Soil bacterial and fungal communities respond differently to various isothiocyanates added for biofumigation

The meals from many oilseed crops have potential for biofumigation due to their release of biocidal compounds such as isothiocyanates (ITCs). Various ITCs are known to inhibit numerous pathogens; however, much less is known about how the soil microbial community responds to the different types of ITCs released from oilseed meals (SMs). To simulate applying ITC-releasing SMs to soil, we amended soil with 1% flax SM (contains no biocidal chemicals) along with four types of ITCs (allyl, butyl, phenyl, and benzyl ITC) in order to determine their effects on soil fungal and bacterial communities in a replicated microcosm study. Microbial communities were analyzed based on the ITS region for fungi and 16S rRNA gene for bacteria using qPCR and tag-pyrosequencing with 454 GS FLX titanium technology. A dramatic decrease in fungal populations (~85% reduction) was observed after allyl ITC addition. Fungal community compositions also shifted following ITC amendments (e.g., Humicola increased in allyl and Mortierella in butyl ITC amendments). Bacterial populations were less impacted by ITCs, although there was a transient increase in the proportion of Firmicutes, related to bacteria know to be antagonistic to plant pathogens, following amendment with allyl ITC. Our results indicate that the type of ITC released from SMs can result in differential impacts on soil microorganisms. This information will aid selection and breeding of plants for biofumigation-based control of soil-borne pathogens while minimizing the impacts on non-target microorganisms.

• Publication year

  2015

• Venue

  Frontiers in Microbiology

• Publication date

  2015-01-07

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3389/fmicb.2014.00729PMID 25709600PMCID 4288022
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Differential impacts of brassicaceous and nonbrassicaceous oilseed meals on soil bacterial and fungal communities.

  2013influential reference
• Impact of Indian Mustard (Brassica juncea) and Flax (Linum usitatissimum) Seed Meal Applications on Soil Carbon, Nitrogen, and Microbial Dynamics

  2012cited by this paper
• Control of cotton Verticillium wilt and fungal diversity of rhizosphere soils by bio-organic fertilizer

  2012cited by this paper
• Microbial communities in rice rhizosphere altered by intermittent and continuous flooding in fields with long-term arsenic application

  2011influential reference
• Inhibition of the germination and growth of Phymatotrichopsis omnivora (cotton root rot) by oilseed meals and isothiocyanates

  2011cited by this paper
• Active and total microbial communities in forest soil are largely different and highly stratified during decomposition

  2011cited by this paper
• Reducing the Effects of PCR Amplification and Sequencing Artifacts on 16S rRNA-Based Studies

  2011cited by this paper
• The Impact of Biofumigation and Chemical Fumigation Methods on the Structure and Function of the Soil Microbial Community

  2010cited by this paper
• QIIME allows analysis of high-throughput community sequencing data

  2010cited by this paper
• Indoor fungal composition is geographically patterned and more diverse in temperate zones than in the tropics

  2010cited by this paper
• Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities

  2009cited by this paper
• Pesticide tolerance of Paenibacillus sp. D1 and its chitinase.

  2009cited by this paper
• Postharvest treatment of isothiocyanates to control Alternaria rot in netted melon

  2009influential reference
• Dictionary of the Fungi

  2008cited by this paper
• Glucosinolates and biofumigation: fate of glucosinolates and their hydrolysis products in soil

  2008cited by this paper
• Bacterial and fungal contributions to soil nitrogen cycling under Douglas fir and red alder at two sites in Oregon

  2008influential reference
• Tag-encoded pyrosequencing analysis of bacterial diversity in a single soil type as affected by management and land use

  2008cited by this paper
• Saprophytic and pathogenic behaviour of R. solani AG2-1 (ZG-5) in a soil amended with Diplotaxis tenuifolia or Brassica nigra manures and incubated at different temperatures and soil water content

  2007cited by this paper
• Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences

  2006cited by this paper
• Ionic thiocyanate (SCN-) production from 4-hydroxybenzyl glucosinolate contained in Sinapis alba seed meal.

