Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces

No abstract is available for this paper.

• Publication year

  2004

• Venue

  Applied and Environmental Microbiology

• Publication date

  2004-12-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1128/AEM.70.12.7220-7228.2004PMID 15574920PMCID PMC535136
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Quantitative (real-time) PCR

  2005cited by this paper
• Quantitative PCR with 16S rRNA-Gene-Targeted Species-Specific Primers for Analysis of Human Intestinal Bifidobacteria

  2004cited by this paper
• Development of multi-color FISH method for analysis of seven Bifidobacterium species in human feces.

  2004cited by this paper
• Comparison of real-time PCR with SYBR Green I or 5'-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria.

  2003cited by this paper
• Phylogenetic Analysis of the Human Gut Microbiota Using 16S rDNA Clone Libraries and Strictly Anaerobic Culture‐Based Methods

  2002cited by this paper
• Development of 16S rRNA-Gene-Targeted Group-Specific Primers for the Detection and Identification of Predominant Bacteria in Human Feces

  2002cited by this paper
• Extensive Set of 16S rRNA-Based Probes for Detection of Bacteria in Human Feces

  2002influential reference
• Fecal Microbial Diversity in a Strict Vegetarian as Determined by Molecular Analysis and Cultivation

  2002cited by this paper
• Diet-Dependent Shifts in the Bacterial Population of the Rumen Revealed with Real-Time PCR

  2001cited by this paper
• Rapid Detection and Quantification of Five Periodontopathic Bacteria by Real‐Time PCR

  2001cited by this paper
• Quantitative real-time PCR for Porphyromonas gingivalis and total bacteria.

  2000cited by this paper
• Quantification of Bacterial Groups within Human Fecal Flora by Oligonucleotide Probe Hybridization

  2000cited by this paper
• Rapid identification of 11 human intestinal Lactobacillus species by multiplex PCR assays using group- and species-specific primers derived from the 16S-23S rRNA intergenic spacer region and its flanking 23S rRNA.

  2000cited by this paper
• Development of 16S rRNA-Based Probes for theCoriobacterium Group and the Atopobium Cluster and Their Application for Enumeration of Coriobacteriaceaein Human Feces from Volunteers of Different Age Groups

  2000cited by this paper
• Detection and Identification of GastrointestinalLactobacillus Species by Using Denaturing Gradient Gel Electrophoresis and Species-Specific PCR Primers

  2000cited by this paper
• Comparison of the Oral Bacterial Flora in Saliva from a Healthy Subject and Two Periodontitis Patients by Sequence Analysis of 16S rDNA Libraries

  2000cited by this paper
• Rapid identification and quantification of Collinsella aerofaciens using PCR.

  2000cited by this paper
• Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers

  1999cited by this paper
• A new version of the RDP (Ribosomal Database Project)

  1999cited by this paper
• Direct Analysis of Genes Encoding 16S rRNA from Complex Communities Reveals Many Novel Molecular Species within the Human Gut

  1999influential reference
• Variations of Bacterial Populations in Human Feces Measured by Fluorescent In Situ Hybridization with Group-Specific 16S rRNA-Targeted Oligonucleotide Probes

  1998cited by this paper
• Temperature Gradient Gel Electrophoresis Analysis of 16S rRNA from Human Fecal Samples Reveals Stable and Host-Specific Communities of Active Bacteria

  1998cited by this paper
• Identification and quantification of Bifidobacterium species isolated from food with genus-specific 16S rRNA-targeted probes by colony hybridization and PCR

  1997cited by this paper
• Product differentiation by analysis of DNA melting curves during the polymerase chain reaction.

  1997cited by this paper
• The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.

  1997cited by this paper
• PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples

  1996cited by this paper
• Normal microflora: An introduction to microbes inhabiting the human body

  1995cited by this paper
• Intestinal floras of populations that have a high risk of colon cancer

  1995cited by this paper
• Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples

  1995cited by this paper
• Phylogeny of Bacteroides, Prevotella, and Porphyromonas spp. and related bacteria

  1994cited by this paper
• The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations.

