An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues

BackgroundPhytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and modulating plant reactions targeted against pathogens and herbivores, as well as resistance to abiotic stress (drought, UV-irradiation and mechanical wounding). These factors induce dramatic changes in phytohormone biosynthesis and transport leading to rapid local and systemic stress responses. Understanding of underlying mechanisms is of principle interest for scientists working in various areas of plant biology. However, highly sensitive, precise and high-throughput methods for quantification of these phytohormones in small samples of plant tissues are still missing.ResultsHere we present an LC-MS/MS method for fast and highly sensitive determination of jasmonates, abscisic and salicylic acids. A single-step sample preparation procedure based on mixed-mode solid phase extraction was efficiently combined with essential improvements in mobile phase composition yielding higher efficiency of chromatographic separation and MS-sensitivity. This strategy resulted in dramatic increase in overall sensitivity, allowing successful determination of phytohormones in small (less than 50 mg of fresh weight) tissue samples. The method was completely validated in terms of analyte recovery, sensitivity, linearity and precision. Additionally, it was cross-validated with a well-established GC-MS-based procedure and its applicability to a variety of plant species and organs was verified.ConclusionThe method can be applied for the analyses of target phytohormones in small tissue samples obtained from any plant species and/or plant part relying on any commercially available (even less sensitive) tandem mass spectrometry instrumentation.

• Publication year

  2012

• Venue

  Plant Methods

• Publication date

  2012-11-22

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1186/1746-4811-8-47PMID 23173950PMCID 3573895
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• The assay was validated for recovery, sensitivity, linearity, and precision and cross-validated against a GC-MS-based procedure across multiple plant species and organs.

  Confidence 0.93

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewJake Seo (Twitter) (bmuzj8twwb) reviewmexicorea (qjvnbu8xg3) review
• The assay successfully quantified phytohormones from plant tissue inputs smaller than 50 mg fresh weight.

  Confidence 0.95

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewJake Seo (Twitter) (bmuzj8twwb) reviewmexicorea (qjvnbu8xg3) review
• Combining mixed-mode solid phase extraction with optimized mobile phase composition yielded a fast, highly sensitive UPLC-MS/MS assay for phytohormone quantification.

  Confidence 0.97

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewJake Seo (Twitter) (bmuzj8twwb) reviewmexicorea (qjvnbu8xg3) review

• gc-ms-based procedure

  reference methods

  A gas chromatography mass spectrometry workflow used as a reference method for comparison.

  Aliases: GC-MS

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewJake Seo (Twitter) (bmuzj8twwb) reviewmexicorea (qjvnbu8xg3) review
• method validation

  validation process

  Assessment of the assay using recovery, sensitivity, linearity, and precision measurements.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewJake Seo (Twitter) (bmuzj8twwb) reviewmexicorea (qjvnbu8xg3) review
• mixed-mode solid phase extraction

  sample preparation method

  A single-step sample cleanup approach that combines more than one retention mechanism before mass spectrometric analysis.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewJake Seo (Twitter) (bmuzj8twwb) reviewmexicorea (qjvnbu8xg3) review
• mobile phase composition

  chromatography condition

  The liquid-phase solvent mixture used during chromatographic separation and adjusted here for the assay.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewJake Seo (Twitter) (bmuzj8twwb) reviewmexicorea (qjvnbu8xg3) review
• phytohormones

  metabolites

  The plant hormone analytes targeted in the assay, including jasmonates, abscisic acid, and salicylic acid.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewJake Seo (Twitter) (bmuzj8twwb) reviewmexicorea (qjvnbu8xg3) review
• plant species and organs

  biological scope

  Different plant taxa and tissue types used to check the assay's applicability.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewJake Seo (Twitter) (bmuzj8twwb) reviewmexicorea (qjvnbu8xg3) review
• small tissue samples

  sample type

  Plant tissue inputs below about 50 mg fresh weight used to test the assay's performance.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewJake Seo (Twitter) (bmuzj8twwb) reviewmexicorea (qjvnbu8xg3) review
• uplc-ms/ms method

  analytical method

  An ultra-performance liquid chromatography tandem mass spectrometry assay used here for phytohormone quantification in plant tissues.

