Topography of glycosylation reactions in the rough endoplasmic reticulum membrane.

The translocation of UDP-glucose and GDP-mannose from an external to a luminal compartment has been examined in rat liver vesicles derived from the rough endoplasmic reticulum (RER). RER vesicles with the same topographical orientation as in vivo were incubated with a mixture of [3H]UDP-glucose and UDP-[14C]glucose to demonstrate that the intact sugar nucleotide was translocated into the lumen of the vesicles. The translocation of UDP-glucose was dependent on temperature and was saturable at high concentrations of the sugar nucleotide. The transfer of glucose to endogenous acceptors was dependent on the translocation of UDP-glucose into the lumen of the RER since leaky vesicles resulted in both a decrease in transport and transfer of glucose to endogenous acceptors. Preliminary results suggest that the mechanism of UDP-glucose transport into RER-derived vesicles is via a coupled exchange with luminal UMP. Using the same experimental approach to detect translocation of UDP-glucose into the lumen of RER vesicles, we were unable to detect transport of GDP-mannose. Incubation of leaky vesicles with GDP-mannose resulted in stimulation of the amount of mannose transferred to endogenous acceptors, in marked contrast to that observed for UDP-glucose and UDP-N-acetylglucosamine. These results suggest that whereas UDP-glucose is translocated across the RER membrane in vitro, GDP-mannose is not transported. In addition, these results tentatively suggest that there is asymmetric synthesis of the lipid-linked oligosaccharides within the membrane of the RER.

• Publication year

  1986

• Venue

  Journal of Biological Chemistry

• Publication date

  1986-05-25

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(19)62689-xPMID 2422171
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Mechanism of galactosylation in the Golgi apparatus. A Chinese hamster ovary cell mutant deficient in translocation of UDP-galactose across Golgi vesicle membranes.

  1986cited by this paper
• The intracellular accumulation of UDP-N-acetylhexosamines is concomitant with the inability of human colon cancer cells to differentiate.

  1985cited by this paper
• UDPglucose-ceramide glucosyltransferase from porcine submaxillary glands is associated with the Golgi apparatus.

  1985cited by this paper
• Translocation of UDP-N-acetylglucosamine into vesicles derived from rat liver rough endoplasmic reticulum and Golgi apparatus.

  1985cited by this paper
• Topology of UDP-galactose cleavage in relation to N-acetyl-lactosamine formation in Golgi vesicles. Translocation of activated galactose.

  1985cited by this paper
• Mechanisms of glycosylation and sulfation in the Golgi apparatus: evidence for nucleotide sugar/nucleoside monophosphate and nucleotide sulfate/nucleoside monophosphate antiports in the Golgi apparatus membrane.

  1984cited by this paper
• Translocation across Golgi vesicle membranes: a CHO glycosylation mutant deficient in CMP-sialic acid transport.

  1984cited by this paper
• Transmembrane movement of oligosaccharide-lipids during glycoprotein synthesis.

  1984cited by this paper
• Evidence for coupling between transport of UDP-glucose and its synthesis by membrane-bound pyrophosphorylase in Golgi apparatus of cat liver.

  1984cited by this paper
• Yeast mutants deficient in protein glycosylation.

  1983cited by this paper
• Mechanism of glycosylation in the Golgi apparatus.

  1983cited by this paper
• Tunicamycin inhibits ganglioside biosynthesis in rat liver Golgi apparatus by blocking sugar nucleotide transport across the membrane vesicles.

  1983cited by this paper
• Transport of sugar nucleotides into rat liver Golgi. A new Golgi marker activity.

  1982cited by this paper
• Transmembrane organization of protein glycosylation. Mature oligosaccharide-lipid is located on the luminal side of microsomes from Chinese hamster ovary cells.

  1982cited by this paper
• A convenient enzymic synthesis of guanosine diphosphate-[14C]mannose.

  1982cited by this paper
• Transmembrane assembly of N-linked glycoproteins. Studies on the topology of saccharide synthesis.

