Specific binding of K- and I-region products of the H-2 complex to activated thymus-derived (T) cells belonging to different Ly subclasses

Responder cells [C57BL/6J X A.TL)F1 lymph node cells depleted of bursa equivalent-derived (B) cells by filtration through nylon wool columns] were activated against incompatible K-region and I-region products together under conditions where these antigens are presented on separate stimulator cells. The resulting T blasts were stained with different concentrations of antisera directed against incompatible stimulator K-region or I-region products, or both. We obtained results that strongly suggest that in these cultures each activated responder blast stains with antiserum directed against either K-region or I- region products, but not both. Responder blasts from the same cultures were treated with antiserum and complement (C) directed against either Ly-1.2 or Ly-2.2 T-cell-specific surface antigens. Anti-Ly-1.2 serum and C specifically eliminates virtually all responder blasts staining with antiserum directed against stimulator I-region products; whereas anti-Ly-2.2 serum reduces to background levels the proportion of cells staining with antiserum against stimulator K-region products. The results obtained suggest that T cells binding stimulator K-region and I- region products, respectively, belong to two different subclasses distinguishable by their Ly phenotypes. Possible explanations for this association of T- cell subclass and specificity are discussed.

• Publication year

  1976

• Venue

  Journal of Experimental Medicine

• Publication date

  1976-12-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/JEM.144.6.1545PMID 137268PMCID 2190479
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Further miniaturization and automation of in vitro lymphocyte cultures.

  2008cited by this paper
• B and T Cells in Immune Recognition

  1978cited by this paper
• Specific binding of alloantigens to T cells activated in the mixed lymphocyte reaction

  1976cited by this paper
• Specific adsorption of IgM antibody onto H-2-activated mouse T lymphocytes

  1976cited by this paper
• Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen

  1975influential reference
• Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity

  1975influential reference
• Expression of T-cell differentiation antigens on effector cells in cell- mediated cytotoxicity in vitro. Evidence for functional heterogeneity related to the surface phenotype of T cells

  1975influential reference
• Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity.

  1975cited by this paper
• I REGION‐ASSOCIATED ANTIGEN SYSTEM (Ia) OF THE MOUSE H‐2 GENE COMPLEX: FURTHER DEFINITION OF Ia SPECIFICITIES WITH RESTRICTED ANTI‐Ia ANTISER,A

  1974cited by this paper
• Cell‐mediated cell lysis in vitro: genetic control of killer cell production and target specificities in the mouse

  1974cited by this paper
• Isolation of mouse thymus-derived lymphocyte specific surface antigens.

  1974cited by this paper
• A rapid method for the isolation of functional thymus‐derived murine lymphocytes

  1973cited by this paper
• A bipartite interpretation and tentative mapping of H-2-associated MLR determinants in the mouse.

  1973cited by this paper
• SEROLOGICALLY DEFINED AND LYMPHOCYTE-DEFINED COMPONENTS OF THE MAJOR HISTOCOMPATIBILITY COMPLEX IN THE MOUSE

  1972cited by this paper
• Genetic and Immunological Complexity of Major Histocompatibility Regions

  1972cited by this paper
• GENETIC LINKAGE RELATIONSHIPS OF LOCI SPECIFYING DIFFERENTIATION ALLOANTIGENS IN THE MOUSE

  1972cited by this paper
• The somatic generation of immune recognition

  1971cited by this paper
• Improved complementation in the cytotoxic test.

  1970cited by this paper

• The mosquito effect: regulatory and effector T cells acquire cytoplasmic material from tumor cells through intercellular transfer

  2023cites this paper
• The Intricate Behavior of T Cells

  2014cites this paper
• Receptor-mediated T cell absorption of antigen presenting cell-derived molecules.

  2011cites this paper
• On mechanisms of activation and restriction in T and B lymphocytes.

