SOME OBSERVATIONS ON NEGATIVELY STAINED MITOCHONDRIA

A particulate component of mitochondria is described which is observed in negatively stained preparations of unfixed whole mitochondria or their fragments. It consists of a roughly spherical particle, approximately 85 A in diameter, found on the surface of the cristae and probably also on the side of the mitochondrial envelope which faces the matrix. It is attached to the underlying membrane by a narrow stalk 40 to 50 A long. The particle appears to be rather labile and it is destroyed or at least rendered unrecognizable by all conventional fixatives used in electron microscopy, especially OsO(4).

• Publication year

  1963

• Venue

  Journal of Cell Biology

• Publication date

  1963-05-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.17.2.443PMID 13984235PMCID 2106204
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Cell‐Membrane Ultrastructure: Low‐Temperature Electron Microsopy and X‐Ray Diffraction Studies of Lipoprotein Components in Lamellar Systems

  1962cited by this paper
• [Morphological research on the erythrocyte membrane; electron microscopy of the surface spreading].

  1955cited by this paper
• AN ELECTRON MICROSCOPE STUDY OF THE MITOCHONDRIAL STRUCTURE

  1953cited by this paper
• Cytochemical studies of mammalian tissues; the isolation of cell components by differential centrifugation: a review.

  1951cited by this paper

• Mitochondrial Respiratory Chain Complexes.

  2018cites this paper
• Membrane Protein Complexes: Structure and Function

  2018cites this paper
• Electron microscopic visible ischemic changes of the mitochondrial ATPases in human myocardial cells during extracorporal circulation

  2005cites this paper
• Electron Microscopy of Structure to Function

  2004cites this paper
• Untersuchungen zur ultrastruktur von Microsporum gypseum

  2004cites this paper
• Untersuchungen zur Ultrastruktur von Microsporum gypseum

  2004cites this paper
• Chromosome fibers studied by a spreading technique

  2004cites this paper
• Electron microscopic observations on isolated mitochondrial membranes, prepared by various histological methods

  2004cites this paper
• Die Ultrastruktur der Zellwand und des Chloroplasten von Chlorella

  2004cites this paper
• Electron microscopy of the F1F0 ATP synthase: From structure to function

  1994cites this paper
• Citation life cycles of ten citation classics

  1991cites this paper
• Molecular structure of mitochondrial cristae, solid state biochemistry and a simple theory of respiration-phosphorylation coupling.

  1991cites this paper
• The Anatomy of Biological Interfaces

  1986cites this paper
• Are there knobs on energy transducing membranes in situ?

  1985cites this paper
• A biochemical and structural fractionation of the human red blood cell membrane using potassium phosphotungstate.

  1980cites this paper
• Electron microscopic studies of various cells in the alveolar wall of mice with special reference to spheroid alveolar epithelial cells after intravenous injection of squid-ink (sepia-melanin) solution.

  1977cites this paper
• Electron Microscopic Methods in Membrane Biology

  1976cites this paper
• Ultrastructural changes in hepatocytes during the development of experimental cirrhosis of the liver in rats

  1975cites this paper
• Effect of phosphotungstic acid on the release by AMP of the controlled respiration of rat liver mitochondria.

  1973cites this paper
• [The ultrastructure of yeast cell mitochondria revealed by the freeze etching method].

  1973cites this paper
• A METHOD FOR INCREASING CONTRAST OF MITOCHONDRIAL INNER MEMBRANE SPHERES IN THIN SECTIONS OF EPON-ARALDITE EMBEDDED TISSUE

  1973cites this paper
• The localization of mitochondrial creatine kinase, and its use for the determination of the sidedness of submitochondrial particles.

  1973cites this paper
• BIOCHEMISTRY OF MUSCLE MITOCHONDRIA

  1973cites this paper
• Die Zelle—Elementarorganismus oder Symbiose?

  1973cites this paper
• ULTRASTRUCTURAL STUDIES OF BEEF HEART MITOCHONDRIA

  1972cites this paper
• The mitochondrial membrane system.