  1982cited by this paper
• Mutant of Chinese hamster ovary cells with altered mannose 6-phosphate receptor activity is unable to synthesize mannosylphosphoryldolichol.

  1982cited by this paper
• Dolichyl phosphate-mediated mannosyl transfer through liposomal membranes.

  1982cited by this paper
• Uridylate trapping induced by the C-2-modified D-glucose analogs glucosone, fluoroglucose, and glucosamine.

  1982cited by this paper
• Topography of sialoglycoproteins and sialyltransferases in mouse and rat liver Golgi.

  1981cited by this paper
• Glucose starvation alters lipid-linked oligosaccharide biosynthesis in Chinese hamster ovary cells.

  1981cited by this paper
• Synthesis and processing of asparagine-linked oligosaccharides.

  1981cited by this paper
• Effect of energy depletion on the glycosylation of a viral glycoprotein.

  1981cited by this paper
• N-Linked glycoprotein assembly. Evidence that oligosaccharide attachment occurs within the lumen of the endoplasmic reticulum.

  1980cited by this paper
• Transmembrane location of oligosaccharide-lipid synthesis in microsomal vesicles.

  1980cited by this paper
• Kinetics of glycosylation and intracellular transport of sialoglycoproteins in mouse liver.

  1980cited by this paper
• CMP-N-acetylneuraminic acid: isolation from and penetration into mouse liver microsomes.

  1980cited by this paper
• Structure of the lipid-linked oligosaccharides that accumulate in class E Thy-1-negative mutant lymphomas.

  1979cited by this paper
• Temporal relationship of translation and glycosylation of immunoglobulin heavy and light chains.

  1978cited by this paper
• Coupled cell-free synthesis, segregation, and core glycosylation of a secretory protein.

  1978cited by this paper
• Isolation of wheat germ agglutinin-resistant clones of Chinese hamster ovary cells deficient in membrane sialic acid and galactose.

  1977cited by this paper
• A simplification of the protein assay method of Lowry et al. which is more generally applicable.

  1977cited by this paper
• Membrane assembly in vitro: synthesis, glycosylation, and asymmetric insertion of a transmembrane protein.

  1977cited by this paper
• Measurement of glucose 6-phosphate penetration into liver microsomes. Confirmation of substrate transport in the glucose-6-phosphatase system.

  1977cited by this paper
• Glucosyltransferase activity in calf pancreas microsomes. Formation of dolichyl D[14C]glucosyl phosphate and 14C-labeled lipid-linked oligosaccharides from UDP-D-[14C]glucose.

  1977cited by this paper
• Evidence for specific transport of uridine diphosphate galactose across the Golgi membrane of rat mammary gland.

  1976cited by this paper
• Studies on fetuin, a glycoprotein of fetal serum. I. Isolation, chemical composition, and physiochemical properties.

  1960cited by this paper

• Delivery of Nucleotide Sugars to the Mammalian Golgi: A Very Well (un)Explained Story

  2022cites this paper
• Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose

  2020cites this paper
• Structural basis for the delivery of activated sialic acid into Golgi for sialyation

  2019cites this paper
• Supplementation of specific carbohydrates results in enhanced deposition of chondrogenic‐specific matrix during mesenchymal stem cell differentiation

  2018cites this paper
• Cytological aspects of cell wall synthesis

  2016cites this paper
• Roles of the nucleotide sugar transporters (SLC35 family) in health and disease.

  2013cites this paper
• Molecular mechanisms of purine and pyrimidine nucleotide release.

  2011cites this paper
• Nucleotide release by airway epithelia.

  2011cites this paper
• A Structure-Function Investigation of Cytidine 5'-Monophosphate N-Acetylneuraminic Acid Transport Protein

  2011cites this paper
• Endoplasmic Reticulum/Golgi Nucleotide Sugar Transporters Contribute to the Cellular Release of UDP-sugar Signaling Molecules*

  2009cites this paper
• Mechanisms of transmitter release from astrocytes

  2009cites this paper
• A thermostable dolichol phosphoryl mannose synthase responsible for glycoconjugate synthesis of the hyperthermophilic archaeon Pyrococcus horikoshii

  2008cites this paper
• Use of Giardia, which appears to have a single nucleotide-sugar transporter for UDP-GlcNAc, to identify the UDP-Glc transporter of Entamoeba.