  2005influential reference
• Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays

  2005cited by this paper
• Biofumigation: environmental impacts on the biological activity of diverse pure and plant-derived isothiocyanates.

  2005influential reference
• Assessment of the diversity of Paenibacillus species in environmental samples by a novel rpoB-based PCR-DGGE method.

  2005cited by this paper
• 2-Phenylethylisothiocyanate concentration and bacterial community composition in the rhizosphere of field-grown canola.

  2004cited by this paper
• Cross-enhancement: enhanced biodegradation of isothiocyanates in soils previously treated with metham sodium

  2003cited by this paper
• 2-Phenylethylisothiocyanate concentration and microbial community composition in the rhizosphere of canola

  2003cited by this paper
• Fungal ingrowth on forest floor and decomposing needle litter of Chamaecyparis obtusa in relation to resource availability and moisture condition

  2003influential reference
• In vitro inhibition of soil microorganisms by 2-phenylethyl isothiocyanate

  2002cited by this paper
• Impact of Fumigants on Soil Microbial Communities

  2001influential reference
• PAST: paleontological statistics software package for education and data analysis version 2.09

  2001influential reference
• PAST: PALEONTOLOGICAL STATISTICAL SOFTWARE PACKAGE FOR EDUCATION AND DATA ANALYSIS

  2001influential reference
• Suppressive activity of some glucosinolate enzyme degradation products on Pythium irregulare and Rhizoctonia solani in sterile soil

  2000cited by this paper
• Hydrolytic enzyme activity of Paenibacillus sp. strain B2 and effects of the antagonistic bacterium on cell integrity of two soil-borne pathogenic fungi

  2000cited by this paper
• Diversity of Paenibacillus polymyxa populations in the rhizosphereof wheat (Triticum durum) in Algerian soils

  2000cited by this paper
• Inhibition of soil nitrifying bacteria communities and their activities by glucosinolate hydrolysis products

  2000influential reference
• Design of a primer for ribosomal DNA internal transcribed spacer with enhanced specificity for ascomycetes.

  1999cited by this paper
• Inhibition of Pythium ultimum and Rhizoctonia solani by Shredded Leaves of Brassica Species

  1999cited by this paper
• Soil suppressiveness to Fusarium wilt: influence of a cover-plant on density and diversity of Fusarium populations.

  1998cited by this paper
• Toxicity of Isothiocyanates Produced by Glucosinolates in Brassicaceae Species to Black Vine Weevil Eggs

  1998cited by this paper
• Inhibition of Aphanomyces euteiches f. sp. pisi by Volatiles Produced by Hydrolysis of Brassica napus Seed Meal.

  1997cited by this paper
• Suppression of soil-borne cereal pathogens and inhibition of wheat germination by mustard seed meal.

  1996influential reference
• Biofumigation: Isothiocyanates released frombrassica roots inhibit growth of the take-all fungus

  1994cited by this paper
• The effects of fungicides on soil fungal populations

  1979cited by this paper
• Studies on soil fumigation—IV: Effects on fungi

  1976cited by this paper
• Isothiocyanates, nitriles and thiocyanates as products of autolysis of glucosinolates in Cruciferae

  1976influential reference

• Combined Effects of Soil Disinfestants and Foliar Biostimulants on Growth and Quality of Baby Leaf Lettuce (Lactuca sativa L.)

  2026cites this paper
• Efficacy of Liquid Brassica Seed Meal Formulations Against Phytophthora cinnamomi in Laboratory and Field Conditions in an Ink‐Diseased Cork Oak Forest

  2025cites this paper
• Agronomic evaluation of hydrogels containing antimicrobial agents applied on Lactuca sativa L. seeds and plantlets

  2025cites this paper
• High-throughput sequencing metabarcoding and network analysis elucidate the effects of soil fumigation and biostimulant on potato yield, rhizoctonia canker, and fungal community

  2025cites this paper
• Pseudomonas virulence factor SaxA detoxifies plant glucosinolate hydrolysis products, rescuing a commensal that suppresses virulence gene expression

