Structural Basis for Species-specific Differences in the Phosphorylation of Na,K-ATPase by Protein Kinase C (*)

There is considerable evidence that protein kinases play a role in regulation of the activity of the Na,K-ATPase, but the characteristics of direct kinase phosphorylation of Na,K-ATPase subunits are still not well understood. There are 36 sites that could qualify as protein kinase C motifs in rat α1. Here we have used protein fragmentation with trypsin to localize the site of phosphorylation of the rat Na,K-ATPase α1 subunit to within the first 32 amino acids of the N terminus and then used direct sequencing of the phosphorylated protein to determine which of two candidate serine residues was modified. The result was that at most 25% of the P was found on Ser-11, a site that is well conserved in Na,K-ATPase α1 subunits. The remaining 75% or more of the P was found on Ser-18, a site that is absent in many Na,K-ATPase α subunit sequences. This accounts for the observation that dog and pig α1 subunits can be phosphorylated by protein kinase C only to much lower levels than can rat α1. It is also likely to be relevant to other known species-specific effects of protein kinase C on Na,K-ATPase.

• Publication year

  1995

• Venue

  Journal of Biological Chemistry

• Publication date

  1995-06-09

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/JBC.270.23.14072PMID 7775468
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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