Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells

Xavier Pichon,Amandine Bastide,Adham Safieddine,Racha Chouaib,Aubin Samacoits,E. Basyuk,M. Peter,F. Mueller,E. Bertrand

Published 2016 in Journal of Cell Biology

ABSTRACT

Pichon et al. describe a method to visualize translation of single endogenous mRNPs in live cells and provide evidence for specialized translation factories, as well as measurements of translation elongation rate, ribosome loading, and movements of single polysomes.

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