  Aliases: LC-MS/MS

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewJake Seo (Twitter) (bmuzj8twwb) reviewmexicorea (qjvnbu8xg3) review

• Ultrasensitive determination of jasmonic acid in plant tissues using high-performance liquid chromatography with fluorescence detection.

  2012cited by this paper
• A convenient method for simultaneous quantification of multiple phytohormones and metabolites: application in study of rice-bacterium interaction

  2012cited by this paper
• Repeated leaf wounding alters the colonization of Medicago truncatula roots by beneficial and pathogenic microorganisms.

  2012cited by this paper
• Cell-specific visualization of jasmonates in wounded tomato and Arabidopsis leaves using jasmonate-specific antibodies.

  2011cited by this paper
• Visualization of plant viral suppressor silencing activity in intact leaf lamina by quantitative fluorescent imaging

  2011cited by this paper
• SELECTION OF BUFFERS IN LC-MS/MS: AN OVERVIEW

  2011cited by this paper
• QuEChERS-based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC-MS/MS.

  2011influential reference
• Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography-electrospray tandem mass spectrometry and the evaluation of the matrix effect.

  2011cited by this paper
• Separation selectivity of some phenolic acids in RP HPLC systems with binary mobile phase comprised various modifiers

  2010cited by this paper
• A tomato enzyme synthesizes (+)-7-iso-jasmonoyl-l-isoleucine in wounded leaves

  2010cited by this paper
• Hormonal and transcriptional profiles highlight common and differential host responses to arbuscular mycorrhizal fungi and the regulation of the oxylipin pathway

  2010cited by this paper
• Determination of both jasmonic acid and methyl jasmonate in plant samples by liquid chromatography tandem mass spectrometry

  2010cited by this paper
• A rapid and sensitive method for the simultaneous analysis of aliphatic and polar molecules containing free carboxyl groups in plant extracts by LC-MS/MS

  2009cited by this paper
• (+)-7-iso-Jasmonoyl-L-isoleucine is the endogenous bioactive jasmonate.

  2009cited by this paper
• A rapid wound signal activates the systemic synthesis of bioactive jasmonates in Arabidopsis.

  2009cited by this paper
• The wound hormone jasmonate.

  2009cited by this paper
• Velocity Estimates for Signal Propagation Leading to Systemic Jasmonic Acid Accumulation in Wounded Arabidopsis*

  2009cited by this paper
• A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress

  2008cited by this paper
• Protocol: Streamline cloning of genes into binary vectors in Agrobacterium via the Gateway® TOPO vector system

  2008cited by this paper
• Spatial and Temporal Dynamics of Jasmonate Synthesis and Accumulation in Arabidopsis in Response to Wounding*

  2008influential reference
• Separation of Amadori peptides from their unmodified analogs by ion-pairing RP-HPLC with heptafluorobutyric acid as ion-pair reagent

  2008cited by this paper
• Distinct Roles of Jasmonates and Aldehydes in Plant-Defense Responses

  2008cited by this paper
• Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction.

  2008influential reference
• Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography-electrospray tandem mass spectrometry.

  2008cited by this paper
• HPLC for Pharmaceutical Scientists

  2007cited by this paper
• Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development.

  2007cited by this paper
• Hormone Profiling by LC-QToF-MS/MS in Dormant Macadamia integrifolia: Correlations with Abnormal Vertical Growth

  2007cited by this paper
• The wound response in tomato--role of jasmonic acid.

  2006cited by this paper
• Tailor-made composite functions as tools in model choice: the case of sigmoidal vs bi-linear growth profiles

  2006cited by this paper
• Oxylipin analysis methods.

  2006cited by this paper
• Simultaneous quantitative LC-ESI-MS/MS analyses of salicylic acid and jasmonic acid in crude extracts of Cucumis sativus under biotic stress.

  2006cited by this paper
• LC/MS: Theory, Instrumentation, and Applications to Small Molecules

  2006cited by this paper
• Diurnal variation of cytokinin, auxin and abscisic acid levels in tobacco leaves.

  2005cited by this paper
• Matrix effects: the Achilles heel of quantitative high-performance liquid chromatography-electrospray-tandem mass spectrometry.

