Structure of a bacterial ferredoxin.

Abstract The structure of the bacterial ferredoxin from Peptococcus aerogenes (54 amino acids) has been determined at 2.8 A resolution. The molecule is a prolate ellipsoid with approximate dimension of 22 x 27A. The iron and sulfur atoms are in two complexes 12 A apart with 4 iron, 4 inorganic sulfur, and 4 cysteine sulfur atoms in each. Cys 8, 11, 14, and 45 are coordinated to iron atoms in one complex and Cys 18, 35, 38, and 41 are coordinated to iron in the other. The two tyrosine groups present in the molecule are oriented in a similar way with respect to the complexes: Tyr 2 is parallel to and apparently in contact with one face of one complex and Tyr 28 is oriented similarly with respect to the other. Both tyrosine rings have an edge exposed to the solvent. The structure was solved with three isomorphous derivatives, at first used independently but later combined, to determine phases for the native data. Refinement of the structure has been initiated with 2 A resolution data.

• Publication year

  1973

• Venue

  Journal of Biological Chemistry

• Publication date

  1973-06-10

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(19)43829-5PMID 4708097
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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