Gelatin-Based Hydrogels Promote Chondrogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells In Vitro

Due to the weak regeneration potential of cartilage, there is a high clinical incidence of articular joint disease, leading to a strong demand for cartilaginous tissue surrogates. The aim of this study was to evaluate a gelatin-based hydrogel for its suitability to support chondrogenic differentiation of human mesenchymal stem cells. Gelatin-based hydrogels are biodegradable, show high biocompatibility, and offer possibilities to introduce functional groups and/or ligands. In order to prove their chondrogenesis-supporting potential, a hydrogel film was developed and compared with standard cell culture polystyrene regarding the differentiation behavior of human mesenchymal stem cells. Cellular basis for this study were human adipose tissue-derived mesenchymal stem cells, which exhibit differentiation potential along the adipogenic, osteogenic and chondrogenic lineage. The results obtained show a promotive effect of gelatin-based hydrogels on chondrogenic differentiation of mesenchymal stem cells in vitro and therefore encourage subsequent in vivo studies.

• Publication year

  2014

• Venue

  Materials

• Publication date

  2014-02-01

• Fields of study

  Medicine, Materials Science, Engineering

• Identifiers
  DOI 10.3390/ma7021342PMID 28788517PMCID 5453082
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Introduction to Biomaterials Science

  2020cited by this paper
• R: A language and environment for statistical computing.

  2014cited by this paper
• In Vivo Differentiation of Undifferentiated Human Adipose Tissue-Derived Mesenchymal Stem Cells in Critical-Sized Calvarial Bone Defects

  2014cited by this paper
• Immunocompatibility evaluation of hydrogel-coated polyimide implants for applications in regenerative medicine.

  2014cited by this paper
• Adipose-derived stromal/stem cells

  2013cited by this paper
• Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT).

  2013cited by this paper
• The meaning, the sense and the significance: translating the science of mesenchymal stem cells into medicine

  2013cited by this paper
• Gelatin-based nanoparticles as drug and gene delivery systems: reviewing three decades of research.

  2013cited by this paper
• A comparison of tissue-engineered bone from adipose-derived stem cell with autogenous bone repair in maxillary alveolar cleft model in dogs.

  2013cited by this paper
• Influence of solvent composition on the performance of carbodiimide cross-linked gelatin carriers for retinal sheet delivery

  2013cited by this paper
• Articular cartilage-derived cells hold a strong osteogenic differentiation potential in comparison to mesenchymal stem cells in vitro.

  2013cited by this paper
• Modification of human cancellous bone using Thai silk fibroin and gelatin for enhanced osteoconductive potential

  2013cited by this paper
• Long-term detection of human adipose-derived mesenchymal stem cells after intraarticular injection in SCID mice.

  2013cited by this paper
• Critical size bone defect reconstruction by an autologous 3D osteogenic-like tissue derived from differentiated adipose MSCs.

  2013cited by this paper
• Preparation, characterization, and in vitro enzymatic degradation of chitosan-gelatine hydrogel scaffolds as potential biomaterials.

  2012cited by this paper
• Molecular and biophysical mechanisms regulating hypertrophic differentiation in chondrocytes and mesenchymal stem cells.

  2012cited by this paper
• Perivascular Stem Cells: A Prospectively Purified Mesenchymal Stem Cell Population for Bone Tissue Engineering

  2012cited by this paper
• Biomaterials Science: An Introduction to Materials in Medicine, 3rd ed., Chapter I.1.4 Finite Element Analysis in Biomechanics

  2012cited by this paper
• Adipose-Derived Stem Cells Combined with a Demineralized Cancellous Bone Substrate for Bone Regeneration

  2012cited by this paper
• The elusive nature and function of mesenchymal stem cells

  2011cited by this paper
• Hydrogel design for cartilage tissue engineering: a case study with hyaluronic acid.

  2011cited by this paper
• Concise Review: Adipose‐Derived Stromal Cells for Skeletal Regenerative Medicine

  2011cited by this paper
• Unraveling the transcriptional regulatory machinery in chondrogenesis

  2011cited by this paper
• Biopolymer-based hydrogels as scaffolds for tissue engineering applications: a review.

  2011cited by this paper
• Local transplantation of human multipotent adipose-derived stem cells accelerates fracture healing via enhanced osteogenesis and angiogenesis

  2010cited by this paper
• In Vitro Proliferation and Chondrogenic Differentiation of Rat Bone Marrow Stem Cells Cultured with Gelatin Hydrogel Microspheres for TGF-β1 Release

  2010cited by this paper
• Hydrogel Network Formation Revised: High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance as a Powerful Tool for Measuring Absolute Hydrogel Cross-Link Efficiencies

  2010cited by this paper
• Posttranslational modifications of collagens as targets of hypoxia and Hif‐1α in endochondral bone development

  2010cited by this paper
• Mesenchymal and haematopoietic stem cells form a unique bone marrow niche

  2010cited by this paper
• Three-dimensional cultures of osteogenic and chondrogenic cells: a tissue engineering approach to mimic bone and cartilage in vitro.