  2005cited by this paper
• Simultaneous determination of multiple phytohormones in plant extracts by liquid chromatography-electrospray tandem mass spectrometry.

  2005influential reference
• Qualitätssicherung in der Analytischen Chemie

  2005cited by this paper
• The Oxylipin Signal Jasmonic Acid Is Activated by an Enzyme That Conjugates It to Isoleucine in Arabidopsis

  2004cited by this paper
• A method for profiling classes of plant hormones and their metabolites using liquid chromatography-electrospray ionization tandem mass spectrometry: an analysis of hormone regulation of thermodormancy of lettuce (Lactuca sativa L.) seeds.

  2003cited by this paper
• Jasmonate biosynthesis and the allene oxide cyclase family of Arabidopsis thaliana

  2003influential reference
• Allene oxide cyclase dependence of the wound response and vascular bundle-specific generation of jasmonates in tomato - amplification in wound signalling.

  2003cited by this paper
• Impaired Induction of the Jasmonate Pathway in the Rice Mutant hebiba1

  2003cited by this paper
• Direct analysis of abscisic acid in crude plant extracts by liquid chromatography--electrospray/tandem mass spectrometry.

  2002cited by this paper
• Octadecanoid and Jasmonate Signaling in Tomato (Lycopersicon esculentum Mill.) Leaves: Endogenous Jasmonates Do Not Induce Jasmonate Biosynthesis

  2000cited by this paper
• Analysis of synthetic isoleucine conjugates of cucurbic acid isomers by liquid chromatography

  1999cited by this paper
• Quantification of amino acid conjugates of jasmonic acid in rice leaves by high-performance liquid chromatography–turboionspray tandem mass spectrometry

  1998cited by this paper
• Ultrahigh-pressure reversed-phase liquid chromatography in packed capillary columns.

  1997cited by this paper
• Salicylic Acid Inhibits Synthesis of Proteinase Inhibitors in Tomato Leaves Induced by Systemin and Jasmonic Acid

  1995cited by this paper
• Hydroxylated jasmonic acid and related compounds from Botryodiplodia theobromae

  1991cited by this paper
• Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato.

  1989cited by this paper
• Synthesis of Jasmine Ketolactone

  1984cited by this paper
• Characterization of a prostaglandin-like metabolite of linolenic acid produced by a flaxseed extract

  1978influential reference

• Transcriptomic and hormonal dynamics in relation to adventitious rooting of two parental Petunia species highlight a coordinated activation of the jasmonate and auxin pathways and an important role of upper-shoot-derived auxin influx.

  2026cites this paper
• Metabolome and Transcriptome Profiling of Chicory Roots Provide Insights Into Laticifer Development and Specialized Metabolism

  2026cites this paper
• Plant wearable sensors: In-situ monitoring of pathogens and agricultural pollutants for precision agriculture

  2026cites this paper
• Jasmonic acid negatively regulates wheat grain filling by affecting assimilate transport and starch biosynthesis

  2026cites this paper
• Analyzing Plant Low-Molecular-Weight Polar Metabolites: A GC-MS Approach

  2026cites this paper
• Comparative Transcriptome and Hormonal Analysis Reveals the Mechanisms of Salt Tolerance in Rice

  2025cites this paper
• Advanced high-resolution tandem mass spectrometry for precise diterpenoid identification and spectral library development

  2025cites this paper
• Development and Validation of an LC–MS/MS Method for Quantifying Phytohormones Related to Tomato Shelf Life

  2025cites this paper
• Low-nitrate-inducible overexpression of PagTCP30.1 promotes root elongation in Populus alba × P. glandulosa (84K).

  2025cites this paper
• Advances in surface-enhanced Raman scattering applications for precision agriculture: monitoring plant health and crop quality

  2025cites this paper
• Root driven hormones and plant growth promoting rhizobacteria as natural biostimulants for sustainable crop growth

  2025cites this paper
• Integrated physiological, transcriptomic, and metabolic analysis reveals the effects of nanoplastics exposure on tea plants.

