Identification of a New Acute Phase Protein (*)

We have previously reported mouse SIP24 protein as a secreted inducible protein produced by quiescent Balb/c 3T3 cells. SIP24 can be produced in response to many factors, including serum, basic fibroblast growth factor, prostaglandin F2α, phorbol ester, and dexamethasone. Here we present evidence to show that SIP24 is the product of mouse 24P3 mRNA. The 24P3 cDNA was originally cloned from an SV40-transformed quiescent mouse primary kidney cell culture, and it has been classified as a new member of the lipocalin protein family. We show that the SIP24/24P3 protein and mRNA increase dramatically in mouse serum and liver during the acute phase response induced by turpentine injection. Injection of mice with dexamethasone caused a modest increase of SIP24/24P3 mRNA in the liver. Tissue distribution studies revealed that SIP24/24P3 is mainly expressed in liver during the acute phase response. SIP24/24P3 was also detected in the brain and the uterus. In mouse BNL (Balb/c normal liver) cells, the production of SIP24/24P3 is stimulated by tumor necrosis factor α, which is a major regulator of the expression of other acute phase proteins. From its pattern of regulation, we conclude that SIP24/24P3 is a new type 1 acute phase protein.

• Publication year

  1995

• Venue

  Journal of Biological Chemistry

• Publication date

  1995-09-22

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.270.38.22565PMID 7545679
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Regulation of the Acute Phase Response by Cytokines

  2015cited by this paper
• Characterization of cloned rat ribosomal DNA fragments

  2004cited by this paper
• The acute phase response.

  1994cited by this paper
• The acute phase response.

  1994cited by this paper
• Functional NF-kappa B element in rabbit serum amyloid A gene and its role in acute phase induction.

  1993cited by this paper
• Functional NF-κB Element in Rabbit Serum Amyloid A Gene and Its Role in Acute-Phase Induction

  1993cited by this paper
• Identification by microsequencing of lipopolysaccharide-induced proteins secreted by mouse macrophages.

  1993cited by this paper
• Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase.

  1993cited by this paper
• IL6 and the acute phase reaction.

  1992cited by this paper
• IL‐6 and NF‐IL6 in Acute‐Phase Response and Viral Infection

  1992cited by this paper
• Extrahepatic synthesis of acute phase proteins and their functions.

  1992cited by this paper
• Mouse oncogene protein 24p3 is a member of the lipocalin protein family.

  1991cited by this paper
• Basic fibroblast growth factor induces 3T3 fibroblasts to synthesize and secrete a cyclophilin-like protein and β2-microglobulin

  1991cited by this paper
• Basic fibroblast growth factor induces 3T3 fibroblasts to synthesize and secrete a cyclophilin-like protein and beta 2-microglobulin.

  1991cited by this paper
• Antiinflammatory effects of polypeptide growth factors. Platelet-derived growth factor, epidermal growth factor, and fibroblast growth factor inhibit the cytokine-induced expression of the alternative complement pathway activator factor B in human fibroblasts.

  1990cited by this paper
• Molecular characterization and acute phase expression of the multiple Mus caroli alpha 1-acid glycoprotein (AGP) genes. Differences in glucocorticoid stimulation and regulatory elements between the rat and mouse AGP genes.

  1990cited by this paper
• SV40-induced expression of mouse gene 24p3 involves a post-transcriptional mechanism.

  1989cited by this paper
• Phorbol ester modulates interleukin 6- and interleukin 1-regulated expression of acute phase plasma proteins in hepatoma cells.

  1988cited by this paper
• [39] Detection of proteins induced by growth regulators

  1987cited by this paper
• Guanidine Isothiocyanate Preparation of Total RNA

  1986cited by this paper
• Basic methods in molecular biology

  1986cited by this paper
• Superinduction by cycloheximide of mitogen‐induced secreted proteins produced by Balb/c 3T3 cells

  1985cited by this paper
• Induction of rat α2‐macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell‐derived factor

  1984cited by this paper
• Plasma protein secretion by the liver

  1983cited by this paper
• The structure and expression of very closely related members of the alpha 2u globulin gene family.

  1982cited by this paper
• Rapid selective stimulation by growth factors of the incorporation by BALB/C 3T3 cells of [35S]methionine into a glycoprotein and five superinducible proteins.

  1982cited by this paper
• "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

  1981cited by this paper
• Prostaglandins in human reproduction.