  1972cites this paper
• Ion-Translocation in Energy-Conserving Membrane Systems

  1972cites this paper
• [Detection of elementary particles on mitochondrial internal membranes by the method of cryocleavage].

  1972cites this paper
• CHAPTER III – Organelle-Bound Enzymes

  1971cites this paper
• CHAPTER II – Cell Particles

  1971cites this paper
• A mitochondrial disease of leaf cells infected with an apple virus.

  1971cites this paper
• Structural Aspects of Cell Membranes

  1970cites this paper
• Effects of phosphotungstic acid and silicotungstic acid on respiration and integrity of rat liver mitochondria.

  1970cites this paper
• Topography of mitochondrial membranes in relation to metabolism.

  1970cites this paper
• Fine Structure of Mitochondria of Spirostomum ambiguum as Seen in Sectioned and Negatively‐Stained Preparations*

  1970cites this paper
• Purification and ultrastructural properties of the calcium accumulating membranes in isolated sarcoplasmic reticulum preparations from skeletal muscle

  1969cites this paper
• An electron microscope study of thoracic mitochondrial membranes in developing adults of the blowfly Lucilia cuprina

  1969cites this paper
• Modern Views on the Physical Structure of the Membrane of the Fat Globules in Milk and Cream and a Possible Relation with the Migration of Copper During Butter Manufacture

  1969cites this paper
• A quantitative study of myocardial mitochondria in experimental cardiac hypertrophy.

  1969cites this paper
• [Ultrastructure of the mitochondrial membrane. Study by negative staining and cryo-fixation].

  1969cites this paper
• The significance of the structural and functional similarities of bacteria and mitochondria.

  1969cites this paper
• Funktionelle Morphologie der Zelle

  1968cites this paper
• On the structure of mitochondria and the value of ammonium molybdate as a negative stain for osmotically sensitive structures.

  1968cites this paper
• Ultrastructure of sonic and digitonin fragments from beef heart mitochondria.

  1968cites this paper
• CHAPTER 4 – Structure, Function and Developmental Changes in Mitochondria of Higher Plant Cells

  1968cites this paper
• Studies on the fine structure of the rat liver inner mitochondrial membranes negatively stained.

  1968cites this paper
• Membrane systems of mitochondria. VI. Membranes of liver mitochondria.

  1968cites this paper
• Studies on the electron transfer system. LXIX. "Solubilization" of the mitochondrial inner membrane by sonic oscillation.

  1968cites this paper
• The interpretation of freeze-etched artificial and bilogical membranes.

  1968cites this paper
• Die Orthologie und Pathologie der Zelle im elektronenmikroskopischen Bild

  1968cites this paper
• [Studies on the ultrastructure of Microsporum gypseum (Bodin 1907), Guiart and Grigoraki 1928. II. Structure and multiplication of mitochondria].

  1967cites this paper
• Ribosomes from rat liver mitochondria. I. Isolation procedure and contamination studies.

  1967cites this paper
• [Electron microscopic findings in 3 cases of chronic progressive ocular muscular dystrophy].

  1967cites this paper
• Mitochondrial oxidative phosphorylation.

  1967cites this paper
• Structural changes in swollen ascites tumor mitochondria.

  1967cites this paper
• A study of mitochondrial membranes in relation to elementary particles.

  1967cites this paper
• Mitochondrial oxidations and energy coupling.

  1967cites this paper
• Tubular systems in Purkinje cells of the cat heart.

  1967cites this paper
• The preparation and biochemical properties of mitochondria from Neurospora crassa.

  1967cites this paper
• Current techniques in biomedical electron microscopy.

  1967cites this paper
• Fine Structure of the Cytomembranes of Nitrosocystis oceanus

  1967cites this paper
• Microtubular elements and cell membranes isolated from nucleated erythrocytes.

  1967cites this paper
• Membrane lipids and drug action.

  1967cites this paper
• Histochemische Reaktion und „negative staining“ von Zellfraktionen

  1967cites this paper
• Ribosomes from rat liver mitochondira. II. Partial characterization.

  1967cites this paper
• Localized swelling of the inner compartment in negatively stained mitochondria.