  2008cites this paper
• Transport and transporters in the endoplasmic reticulum.

  2007cites this paper
• Nucleotide-sugar transporters: structure, function and roles in vivo.

  2006cites this paper
• Alg14 Recruits Alg13 to the Cytoplasmic Face of the Endoplasmic Reticulum to Form a Novel Bipartite UDP-N-acetylglucosamine Transferase Required for the Second Step of N-Linked Glycosylation*

  2005cites this paper
• ire-1-dependent Transcriptional Up-regulation of a Lumenal Uridine Diphosphatase from Caenorhabditis elegans*

  2004cites this paper
• mannosyltransferases of the endoplasmic reticulum

  2004cites this paper
• Roles of N-linked glycans in the endoplasmic reticulum.

  2004cites this paper
• Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum.

  2004cites this paper
• Nucleoside Diphosphatase and Glycosyltransferase Activities Can Localize to Different Subcellular Compartments in Schizosaccharomyces pombe*

  2003cites this paper
• The contribution of N-glycans and their processing in the endoplasmic reticulum to glycoprotein biosynthesis.

  2003cites this paper
• Release of cellular UDP-glucose as a potential extracellular signaling molecule.

  2003cites this paper
• Translocation of lipid-linked oligosaccharides across the ER membrane requires Rft 1 protein

  2002cites this paper
• Translocation of lipid-linked oligosaccharides across the ER membrane requires Rft1 protein

  2002cites this paper
• Transmembrane movement of dolichol linked carbohydrates during N-glycoprotein biosynthesis in the endoplasmic reticulum.

  2002cites this paper
• N-glycan processing and glycoprotein folding.

  2001cites this paper
• The UDPase activity of the Kluyveromyces lactis Golgi GDPase has a role in uridine nucleotide sugar transport into Golgi vesicles.

  2001cites this paper
• First Apyrase Splice Variants Have Different Enzymatic Properties*

  2000cites this paper
• Sequencing, functional expression and characterization of rat NTPDase6, a nucleoside diphosphatase and novel member of the ecto-nucleoside triphosphate diphosphohydrolase family.

  2000cites this paper
• The Leishmania GDP-mannose transporter is an autonomous, multi-specific, hexameric complex of LPG2 subunits.

  2000cites this paper
• Tyrosinase and glycoprotein folding: roles of chaperones that recognize glycans.

  2000cites this paper
• Protein glucosylation and its role in protein folding.

  2000cites this paper
• Uridine diphosphate-glucose transport into the endoplasmic reticulum of Saccharomyces cerevisiae: in vivo and in vitro evidence.

  1999influential citation
• Glycoprotein reglucosylation and nucleotide sugar utilization in the secretory pathway: identification of a nucleoside diphosphatase in the endoplasmic reticulum

  1999influential citation
• Reglucosylation of glycoproteins and quality control of glycoprotein folding in the endoplasmic reticulum of yeast cells.

  1999cites this paper
• Core Issues: Building The Groundwork for N-Linked Sugars

  1998cites this paper
• Nucleotide Sugars, Nucleotide Sulfate, and ATP Transporters of the Endoplasmic Reticulum and Golgi Apparatus a,

  1998cites this paper
• Identification of endoplasmic reticulum proteins involved in glycan assembly: synthesis and characterization of P3-(4-azidoanilido)uridine 5'-triphosphate, a membrane-topological photoaffinity probe for uridine diphosphate-sugar binding proteins.

  1998cites this paper
• The quality control of glycoprotein folding in the endoplasmic reticulum, a trip from trypanosomes to mammals.