  1994cited by this paper
• Comparison of fecal microflora of elderly persons in rural and urban areas of Japan

  1989cited by this paper
• Probiotics in man and animals.

  1989cited by this paper
• Bacterial evolution

  1987cited by this paper
• CHAPTER 1 – Normal Indigenous Intestinal Flora

  1983cited by this paper
• Human fecal flora: the normal flora of 20 Japanese-Hawaiians.

  1974cited by this paper
• Effect of diet on human fecal flora: comparison of Japanese and American diets.

  1974cited by this paper

• Engineering lactococci to reduce human lactose intolerance; biotechnology and risk/benefit assessment toward the gut microbiota of lactose-intolerants.

  2026cites this paper
• Early Establishment of Bifidobacterium-Dominant Microbiome in Planned Cesarean-Born Infants via Bifidobacterium breve strain Yakult Supplementation: A Randomized, Double-blind, Placebo-controlled, Parallel-group Pilot Study

  2026cites this paper
• A probiotic Brevibacillus laterosporus promotes chicken growth performance immunity and gut health

  2025cites this paper
• Evaluation of the incremental effect of fasting length on pH, select cecal microbial groups, cecal volatile fatty acid concentrations, and secretory IgA excretion in roosters

  2025cites this paper
• Preventive and therapeutic effects of co-administration of Bacteroides thetaiotaomicron and infliximab on dextran sodium sulfate-induced colitis in mice.

  2025cites this paper
• Characteristics of the gut microbiota in patients with advanced non-small cell lung cancer who responded to immune checkpoint inhibitors

  2025cites this paper
• Immunosuppressant-Induced Alteration of Gut Microbiota Causes Loss of Skeletal Muscle Mass: Evidence from Animal Experiments Using Mice and Observational Study on Humans

  2025cites this paper
• Lactobacillus acidophilus TW01 Mitigates PM2.5-Induced Lung Injury and Improves Gut Health in Mice

  2025cites this paper
• A randomized controlled study protocol of the TOBBI trial: the effect of a 6 weeks intervention with synbiotics on the recovery speed of the gut microbiota after antibiotic treatment in Dutch toddlers

  2025cites this paper
• Prebiotic Effect of Polysaccharides and Flavonoids from Passiflora foetida Fruits on the Human Intestinal Microbiota Associated with Obesity

  2025cites this paper
• Selection, Comparative Genomics, and Potential Probiotic Features of Escherichia coli 5C, a pks-Negative Strain Isolated from Healthy Infant Donor Feces.

  2025cites this paper
• Comparative Study of Prebiotics for Infants Using a Fecal Culture System: Insights into Responders and Non-Responders

  2024cites this paper
• Microbiota analysis in individuals with type two diabetes mellitus and end‑stage renal disease: A pilot study

  2024cites this paper
• The gut-brain-axis: A positive relationship between gut microbial dysbiosis and glioblastoma brain tumour

  2024cites this paper
• Inappropriate diet and hygiene status affect the progression of diabetic kidney disease by causing dysbiosis.

  2024cites this paper
• Assessment of salivary microbiota profile as a potential diagnostic tool for pediatric celiac disease

  2024cites this paper
• The beneficial impact of ketogenic diets on chemically-induced colitis in mice depends on the diet's lipid composition.

  2024cites this paper
• Unveiling the influence of a probiotic combination of Heyndrickxia coagulans and Lacticaseibacillus casei on healthy human gut microbiota using the TripleSHIME® system.