  2008cites this paper
• Capture of membrane components via trogocytosis occurs in vivo during both dendritic cells and target cells encounter by CD8+ T cells

  2007cites this paper
• A very rapid and simple assay based on trogocytosis to detect and measure specific T and B cell reactivity by flow cytometry

  2006cites this paper
• Molecular Transfers through Transient Lymphoid Cell-Cell Channels

  2006cites this paper
• Inhibition of secondary mouse mixed lymphocyte reaction by anti-Ia sera

  2005cites this paper
• Antigen-binding receptors on T cells from long-term MLR. Evidence of binding sites for allogeneic and self-MHC products

  2005cites this paper
• An Ia-positive mouse T-cell clone is functional in presenting antigen to other T cells

  2004cites this paper
• IN VITRO ANALYSIS OF ALLOGENEIC LYMPHOCYTE INTERACTION

  2003cites this paper
• HYBRID Ia MOLECULES ON RETICULUM CELL SARCOMAS

  2003cites this paper
• Intercellular transfer of antigen‐presenting cell determinants onto T cells: molecular mechanisms and biological significance

  2002cites this paper
• Absorption and Internalization of Ligands Role of the Actin Cytoskeleton in T Cell

  2001cites this paper
• Role of the Actin Cytoskeleton in T Cell Absorption and Internalization of Ligands from APC1

  2001cites this paper
• T Cells Can Use Either T Cell Receptor or Cd28 Receptors to Absorb and Internalize Cell Surface Molecules Derived from Antigen-Presenting Cells

  2000cites this paper
• Effects of cytokines and glucocorticoids on endogenous class II MHC antigen expression by activated rat CD4+ T cells. Mature CD4+ CD8- thymocytes are phenotypically heterogeneous on activation.

  1996cites this paper
• Soluble factors in tolerance and contact sensitivity to DNFB in mice. X. IL-2 is the activation signal mediating release of synthesized suppressor factor.

  1991cites this paper
• ANTIGENICITY OF PASSIVELY ACQUIRED MAJOR HISTOCOMPATIBILITY ANTIGENS ON T CELLS

  1988cites this paper
• Identification of Transcripts of the T Cell Antigen Receptor β Chain Gene and Major Histocompatibility Complex Class II Genes in Antigen‐Presenting Cloned BK‐BI‐2.6.C6 Cells

  1988cites this paper
• The induction of cytotoxic T-cell responses with H-2 antigens shed from viable lymphocytes.

  1987cites this paper
• Soluble factors in tolerance and contact sensitivity to DNFB in mice. VII. Characterization of a monoclonal, efferent-acting suppressor factor with specificity for DNP/H-2Kd.

  1987cites this paper
• MHC Restricted Recognition by Cloned T Cells

  1986cites this paper
• Cloned murine Ia+ BK-BI-2.6.C6 T cells function as accessory cells presenting protein antigens to long-term-cultured antigen-specific T cell lines.

  1986cites this paper
• Search for class II major histocompatibility complex molecular involvement in the response of Lyt‐2+cytotoxic T lymphocyte precursors to alloantigen

  1985cites this paper
• Effects of Recombinant Interferon‐γ on HLA‐DR Antigen Shedding by Human Peripheral Blood Adherent Mononuclear Cells

  1985cites this paper
• Endogenous synthesis of Ia antigens by a cloned T cell line.

  1984cites this paper
• Immunoregulation by mouse T-cell clones. I. Suppression and amplification of cytotoxic responses by cloned H-Y-specific cytolytic T lymphocytes.

  1984cites this paper
• CONTROL OF CLONED ALLOREACTIVE T LYMPHOCYTE PROLIFERATIVE RESPONSES: A POSSIBLE ROLE FOR CELL‐SURFACE-BOUND ALLOANTIGEN

  1984cites this paper
• Cloned human T cells synthesize Ia molecules and can function as antigen presenting cells.

  1984cites this paper
• Isolation and analysis of the murine Ia molecular complex.

  1984cites this paper
• Multiple human Ia antigen molecules are expressed on functional T cell clones.

  1984cites this paper
• Suppressor T cell circuits in contact sensitivity. II. Induction and characterization of an efferent-acting, antigen-specific, H-2-restricted, monoclonal T cell hybrid-derived suppressor factor specific for DNFB contact hypersensitivity.

  1984cites this paper
• Flow cytometric analysis of specific binding of soluble Ia by I-region restricted alloactivated T cells.

  1984cites this paper
• The role of T cell subsets in the generation of secondary cytolytic responses in vitro against class I and class II major histocompatibility complex antigens.

  1984cites this paper
• T cell responses induced by the parenteral injection of antigen-modified syngeneic cells. I. Induction, characterization, and regulation of antigen-specific T helper cells involved in delayed-type hypersensitivity responses.

  1983cites this paper
• Anti-Lyt-1 monoclonal antibody inhibits induction of anti-self-TNP but not of alloreactive cytotoxic T lymphocytes.

