Tagging a T. brucei RRNA locus improves stable transfection efficiency and circumvents inducible expression position effects.

No abstract is available for this paper.

• Publication year

  2005

• Venue

  Molecular and biochemical parasitology (Print)

• Publication date

  2005-12-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/J.MOLBIOPARA.2005.08.009PMID 16182389PMCID PMC3833055
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2005cited by this paper
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  2004cited by this paper
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  2002cited by this paper
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  2001cited by this paper
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  2000cited by this paper
• Structural basis of gene regulation by the tetracycline inducible Tet repressor–operator system

  2000cited by this paper
• Double-stranded RNA induces mRNA degradation in Trypanosoma brucei.

  1998cited by this paper
• Trypanosoma brucei

  1998influential reference
• The PARP promoter of Trypanosoma brucei is developmentally regulated in a chromosomal context.

  1996cited by this paper
• Specific binding of proteins to the noncoding strand of a crucial element of the variant surface glycoprotein, procyclin, and ribosomal promoters of trypanosoma brucei

  1995cited by this paper
• Inducible gene expression in trypanosomes mediated by a prokaryotic repressor.

  1995cited by this paper
• Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination.

  1991cited by this paper
• Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers.

  1989influential reference
• Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to domain VII of other eukaryotic 28S rRNAs.

  1986cited by this paper

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