2008cited by this paper
• Structural determinants for partitioning of lipids and proteins between coexisting fluid phases in giant plasma membrane vesicles.

  2008cited by this paper
• Focal adhesion proteins connect IgE receptors to the cytoskeleton as revealed by micropatterned ligand arrays

  2008influential reference
• Actin restricts FcɛRI diffusion and facilitates antigen-induced receptor immobilization

  2008cited by this paper
• Differential targeting of secretory lysosomes and recycling endosomes in mast cells revealed by patterned antigen arrays

  2007influential reference
• Focal Adhesion

  2007influential reference
• Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of β1-integrins

  2006cited by this paper
• SLP76 and SLP65: complex regulation of signalling in lymphocytes and beyond

  2006cited by this paper
• Disruption of SLP-76 Interaction with Gads Inhibits Dynamic Clustering of SLP-76 and FcεRI Signaling in Mast Cells

  2006cited by this paper
• Molecular regulation of mast cell activation.

  2006cited by this paper
• Distinct roles for the linker region tyrosines of Syk in FcepsilonRI signaling in primary mast cells.

  2005cited by this paper
• Distinct Roles for the Linker Region Tyrosines of Syk in FcϵRI Signaling in Primary Mast Cells*

  2005cited by this paper
• Temporally resolved interactions between antigen-stimulated IgE receptors and Lyn kinase on living cells

  2005cited by this paper
• Visualization of plasma membrane compartmentalization with patterned lipid bilayers.

  2004influential reference
• Dysregulated FcepsilonRI signaling and altered Fyn and SHIP activities in Lyn-deficient mast cells.

  2004cited by this paper
• Dysregulated FcεRI Signaling and Altered Fyn and SHIP Activities in Lyn-Deficient Mast Cells1

  2004cited by this paper
• Mast cells

  2004cited by this paper
• Antigen‐Stimulated Trafficking from the Recycling Compartment to the Plasma Membrane in RBL Mast Cells

  2003influential reference
• Impaired signaling via the high-affinity IgE receptor in Wiskott-Aldrich syndrome protein-deficient mast cells.

  2003cited by this paper
• Differential expression of integrin subunits on adherent and nonadherent mast cells.

  2003cited by this paper
• Mast Cell Activation on Patterned Lipid Bilayers of Subcellular Dimensions

  2003cited by this paper
• Supported lipid membranes

  2003cited by this paper
• FceRI-mediated Association of 6-1 xm Beads with RBL-2 H 3 Mast Cells Results in Exclusion of Signaling Proteins from the Forming Phagosome and Abrogation of Normal Downstream Signaling

  2002cited by this paper
• Microfilament‐dependent movement of the β3 integrin subunit within focal contacts of endothelial cells

  2002cited by this paper
• Tyrosine phosphorylation of the linker for activator of T cells in mast cells by stimulation with the high affinity IgE receptor.

  2001cited by this paper
• EGF-dependent translocation of green fluorescent protein-tagged PLC-gamma1 to the plasma membrane and endosomes.

  2001cited by this paper
• Association of Grb2, Gads, and Phospholipase C-γ1 with Phosphorylated LAT Tyrosine Residues

  2000cited by this paper
• Interactions between Fc(epsilon)RI and lipid raft components are regulated by the actin cytoskeleton.

  2000cited by this paper
• Oriented endocytic recycling of alpha5beta1 in motile neutrophils.

  2000cited by this paper
• LAT is essential for Fc(epsilon)RI-mediated mast cell activation.

  2000cited by this paper
• Association of Grb2, Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues. Effect of LAT tyrosine mutations on T cell angigen receptor-mediated signaling.

  2000cited by this paper
• Oriented endocytic recycling of α5β1 in motile neutrophils

  2000cited by this paper
• LAT Is Essential for FcεRI-Mediated Mast Cell Activation

  2000cited by this paper
• Topographical Patterning of Chemically Sensitive Biological Materials Using a Polymer-Based Dry Lift Off

  2000cited by this paper
• Molecular Mechanisms

  1999cited by this paper
• The role of actin microfilaments in the down-regulation of the degranulation response in RBL-2H3 mast cells.

  1999cited by this paper
• Functional analysis of LAT in TCR-mediated signaling pathways using a LAT-deficient Jurkat cell line.

  1999cited by this paper
• Induction and enhancement of Fc(epsilon)RI-dependent mast cell degranulation following coculture with activated T cells: dependency on ICAM-1- and leukocyte function-associated antigen (LFA)-1-mediated heterotypic aggregation.

  1998cited by this paper
• Wortmannin-sensitive phosphorylation, translocation, and activation of PLCgamma1, but not PLCgamma2, in antigen-stimulated RBL-2H3 mast cells.

  1998cited by this paper
• Wortmannin-Sensitive Phosphorylation, Translocation, and Activation of PLCγ1, but Not PLCγ2, in Antigen-stimulated RBL-2H3 Mast Cells

  1998cited by this paper
• LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway.

  1998cited by this paper
• LAT Is Required for TCR-Mediated Activation of PLCγ1 and the Ras Pathway

  1998influential reference
• Increased degranulation and phospholipase A2, C, and D activity in RBL cells stimulated through FcepsilonR1 is due to spreading and not simply adhesion.

  1997cited by this paper
• Increased degranulation and phospholipase A 2 , C , and D activity in RBL cells stimulated through Fc ε R 1 is due to spreading and not simply adhesion

  1997influential reference
• Mast cells.