  2025cites this paper
• Enhanced Biochemical and Structural Defense in PGPR-Inoculated Sweet Basil Under Aphid Herbivory

  2025influential citation
• A Non-Specific Phytohormone Regulatory Network in Saccharina japonica Coordinates Growth and Environmental Adaptation

  2025cites this paper
• Formation and Regulation Mechanism of Ascorbic Acid in Sweet Pepper and Chili Pepper at Different Growth Stages

  2025cites this paper
• Exploring the biostimulant potential of Scenedesmus sp. grown in wastewater: impacts on plant growth and photosynthetic activity of lettuce.

  2025cites this paper
• Pleiotropic effects of barley BLADE-ON-PETIOLE gene Uniculme4 on plant architecture and the jasmonic acid pathway

  2025cites this paper
• Combined Physiology and Transcriptome Analyses Provide Insights into Malformed Fruit of Cocos nucifera L.

  2025cites this paper
• Influence of Plant Growth-Promoting Rhizobacteria (PGPR) Inoculation on Phenolic Content and Key Biosynthesis-Related Processes in Ocimum basilicum Under Spodoptera frugiperda Herbivory

  2025cites this paper
• Drought response of the maize plant-soil-microbiome system is influenced by plant size and presence of root hairs.

  2025cites this paper
• Multi-Omics Research Reveals the Effects of the ABA-Regulated Phenylpropanoid Biosynthesis Pathway on the UV-B Response in Rhododendron chrysanthum Pall.

  2025cites this paper
• Circadian-Mediated Regulation of Growth, Chloroplast Proteome, Targeted Metabolomics and Gene Regulatory Network in Spinacia oleracea Under Drought Stress

  2025cites this paper
• The rice cation/calcium exchanger OsCCX2 is involved in calcium signal clearance and osmotic tolerance.

  2025cites this paper
• Editing a gibberellin receptor gene improves yield and nitrogen fixation in soybean

  2025cites this paper
• The Endosperm-Specific Gene OsEnS-42 Regulates Seed Vigor and Grain Quality

  2025cites this paper
• Transcriptomics and trans-organellar complementation reveal limited signaling of 12-cis-oxo-phytodienoic acid during early wound response in Arabidopsis

  2025cites this paper
• Integration of Phenotypes, Phytohormones, and Transcriptomes to Elucidate the Mechanism Governing Early Physiological Abscission in Coconut Fruits (Cocos nucifera L.)

  2024cites this paper
• GH3 Gene Family Identification in Chinese White Pear (Pyrus bretschneideri) and the Functional Analysis of PbrGH3.5 in Fe Deficiency Responses in Tomato

  2024cites this paper
• The Transcription Factor BoNAC02 Enhances the Drought Resistance of Kale through an ABA-Dependent Pathway

  2024cites this paper
• Transcriptome and metabolome analyses reveal that GA3ox regulates the dwarf trait in mango (Mangifera indica L.)

  2024cites this paper
• Widespread production of plant growth-promoting hormones among marine bacteria and their impacts on the growth of a marine diatom

  2024cites this paper
• Genetic Transformation of Triticum dicoccum and Triticum aestivum with Genes of Jasmonate Biosynthesis Pathway Affects Growth and Productivity Characteristics

  2024cites this paper
• Methyl salicylate induces endogenous jasmonic acid and salicylic acid in 'Nam Dok Mai' mango to maintain postharvest ripening and quality.

  2024cites this paper
• PbbHLH137 interacts with PbGIF1 to regulate pear fruit development by promoting cell expansion to increase fruit size.

  2024cites this paper
• Molecular Mechanism of Exogenous ABA to Enhance UV-B Resistance in Rhododendron chrysanthum Pall. by Modulating Flavonoid Accumulation

  2024cites this paper
• Hypoxia reshapes Arabidopsis root architecture by integrating ERF-VII factor response and abscisic acid homoeostasis.

  2024cites this paper
• Decoding early stress signaling waves in living plants using nanosensor multiplexing

  2024cites this paper
• Bifunctional transcription factors SlERF.H5 and H7 activate cell wall and repress gibberellin biosynthesis genes in tomato via a conserved motif.

