Live imaging and modeling of inner nuclear membrane targeting reveals its molecular requirements in mammalian cells

A new reporter allowing real-time imaging of membrane protein transport from the ER to the inner nuclear membrane (INM) reveals that targeting of INM proteins depends on the number and permeability of nuclear pores and the availability of nuclear binding sites.

• Publication year

  2015

• Venue

  Journal of Cell Biology

• Publication date

  2015-06-08

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/jcb.201409133PMID 26056140PMCID 4460149
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Nuclear Pore Scaffold Structure Analyzed by Super-Resolution Microscopy and Particle Averaging

  2013cited by this paper
• Fiji: an open-source platform for biological-image analysis

  2012cited by this paper
• The C-terminal domain of Nup93 is essential for assembly of the structural backbone of nuclear pore complexes

  2012cited by this paper
• Outfits for different occasions: tissue-specific roles of Nuclear Envelope proteins.

  2012cited by this paper
• The human nuclear pore complex as revealed by cryo-electron tomography.

  2012cited by this paper
• Lamin B Receptor Recognizes Specific Modifications of Histone H4 in Heterochromatin Formation*

  2012influential reference
• Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway

  2012cited by this paper
• Targeting of the SUN protein Mps3 to the inner nuclear membrane by the histone variant H2A.Z

  2011influential reference
• Long Unfolded Linkers Facilitate Membrane Protein Import Through the Nuclear Pore Complex

  2011cited by this paper
• System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics

  2011cited by this paper
• Traversing the NPC along the pore membrane

  2011cited by this paper
• Systems survey of endocytosis by multiparametric image analysis

  2010cited by this paper
• A classical NLS and the SUN domain contribute to the targeting of SUN2 to the inner nuclear membrane

  2010cited by this paper
• Automatic identification and clustering of chromosome phenotypes in a genome wide RNAi screen by time-lapse imaging.

  2010cited by this paper
• CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging

  2010cited by this paper
• The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex

  2010influential reference
• Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes

  2010cited by this paper
• Characterisation of the passive permeability barrier of nuclear pore complexes

  2009influential reference
• Baculovirus Data Suggest a Common but Multifaceted Pathway for Sorting Proteins to the Inner Nuclear Membrane

  2008cited by this paper
• Activation of cap-independent translation by variant eukaryotic initiation factor 4G in vivo.

  2008cited by this paper
• A drug-controllable tag for visualizing newly synthesized proteins in cells and whole animals

  2008cited by this paper
• Work Flow for Multiplexing siRNA Assays by Solid-Phase Reverse Transfection in Multiwell Plates

  2008cited by this paper
• Early sorting of inner nuclear membrane proteins is conserved

  2007cited by this paper
• Highway to the inner nuclear membrane: rules for the road

  2007cited by this paper
• Monitoring the permeability of the nuclear envelope during the cell cycle.

  2006cited by this paper
• Importin-α-16 is a translocon-associated protein involved in sorting membrane proteins to the nuclear envelope

  2006cited by this paper
• Karyopherin-mediated import of integral inner nuclear membrane proteins

  2006cited by this paper
• The laminopathies: the functional architecture of the nucleus and its contribution to disease.

  2006cited by this paper
• Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore

  2004influential reference
• Nuclear Pore Complex Structure and Dynamics Revealed by Cryoelectron Tomography

  2004cited by this paper
• Cotranslational integration and initial sorting at the endoplasmic reticulum translocon of proteins destined for the inner nuclear membrane.

  2004cited by this paper
• Trafficking of ODV-E66 is mediated via a sorting motif and other viral proteins: facilitated trafficking to the inner nuclear membrane.

  2004cited by this paper
• Nucleoporins as components of the nuclear pore complex core structure and Tpr as the architectural element of the nuclear basket.

  2004cited by this paper
• The conserved Nup107-160 complex is critical for nuclear pore complex assembly.

  2003cited by this paper
• An NS3 protease inhibitor with antiviral effects in humans infected with hepatitis C virus

  2003influential reference
• Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo.

  2003cited by this paper
• An NS 3 protease inhibitor with antiviral effects in humans infected with hepatitis C virus

  2003influential reference
• Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina.

