Structural insight into the essential PB1–PB2 subunit contact of the influenza virus RNA polymerase

No abstract is available for this paper.

• Publication year

  2009

• Venue

  EMBO Journal

• Publication date

  2009-06-17

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/emboj.2009.138PMID 19461581PMCID PMC2699363
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• RACK1, A multifaceted scaffolding protein: Structure and function

  2011cited by this paper
• Crystal structure of an avian influenza polymerase PAN reveals an endonuclease active site

  2009cited by this paper
• The cap-snatching endonuclease of influenza virus polymerase resides in the PA subunit

  2009cited by this paper
• Crystal structure of the polymerase PAC–PB1N complex from an avian influenza H5N1 virus

  2008cited by this paper
• Host factors for replication and transcription of the influenza virus genome

  2008cited by this paper
• The structural basis for an essential subunit interaction in influenza virus RNA polymerase

  2008cited by this paper
• Host Determinant Residue Lysine 627 Lies on the Surface of a Discrete, Folded Domain of Influenza Virus Polymerase PB2 Subunit

  2008cited by this paper
• The structural basis for cap binding by influenza virus polymerase subunit PB2

  2008cited by this paper
• Avian Influenza Virus (H5N1): a Threat to Human Health

  2007cited by this paper
• Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit

  2007cited by this paper
• Inference of macromolecular assemblies from crystalline state.

  2007cited by this paper
• Evidence that the C‐terminal PB2‐binding region of the influenza A virus PB1 protein is a discrete α‐helical domain

  2007cited by this paper
• Amino Acid Residues in the N-Terminal Region of the PA Subunit of Influenza A Virus RNA Polymerase Play a Critical Role in Protein Stability, Endonuclease Activity, Cap Binding, and Virion RNA Promoter Binding

  2006cited by this paper
• Characterization of the 1918 influenza virus polymerase genes

  2005cited by this paper
• Functional domains of the influenza A virus PB2 protein: identification of NP- and PB1-binding sites.

  2004cited by this paper
• Model Suggesting that Replication of Influenza Virus Is Regulated by Stabilization of Replicative Intermediates

  2004cited by this paper
• Coot: model-building tools for molecular graphics.

  2004cited by this paper
• SOLVE and RESOLVE: automated structure solution and density modification.

  2003cited by this paper
• Influenza fact sheet.

  2003cited by this paper
• Structural Basis for the Transition from Initiation to Elongation Transcription in T7 RNA Polymerase

  2002cited by this paper
• The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication.

  2002cited by this paper
• A Single Amino Acid Mutation in the PA Subunit of the Influenza Virus RNA Polymerase Inhibits Endonucleolytic Cleavage of Capped RNAs

  2002cited by this paper
• Fine mapping of the subunit binding sites of influenza virus RNA polymerase.

  2001cited by this paper
• Generation of influenza A viruses entirely from cloned cDNAs.

  1999cited by this paper
• Automated MAD and MIR structure solution

  1999cited by this paper
• Direct Evidence that the Poly(A) Tail of Influenza A Virus mRNA Is Synthesized by Reiterative Copying of a U Track in the Virion RNA Template

  1999cited by this paper
• Influenza A Virus RNA Polymerase Has the Ability To Stutter at the Polyadenylation Site of a Viral RNA Template during RNA Replication

  1999cited by this paper
• RNA‐dependent activation of primer RNA production by influenza virus polymerase: different regions of the same protein subunit constitute the two required RNA‐binding sites

  1998cited by this paper
• [20] Processing of X-ray diffraction data collected in oscillation mode.

  1997cited by this paper
• Processing of X-ray diffraction data collected in oscillation mode.

  1997cited by this paper
• Refinement of macromolecular structures by the maximum-likelihood method.

  1997cited by this paper
• Molecular assembly of the influenza virus RNA polymerase: determination of the subunit-subunit contact sites.

  1996cited by this paper
• Influenza virus polymerase basic protein 1 interacts with influenza virus polymerase basic protein 2 at multiple sites

  1996cited by this paper
• Mutational analysis identifies functional domains in the influenza A virus PB2 polymerase subunit

  1996cited by this paper
• Identification of two separate domains in the influenza virus PB1 protein involved in the interaction with the PB2 and PA subunits: a model for the viral RNA polymerase structure.

  1996cited by this paper
• Epitope mapping of cross-reactive monoclonal antibodies specific for the influenza A virus PA and PB2 polypeptides.

  1995cited by this paper
• LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions.