  1983cites this paper
• Clonal analysis of B and T cell responses to Ia antigens. IV. Proliferative T cell clones recognizing E beta and/or E alpha allodeterminants.

  1983cites this paper
• Cytotoxic T‐Lymphocytes How Do They Function?

  1983cites this paper
• Specific lysis of antigenically irrelevant cells by cytotoxic T lymphocytes upon insertion of appropriate antigens into the target cell plasma membranes.

  1983cites this paper
• The role of Ia molecules in the activation of T lymphocytes. II. Ia-restricted recognition of allo K/D antigens is required for class I MHC-stimulated mixed lymphocyte responses.

  1983cites this paper
• Response against single minor histocompatibility antigens. I. Functional and immunogenetic analysis of cloned cytolytic T cells.

  1983cites this paper
• Cytolytic T lymphocytes.

  1983cites this paper
• T CELL HELP IN CYTOTOXIC T LYMPHOCYTE RESPONSES: ROLE OF THE I REGION IN HELPER CELL INDUCTION

  1983cites this paper
• The nature of the interaction between suppressor and helper T cells in the response to LDHB.

  1983cites this paper
• Role of Ia antigens in graft vs. host reactions. II. Molecular and functional analysis of T cell alloreactivity by the characterization of host Ia antigens on alloactivated donor T cells

  1982cites this paper
• Regulation of in vitro cytotoxic T lymphocyte generation. II. Demonstration of noncytotoxic alloantigen‐specific suppressor T lymphocytes

  1982cites this paper
• The biological and biochemical basis of allogeneic effect factor (AEF) activity: relationship to T cell alloreactivity.

  1982cites this paper
• Anti-hapten antibody in primary immune antiserum can specifically inhibit antibody-secreting cells.

  1982cites this paper
• I-A antigens on cloned alloreactive murine T lymphocytes are acquired passively.

  1982cites this paper
• ABSORPTION OF SHED I‐Ak AND H‐2Kk ANTIGENS BY LYMPHOID CELLS

  1982cites this paper
• Recruitment of functional subpopulations of T lymphocytes Initiator T lymphocytes recruit helper T cells for cytotoxic response

  1982cites this paper
• Functional and antigenic properties of cultured T cells in the cell mediated lympholysis (CML) assay.

  1982cites this paper
• Role of I-region gene products in T cell activation. I. Stimulation of T lymphocyte proliferative responses by subcellular membrane preparations containing Ia alloantigens.

  1982cites this paper
• T cell-mediated immunity in malaria. I. The Ly phenotype of T cells mediating resistance to Plasmodium yoelii.

  1982cites this paper
• The nature of allorosette-forming cells in rats

  1982cites this paper
• Cytolytic T lymphocyte responses to metabolically inactivated stimulator cell. I. Metabolic inactivation impairs both CD and LD antigen signals.

  1982cites this paper
• Interleukin 2 is not sufficient as helper component for the activation of cytotoxic T lymphocytes but synergizes with a late helper effect that is provided by irradiated I-region-incompatible stimulator cells.

  1982cites this paper
• The generation of cytolytic activity in mixed leukocyte cultures: cortisone-resistant thymocytes are deficient in helper T lymphocytes.

  1981cites this paper
• Alteration of allospecific t-cell receptors after differentiation from prethymic precursor cells in semiallogeneic environments.

  1981cites this paper
• In vitro analysis of allogeneic lymphocyte interaction. V. Identification and characterization of two components of allogeneic effect factor, one of which displays H-2-restricted helper activity and the other, T cell-growth factor activity

  1981cites this paper
• Enhancement of T. lymphocyte functions by Fc fragments of immunoglobulin. II. Augmentation of the cell-mediated lympholysis response occurs through an Lyt-1+2- helper T cell.

  1981cites this paper
• H‐2 Restricted Cytolytic and Noncytolytic T Cell Clones: Isolation, Specificity and Functional Analysis

  1981cites this paper
• Cellular expression and molecular structure of Ia antigens on human lymphocytes: an overview.

  1981cites this paper
• The T-cell receptor problem.

  1981cites this paper
• Lyt phenotype analysis of the effector cells responsible for evoking lymphocyte-induced angiogenesis (LIA).

  1981cites this paper
• Allospecific T cells.

  1981cites this paper
• Biochemical characterization of Ia alloantigens in guinea pigs. I. Synthesis of Ia antigens by subsets enriched for B cells, T cells, or macrophages.