  1998cites this paper
• Sugars And Proteins: Why They Get It Together

  1998cites this paper
• Nucleotide sugar transporters: elucidation of their molecular identity and its implication for future studies.

  1998cites this paper
• Transport of nucleotide sugars, nucleotide sulfate and ATP into the lumen of the Golgi apparatus

  1997cites this paper
• Promotion of transferrin folding by cyclic interactions with calnexin and calreticulin

  1997cites this paper
• The Golgi Apparatus

  1997cites this paper
• Characterization of UDP-glucuronic acid transport in rat liver microsomal vesicles with photoaffinity analogs.

  1994cites this paper
• How N-linked oligosaccharides affect glycoprotein folding in the endoplasmic reticulum.

  1994cites this paper
• The Golgi guanosine diphosphatase is required for transport of GDP-mannose into the lumen of Saccharomyces cerevisiae Golgi vesicles.

  1994cites this paper
• A recent approach to the study of dolichyl monophosphate topology in the rough endoplasmic reticulum.

  1994cites this paper
• Purification to homogeneity of UDP-glucose:glycoprotein glucosyltransferase from Schizosaccharomyces pombe and apparent absence of the enzyme fro Saccharomyces cerevisiae.

  1994cites this paper
• Metabolites of 2-Deoxy-[14C]Glucose in Plasma and Brain: Influence on Rate of Glucose Utilization Determined with Deoxyglucose Method in Rat Brain

  1993cites this paper
• N-glycosylation in trypanosomatid protozoa.

  1993cites this paper
• A novel mono-branched lipid phosphate acts as a substrate for dolichyl phosphate mannose synthetase.

  1993cites this paper
• Topography of glycosylation and UDP-xylose production.

  1993cites this paper
• The glycolipid anchor of membrane surface proteins.

  1993cites this paper
• Topology of nucleotide-sugar:dolichyl phosphate glycosyltransferases involved in the dolichol pathway for protein glycosylation in native rat liver microsomes.

  1993cites this paper
• Transport of Nucleotide Derivatives into Endoplasmic Reticulum and Golgiapparatus Derived Vesicles: a Dissertation

  1993cites this paper
• Topography of glycosylation reactions in the endoplasmic reticulum.

  1992cites this paper
• Purification to apparent homogeneity and partial characterization of rat liver UDP-glucose:glycoprotein glucosyltransferase.

  1992cites this paper
• Protein glycosylation in yeast

  1992cites this paper
• Application of 5-azido-UDP-glucose and 5-azido-UDP-glucuronic acid photoaffinity probes for the determination of the active site orientation of microsomal UDP-glucosyltransferases and UDP-glucuronosyltransferases.

  1992cites this paper
• Reconstitution into proteoliposomes and partial purification of the Golgi apparatus membrane UDP-galactose, UDP-xylose, and UDP-glucuronic acid transport activities.

  1992cites this paper
• Failure of expression of the phenobarbital-induced enhancement of UDP-glycosyltransferases in native, sealed endoplasmic reticulum vesicles from rat liver.

  1992cites this paper
• An anion-exchange radioassay for glucose 6-phosphate phosphatase: use in topological studies with endoplasmic reticulum vesicles.

  1992cites this paper
• Translocation of ATP into the lumen of rough endoplasmic reticulum-derived vesicles and its binding to luminal proteins including BiP (GRP 78) and GRP 94.

  1992cites this paper
• Genetic regulation of asparagine-linked oligosaccharide synthesis.

  1991cites this paper
• Translocation of the yeast dolichol-phosphate-mannose synthase into microsomal membranes.

  1991cites this paper
• Determination of the intracellular sites and topology of glucosylceramide synthesis in rat liver.