  1995cited by this paper
• Differential activation of the influenza virus polymerase via template RNA binding

  1995cited by this paper
• NMRPipe: A multidimensional spectral processing system based on UNIX pipes

  1995cited by this paper
• Monoclonal antibodies against influenza virus PB2 and NP polypeptides interfere with the initiation step of viral mRNA synthesis in vitro

  1994cited by this paper
• Evaluation of the genetic stability of the temperature-sensitive PB2 gene mutation of the influenza A/Ann Arbor/6/60 cold-adapted vaccine virus

  1994cited by this paper
• Main-chain bond lengths and bond angles in protein structures.

  1993cited by this paper
• Determination of influenza virus proteins required for genome replication

  1990cited by this paper
• Complex formation between influenza virus polymerase proteins expressed in Xenopus oocytes

  1989cited by this paper
• Two of the three influenza viral polymerase proteins expressed by using baculovirus vectors form a complex in insect cells

  1987cited by this paper
• A unique cap(m7GpppXm)-dependent influenza virion endonuclease cleaves capped RNAs to generate the primers that initiate viral RNA transcription.

  1981cited by this paper
• Structural Basis for the Transition from Initiation to Elongation Transcription in T 7 RNA Polymerase

  year unknowncited by this paper

• Dual roles of USP39 in stabilizing PB2 and orchestrating ribonucleoprotein assembly drive H5 influenza virus replication and pathogenicity.

  2026cites this paper
• Chicken Pulmonary MicroRNAs Targeting the PB2 (Segment 1) of Avian Influenza Virus

  2025cites this paper
• Engineered interaction elements enable enhanced multi-enzyme assembly and cascade biocatalysis for indigo synthesis.

  2025cites this paper
• Antiviral strategies against influenza virus: an update on approved and innovative therapeutic approaches

  2025cites this paper
• Heterodimeric protein entangling motifs: systematic discovery, feature analysis, and topology engineering

  2025cites this paper
• An epidemiologic surveillance study based on wastewater and respiratory specimens reveals influenza a virus prevalence and mutations in Taiyuan, China during 2023–2024

  2024cites this paper
• Consortium of ‘consistent amino acid substitutions’ on influenza A (H1N1) viral proteins emerged at specific stages of viral infection: a big data analysis

  2024cites this paper
• Analysis of seasonal H3N2 influenza virus epidemic characteristics and whole genome features in Jining City from 2018 to 2023

  2024cites this paper
• BAG6 inhibits influenza A virus replication by inducing viral polymerase subunit PB2 degradation and perturbing RdRp complex assembly

  2024cites this paper
• Cryptic proteins translated from deletion-containing viral genomes dramatically expand the influenza virus proteome

  2024influential citation
• Advanced fluorescence microscopy in respiratory virus cell biology.

  2023cites this paper
• Recent Advances in DNA Nanotechnology-Enabled Biosensors for Virus Detection

  2023cites this paper
• A single antiviral for a triple epidemic: is it possible?

  2023cites this paper
• Targeting Metalloenzymes: The “Achilles’ Heel” of Viruses and Parasites

  2023cites this paper
• Cryptic proteins translated from deletion-containing viral genomes dramatically expand the influenza virus proteome

  2023influential citation
• The Effect of Mice Adaptation Process on the Pathogenicity of Influenza A/South Africa/3626/2013 (H1N1)pdm09 Model Strain

  2023influential citation
• Exploring new antiviral targets for influenza and COVID-19: Mapping promising hot spots in viral RNA polymerases

  2022cites this paper
• A Review and Meta-Analysis of Influenza Interactome Studies

  2022cites this paper
• Emerging antiviral therapies and drugs for the treatment of influenza

  2022cites this paper
• The ubiquitination landscape of the In uenza A Virus polymerase

  2022cites this paper
• Understanding the Variability of Certain Biological Properties of H1N1pdm09 Influenza Viruses

  2022cites this paper
• Pre-existing immunity to influenza viruses through infection and/or vaccination leads to viral mutational signatures associated with unique immune responses during a subsequent infection

  2022cites this paper
• The Influenza Virus RNA-Polymerase and the Host RNA-Polymerase II: RPB4 Is Targeted by a PB2 Domain That Is Involved in Viral Transcription

  2022cites this paper
• The ubiquitination landscape of the influenza A virus polymerase

  2022cites this paper
• Contemporary medicinal chemistry strategies for the discovery and optimization of influenza inhibitors targeting vRNP constituent proteins

  2021cites this paper
• Evolution of KIPPIS as a versatile platform for evaluating intracellularly functional peptide aptamers

  2021cites this paper
• A paucigranulocytic asthma host environment promotes the emergence of virulent influenza viral variants

  2021influential citation
• Molecular Docking and Virtual Screening of an Influenza Virus Inhibitor That Disrupts Protein–Protein Interactions

  2021influential citation
• Potent antiviral activity of the extract of Elaeocarpus sylvestris against influenza A virus in vitro and in vivo.