  2009cited by this paper
• In vitro model of mesenchymal condensation during chondrogenic development.

  2009cited by this paper
• Novel maxillary reconstruction with ectopic bone formation by GMP adipose stem cells.

  2009cited by this paper
• A New Approach for Adipose Tissue Regeneration Based on Human Mesenchymal Stem Cells in Contact to Hydrogels—an In Vitro Study

  2009cited by this paper
• In vitro generation of an osteochondral construct using injectable hydrogel composites encapsulating rabbit marrow mesenchymal stem cells.

  2009cited by this paper
• Technology Insight: adult mesenchymal stem cells for osteoarthritis therapy

  2008cited by this paper
• Morphogenetic and Regulatory Mechanisms During Developmental Chondrogenesis: New Paradigms for Cartilage Tissue Engineering

  2008cited by this paper
• Cellular methods in cartilage research: primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells.

  2008influential reference
• Inverse H‐C ex situ HRMAS NMR experiments for solid‐phase peptide synthesis

  2008cited by this paper
• Self-renewing osteoprogenitors in bone marrow sinusoids can organize a hematopoietic microenvironment.

  2007cited by this paper
• Fetal Growth Plate

  2007cited by this paper
• Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement.

  2006cited by this paper
• Preparation and surface activity of gelatin derivative surfactants

  2006cited by this paper
• Hypoxia induces chondrocyte-specific gene expression in mesenchymal cells in association with transcriptional activation of Sox9.

  2005influential reference
• Transcriptional control of chondrocyte fate and differentiation.

  2005cited by this paper
• Distinct roles of Sox5, Sox6, and Sox9 in different stages of chondrogenic differentiation

  2005influential reference
• Autologous stem cells (adipose) and fibrin glue used to treat widespread traumatic calvarial defects: case report.

  2004cited by this paper
• Synthesis of New Polymethyloxazoline Hydrogels by the “Macroinitiator” Method

  2003cited by this paper
• Primer3 on the WWW for general users and for biologist programmers.

  2000cited by this paper
• ENHANCED RESOLUTION IN MAS NMR FOR COMBINATORIAL CHEMISTRY

  1997cited by this paper
• Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1

  1996cited by this paper
• Hypoxia regulates vascular endothelial growth factor gene expression in endothelial cells. Identification of a 5' enhancer.

  1995cited by this paper
• Transcriptional Regulation of the Rat Vascular Endothelial Growth Factor Gene by Hypoxia (*)

  1995cited by this paper
• The Science and technology of gelatin

  1977cited by this paper
• Surface staining of cartilage by Alcian Blue, with reference to the role of microscopic dye aggregates in histological staining

  1974influential reference
• Osteogenesis in transplants of bone marrow cells.

  1966cited by this paper
• Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian blue in salt solutions

  1965influential reference

• Shaping the mechanical properties of a gelatin hydrogel interface via amination.

  2025cites this paper
• Biopolymer hydrogels in biomedicine: Bridging chemistry, biology, and clinical translation.

  2025cites this paper
• OAlgMA/GelMA/CaCl2 multinetwork hydrogel scaffolds with enhanced mechanical properties for cartilage injury repair

  2025cites this paper
• Hydrogel Films in Biomedical Applications: Fabrication, Properties and Therapeutic Potential

  2025cites this paper
• Gelatin‐Mediated Vascular Self‐Assembly via a YAP‐MMP Signaling Axis

  2024cites this paper
• Resembling mesenchymal stem cell microenvironment with growth factors loaded synthetic coral matrix for bone tissue engineering

  2024cites this paper
• Histological evaluation of the regenerative potential of a novel photocrosslinkable gelatin-treated dentin matrix hydrogel in direct pulp capping: an animal study

  2024cites this paper
• Fabrication of a three-dimensional scaffold-free trachea with horseshoe-shaped hyaline cartilage.