  2024cites this paper
• YOGURT ACID WHEY POWDER DIETARY INCLUSION LEVEL MODULATES BROILER CECAL MICROBIOTA COMPOSITION AND METABOLIC ACTIVITY

  2024cites this paper
• Sucralose Influences the Productive Performance, Carcass Traits, Blood Components, and Gut Microflora Using 16S rRNA Sequencing of Growing APRI-Line Rabbits

  2024cites this paper
• Additive effect of mixtures of multiple strains combining the effects of single strains on human gut microbiota in vitro

  2024cites this paper
• M-Batches to Simulate Luminal and Mucosal Human Gut Microbial Ecosystems: A Case Study of the Effects of Coffee and Green Tea

  2024cites this paper
• Reduction of butyrate-producing bacteria in the gut microbiome of Japanese patients with pancreatic cancer.

  2024cites this paper
• Antibiotic-induced gut microbe dysbiosis alters neurobehavior in mice through modulation of BDNF and gut integrity.

  2024cites this paper
• Prebiotic effects of commercial apple juice in high-fat diet fed rat

  2024cites this paper
• Gut microbiome signatures associated with type 2 diabetes in obesity in Mongolia

  2024cites this paper
• Analysis of microbial colonies from the first-pass meconium of healthy neonates: Comparison of vaginally and cesarean section born

  2023cites this paper
• Serum Levels of IFABP2 and Differences in Lactobacillus and Porphyromonas gingivalis Abundance on Gut Microbiota Are Associated with Poor Therapeutic Response in Rheumatoid Arthritis: A Pilot Study

  2023cites this paper
• Salmonella enterica induces biogeography-specific changes in the gut microbiome of pigs

  2023cites this paper
• The Effects of Black Tea Consumption on Intestinal Microflora-A Randomized Single-Blind Parallel-Group, Placebo-Controlled Study.

  2023cites this paper
• Effects of Intramammary Antimicrobial Treatment on the Milk Microbiota Composition in Mild Clinical Bovine Mastitis Caused by Gram-Positive Bacteria

  2023influential citation
• Effect of a probiotic fermented milk supplementation on behavior and sleep

  2023cites this paper
• Butyrogenic, bifidogenic and slight anti-inflammatory effects of a green kiwifruit powder (Kiwi FFG®) in a human gastrointestinal model simulating mild constipation.

  2023cites this paper
• In Vitro Fermentation of Different Indigestible Glucans with Varying Physico-Chemical Properties by Human Fecal Microbiota

  2023cites this paper
• Guar gum as galactomannan source induces dysbiosis and reduces performance in broiler chickens and dietary β-mannanase restores the gut homeostasis

  2023cites this paper
• Restrictive diets have a beneficial impact on DSS-induced colitis in male mice.

  2023cites this paper
• Gut Microbiome and Microbiome-Derived Metabolites in Patients with End-Stage Kidney Disease

  2023cites this paper
• Characterization of gut dominant microbiota in obese patients with nonalcoholic fatty liver disease

  2023cites this paper
• Evaluation of a Natural Phytogenic Formulation as an Alternative to Pharmaceutical Zinc Oxide in the Diet of Weaned Piglets

  2023cites this paper
• Intestinal microbiota changes pre- and post-fecal microbiota transplantation for treatment of recurrent Clostridioides difficile infection among Iranian patients with concurrent inflammatory bowel disease

  2023cites this paper
• Quantitative analysis of the three gut microbiota in UC and non-UC patients using real-time PCR.

  2023cites this paper
• Association of gut microbiota with portal vein pressure in patients with liver cirrhosis undergoing living donor liver transplantation

  2023cites this paper
• Dietary and phytogenic inclusion effects on the broiler chicken cecal ecosystem

  2023cites this paper
• Effect of the gut microbiota on the expression of genes that are important for maintaining skin function: Analysis using aged mice

  2023cites this paper
• Prebiotic potential of new sweeteners based on the simultaneous biosynthesis of galactooligosaccharides and enzymatically modified steviol glycosides.