  1967cites this paper
• [Histochemical reactions and "negative staining" of cellular fractions. I. Studies on the activity of acid phosphatase in spleen lysosomes].

  1967cites this paper
• [Electron microscopic demonstration of electron-dense globular particles on mitochondrial membranes of the rat liver in ultrathin sections].

  1967cites this paper
• Energy-Linked Reactions of Plant Mitochondria1

  1967cites this paper
• THE SUBMITOCHONDRIAL LOCALIZATION OF MONOAMINE OXIDASE

  1967cites this paper
• Oxidative Phosphorylierung in Mitochondrien

  1967cites this paper
• THE FINE STRUCTURE OF RENAL TUBULES IN CORTEX AND MEDULLA1

  1967cites this paper
• The mitochondrion.

  1967cites this paper
• CORRELATION OF MITOCHONDRIAL STRUCTURE AND FUNCTION

  1966cites this paper
• Biogenesis ommitochondria.

  1966cites this paper
• GRANULO‐FIBRILLAR AND GLOBULAR SUBSTRUCTURE IN UNIT MEMBRANES *

  1966cites this paper
• A COAT OF REPEATING SUBUNITS ON THE CYTOPLASMIC SURFACE OF THE PLASMA MEMBRANE IN THE RECTAL PAPILLAE OF THE BLOWFLY, CALLIPHORA ERYTHROCEPHALA (MEIG.), STUDIED IN SITU BY ELECTRON MICROSCOPY

  1966cites this paper
• Phospholipids and biomembranes

  1966cites this paper
• Biogenesis of Mitochondria

  1966cites this paper
• The membrane systems of the mitochondrion. II. The K fraction of the outer membrane of beef heart mitochondria.

  1966cites this paper
• Lipid-protein interactions and cohesional forces in the lipoproteins systems of membranes

  1966cites this paper
• CHAPTER 2 – Mitochondria

  1966cites this paper
• [Electron microscopic study on the action of surface-active substances on bacterial cell envelopes: 1. Experiments with cell walls of Proteus vulgaris and P. mirabilis].

  1966cites this paper
• The Procaryotic Photosynthetic Apparatus

  1966cites this paper
• The membrane systems of the mitochondrion

  1966cites this paper
• The membrane systems of the mitochondrion. I. The S fraction of the outer membrane of beef heart mitochondria.

  1966cites this paper
• Electronmicroscopic studies of isolated yeast mitochondria with negative staining and thin sectioning methods.

  1966cites this paper
• Membranes as expressions of repeating units.

  1966cites this paper
• CYTOCHEMISTRY OF PHOSPHATASES OF THE SARCOPLASMIC RETICULUM

  1966cites this paper
• Anhydrous ultrathin sectioning and staining for electron microscopy.

  1966cites this paper
• The membrane systems of the mitochondrion. III. The isolation and properties of the outer membrane of beef heart mitochondria.

  1966cites this paper
• A study of structure of the mitochondrial membrane system.

  1966cites this paper
• The demonstration of the elementary particles of mitochondrial membranes fixed with glutaraldehyde.

  1966cites this paper
• FINE STRUCTURAL CHANGES IN ISOLATED MITOCHONDRIA OF HEALTHY AND VIRUS-INFECTED VICIA FABA L.'

  1966cites this paper
• Elektronenmikroskopische Untersuchungen zur Wirkung von oberflächenaktiven Substanzen auf bakterielle Zellumhüllungen 1. Versuche mit Zellwänden von Proteus vulgaris und P. mirabilis

  1966cites this paper
• ELECTRON MICROSCOPY OF TWO SMALL SPHERICAL PLANT VIRUSES IN THIN SECTIONS

  1966cites this paper
• [Ultra-structure of the cell wall and chloroplast of Chlorella].

  1966cites this paper
• Role of lipids in the structure and function of biological membranes.

  1966cites this paper
• Two childhood myopathies with abnormal mitochondria. I. Megaconial myopathy. II. Pleoconial myopathy.

  1966cites this paper
• CHARACTERISTICS OF ISOLATED AND PURIFIED PREPARATIONS OF THE OUTER AND INNER MEMBRANES OF MITOCHONDRIA *

  1966cites this paper