  2024cites this paper
• The plant specific cohesin subunit SYN4 contributes to 3D genome organization

  2024influential citation
• The cellular and molecular basis of the spur development in Impatiens uliginosa

  2024cites this paper
• Impact of cis-zeatin and lovastatin on antioxidant systems and growth parameters in Zea mays seedlings under phytohormonal crosstalks

  2024cites this paper
• Grain Chalkiness Is Decreased by Balancing the Synthesis of Protein and Starch in Hybrid Indica Rice Grains under Nitrogen Fertilization

  2024cites this paper
• Genome-wide identification of GA2ox genes family and analysis of PbrGA2ox1-mediated enhanced chlorophyll accumulation by promoting chloroplast development in pear

  2024cites this paper
• Importance of abscisic acid and zeatin nucleosides for the nitrate-induced cadmium hyperaccumulation in Populus roots

  2024cites this paper
• Phytohormones in a universe of regulatory metabolites: lessons from jasmonate

  2024cites this paper
• Overexpression of PbrGA2ox1 enhances pear drought tolerance through regulation of GA3-inhibited reactive oxygen species detoxification and abscisic acid signaling1

  2024cites this paper
• Modulating auxin response stabilizes tomato fruit set.

  2023cites this paper
• WHIRLY1 Acts Upstream of ABA-Related Reprogramming of Drought-Induced Gene Expression in Barley and Affects Stress-Related Histone Modifications

  2023cites this paper
• A novel chorismate mutase effector secreted from root-knot nematode Meloidogyne enterolobii manipulates plant immunity to promote parasitism1

  2023cites this paper
• Jarin-1, an inhibitor of JA-Ile biosynthesis in Arabidopsis thaliana, acts differently in other plant species

  2023cites this paper
• Endogenously Produced Jasmonates Affect Leaf Growth and Improve Osmotic Stress Tolerance in Emmer Wheat

  2023cites this paper
• Enhancing sustainability through microalgae cultivation in urban wastewater for biostimulant production and nutrient recovery.

  2023cites this paper
• A novel sustainable biomass-based fluorescent probe for sensitive detection of salicylic acid in rice.

  2023cites this paper
• Volatile Organic Compounds Emitted by the Biocontrol Agent Pythium oligandrum Contribute to Ginger Plant Growth and Disease Resistance

  2023cites this paper
• Heat stress induces a developmental shift from type-V to type-IV trichome dependent on jasmonate signaling in tomato

  2023cites this paper
• PbGIF1 promoting cell-proliferation in pear fruit is transcriptionally activated by PbRR1

  2023cites this paper
• Wearable electrochemical sensors for plant small-molecule detection.

  2023cites this paper
• Substrate space analysis of the bacterial proton-coupled oligopeptide transporter YdgR by cheminformatics.

  2023cites this paper
• 12-Oxophytodienoate Reductase Overexpression Compromises Tolerance to Botrytis cinerea in Hexaploid and Tetraploid Wheat

  2023cites this paper
• The dynamics of touch-responsive gene expression in cereals.

  2023cites this paper
• Phytohormone profiling in an evolutionary framework

  2023cites this paper
• Dynamics of Reactive Carbonyl Species in Pea Root Nodules in Response to Polyethylene Glycol (PEG)-Induced Osmotic Stress

  2022cites this paper
• Heat Stress Resistance Mechanisms of Two Cucumber Varieties from Different Regions

  2022cites this paper
• Phytochemical Analysis and Antioxidant Activity of Crocus speciosus Leaves

  2022cites this paper
• The Effect of Chitosan on Plant Physiology, Wound Response, and Fruit Quality of Tomato

  2022cites this paper
• High-throughput interspecies profiling of acidic plant hormones using miniaturised sample processing

  2022cites this paper
• Gain time to adapt: How sorghum acquires tolerance to salinity

  2022cites this paper
• Agar-based polyethylene glycol (PEG) infusion model for pea (Pisum sativum L.) — perspectives of translation to legume crop plants

  2022cites this paper
• MCU proteins dominate in vivo mitochondrial Ca2+ uptake in Arabidopsis roots

  2022cites this paper
• Characterization of Organellar-Specific ABA Responses during Environmental Stresses in Tobacco Cells and Arabidopsis Plants

  2022cites this paper
• Increasing SnRK1 activity with the AMPK activator A-769662 accelerates seed germination in rice.