  1981cited by this paper
• Specific glucocorticoid inhibition of growth promoting effects of prostaglandin F2α on 3T3 cells

  1977cited by this paper

• Antimicrobial proteins regulating neuroinflammation

  2026cites this paper
• Endothelial function/damage markers and NGAL – intraoperative assessment in blood specimens obtained from different sampling sites during open repair of abdominal aortic aneurysm

  2025cites this paper
• Lipocalin 2 deficiency impacts angiogenesis after ischemia in vivo and in vitro

  2025cites this paper
• Overview of the expression patterns and roles of Lipocalin 2 in the reproductive system

  2024cites this paper
• Editorial: Endocrine-related cardiovascular diseases: Recent advances in diagnosis and treatment

  2023cites this paper
• Lipocalin 2 receptors: facts, fictions, and myths

  2023cites this paper
• Crosstalk of Highly Purified Microglia and Astrocytes in the Frame of Toll-like Receptor (TLR)2/1 Activation

  2023cites this paper
• Host and Pathogen-Directed Therapies against Microbial Infections Using Exosome- and Antimicrobial Peptide-derived Stem Cells with a Special look at Pulmonary Infections and Sepsis

  2023cites this paper
• Neutrophil Gelatinase-Associated Lipocalin as Potential Predictive Biomarker of Melanoma and Non-Melanoma Skin Cancers in Psoriatic Patients: A Pilot Study

  2022cites this paper
• The Association between Serum Neutrophil Gelatinase-Associated Lipocalin and Depression in Patients with Type 2 Diabetes Mellitus

  2022cites this paper
• Apios americana Medikus: A novel and promising food for postpartum uterine involution

  2022cites this paper
• TOB is an effector of the hippocampus-mediated acute stress response

  2022cites this paper
• Urinary neutrophil gelatinase-associated lipocalin (NGAL) can potentially predict vascular complications and reliably risk stratify patients with peripheral arterial disease

  2022cites this paper
• Lipocalin-2 does not influence EAE clinical score but it increases inflammation in central nervous system.

  2022cites this paper
• TOB is an effector of the hippocampus-mediated acute stress response

  2022cites this paper
• Serum Lipocalin-2 Levels Are Increased and Independently Associated With Early-Stage Renal Damage and Carotid Atherosclerotic Plaque in Patients With T2DM

  2022cites this paper
• Elevated levels of neutrophil gelatinase-associated lipocalin among OCD patients: an exploratory study

  2021cites this paper
• Lipocalin-2 in Diabetic Complications of the Nervous System: Physiology, Pathology, and Beyond

  2021cites this paper
• Tonicity-responsive enhancer-binding protein promotes diabetic neuroinflammation and cognitive impairment via upregulation of lipocalin-2

  2021influential citation
• Lipocalin-2: a role in hepatic gluconeogenesis via AMP-activated protein kinase (AMPK)

  2021cites this paper
• Plasmatic lipocalin-2 levels in chronic low-grade inflammation syndromes: Comparison between metabolic syndrome, total and partial adult growth hormone deficiency.

  2020cites this paper
• Glomerular Collagen Deposition and Lipocalin-2 2 Expression Are Early Signs of Renal Injury in 3 Prediabetic Obese Rats 4

  2020cites this paper
• Cancer cells deploy lipocalin-2 to collect limiting iron in leptomeningeal metastasis

  2020cites this paper
• Chronic Carbon Tetrachloride Applications Induced Hepatocyte Apoptosis in Lipocalin 2 Null Mice through Endoplasmic Reticulum Stress and Unfolded Protein Response

  2020cites this paper
• The Lipocalin2 Gene is Regulated in Mammary Epithelial Cells by NFκB and C/EBP In Response to Mycoplasma

  2020cites this paper
• Lipocalin-2 - the myth of its expression and function.

  2020cites this paper
• Specific Commensal Bacterium Critically Regulates Gut Microbiota Osteoimmunomodulatory Actions During Normal Postpubertal Skeletal Growth and Maturation

  2020cites this paper
• Increased expression of Toll-like receptors 2, 3, 4 and 7 mRNA in the kidney and intestine of a septic mouse model

  2019cites this paper
• Assessment of serum and urinary levels of neutrophil gelatinase-associated lipocalin in correlation with albuminuria in nondiabetic obese patients

  2019cites this paper
• Liver parenchymal cells lacking Lipocalin 2 (LCN2) are prone to endoplasmic reticulum stress and unfolded protein response.

  2019cites this paper
• Early Hepatic Oxidative Stress and Mitochondrial Changes Following Western Diet in Middle Aged Rats

  2019cites this paper
• Glomerular Collagen Deposition and Lipocalin-2 Expression Are Early Signs of Renal Injury in Prediabetic Obese Rats

  2019cites this paper
• Adipose Tissue-Derived Signatures for Obesity and Type 2 Diabetes: Adipokines, Batokines and MicroRNAs

  2019cites this paper
• Lipocalin 24p3 Induction in Colitis Adversely Affects Inflammation and Contributes to Mortality

  2019cites this paper
• Elevated Lipocalin-2 Can Indicate the Vascular Inflammation in Patients with Ischemic Stroke

  2019cites this paper
• Lipocalin 2 Protects Against Escherichia coli Infection by Modulating Neutrophil and Macrophage Function

  2019cites this paper
• Evaluation of serum chitinase-3-like protein 1, pentraxin-3, and neutrophil gelatinase-associated lipocalin levels in diabetic foot cases

  2019cites this paper
• Circulating levels of lipocalin-2 are associated with fatty pancreas but not fatty liver.