  2023cites this paper
• Investigation of supplementation with a combination of fermented bean dregs and wheat bran for improving the growth performance of the sow

  2023cites this paper
• Faecal Microbiota Characterisation of Potamochoerus porcus Living in a Controlled Environment

  2023cites this paper
• Human Adult Microbiota in a Static Colon Model: AhR Transcriptional Activity at the Crossroads of Host–Microbe Interaction

  2022cites this paper
• Detecting bacterial infections in wounds: a review of biosensors and wearable sensors in comparison with conventional laboratory methods.

  2022cites this paper
• Impact of Intestinal Microbiota on Cognitive Flexibility by a Novel Touch Screen Operant System Task in Mice

  2022cites this paper
• Gut-Thyroid axis: How gut microbial dysbiosis associated with euthyroid thyroid cancer

  2022cites this paper
• Effects of dietary protein level on small intestinal morphology, occludin protein, and bacterial diversity in weaned piglets

  2022cites this paper
• Microbiome, Mycobiome and Related Metabolites Alterations in Patients with Metabolic Syndrome—A Pilot Study

  2022cites this paper
• Spatial distribution of live gut microbiota and bile acid metabolism in various parts of human large intestine

  2022cites this paper
• Fiber Consumption Mediates Differences in Several Gut Microbes in a Subpopulation of Young Mexican Adults

  2022cites this paper
• Effects of

  2022cites this paper
• Gut- and oral-dysbiosis differentially impact spinal- and bulbar-onset ALS, predicting ALS severity and potentially determining the location of disease onset

  2022cites this paper
• Prebiotic Potential of a New Sweetener Based on Galactooligosaccharides and Modified Mogrosides

  2022cites this paper
• Polyphenols

  2022cites this paper
• Effects of Catalase on Growth Performance, Antioxidant Capacity, Intestinal Morphology, and Microbial Composition in Yellow Broilers

  2022cites this paper
• Evaluation of the Effect of Different Dietary Lipid Sources on Dogs’ Faecal Microbial Population and Activities

  2022cites this paper
• Gut Microbiota Modulation, Anti-Diabetic and Anti-Inflammatory Properties of Polyphenol Extract from Mung Bean Seed Coat (Vigna radiata L.)

  2022cites this paper
• Microbiota analysis in the hemodialysis population - Focusing on Enterobacteriaceae.

  2022cites this paper
• Expression of α- and ε-toxin genes in Clostridium perfringens type D vaccine strain in contact with the Caco-2 cell line

  2022cites this paper
• THE EFFECTS OF COPRA-MANNO-OLIGOSACCHARIDE ON BACTERIUM AGGREGATION ACTIVITY AND MICROBIOLOGICAL CHANGES IN A SIMULATED DIGESTIVE TRACT

  2022cites this paper
• Fish oil and probiotics supplementation through milk chocolate improves spatial learning and memory in male Wistar rats

  2022cites this paper
• Alterations in the gut microbiome in patients with esophageal carcinoma in response to esophagectomy and neoadjuvant treatment

  2022cites this paper
• Impacts of Menstruation, Community Type, and an Oral Yeast Probiotic on the Vaginal Microbiome

  2022cites this paper
• Ionizing Radiation from Radiopharmaceuticals and the Human Gut Microbiota: An Ex Vivo Approach

  2022influential citation
• Effect of Radium-223 on the Gut Microbiota of Prostate Cancer Patients: A Pilot Case Series Study

  2022influential citation
• Traumatic brain injury alters the gut-derived serotonergic system and associated peripheral organs.

  2022cites this paper
• Assessment of the Effects of Edible Microalgae in a Canine Gut Model

  2022cites this paper
• Supplementing oat hulls to the diet of suckling piglets altered their intestinal tract and colonic microbiota development.

  2022cites this paper
• Cooperative action of gut-microbiota-accessible carbohydrates improves host metabolic function.