  2021cites this paper
• Prediction and characterization of influenza virus polymerase inhibitors through blind docking and ligand based virtual screening

  2021cites this paper
• Thrombopoietin receptor‐based protein–protein interaction screening (THROPPIS)

  2021cites this paper
• Virus Adaptation Following Experimental Infection of Chickens with a Domestic Duck Low Pathogenic Avian Influenza Isolate from the 2017 USA H7N9 Outbreak Identifies Polymorphic Mutations in Multiple Gene Segments

  2021influential citation
• Regulation of RNA Stability Through RNA Modification

  2021cites this paper
• Anti-Influenza Drug Discovery and Development: Targeting the Virus and Its Host by All Possible Means.

  2021cites this paper
• Synthesis and In Vitro Evaluation of C-7 and C-8 Luteolin Derivatives as Influenza Endonuclease Inhibitors

  2021cites this paper
• Visualization of the Interfacial Electrostatic Complementarity: A Method for Analysis of Protein-Protein Interaction Based on Ab Initio Quantum Chemical Calculations.

  2021cites this paper
• Toward a PA-Inhibitor Complex Crystal System: Influenza Polymerase Acidic Protein Fusion Constructs and Protein Expression

  2020cites this paper
• Inhibition of Influenza Virus Polymerase by Interfering with Its Protein–Protein Interactions

  2020cites this paper
• Impaired Nuclear Export of the Ribonucleoprotein Complex and Virus-Induced Cytotoxicity Combine to Restrict Propagation of the A/Duck/Malaysia/02/2001 (H9N2) Virus in Human Airway Cells

  2020cites this paper
• Structure and Function of Influenza Polymerase.

  2020influential citation
• Influenza A virus utilizes noncanonical cap-snatching to diversify its mRNA/ncRNA

  2020cites this paper
• In silico structure-based design of enhanced peptide inhibitors targeting RNA polymerase PAN-PB1C interaction

  2019cites this paper
• Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016

  2019cites this paper
• Potent and broad‐spectrum cycloheptathiophene‐3‐carboxamide compounds that target the PA‐PB1 interaction of influenza virus RNA polymerase and possess a high barrier to drug resistance

  2019influential citation
• A Review of Strategic Immune Evasion by Influenza Virus and Antiviral Response of Interferon

  2019cites this paper
• Novel antiviral drug discovery strategies to tackle drug-resistant mutants of influenza virus strains

  2019influential citation
• Identifying genetic determinants of complex phenotypes from whole genome sequence data

  2019cites this paper
• Structure–Function Relationship of Negative-Stranded Viral RNA Polymerases

  2019cites this paper
• Characterization of the PB2 Cap Binding Domain Accelerates Inhibitor Design

  2018cites this paper
• Influenza A virus polymerase: an attractive target for next-generation anti-influenza therapeutics.

  2018cites this paper
• Inhibitors of Influenza A Virus Polymerase.

  2018cites this paper
• Structure and Function of Influenza Virus Ribonucleoprotein.

  2018cites this paper
• Amino acid substitutions affecting aspartic acid 605 and valine 606 decrease the interaction strength between the influenza virus RNA polymerase PB2 '627' domain and the viral nucleoprotein

  2018cites this paper
• Evolutionary Targeted Discovery of Influenza A Virus Replication Inhibitors

  2018cites this paper
• Genetic and codon usage bias analyses of polymerase genes of equine influenza virus and its relation to evolution

  2017cites this paper
• Phylogenetic Analysis and Functional Characterization of the Influenza A H5N1 PB2 Gene

  2017cites this paper
• Identifying the genetic determinants of particular phenotypes in microbial genomes with very small training sets

  2017cites this paper
• Structure-based drug discovery for combating influenza virus by targeting the PA–PB1 interaction

  2017cites this paper
• Citation for published version: Hershna Patel and Andreas Kukol, 'Evolutionary conservation of influenza A PB2 sequences reveals potential target sites

  2017cites this paper
• Truncated forms of the PA protein containing only the C-terminal domains are associated with the RNP complex within H1N1 influenza virus particles.

  2017cites this paper
• Genetic variation and co-evolutionary relationship of RNA polymerase complex segments in influenza A viruses.

  2017cites this paper
• Truncated forms of the PA protein containing only the C-terminal domains are associated with the ribonucleoprotein complex within H 1 N 1 influenza virus particles

  2017cites this paper
• Biochemical characterization of recombinant influenza A polymerase heterotrimer complex: Polymerase activity and mechanisms of action of nucleotide analogs

  2017cites this paper
• Biologie structurale de complexes protéine:ARN

  2017cites this paper
• Structural insights into RNA synthesis by the influenza virus transcription-replication machine.