  2019cites this paper
• Does Whole-Blood Neutrophil Gelatinase-Associated Lipocalin Stratify Acute Kidney Injury in Critically Ill Patients?

  2019cites this paper
• Adipose Tissue

  2019cites this paper
• N-Glycosylation of Lipocalin 2 Is Not Required for Secretion or Exosome Targeting

  2018cites this paper
• Lipocalin-2 Functions as Inhibitor of Innate Resistance to Mycobacterium tuberculosis

  2018cites this paper
• Anthropometric and glucometabolic changes in an aged mouse model of lipocalin-2 overexpression

  2018cites this paper
• Circulating Lipocalin-2 Levels Are Associated With Soluble Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Levels In Type 2 Diabetic Patients

  2018cites this paper
• Lipocalin 2: An Emerging Player in Iron Homeostasis and Inflammation.

  2017cites this paper
• Early markers of renal damage in obstructive sleep apnea syndrome (OSAS) patients with or without diabetes mellitus

  2017cites this paper
• Lipocalin-2: A Master Mediator of Intestinal and Metabolic Inflammation.

  2017cites this paper
• Cystic fluid neutrophil gelatinase-associated lipocalin (NGAL) concentration in differential diagnosis of pancreatic cystic lesions: a new factor enters the scene?

  2017cites this paper
• LCN2 overexpression in bone enhances the hematopoietic compartment via modulation of the bone marrow microenvironment

  2017cites this paper
• Serial measurements of neutrophil gelatinase-associated lipocalin: prognostic value in patients with ST-segment elevation myocardial infarction treated with a primary percutaneous coronary intervention

  2017cites this paper
• Lipocalin-2 from both myeloid cells and the epithelium combats Klebsiella pneumoniae lung infection in mice.

  2017cites this paper
• NGAL and MMP-9/NGAL as biomarkers of plaque vulnerability and targets of statins in patients with carotid atherosclerosis

  2017cites this paper
• Neutrophil gelatinase associated lipocalin (NGAL) is elevated in type 2 diabetics with carotid artery stenosis and reduced under metformin treatment

  2017cites this paper
• Lipocalin‐2 exacerbates psoriasiform skin inflammation by augmenting T‐helper 17 response

  2016cites this paper
• Serum Neutrophil Gelatinase Associated Lipocalin (NGAL) Outperforms Serum Creatinine in Detecting Sepsis-Induced Acute Kidney Injury, Experiments on Bilateral Nephrectomy and Bilateral Ureter Obstruction Mouse Models

  2016cites this paper
• Microbial invasion and histological chorioamnionitis upregulate neutrophil-gelatinase associated lipocalin in preterm prelabor rupture of membranes

  2016cites this paper
• Hormonal adaptation to acute and chronic hypoxia : the role of brain natriuretic peptide and stress hormones in the diagnosis and etiology of altitude illness

  2016cites this paper
• Neutrophil Gelatinase-Associated Lipocalin (NGAL) is Associated with Symptomatic Carotid Atherosclerosis and Drives Pro-inflammatory State In Vitro.

  2016cites this paper
• Berry intake changes hepatic gene expression and DNA methylation patterns associated with high-fat diet.

  2016cites this paper
• Levels of lipocalin-2 in crevicular fluid and tear fluid in chronic periodontitis and obesity subjects.

  2016cites this paper
• Increased Lipocalin-2 Contributes to the Pathogenesis of Psoriasis by Modulating Neutrophil Chemotaxis and Cytokine Secretion.

  2016cites this paper
• Urine/Plasma Neutrophil Gelatinase Associated Lipocalin Ratio Is a Sensitive and Specific Marker of Subclinical Acute Kidney Injury in Mice

  2016cites this paper
• Sustained expression of lipocalin-2 during polymicrobial sepsis

  2015cites this paper
• The Dietary Combination of Quercetin and Resveratrol Supplementation May Improve Exercise Tolerance in Young Untrained Males by Modulating IL-6 and NGAL Response

  2015cites this paper
• STUDYING THE ROLE OF LIPOCALIN-2 IN THE PATHOPHYSIOLOGY OF MULTIPLE SCLEROSIS – LOOKING BEYOND THE BRAIN O PAPEL DA LIPOCALIN-2 NA PATOFISIOLOGIA DA ESCLEROSE MÚLTIPLA – UM OLHAR PARA ALÉM DO CÉREBRO

  2015cites this paper
• Cytoprotective Effect of Ferritin H in Renal Ischemia Reperfusion Injury

  2015cites this paper
• Plasma Neutrophil Gelatinase-Associated Lipocalin Is Primarily Related to Inflammation during Sepsis: A Translational Approach

  2015cites this paper
• expression profile analysis The graft response to transplantation: a gene

  2015cites this paper
• The role of lipocalin‐2 in liver regeneration

  2015cites this paper
• Can serum NGAL levels be used as an inflammation marker on hemodialysis patients with permanent catheter?