  2022cites this paper
• A prospective interventional trial on the effect of periodontal treatment on Fusobacterium nucleatum abundance in patients with colorectal tumours

  2021cites this paper
• Potential Association Between Dietary Fibre and Humoral Response to the Seasonal Influenza Vaccine

  2021cites this paper
• Cashew nut shell liquid potentially mitigates methane emission from the feces of Thai native ruminant livestock by modifying fecal microbiota.

  2021cites this paper
• Administration of a Probiotic Mixture Ameliorates Cisplatin-Induced Mucositis and Pica by Regulating 5-HT in Rats

  2021cites this paper
• Effect of the standard herbal preparation, STW5, treatment on dysbiosis induced by dextran sodium sulfate in experimental colitis

  2021cites this paper
• Yearly changes in the composition of gut microbiota in the elderly, and the effect of lactobacilli intake on these changes

  2021cites this paper
• Lactobacillus paracasei L9 improves colitis by expanding butyrate-producing bacteria that inhibit the IL-6/STAT3 signaling pathway.

  2021cites this paper
• Maternal Probiotic or Synbiotic Supplementation Modulates Jejunal and Colonic Antioxidant Capacity, Mitochondrial Function, and Microbial Abundance in Bama Mini-piglets

  2021cites this paper
• Alterations in children’s sub-dominant gut microbiota by HIV infection and anti-retroviral therapy

  2021cites this paper
• Dissecting Individual Interactions between Pathogenic and Commensal Bacteria within a Multispecies Gut Microbial Community

  2021cites this paper
• Gut Microbiome of Indonesian Adults Associated with Obesity and Type 2 Diabetes: A Cross-Sectional Study in an Asian City, Yogyakarta

  2021cites this paper
• Effects of Synbiotic Supplementation on Chronic Inflammation and the Gut Microbiota in Obese Patients with Type 2 Diabetes Mellitus: A Randomized Controlled Study

  2021cites this paper
• Carob fruit extract-enriched meat, as preventive and curative treatments, improves gut microbiota and colonic barrier integrity in a late-stage T2DM model.

  2021cites this paper
• A Bacterial Bile Acid Metabolite Modulates Treg Activity through the Nuclear Hormone Receptor NR4A1

  2021cites this paper
• Modulation and metabolism of obesity-associated microbiota in a dynamic simulator of the human gut microbiota

  2021cites this paper
• Modulation of the Gut Microbiota and Serum Biomarkers After Laparoscopic Sleeve Gastrectomy: a 1-Year Follow-Up Study

  2021cites this paper
• Alterations in the Composition of the Gut Microbiota in Celiac Disease, Non-Coeliac Gluten Sensitivity and Irritable Bowel Syndrome

  2021cites this paper
• Are Fecal Metabolome and Microbiota Profiles Correlated with Autism Severity? A Cross-Sectional Study on ASD Preschoolers

  2021cites this paper
• The e cacy of Bi dobacterium bi dum G9-1 in improving quality of life in patients with chronic constipation

  2021cites this paper
• Parkinson’s disease patients’ short chain fatty acids production capacity after in vitro fecal fiber fermentation

  2021cites this paper
• Protective effects of Bacillus subtilis ASAG 216 on growth performance, antioxidant capacity, gut microbiota and tissues residues of weaned piglets fed deoxynivalenol contaminated diets.

  2021cites this paper
• Efficacy of Bifidobacterium bifidum G9-1 in improving quality of life in patients with chronic constipation: a prospective intervention study

  2021cites this paper
• normocaloric or obesogenic diets for rats: effects on intestinal

  2020cites this paper
• Lubiprostone in patients with non-alcoholic fatty liver disease: a randomised, double-blind, placebo-controlled, phase 2a trial.

  2020cites this paper
• Exploratory studies from the PiPS trial fail to find evidence that Bifidobacterium breve BBG-001 modifies intestinal barrier function.

  2020cites this paper
• Safety and functional enrichment of gut microbiome in healthy subjects consuming a multi-strain fermented milk product: a randomised controlled trial

  2020cites this paper