  1991cites this paper
• Two different mutants blocked in synthesis of dolichol-phosphoryl-mannose do not add glycophospholipid anchors to membrane proteins: quantitative correction of the phenotype of a CHO cell mutant with tunicamycin

  1991cites this paper
• Lipid-linked Saccharides in Plants: Intermediates in the Synthesis of N-linked Glycoproteins

  1990cites this paper
• Complementing mutant alleles define three loci involved in mannosylation of Man5-GlcNAc2-P-P-dolichol in Chinese hamster ovary cells

  1990cites this paper
• Evidence for the involvement of a 35-kDa membrane protein in the synthesis of glucosylphosphoryldolichol

  1990cites this paper
• Topography of initiation of N-glycosylation reactions.

  1990cites this paper
• A block at Man5GlcNAc2-pyrophosphoryldolichol in intact but not disrupted castanospermine and swainsonine-resistant Chinese hamster ovary cells.

  1990cites this paper
• Purification and characterization of dolichyl-P-mannose:Man5(GlcNAc)2-PP-dolichol mannosyltransferase.

  1990cites this paper
• Intracellular and extracellular flow of dolichol.

  1990cites this paper
• Properties of membrane-bound bilirubin UDP-glucuronyltransferase in rough and smooth endoplasmic reticulum and in the nuclear envelope from rat liver.

  1989cites this paper
• Topological orientation of mitochondrial GDPmannose: dolichyl-monophosphate mannosyltransferase in the outer membrane.

  1989cites this paper
• Topography of glycosylation in yeast: characterization of GDPmannose transport and lumenal guanosine diphosphatase activities in Golgi-like vesicles.

  1989cites this paper
• Reconstitution of Golgi vesicle CMP-sialic acid and adenosine 3'-phosphate 5'-phosphosulfate transport into proteoliposomes.

  1989cites this paper
• Membrane biology in yeast as probed with enveloped viruses.

  1989cites this paper
• Glucosylation of glycoproteins by mammalian, plant, fungal, and trypanosomatid protozoa microsomal membranes.

  1989cites this paper
• Purification of GDP mannose:dolichyl-phosphate O-beta-D-mannosyltransferase from Saccharomyces cerevisiae.

  1989cites this paper
• Organization of mitochondrial UDP-glucose: Dolichylmonophosphate glucosyltransferase in the outer membrane

  1989cites this paper
• Bile pigment metabolism.

  1989cites this paper
• Fungal cell wall synthesis and assembly.

  1989cites this paper
• Virally Infected Cells

  1989cites this paper
• Comparative studies on mannosylphosphoryl dolichol and glucosylphosphoryl dolichol synthases.

  1989cites this paper
• Carrier-mediated translocation of uridine diphosphate glucose into the lumen of endoplasmic reticulum-derived vesicles from rat liver.

  1988cites this paper
• Intracellular transport in interphase and mitotic yeast cells.

  1988cites this paper
• Amplification and molecular cloning of the hamster tunicamycin-sensitive N-acetylglucosamine-1-phosphate transferase gene. The hamster and yeast enzymes share a common peptide sequence.

  1988cites this paper
• A membrane transporter mediates access of uridine 5'-diphosphoglucuronic acid from the cytosol into the endoplasmic reticulum of rat hepatocytes: implications for glucuronidation reactions.

  1988cites this paper
• The Assembly and Transfer of Oligosaccharide Chains to Proteins

  1988cites this paper
• Protein glycosylation in yeast.

  1987cites this paper
• Topography of glycosylation in the rough endoplasmic reticulum and Golgi apparatus.

  1987cites this paper
• Endogenous esterification of bilirubin by liver microsomes. Evidence for an intramicrosomal pool of UDP-glucose and lumenal orientation of bilirubin UDP-glycosyltransferase.

  1987cites this paper
• Subcellular organization of glycosylation in mammalian cells.

  1987cites this paper
• Protein glycosylation in the endoplasmic reticulum: current topological issues.

  1987cites this paper
• Subcellular site of synthesis of the N-acetylgalactosamine (alpha 1-0) serine (or threonine) linkage in rat liver.

  1987cites this paper
• A monoclonal antibody against a family of nuclear pore proteins (nucleoporins): O-linked N-acetylglucosamine is part of the immunodeterminant.

  1987cites this paper