  2015cites this paper
• Verhaltensbiologische Untersuchungen über die Langzeitauswirkungen von experimentellem Schädel-Hirn-Trauma in der Maus

  2015cites this paper
• Lipocalin-2 is an inflammatory biomarker associated with metabolic abnormalities in Egyptian obese children

  2015cites this paper
• Diverse functional roles of lipocalin-2 in the central nervous system.

  2015cites this paper
• Expression and localisation of brain-type organic cation transporter (BOCT/24p3R/LCN2R) in the normal rat hippocampus and after kainate-induced excitotoxicity.

  2015cites this paper
• Lipocalin 2 in the pathogenesis of fatty liver disease and nonalcoholic steatohepatitis

  2015cites this paper
• Glucocorticoid augments lipopolysaccharide-induced activation of the IκBζ-dependent genes encoding the anti-microbial glycoproteins lipocalin 2 and pentraxin 3.

  2015cites this paper
• lipopolysaccharide-treated rats during acute systemic inflammation in Differential gene expression profiling in whole blood

  2015cites this paper
• From the periphery to the brain: Lipocalin-2, a friend or foe?

  2015influential citation
• Clinical Study Neutrophil Gelatinase-Associated Lipocalin : Its Response to Hypoxia and Association with Acute Mountain Sickness

  2015cites this paper
• HGF mediated upregulation of lipocalin 2 regulates MMP9 through nuclear factor-κB activation.

  2015cites this paper
• Lipocalin-2 in the Inflammatory Activation of Brain Astrocytes.

  2015cites this paper
• Lipocalin 2, an essential multi-regulator of hepatic homeostasis during a Methionine Choline deficient diet-induced non-alcoholic steatohepatitis in mice

  2015cites this paper
• Co‐culture of bone marrow‐derived mesenchymal stem cells overexpressing lipocalin 2 with HK‐2 and HEK293 cells protects the kidney cells against cisplatin‐induced injury

  2015cites this paper
• Lipocalin-2: a New Regulator of Non-Pathogen- Associated Neuroinflammation

  2014cites this paper
• Lipocalin 2 decreases senescence of bone marrow-derived mesenchymal stem cells under sub-lethal doses of oxidative stress

  2014cites this paper
• Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2

  2014cites this paper
• Dendritic Cell-Secreted Lipocalin2 Induces CD8+ T-Cell Apoptosis, Contributes to T-Cell Priming and Leads to a TH1 Phenotype

  2014influential citation
• Lipocalin-2 Protein Deficiency Ameliorates Experimental Autoimmune Encephalomyelitis

  2014cites this paper
• Temporal Course of Changes in Gene Expression Suggests a Cytokine-Related Mechanism for Long-Term Hippocampal Alteration after Controlled Cortical Impact

  2014cites this paper
• Neutrophil gelatinase‐associated lipocalin in blood in children with inflammatory bowel disease

  2014cites this paper
• The pivotal role played by lipocalin-2 in chronic inflammatory pain.

  2014cites this paper
• Diet high in fructose leads to an overexpression of lipocalin-2 in rat fatty liver.

  2014cites this paper
• The Role of Neutrophil Gelatinase-Associated Lipocalin (Ngal) in the Determination of Contrast-Induced Nephropathy in Patients Undergoing Coronary Angiography

  2014cites this paper
• Serum neutrophil gelatinase-associated lipocalin levels are correlated with the complexity and the severity of atherosclerosis in acute coronary syndrome

  2014cites this paper
• Plasma Lipocalin Concentrations in Relation to Visceral Fat, Risk Factor for Endometrial Cancer

  2014cites this paper
• Neutrophil Gelatinase-Associated Lipocalin and depression in patients with chronic heart failure

  2014cites this paper
• Lipocalin 2 modulates the cellular response to amyloid beta

  2014cites this paper
• [Analysis of neutrophil gelatinase-associated lipocalin in the critical patient].

  2014cites this paper
• Association between Plasma Neutrophil Gelatinase Associated Lipocalin Level and Obstructive Sleep Apnea or Nocturnal Intermittent Hypoxia

  2013cites this paper
• Investigating the role of lipocalin-2 as a diagnostic indicator for nonalcoholic steatohepatitis in a fructose-induced rat fatty liver model: First experimental studies

  2013cites this paper