  2024cites this paper
• Bioengineered cartilaginous grafts for repairing segmental mandibular defects

  2024cites this paper
• A review of bioengineering techniques applied to breast tissue: Mechanical properties, tissue engineering and finite element analysis

  2023cites this paper
• 3D Spheroid Cultivation Alters the Extent and Progression of Osteogenic Differentiation of Mesenchymal Stem/Stromal Cells Compared to 2D Cultivation

  2023cites this paper
• Adhesive hydrogels in osteoarthritis: from design to application

  2023cites this paper
• Electrical Stimuli-Responsive Gelatin/Shellac Gel Blends for Use as Controllable Transdermal Delivery Patches

  2023cites this paper
• In Vitro Chondrogenic Differentiation of Human Adipose-Derived Stem Cells by Diacerein

  2023cites this paper
• Increasing Collagen to Bioink Drives Mesenchymal Stromal Cells-Chondrogenesis from Hyaline to Calcified Layers

  2023cites this paper
• Chondrogenic Potential of Human Adipose-Derived Mesenchymal Stromal Cells in Steam Sterilized Gelatin/Chitosan/Polyvinyl Alcohol Hydrogels

  2023cites this paper
• Effect of curcumin-containing nanofibrous gelatin-hydroxyapatite scaffold on proliferation and early osteogenic differentiation of dental pulp stem cells.

  2023cites this paper
• Revolutionizing bone regeneration: advanced biomaterials for healing compromised bone defects

  2023cites this paper
• O-alg-THAM/gel hydrogels functionalized with engineered microspheres based on mesenchymal stem cell secretion recruit endogenous stem cells for cartilage repair

  2023cites this paper
• Anatomy, molecular structures, and hyaluronic acid - Gelatin injectable hydrogels as a therapeutic alternative for hyaline cartilage recovery: A review.

  2023cites this paper
• Click chemistry for 3D bioprinting.

  2023cites this paper
• Fabrication of a Cell-Friendly Poly(dimethylsiloxane) Culture Surface via Polydopamine Coating

  2022cites this paper
• Three-Dimensional Digital Light-Processing Bioprinting Using Silk Fibroin-Based Bio-Ink: Recent Advancements in Biomedical Applications

  2022cites this paper
• The Role of Extracellular Matrix and Hydrogels in Mesenchymal Stem Cell Chondrogenesis and Cartilage Regeneration

  2022cites this paper
• 77890Effect of gelatinous spongy scaffold containing nano-hydroxyapatite on the induction of odontogenic activity of dental pulp stem cells

  2022cites this paper
• Novel nanocomposite scaffold based on Gelatin/PLGA-PEG-PLGA hydrogels embedded with TGF-β1 for chondrogenic differentiation of human dental pulp stem cells in vitro.

  2022cites this paper
• The Effects of Tgfb1 and Csf3 on Chondrogenic Differentiation of iPS Cells in 2D and 3D Culture Environment

  2021cites this paper
• A versatile synthetic route towards gelatin-silica hybrids and magnetic composite colloidal nanoparticles

  2021cites this paper
• In Vivo Studies of Mesenchymal Stem Cells in the Treatment of Meniscus Injury

  2021cites this paper
• Effect of Polymeric Matrix Stiffness on Osteogenic Differentiation of Mesenchymal Stem/Progenitor Cells: Concise Review

  2021cites this paper
• Development of alginate dialdehyde-gelatin based bioink with methylcellulose for improving printability.

  2021cites this paper
• Mini review: Biomaterials in repair and regeneration of nerve in a volumetric muscle loss

  2021cites this paper
• 3D printed gelatin/hydroxyapatite scaffolds for stem cell chondrogenic differentiation and articular cartilage repair.

  2021cites this paper
• Hybrid Bioprinting of Chondrogenically Induced Human Mesenchymal Stem Cell Spheroids

  2020cites this paper
• Biomimetic gradient scaffold of collagen–hydroxyapatite for osteochondral regeneration

  2020cites this paper
• Tricomponent thermoresponsive polymers based on an amine-containing monomer with tuneable hydrophobicity: Effect of composition

  2020cites this paper
• Effect of electrical stimulation on chondrogenic differentiation of mesenchymal stem cells cultured in hyaluronic acid - Gelatin injectable hydrogels.

  2020cites this paper
• Silk Fibroin Bioinks for Digital Light Processing (DLP) 3D Bioprinting.

  2020cites this paper
• Energy metabolic configuration of human adipose tissue- derived cells and its changes due to in vitro culture with osteogenic and adipogenic differentiation

  2020cites this paper
• A tri-component knee plug for the 3rd generation of autologous chondrocyte implantation

  2020cites this paper
• Hydrogels and Dentin–Pulp Complex Regeneration: From the Benchtop to Clinical Translation

  2020cites this paper
• Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

  2020cites this paper
• Gelatin; Switch back to Halal: A Mini-Review

  2019cites this paper
• Natural and Synthetic Materials in Regenerative Medicine: Progress Over the Past Five Years

  2019cites this paper
• The effect of electric fields on hyaline cartilage: an in vitroand in silicostudy

  2019cites this paper
• Gelatin-Based Hydrogels

  2019cites this paper
• Magnetic Enhancement of Chondrogenic Differentiation of Mesenchymal Stem Cells.