  2017cites this paper
• Progress of small molecular inhibitors in the development of anti-influenza virus agents

  2017cites this paper
• Effects of PB1-F2 and PA-X on the pathogenicity of H1N1 influenza virus

  2017cites this paper
• Evolutionary conservation of influenza A PB2 sequences reveals potential target sites for small molecule inhibitors.

  2017cites this paper
• Interferon induction by paramyxoviruses : investigations into specific RNA:protein interactions

  2017cites this paper
• Rapid acquisition of polymorphic virulence markers during adaptation of highly pathogenic avian influenza H5N8 virus in the mouse

  2017cites this paper
• Identifying genetic determinants of complex phenotypes from whole genome sequence data andvery small training sets

  2017cites this paper
• Caractérisation Structurale et Biochimique de la Nucléoprotéine des virus grippaux de type A, B et D

  2017cites this paper
• The Influenza Virus Polymerase Complex: An Update on Its Structure, Functions, and Significance for Antiviral Drug Design

  2016cites this paper
• Recherche de signaux d'empaquetage spécifiques du génome des virus influenza A

  2016cites this paper
• Identification of a small-molecule inhibitor of influenza virus via disrupting the subunits interaction of the viral polymerase.

  2016cites this paper
• Screening for Novel Small-Molecule Inhibitors Targeting the Assembly of Influenza Virus Polymerase Complex by a Bimolecular Luminescence Complementation-Based Reporter System

  2016cites this paper
• Peptide-Mediated Interference of PB2-eIF4G1 Interaction Inhibits Influenza A Viruses' Replication in Vitro and in Vivo.

  2016cites this paper
• CHARACTERIZATION OF INFLUENZA NUCLEOPROTEIN BODY DOMAIN AS ANTIVIRAL TARGET

  2016influential citation
• The PB1 segment of an influenza A virus H1N1 2009pdm isolate enhances the replication efficiency of specific influenza vaccine strains in cell culture and embryonated eggs.

  2016cites this paper
• Tracking the Evolution of Polymerase Genes of Influenza A Viruses during Interspecies Transmission between Avian and Swine Hosts

  2016cites this paper
• A molecular modelling approach to understand the effect of co-evolutionary mutations (V344M, I354L) identified in the PB2 subunit of influenza A 2009 pandemic H1N1 virus on m7GTP ligand binding.

  2016cites this paper
• Influenza virus RNA polymerase: insights into the mechanisms of viral RNA synthesis

  2016cites this paper
• Identification of a novel small-molecule compound targeting the influenza A virus polymerase PB1-PB2 interface

  2016cites this paper
• mRNA capping: biological functions and applications

  2016cites this paper
• Influenza Virus-specific CD8+ T Cells : -longevity, cross-reactivity and viral evasion-

  2016cites this paper
• Structural characterization of recombinant IAV polymerase reveals a stable complex between viral PA-PB1 heterodimer and host RanBP5

  2016influential citation
• Polycistronic Expression of the Influenza A Virus RNA-Dependent RNA Polymerase by Using the Thosea asigna Virus 2A-Like Self-Processing Sequence

  2016cites this paper
• Characterization of pathogenicity factors of a lethal influenza A(H1N1)09 pandemic viral isolate

  2016cites this paper
• Molecular Determinants of Virulence and Stability of a Reporter-Expressing H5N1 Influenza A Virus

  2015cites this paper
• Evidence for a Novel Mechanism of Influenza Virus-Induced Type I Interferon Expression by a Defective RNA-Encoded Protein

  2015influential citation
• Cryo-EM structure of influenza virus RNA polymerase complex at 4.3 Å resolution.

  2015cites this paper
• Influenza virus polymerase: Functions on host range, inhibition of cellular response to infection and pathogenicity.

  2015cites this paper
• Identification of a Novel Viral Protein Expressed from the PB2 Segment of Influenza A Virus

  2015cites this paper
• PB2 segment promotes high-pathogenicity of H5N1 avian influenza viruses in mice

  2015cites this paper
• Learning from structure-based drug design and new antivirals targeting the ribonucleoprotein complex for the treatment of influenza

  2015cites this paper
• Glycine at Position 622 in PB1 Contributes to the Virulence of H5N1 Avian Influenza Virus in Mice

  2015influential citation
• Conservational Analysis of Influenza A Virus RNA-dependent RNA Polymerase

  2015cites this paper
• New small-molecule drug design strategies for fighting resistant influenza A

  2015cites this paper
• Nasal vaccination using novel mucosal adjuvants - with main focus on influenza A virus

  2015cites this paper
• Development of Glycan Based Diagnostics to Detect Pathogens

  2015cites this paper