Effect of adiponectin on bovine granulosa cell steroidogenesis, oocyte maturation and embryo development

BackgroundAdiponectin is an adipokine, mainly produced by adipose tissue. It regulates several reproductive processes. The protein expression of the adiponectin system (adiponectin, its receptors, AdipoR1 and AdipoR2 and the APPL1 adaptor) in bovine ovary and its role on ovarian cells and embryo, remain however to be determined.MethodsHere, we identified the adiponectin system in bovine ovarian cells and embryo using RT-PCR, immunoblotting and immunohistochemistry. Furthermore, we investigated in vitro the effects of recombinant human adiponectin (10 micro g/mL) on proliferation of granulosa cells (GC) measured by [3H] thymidine incorporation, progesterone and estradiol secretions measured by radioimmunoassay in the culture medium of GC, nuclear oocyte maturation and early embryo development.ResultsWe show that the mRNAs and proteins for the adiponectin system are present in bovine ovary (small and large follicles and corpus luteum) and embryo. Adiponectin, AdipoR1 and AdipoR2 were more precisely localized in oocyte, GC and theca cells. Adiponectin increased IGF-1 10(-8) M-induced GC proliferation (P < 0.01) but not basal or insulin 10(-8) M-induced proliferation. Additionally, adiponectin decreased insulin 10(-8) M-induced, but not basal or IGF-1 10(-8) M-induced secretions of progesterone (P < 0.01) and estradiol (P < 0.05) by GC. This decrease in insulin-induced steroidogenesis was associated with a decrease in ERK1/2 MAPK phosphorylation in GC pre-treated with adiponectin. Finally, addition of adiponectin during in vitro maturation affected neither the percentage of oocyte in metaphase-II nor 48-h cleavage and blastocyst day 8 rates.ConclusionsIn bovine species, adiponectin decreased insulin-induced steroidogenesis and increased IGF-1-induced proliferation of cultured GC through a potential involvement of ERK1/2 MAPK pathway, whereas it did not modify oocyte maturation and embryo development in vitro.

• Publication year

  2010

• Venue

  Reproductive Biology and Endocrinology

• Publication date

  2010-03-10

• Fields of study

  Agricultural and Food Sciences, Medicine, Biology

• Identifiers
  DOI 10.1186/1477-7827-8-23PMID 20219117PMCID 2845137
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2005influential reference
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  2005cited by this paper
• Adipokines: implications for female fertility and obesity.

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  2002cited by this paper
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  2002cited by this paper
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  2002cited by this paper
• Role of ERK1/2 in the differential synthesis of progesterone and estradiol by granulosa cells.

  2001cited by this paper
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  2000cited by this paper
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  1999cited by this paper
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  1999cited by this paper
• Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity.

  1999cited by this paper
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  1997cited by this paper
• Effects of insulin, insulin-like growth factor I, and gonadotropins on bovine granulosa cell proliferation, progesterone production, estradiol production, and(or) insulin-like growth factor I production in vitro.

  1993cited by this paper
• Basic local alignment search tool.

  1990cited by this paper
• Partitioning of nutrients during pregnancy and lactation: a review of mechanisms involving homeostasis and homeorhesis.

  1980cited by this paper
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