  1997cited by this paper
• Human Leukaemic (HMC‐1) and Normal Skin Mast Cells Express β2‐Integrins: Characterization of β2‐Integrins and ICAM‐1 on HMC‐1 Cells

  1997cited by this paper
• VLA-beta 1 integrin subunit-specific monoclonal antibodies MB1.1 and MB1.2: binding to epitopes not dependent on thymocyte development or regulated by phorbol ester and divalent cations.

  1996cited by this paper
• Conformational changes induced in the protein tyrosine kinase p72syk by tyrosine phosphorylation or by binding of phosphorylated immunoreceptor tyrosine-based activation motif peptides

  1996cited by this paper
• VLA-β1 Integrin Subunit-Specific Monoclonal Antibodies MB1.1 and MB1.2: Binding to Epitopes Not Dependent on Thymocyte Development or Regulated by Phorbol Ester and Divalent Cations

  1996cited by this paper
• Fc epsilon RI-mediated association of 6-micron beads with RBL-2H3 mast cells results in exclusion of signaling proteins from the forming phagosome and abrogation of normal downstream signaling

  1996cited by this paper
• Transfection of Syk protein tyrosine kinase reconstitutes high affinity IgE receptor-mediated degranulation in a Syk-negative variant of rat basophilic leukemia RBL-2H3 cells

  1996cited by this paper
• Expression and function of fibronectin binding integrins on rat mast cells.

  1995influential reference
• Circulating integrins: alpha 5 beta 1, alpha 6 beta 4 and Mac‐1, but not alpha 3 beta 1, alpha 4 beta 1 or LFA‐1.

  1992cited by this paper
• Adherence of rat basophilic leukemia (RBL-2H3) cells to fibronectin-coated surfaces enhances secretion.

  1992influential reference
• Regulation of the antigen-induced F-actin response in rat basophilic leukemia cells by protein kinase C

  1991cited by this paper
• Adhesive recognition sequences.

  1991cited by this paper
• Cross-linking of receptor-bound IgE to aggregates larger than dimers leads to rapid immobilization

  1986cited by this paper
• Monoclonal dinitrophenyl-specific murine IgE antibody: preparation, isolation, and characterization.

  1980cited by this paper

• Synergy between membrane topography and domains to control signaling protein localization in mast cells facilitates their activation

  2024cites this paper
• Suppression of FcεRI-evoked Degranulation in RBL-2H3 Cells on Gelatin Methacryloyl Hydrogel

  2024cites this paper
• Antigen properties shape organization of FcεRI aggregates to tune mast cell signaling

  2023influential citation
• Lipid Driven Inter-leaflet Coupling of Plasma Membrane Order Regulates FcεRI Signaling in Mast Cells.

  2023cites this paper
• Antigen Geometry Tunes Mast Cell Signaling Through Distinct Fc ε RI Aggregation and Structural Changes

  2023cites this paper
• Microcontact Printing of Biomolecules on Various Polymeric Substrates: Limitations and Applicability for Fluorescence Microscopy and Subcellular Micropatterning Assays

  2022cites this paper
• Biofunctional nanodot arrays in living cells uncover synergistic co-condensation of Wnt signalodroplets

  2022cites this paper
• iTRVZ: liquid nano-platform for signal integration on the plasma membrane

  2021influential citation
• The role of the tyrosine kinase Lyn in allergy and cancer.

  2021cites this paper
• Innate immune receptor clustering and its role in immune regulation

  2021cites this paper
• The actin cytoskeleton and mast cell function.

  2021cites this paper
• Subcellular micropatterning for visual immunoprecipitation reveals differences in cytosolic protein complexes downstream the EGFR

  2021cites this paper
• Tuning IgE: IgE-Associating Molecules and Their Effects on IgE-Dependent Mast Cell Reactions

  2021cites this paper
• Lipid-based and protein-based interactions synergize transmembrane signaling stimulated by antigen clustering of IgE receptors

  2021cites this paper
• Signaling Enzymes and Ion Channels Being Modulated by the Actin Cytoskeleton at the Plasma Membrane

  2021cites this paper
• Subcellular Dynamic Immunopatterning of Cytosolic Protein Complexes on Microstructured Polymer Substrates

  2021cites this paper
• Micropatterned Ligand Arrays to Investigate Spatial Regulation of Cellular Signaling Initiated by Clustered Fc Receptors.

  2021cites this paper
• Basic Amino Acids Within the Juxtamembrane Domain of the Epidermal Growth Factor Receptor Regulate Receptor Dimerization and Auto-phosphorylation

  2020cites this paper
• Lipid-based, protein-based, and steric interactions synergize to facilitate transmembrane signaling stimulated by antigen-clustering of IgE receptors

  2020cites this paper
• Secretion of Mast Cell Inflammatory Mediators Is Enhanced by CADM1-Dependent Adhesion to Sensory Neurons

  2019cites this paper
• Long acyl chain ceramides govern cholesterol and cytoskeleton dependence of membrane outer leaflet dynamics

  2019cites this paper
• New roles and controls of mast cells.

  2018cites this paper
• Regulation of exocytosis and mitochondrial relocalization by Alpha-synuclein in a mammalian cell model

  2018cites this paper
• FcRγ deficiency improves survival in experimental sepsis by down-regulating TLR4 signaling pathway

  2018cites this paper
• Spatially modulated ephrinA1:EphA2 signaling increases local contractility and global focal adhesion dynamics to promote cell motility

  2018cites this paper
• Mechanisms of epidermal growth factor receptor signaling as characterized by patterned ligand activation and mutational analysis.

  2017cites this paper