  2022cites this paper
• Non-destructive Technologies for Plant Health Diagnosis

  2022cites this paper
• Interspecific Neighbor Stimulates Peanut Growth Through Modulating Root Endophytic Microbial Community Construction

  2022cites this paper
• ABA analogue produced by Bacillus marisflavi modulates the physiological response of Brassica juncea L. under drought stress

  2021cites this paper
• PSK signaling controls ABA homeostasis and signaling genes and 1 maintains shoot growth under osmotic stress

  2021cites this paper
• Transcriptome Analysis Reveals the Complex Molecular Mechanisms of Brassica napus–Sclerotinia sclerotiorum Interactions

  2021cites this paper
• Inhibition of gibberellin accumulation by water deficiency promotes fast and long-term ‘drought avoidance’ responses in tomato

  2021cites this paper
• Cold-priming causes dampening of oxylipin biosynthesis and signalling during the early cold- and light-triggering response of Arabidopsis thaliana

  2021cites this paper
• Influence of Climatic Conditions and Phenological Stages on Chemical Composition, Essential Oils and Anatomical Characteristics of Ajuga iva (L.) Schreb.

  2021influential citation
• Phytonutrients and Metabolism Changes in Topped Radish Root and Its Detached Leaves during 1 °C Cold Postharvest Storage

  2021cites this paper
• The jasmonate biosynthesis Gene OsOPR7 can mitigate salinity induced mitochondrial oxidative stress.

  2021cites this paper
• Comprehensive transcriptome profiling to identify genes involved in pistil abortion of Japanese apricot

  2021cites this paper
• A Plug-and-Play Type Field-Deployable Bio-Agent-Free Salicylic Acid Sensing System

  2021cites this paper
• Pinus pinaster Early Hormonal Defence Responses to Pinewood Nematode (Bursaphelenchus xylophilus) Infection

  2021cites this paper
• DNA hypomethylation in tetraploid rice potentiates stress-responsive gene expression for salt tolerance

  2021cites this paper
• PbEIL1 acts upstream of PbCysp1 to regulate ovule senescence in seedless pear

  2021cites this paper
• Quantitative analysis of seven plant hormones in Lotus japonicus using standard addition method

  2021cites this paper
• A Transcriptome Analysis Revealing the New Insight of Green Light on Tomato Plant Growth and Drought Stress Tolerance

  2021cites this paper
• Transcriptome and metabolome analysis of stress tolerance to aluminium in Vitis quinquangularis

  2021cites this paper
• Genetic analysis of potato tuber metabolite composition: Genome‐wide association studies applied to a nontargeted metabolome

  2020cites this paper
• Comprehensive identification of minor components and bioassay-guided isolation of an unusual antioxidant from Azolla imbricata using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry combined with multicomponent knockout and bioactivity evaluation.

  2020cites this paper
• Mass Spectrometric Approaches to Study the Metabolism of Jasmonates: Biotransformation of Exogenously Supplemented Methyl Jasmonate by Cell Suspension Cultures of Moringa oleifera.

  2020cites this paper
• Quantitative Jasmonate Profiling Using a High-Throughput UPLC-NanoESI-MS/MS Method.

  2020cites this paper
• Foliar Application of Chitosan Increases Tomato Growth and Influences Mycorrhization and Expression of Endochitinase-Encoding Genes

  2020influential citation
• A High-throughput Method for Fingerprinting of Rhizobial Free Fatty Acids by the Combination of a Thin Film Chemical Deposition Technique and Matrix-assisted Laser Desorption/ionization Time-of Flight Mass Spectrometry

  2020cites this paper
• Does Protein Glycation Impact on the Drought-Related Changes in Metabolism and Nutritional Properties of Mature Pea (Pisum sativum L.) Seeds?

  2020cites this paper
• PbGA20ox2 Regulates Fruit Set and Induces Parthenocarpy by Enhancing GA4 Content

  2020cites this paper
• Systemin-mediated long-distance systemic defense responses.

  2020cites this paper
• Detection of seven phytohormones in peanut tissues by ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectrometry

  2020cites this paper
• Jasmonate is required for the response to osmotic stress in rice

  2020cites this paper