Immunomodulation and signaling mechanism of Lactobacillus rhamnosus GG and its components on porcine intestinal epithelial cells stimulated by lipopolysaccharide.

BACKGROUND/PURPOSE This study aimed to evaluate the immunomodulatory effects and signaling mechanisms of Lactobacillus rhamnosus GG (LGG) and its components [surface-layer protein (SLP), DNA, exopolysaccharides, and CpG oligodeoxynucleotides] on lipopolysaccharide (LPS)-stimulated porcine intestinal epithelial cell (IEC) IPEC-J2. METHODS The mRNA expressions of inflammatory cytokines and Toll-like receptors (TLRs) were measured by quantitative real-time polymerase chain reaction. Activation of mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling was detected by western blot and immunofluorescence. RESULTS Pretreatment of IPEC-J2 cells with LGG, SLP, or exopolysaccharides significantly alleviated LPS-induced inflammatory cytokines and TLR activation at mRNA level. LGG, SLP, and exopolysaccharides also attenuated LPS-induced MAPK and NF-κB signaling activations. CpG oligodeoxynucleotides significantly increased the interleukin 12, tumor necrosis factor α, and TLR9 mRNA levels and enhanced NF-κB signaling activation in LPS-stimulated cells. CONCLUSION LGG had immunomodulatory effects on LPS-induced porcine IECs by modulating TLR expressions and inhibiting MAPK and NF-κB signaling to decrease inflammatory cytokine expressions. Components of LGG exerted immunomodulatory effects on porcine IECs, especially immunostimulatory CpG oligodeoxynucleotides.

• Publication year

  2017

• Venue

  Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi

• Publication date

  2017-10-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.jmii.2015.05.002PMID 26055689
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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