  2002cited by this paper
• Kinetic analysis of translocation through nuclear pore complexes

  2001influential reference
• Integral Membrane Proteins of the Nuclear Envelope Are Dispersed throughout the Endoplasmic Reticulum during Mitosis

  1997cited by this paper
• Nuclear Membrane Dynamics and Reassembly in Living Cells: Targeting of an Inner Nuclear Membrane Protein in Interphase and Mitosis

  1997cited by this paper
• Signals and structural features involved in integral membrane protein targeting to the inner nuclear membrane

  1995influential reference
• Primary structure analysis and lamin B and DNA binding of human LBR, an integral protein of the nuclear envelope inner membrane.

  1994cited by this paper
• The first membrane spanning region of the lamin B receptor is sufficient for sorting to the inner nuclear membrane

  1993cited by this paper
• Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components

  1990cited by this paper
• Internuclear exchange of an inner nuclear membrane protein (p55) in heterokaryons: in vivo evidence for the interaction of p55 with the nuclear lamina

  1990cited by this paper

• C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin

  2025cites this paper
• C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin

  2025cites this paper
• The endoplasmic reticulum connects to the nucleus by constricted junctions that mature after mitosis

  2024cites this paper
• Werner syndrome RECQ helicase participates in and directs maintenance of the protein complexes of constitutive heterochromatin in proliferating human cells

  2024cites this paper
• Sculpting nuclear envelope identity from the endoplasmic reticulum during the cell cycle

  2024cites this paper
• Unveiling the Dynamics: A Comprehensive Review of Biological Membrane Kinetics During Cell Division

  2024cites this paper
• Dynamic remodelling of the endoplasmic reticulum for mitosis.

  2024cites this paper
• A membrane-sensing mechanism links lipid metabolism to protein degradation at the nuclear envelope

  2023cites this paper
• The endoplasmic reticulum connects to the nucleus by constricted junctions that mature after open mitosis in mammalian cells

  2023cites this paper
• The Nuclear Pore Complex: Birth, Life, and Death of a Cellular Behemoth

  2022cites this paper
• Does the nuclear envelope retain its identity during mitosis?

  2022cites this paper
• Dynamic quality control machinery that operates across compartmental borders mediates the degradation of mammalian nuclear membrane proteins

  2022cites this paper
• Making and breaking the inner nuclear membrane proteome.

  2022cites this paper
• A membrane sensing mechanism couples local lipid metabolism to protein degradation at the inner nuclear membrane

  2022cites this paper
• A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents

  2022cites this paper
• The Diverse Cellular Functions of Inner Nuclear Membrane Proteins.

  2021cites this paper
• Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation

  2021cites this paper
• Nuclear pores dilate and constrict in cellulo

  2021cites this paper
• Components and Mechanisms of Nuclear Mechanotransduction.

  2021cites this paper
• Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time

  2021cites this paper
• Nuclear pores constrict upon energy depletion

  2020cites this paper
• TMEM147 interacts with lamin B receptor, regulates its localization and levels, and affects cholesterol homeostasis

  2020cites this paper
• Quality Control of Protein Complex Assembly by a Transmembrane Recognition Factor

  2020cites this paper
• Membrane and organelle dynamics during cell division

  2020cites this paper
• Lipid and protein dynamics that shape nuclear envelope identity

  2020cites this paper
• Nucleoplasmic signals promote directed transmembrane protein import simultaneously via multiple channels of nuclear pores

  2020cites this paper
• The Cell Nucleus and Its Compartments

  2020cites this paper
• Torsin ATPases influence chromatin interaction of the Torsin regulator LAP1

  2020cites this paper
• REEP4 is recruited to the inner nuclear membrane by ELYS and promotes nuclear pore complex formation

  2020cites this paper
• GPU-accelerating ImageJ Macro image processing workflows using CLIJ

  2020cites this paper
• Casting a Wider Net: Differentiating between Inner Nuclear Envelope and Outer Nuclear Envelope Transmembrane Proteins

  2019cites this paper
• Targeting of LRRC59 to the Endoplasmic Reticulum and the Inner Nuclear Membrane

  2019cites this paper
• A nuclear localization signal targets tail-anchored membrane proteins to the inner nuclear envelope in plants

  2019cites this paper
• Methodologies to monitor protein turnover at the inner nuclear membrane.

  2019cites this paper
• Structure and Assembly of the Nuclear Pore Complex.