  1981cites this paper
• Expression of hybrid Ia molecules on the cell surface of reticulum cell sarcomas that are undetectable on host SJL/J lymphocytes

  1981cites this paper
• Enumeration of alloreactive helper T lymphocytes which cooperate with cytolytic T lymphocytes

  1981cites this paper
• Receptor specificity, functional characteristics, and cell-surface phenotype of a highly selected anti-I-Ab-specific, long-term T-cell line

  1981cites this paper
• Lyt phenotypes of primary cytotoxic T cells generated across the A and E region of the H‐2 complex

  1981cites this paper
• I-Kk and H-2Kk antigens are shed as supramolecular particles in association with membrane lipids.

  1981cites this paper
• Antigen‐Reactive Cell Opsonization (ARCO) and its Role in Antibody‐Mediated Immune Suppression

  1980cites this paper
• The role of anti-H-2K and H-2D alloantibodies in enhancement and acute antibody-mediated rejection of mouse skin allografts.

  1980cites this paper
• An Ia+ subpopulation of activated spleen T cells is generated by concanavalin A stimulation.

  1980cites this paper
• The role of the major histocompatibility gene complex in murine cytotoxic T cell responses.

  1980cites this paper
• Ia antigens on alloreactive T cells in man detected by monoclonal antibodies. Evidence for synthesis of HLA-D/DR molecules of the responder type.

  1980cites this paper
• Expression of Ia Antigens on T Lymphocytes

  1980cites this paper
• Role of the H-2I region in the generation of an antiviral cytotoxic T-cell response in vitro.

  1980cites this paper
• T‐T Cell Interactions during Cytotoxic T Lymphocyte (CTL) Responses: T Cell Derived Helper Factor (Interleukin 2) as a Probe to Analyze CTL Responsiveness and Thymic Maturation of CTL Progenitors

  1980cites this paper
• Alloactivated Lyt 1 +2− T lymphoblasts bind syngeneic Ia antigens

  1980cites this paper
• Priming against Stimulator‐derived Alloantigens Bound onto MLC‐activated Blasts

  1980cites this paper
• Host cell analysis of a rapidly metastasizing mouse tumor and derived low-metastatic variant lines.

  1980cites this paper
• Distinct effects of T cell growth factors and thymic epithelial factors on the generation of cytotoxic T lymphocytes by thymocyte subpopulations.

  1980cites this paper
• [Primary and secondary mixed lymphocyte reactions in mice (author's transl)].

  1980cites this paper
• Contemporary Topics in Immunobiology

  1980cites this paper
• Surface receptors on lymphoreticular cells: sensory devices for host recognition of foreign antigens and neoplasia.

  1980cites this paper
• Alloreactive cloned T cell lines. I. Interactions between cloned amplifier and cytolytic T cell lines

  1980cites this paper
• Regulation of immune response in allogeneic mixed spleen cell cultures. I. Influence of I-region on the generation of suppressor cells.

  1980cites this paper
• Idiotypes, T-cell receptors, and T-B cooperation.

  1980cites this paper
• I-REGION CONTROL OF THE ACTIVITY OF ALLOGENEIC EFFECT FACTOR1

  1979cites this paper
• The role of H-2 and Ia antigens in graft-versus-host reactions (GVHR). Presence of host alloantigens on donor cells after GVHR and suppression of GVHR with an anti-Ia antiserum against hose Ia antigens

  1979influential citation
• Increase in the percentage of sIgD-bearing lymphocytes during human mixed lymphocyte reaction.

  1979cites this paper
• Delayed-type hypersensitivity to allogeneic cells in mice. III. Sensitivity to cell-surface antigens coded by the major histocompatibility complex and by other genes

  1979cites this paper
• Idiotypic determinants on T-cell subpopulations

  1979cites this paper
• In vitro analysis of allogeneic lymphocyte interaction. IV. Dual recognition of B cell-associated Mls locus and I-region determinants by a helper allogeneic effect factor (AEF) generated across a minor H locus disparity.

  1979cites this paper
• Human lymphocyte surface markers after allogeneic activation: increase of Fc receptors-bearing cells.

  1979cites this paper
• Induction of cell-mediated cytotoxicity by lipoprotein containing histocompatibility antigens.

  1979cites this paper
• Modulation of immunity by antibody, antigen-antibody complexes and antigen.

  1979cites this paper