  2025cites this paper
• Glucosinolate structural diversity shapes recruitment of a metabolic network of leaf-associated bacteria

  2024cites this paper
• Effect of rhizosphere soil microbial communities and environmental factors on growth and the active ingredients of Angelica sinensis in Gansu Province, China

  2024cites this paper
• Mechanism of KOH-Modified Biochar Application Under Water-Saving and Nitrogen-Reducing Conditions to Inhibit Soybean Root Rot and Improve Quality And Efficiency

  2024cites this paper
• Abutilon theophrasti’s Resilience against Allelochemical-Based Weed Management in Sustainable Agriculture – Due to Collection of Highly Advantageous Microorganisms?

  2023cites this paper
• In vitro application of Eruca vesicaria subsp. sativa leaf extracts and associated metabolites reduces the growth of Oomycota species involved in Kiwifruit Vine Decline Syndrome

  2023cites this paper
• Using Brassica Cover Crops as Living Mulch in a Vineyard, Changes over One Growing Season

  2023cites this paper
• The pesticidal activity of Spider plant, Cleome gynandra L, plant tissue on soil borne pathogens

  2023cites this paper
• Application of plant specialized metabolites to modulate soil microbiota

  2023cites this paper
• Bioactivity of brassica seed meals and its compounds as ecofriendly larvicides against mosquitoes

  2023cites this paper
• Green Manures Alter Taxonomic and Functional Characteristics of Soil Bacterial Communities

  2022cites this paper
• Soil microbiota promotes early developmental stages of Phelipanche ramosa L. Pomel during plant parasitism on Brassica napus L.

  2022cites this paper
• Legacy effects of fumigation on soil bacterial and fungal communities and their response to metam sodium application

  2022cites this paper
• The Effect of Biofumigation with Various Brassicaceae Plants on the Number and Diversity of Soil-Borne Fungi in Arish City, Egypt

  2022cites this paper
• Mechanism of the antifungal action of chloropicrin fumigation against Panax notoginseng root rot caused by Fusarium solani

  2022cites this paper
• Chemical fumigation and biofumigation alter soil bacterial community diversity and composition.

  2022cites this paper
• Glucosinolates as an effective tool in plant-parasitic nematodes control: Exploiting natural plant defenses

  2022cites this paper
• Changes in the root fungal microbiome of strawberry following application of residues of the biofumigant oilseed radish

  2021cites this paper
• Influence of chemical fumigation and biofumigation on soil nitrogen cycling processes and nitrifier and denitrifier abundance

  2021cites this paper
• Integrated analysis reveals an association between the rhizosphere microbiome and root rot of arecanut palm

  2021cites this paper
• Interactions between Brassica Biofumigants and Soil Microbiota: Causes and Impacts.

  2021cites this paper
• Effects of Glucosinolate-Derived Isothiocyanates on Fungi: A Comprehensive Review on Direct Effects, Mechanisms, Structure-Activity Relationship Data and Possible Agricultural Applications

  2021cites this paper
• Plant pathogenic bacterium can rapidly evolve tolerance to an antimicrobial plant allelochemical

  2021cites this paper
• Comprehensive analysis of the apple rhizobiome as influenced by different Brassica seed meals and rootstocks in the same soil/plant system

  2021cites this paper
• Prokaryotic Communities in Saline Soils of the Lake Elton Area in a Soil Catena along the Khara River

  2020cites this paper
• The Trichoderma viride F-00612 consortium tolerates 2-amino-3H-phenoxazin-3-one and degrades nitrated benzo[d]oxazol-2(3H)-one

  2020cites this paper
• Biofumigation for Fighting Replant Disease- A Review

  2020cites this paper
• Biofumigation: An alternative strategy for the control of plant parasitic nematodes

  2020cites this paper
• Plant-derived allyl isothiocyanate enrich biocontrol microbes of tomato plant rhizosphere in vegetable greenhouse

  2020influential citation
• Prickly Ash Seed Kernel: A New Bio-Fumigation Material Against Tobacco Black Shank

  2020influential citation
• Biofumigation—an environment-friendly soil fumigation technology