  2019cites this paper
• Patrolling the nucleus: inner nuclear membrane-associated degradation

  2019cites this paper
• STRAIN-SPECIFIC PROTEIN INTERACTION AND LOCALIZATION OF TWO STRAINS OF POTATO YELLOW DWARF VIRUS AND FUNCTIONAL DOMAINS OF THEIR MATRIX PROTEIN

  2019cites this paper
• Selective clearance of the inner nuclear membrane protein emerin by vesicular transport during ER stress

  2019influential citation
• Measurements of Intensity Dynamics at the Periphery of the Nucleus

  2019cites this paper
• Flexible and Extended Linker Domains Support Efficient Targeting of Heh2 to the Inner Nuclear Membrane.

  2019cites this paper
• Nuclear-Cytoplasmic Transport

  2018cites this paper
• Fluorescent Labeling of the Nuclear Envelope by Localizing Green Fluorescent Protein on the Inner Nuclear Membrane.

  2018cites this paper
• Conservation of inner nuclear membrane targeting sequences in mammalian Pom 121 and yeast Heh 2 membrane

  2018cites this paper
• The Dynamic Nature of the Nuclear Envelope.

  2018cites this paper
• Navigating the Nuclear Envelope: One or Multiple Transport Mechanisms for Integral Membrane Proteins?

  2018cites this paper
• The Molecular Composition and Function of the Nuclear Periphery and Its Impact on the Genome

  2018cites this paper
• Myosin‐1C uses a novel phosphoinositide‐dependent pathway for nuclear localization

  2018cites this paper
• Dynamics of sister chromatid resolution during cell cycle progression

  2018cites this paper
• Dynamics and Structure-Function Relationships of the Lamin B Receptor (LBR)

  2017cites this paper
• Lamin B Receptor: Interplay between Structure, Function and Localization

  2017cites this paper
• Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture

  2017cites this paper
• Mechanisms of ciliary targeting: entering importins and Rabs

  2017cites this paper
• Herpes Simplex Virus 1 UL34 Protein Regulates the Global Architecture of the Endoplasmic Reticulum in Infected Cells

  2017influential citation
• Cellular Reorganization during Mitotic Entry.

  2017cites this paper
• Genome sequence variation in the constricta strain dramatically alters the protein interaction and localization map of Potato yellow dwarf virus

  2017cites this paper
• Protein Targeting: ER Leads the Way to the Inner Nuclear Envelope.

  2017cites this paper
• Epilogue: A Framework for Bioimage Analysis

  2017cites this paper
• JCB_201606043 2827..2842

  2017cites this paper
• Mechanisms and functions of nuclear envelope remodelling

  2017cites this paper
• Efficient protein targeting to the inner nuclear membrane requires Atlastin-dependent maintenance of ER topology

  2017cites this paper
• Emery–Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane

  2016cites this paper
• A model for coordinating nuclear mechanics and membrane remodeling to support nuclear integrity.

  2016cites this paper
• ELYS regulates the localization of LBR by modulating its phosphorylation state

  2016cites this paper
• The Nuclear Pore Complex as a Flexible and Dynamic Gate.

  2016cites this paper
• New Insights into Mechanisms and Functions of Nuclear Size Regulation.

  2016cites this paper
• Dissecting Torsin/cofactor function at the nuclear envelope: a genetic study

  2016cites this paper
• Analysis of membrane proteins localizing to the inner nuclear envelope in living cells

  2016cites this paper
• Active Nuclear Import of Membrane Proteins Revisited

  2015cites this paper
• Establishment of NE asymmetry—targeting of membrane proteins to the inner nuclear membrane.

  2015cites this paper
• Gated entry into the ciliary compartment

  2015cites this paper
• University of Groningen Conservation of inner nuclear membrane targeting sequences in mammalian Pom121 and yeast Heh2 membrane proteins Kralt,

  2015cites this paper
• Conservation of inner nuclear membrane targeting sequences in mammalian Pom121 and yeast Heh2 membrane proteins

  2015cites this paper
• MIT Open Access Articles The Nuclear Pore Complex as a Flexible and Dynamic Gate

  year unknowncites this paper
• CELL BIOLOGY Nuclear pores dilate and constrict in cellulo

  year unknowncites this paper