  2019cites this paper
• The Effects of Leptin on the Proliferation and Differentiation of Primary Chondrocytes in Vitro and Cartilage Regeneration in Vivo.

  2019cites this paper
• Recent Advances in Biology and Medicine

  2019cites this paper
• Biological hyaluronic-acid based and chondroitin sulfate-based hybrids hydrogels with great potential for cartilage substitution and regeneration

  2019cites this paper
• A Scaffold-Free Allogeneic Construct From Adipose-Derived Stem Cells Regenerates an Osteochondral Defect in a Rabbit Model.

  2019cites this paper
• Fiber Reinforced Cartilage ECM Functionalized Bioinks for Functional Cartilage Tissue Engineering

  2019cites this paper
• Three-Dimensional-Bioprinted Dopamine-Based Matrix for Promoting Neural Regeneration.

  2018cites this paper
• Gelatin Methacryloyl Hydrogels in the Absence of a Crosslinker as 3D Glioblastoma Multiforme (GBM)-Mimetic Microenvironment.

  2018cites this paper
• Electrospinning of highly concentrated albumin patches by using auxiliary polymers for laser-assisted vascular anastomosis

  2018cites this paper
• Gelatin Endorses the Pluripotent Stem Cells from the Reprogrammed Fibroblasts of Adult Mice Differentiation into Subtypes of Neurons in the Feeder-Free Condition for a Long-Term

  2018cites this paper
• Cellulose hydrogel film for spheroid formation of human adipose-derived stemcells

  2018cites this paper
• Meeting the requirements of halal gelatin: A mini review

  2018cites this paper
• Three-Dimensional Bioprinting of Cartilage by the Use of Stem Cells: A Strategy to Improve Regeneration

  2018cites this paper
• Synthesis of a thin-layer gelatin nanofiber mat for cultivating retinal cell

  2018cites this paper
• Advances in stem cell therapy for cartilage regeneration in osteoarthritis

  2018cites this paper
• Recent Advances in Edible Polymer Based Hydrogels as a Sustainable Alternative to Conventional Polymers.

  2018cites this paper
• Hydrogels for Cartilage Regeneration, from Polysaccharides to Hybrids

  2017cites this paper
• Gelatin- and starch-based hydrogels. Part B: In vitro mesenchymal stem cell behavior on the hydrogels.

  2017cites this paper
• Scaffold-Free Tissue-Engineered Allogenic Adipose-Derived Stem Cells Promote Meniscus Healing.

  2017cites this paper
• Macroporous Hydrogel Scaffolds for Three-Dimensional Cell Culture and Tissue Engineering

  2017cites this paper
• Preparation and characterization of hybrid double network chitosan/poly(acrylic amide-acrylic acid) high toughness hydrogel through Al3+ crosslinking.

  2017cites this paper
• Regeneration of neurite-like cells from induced pluripotent stem cells in self-assembled hyaluronic acid-gelatin microhydrogel

  2016cites this paper
• Gelatin- and starch-based hydrogels. Part A: Hydrogel development, characterization and coating.

  2016cites this paper
• Injectable Polypeptide Hydrogels with Tunable Microenvironment for 3D Spreading and Chondrogenic Differentiation of Bone-Marrow-Derived Mesenchymal Stem Cells.

  2016cites this paper
• Cross-linkable polyethers as healing/sealing agents for self-healing of cementitious materials

  2016cites this paper
• Mise en œuvre d’un système de confinement et de délivrance moléculaire pour la production in situ de glucose au sein d’un hydrogel conçu pour l'ingénierie tissulaire

  2016cites this paper
• Mesenchymal cells condensation-inducible mesh scaffolds for cartilage tissue engineering.

  2016cites this paper
• Precise Tuning of Facile One-Pot Gelatin Methacryloyl (GelMA) Synthesis

  2016cites this paper
• Composite Hydrogels for Bone Regeneration

  2016cites this paper
• Photo-crosslinkable biopolymers targeting stem cell adhesion and proliferation: the case study of gelatin and starch-based IPNs

  2015cites this paper
• Proliferation and Differentiation Potential of Human Adipose-Derived Stem Cells Grown on Chitosan Hydrogel

  2015cites this paper
• Natural Biomaterials for Veterinary Regenerative Therapy

  2015cites this paper
• Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) with Gelatin Solvent

  2015cites this paper
• Cryogel-PCL combination scaffolds for bone tissue repair

  2015cites this paper
• Isolation and differentiation potential of human mesenchymal stem cells from adipose tissue harvested by water jet-assisted liposuction.

  2015cites this paper
• Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential

  2014cites this paper