SCIENCE@home

Human whole-genome shotgun sequencing.

J. Weber,E. Myers

Published 1997 in Genome Research

ABSTRACT

Large-scale sequencing of the human genome is now under way (Boguski et al. 1996; Marshall and Pennisi 1996). Although at the beginning of the Genome Project, many doubted the scientific value of sequencing the entire human genome, these doubts have evaporated almost entirely (Gibbs 1995; Olson 1995). Primary reasons for generating the human genomic sequence are listed in Table 1. The approach being taken for human genomic sequencing is the same as that used for the Saccharomyces cerevisiae and Caenorhabditis elegans genomes, namely construction of overlapping arrays of large insert Escherichia coli clones, followed by complete sequencing of these clones one at a time. In this article, we outline an alternative approach to sequencing the human and other large genomes, which we argue is less costly and more informative than the clone-by-clone approach.

PUBLICATION RECORD

CITATION MAP

EXTRACTION MAP

CLAIMS

  • No claims are published for this paper.

CONCEPTS

  • No concepts are published for this paper.

REFERENCES

Showing 1-71 of 71 references · Page 1 of 1

CITED BY

Showing 1-100 of 348 citing papers · Page 1 of 4

1234
Next