  2020cites this paper
• Observations on early fungal infections with relevance for replant disease in fine roots of the rose rootstock Rosa corymbifera 'Laxa'

  2020cites this paper
• Arabidopsis assemble distinct root-associated microbiomes through the synthesis of an array of defense metabolites

  2020cites this paper
• Soil Microbial Biomass and Fungi Reduced With Canola Introduced Into Long-Term Monoculture Wheat Rotations

  2019cites this paper
• Fluazinam positively affected the microbial communities in clubroot cabbage rhizosphere

  2019cites this paper
• Evaluating biofumigant crops as a pre-plant treatment for avocado orchard establishment in sites with high Phytophthora root rot pressure

  2019cites this paper
• Fungal Community Structural and Microbial Functional Pattern Changes After Soil Amendments by Oilseed Meals of Jatropha curcas and Camelina sativa: A Microcosm Study

  2019cites this paper
• Exploring evolutionary theories of plant defence investment using field populations of the deadly carrot.

  2019cites this paper
• Soil Bacterial Community Was Changed after Brassicaceous Seed Meal Application for Suppression of Fusarium Wilt on Pepper

  2018cites this paper
• Evaluation of allyl isothiocyanate as a soil fumigant against soil-borne diseases in commercial tomato (Lycopersicon esculentum Mill.) production in China.

  2018cites this paper
• Growth of the fungus Chaetomium aureum in the presence of lead: implications in bioremediation

  2018cites this paper
• Conversion from natural wetlands to forestland and farmland alters the composition of soil fungal communities in Sanjiang Plain, Northeast China

  2018cites this paper
• Disruption of microbial community composition and identification of plant growth promoting microorganisms after exposure of soil to rapeseed-derived glucosinolates

  2018cites this paper
• Soil Salinity Controls Relative Abundance of Specific Bacterial Groups Involved in the Decomposition of Maize Plant Residues

  2018cites this paper
• Effects of Soil Pre-Treatment with Basamid® Granules, Brassica juncea, Raphanus sativus, and Tagetes patula on Bacterial and Fungal Communities at Two Apple Replant Disease Sites

  2017influential citation
• Effects of soil pre-treatment with Basamid© granules, Brassica juncea, Raphanus sativus, and Tagetes patula on bacterial and fungal communities at two apple replant

  2017influential citation
• Fall Rye Reduced Residual Soil Nitrate and Dryland Spring Wheat Grain Yield

  2017cites this paper
• Soil microbial communities in cucumber monoculture and rotation systems and their feedback effects on cucumber seedling growth

  2017cites this paper
• Effects of nitrification inhibitor 3,4-dimethylpyrazole phosphate and fungicide iprodione on soil fungal biomass and community: based on internal transcribed spacer region

  2017cites this paper
• Lipopeptides produced by B. amyloliquefaciens NJN-6 altered the soil fungal community and non-ribosomal peptides genes harboring microbial community

  2017cites this paper
• Effects of biofumigation using Brassica juncea and Raphanus sativus in comparison to disinfection using Basamid on apple plant growth and soil microbial communities at three field sites with replant disease

  2016cites this paper
• Yucatán in black and red: Linking edaphic analysis and pyrosequencing-based assessment of bacterial and fungal community structures in the two main kinds of soil of Yucatán State.

  2016cites this paper
• Amplicon-based metabarcoding reveals temporal response of soil microbial community to fumigation-derived products

  2016influential citation
• Microbial biomass, fungal and bacterial residues, and their relationships to the soil organic matter C/N/P/S ratios

  2016cites this paper
• Effects of the biocides on the culturable house dust-borne bacterial compositions and diversities

  2016cites this paper
• Effect of Brassica Biofumigant Amendments on Different Stages of the Life Cycle of Phytophthora cinnamomi

  2016cites this paper
• Degradation of Biofumigant Isothiocyanates and Allyl Glucosinolate in Soil and Their Effects on the Microbial Community Composition

  2015cites this paper
• Studies on plant-microbe interaction to improve stress tolerance in plants for sustainable agriculture

  2015cites this paper